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DOI: https://doi.org/10.17728/jatp.7442

Pengolahan Sinbiotik Kultur Campuran yang Berasal dari Kombinasi Bekatul Gandum sebagai Prebiotik dan Jus Kubis Terfermentasi sebagai Probiotik melalui Proses Fermentasi

*Cahya Setya Utama scopus  -  Laboratorium Teknologi Pakan, Departemen Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, Indonesia
Zuprizal Zuprizal scopus  -  Departemen Nutrisi dan Makanan Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia
Chusnul Hanim scopus  -  Departemen Nutrisi dan Makanan Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia
Wihandoyo Wihandoyo scopus  -  Departemen Produksi Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

Penelitian ini bertujuan untuk mengkaji kualitas sinbiotik kultur campuran yang berasal dari jus kubis terfermentasi sebagai probiotik yang ditambahkan pada bekatul gandum sebagai prebiotik melalui proses fermentasi. Penelitian ini dilakukan dengan menggunakan rancangan acak lengkap pola faktorial 3x3 dengan 3 ulangan. Faktor yang digunakan adalah lama pemeraman dan variasi konsentrasi jus kubis. Parameter yang diamati adalah komponen proksimat (kadar air, abu, lemak kasar, protein kasar, serat kasar dan bahan ekstrak tanpa nitrogen (BETN), komponen serat berupa acid detergent fibre atau ADF, neutral detergent fibre (NDF), selulosa, hemiselulosa dan lignin, serta gross energi, glukosa, sukrosa, mannosa, arabinosa, rafinosa, amilum, amilosa, amilopektin dan pati resisten serta profil sinbiotik kultur campuran melalui analisis scanning electron microscope (SEM). Hasil penelitian memperlihatkan adanya peningkatan yang signifikan pada kadar abu, protein kasar, serat kasar (p<0,05) sedangkan pada parameter BETN, ADF, NDF, hemiselulosa, lignin, gross energi, sukrosa, mannosa, arabinosa, rafinosa, amilosa, amilum, amilopektin dan pati resisten terdapat interaksi antar kedua faktor (p<0,05) namun pada parameter glukosa tidak terdapat interaksi antar kedua faktor. Kesimpulan penelitian yaitu sinbiotik kultur campuran terbaik terdapat pada penambahan 40% jus kubis terfermentasi dengan lama fermentasi 4 hari. Sinbiotik kultur campuran ini dapat digunakan sebagai sumber additive untuk pangan maupun kepentingan lainnya seperti pakan ternak.

Processing of Mixed Culture Sinbiotics Originating from the Combination of Wheat Pollard as Prebiotics and Fermented Cabbage Juice as Probiotics through the Fermentation Process

Abstract
The objective of this study was to examine the quality of mixed culture synbiotics derived from fermented cabbage juice as probiotics which were added to wheat pollard as a prebiotic through the fermentation process. The study used a completely randomized 3x3 factorial pattern design with 3 replications and the observed factors were duration of incubation and concentrations of applied cabbage juice. Proximate components (water content, ash, crude fat, crude protein, crude fiber and extraction material without nitrogen or BETN), fiber components (acid detergent fiber (ADF), neutral detergent fiber (NDF), cellulose, hemicellulose and lignin), gross energy, glucose, sucrose, mannose, arabinose, raffinose, starch, amylose, amylopectin and resistant starch and mix culture synbiotic profile through scanning electron microscope (SEM) were analyzed. The results showed a significant increase in ash content, crude protein, crude fiber (p<0.05) while in BETN, ADF, NDF parameters, hemicellulose, lignin, gross energy, sucrose, mannose, arabinose, raffinose, amylose, starch, amylopectin and resistant starch interaction between the two factors (p<0.05). However, in the glucose parameter there was no interaction between the two factors. The conclusion of the research is that the best mixed culture synbiotic is in the addition of 40% fermented cabbage juice with 4 days fermentation time. This mixed culture synbiotic can be used as an additive source for food and animal feed.

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Keywords: synbiotic; mix culture; cabbage waste; probiotics; fermentation; wheat pollard; prebiotics; sinbiotik; kultur campuran; kubis; probiotik; bekatul gandum; prebiotik

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  1. Abdel-Raheem, S.M., Abd-Allah, S.M.S., Hassanein, K.M.A. 2012. The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. International Journal for Agro Veterinary and Medical Sciences 6(4):277–289. DOI: 10.5455/ijavms.156
  2. Alloui, M.N., Szczurek, W.S., Tkiewicz, S.W. 2013. The usefulness of prebiotics and probiotics in modern poultry nutrition: Review. Annals of Animal Science 1:17–32. DOI: 10.2478/v10220-012-0055-x
  3. Association of Official Analytical Chemists (AOAC). 2006. Official Methods of Analysis, 18th ed. Association of Official Analytical Chemists, Washington, DC
  4. Baron, J.M. 1989. Friendly Bacteria. Reserved by the Roger Wyburn Mason and Jack M. Blount Foundation for Aradication of Rheumatoid Disease a.k.a The Arthritis Trust of America, US
  5. Bengmark, S. 2001. Pre, pro and symbiotics. Current Opinion in Clinical Nutrition and Metabolic Care 4(6): 571-579. DOI: 10.1097/00075197-200111000-00019
  6. Belitz, H.D, Grossch, W. 1999. Food Chemistry. Springer Verlag, Berlin
  7. Bird, A.R., Brown, I.L., Topping, D.L. 2000. Starches, resisten starches, the gut microflora and human health. Current Issues in Intestinal Microbiology 1(1):25-37. PMID:11709851
  8. Butt, H. 1999. Exploring management protocols for chronic fatique syndrome: a Case for pro and prebiotics. Probiotica. 8:2-6
  9. Carr, F., Chill, J.D., Maida, N. 2002. The lactic acid bacteria: A literature survey. Critical Reviews Microbiology. 28(4):281–370. DOI: 10.1080/1040-840291046759
  10. Charalampopoulos, D., Pandiella, S.S., Webb, C. 2002. Growth studies of potentially probiotic lactic acid bacteria in cereal – based substrates. Journal Applied Microbiology 92:851–859. DOI: 10.1046/j.1365-2672.2002.01592.x
  11. Cherney, D.J.R. 2000. Characterization of forages by chemical analysis. In: Forage evaluation in ruminant nutrition. CABI Publishing, New York
  12. Collins, M.D., Gibson, G.R. 1999. Probiotics, prebiotics, and symbiotics: approaches for modulating the microbial ecology of the gut. The American Journal of Clinical Nutrition 69(5):1052S-1057S. DOI: 10.1093/ajcn/69.5.1052s
  13. Crampton, E.W., Haris, L.E.1969. Applied Animal Nutrition. ed. 1st. The Engsminger Publishing Company, California
  14. Cristofaro, E., Motty, F., Wuhrmann, J.J.1994. Sugars in Nutrition. Academic Press. New York
  15. Eerlingen, R.C., Crombez, M., Delcour, J.A. 1993. Enzyme resisten starch I. quantitative and qualitative influence of incubation time and temperature of autoclaved starch on resisten starch pati formation. Journal Cereal Chemistry 70(3):339-344
  16. Fardiaz, S. 1992. Mikrobiologi Pangan. PT. Gramedia Pustaka Utama. Jakarta
  17. Giraud, G.R., Williams, C.M. 2000. Funtional Foods, Concept to Product. CRC Press, New York
  18. Goering, H.K., Van Soest, P.J. 1970. Forage Fiber Analysis. Agriculture Handbook No. 379. ARS USDA, Washington DC
  19. Goni, I.,G Diz, L., Manas, E., Calixto, F.S. 1996. Analysis of resistant starch: a method for foods and food products. Journal of Food Chemistry 56 (4):445-449. DOI: 10.1016/0308-8146(95)00222-7
  20. Hardy, H., Harris, J., Lyon, E., Beal, J., Foey, A. D. 2013. Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immunopathology. Nutrients. 5(6):1869-1912. DOI: 10.3390/nu5061869
  21. Hartadi, H., Reksohadiprodjo, S., Tilman, A. D.1997. Tabel komposisi bahan pakan untuk Indonesia. Gadjah Mada University Press, Yogyakarta
  22. Hodge, J. E., Osman, E. M.1976. Carbohydrate. Di dalam: Fennema, O.R. (ed.). Principles of food sciences. Marcel Dekker. Inc., New York
  23. Huang, D. P., Rooney, L.W.2001. Starches for snacks foods. Dalam Lusas, R.W., Rooney, L.W. (eds). 2001. Snack foods processing. CRC Press. New York
  24. Hustiany, R. 2006. Modifikasi asilasi dan suksinilasi pati tapioka sebagai bahan enkapsulasi komponen flavor. Disertasi, Institut Pertanian Bogor. Bogor
  25. Irma, S. R, Zubaida, E., Saparianti, E.2015. Evaluasi Pertumbuhan Isolat Probiotik (L. casei dan L. plantarum) dalam medium fermentasi berbasis ubi jalar (Ipomoea batatas L.) selama proses fermentasi (kajian jenis isolat dan jenis tepung ubi jalar). Jurnal Aplikasi Teknologi Pangan 4(4):133-141. DOI: 10.17728/jatp.v4i4.3
  26. Jung, G.H.J. 1997. Analysis of forage fiber and cell walls in ruminant nutrition. Journal of Nutrition 127:810–813. DOI: 10.1093/jn/127.5.810S
  27. Kearsly, M.W. 1988. Physical, Chemical and Biochemical Methods of Analysis of Carbohidrates. Analysis of Food Carbohydrates. Elsevier Applied Science Publisher Ltd., England
  28. Khalid, M.N., Elmer, H.M. 2008. Proteolytic activity by strains L. plantarum and L. casei. Journal of Dairy Science 73:3068—3076. DOI: 10.3168/jds.S0022-0302(90)78994-1
  29. Krabi, R.E, Assamoi, A.A., Ehon, F.A., Niamke. 2015. Screening of lactic acid bacteria as potential starter for the production of attieke, a fermented cassava food. Food and Environment Safety Journal 14(1):21-29
  30. Lewthwaite, S.L, Sutton, K.H., Triggs, C.M.1997. Free sugar composition of sweet potato cultivars after storage. New Zealand Journal of Crop and Horticultural Science 25:33-41. DOI: 10.1080/01140671.1997.9513984
  31. Li, X., Liu, L.Q., Xu, C.L. 2008. Effects of supplementation of fructo-oligosaccharide and/or Bacillus subtilis to diets on performance and intestinal microflora in broilers. Archiv für Tierzucht 51: 64-70. DOI: 10.5194/aab-51-64-2008
  32. Lu, C.D, Kawas, J.R., Maghoub, O.G. 2005. Fiber digestion and utilization in goats. Small Ruminant Research 60:45-65. DOI: 10.1016/j.smallrumres.2005.06.035
  33. Markowiak, P., Sli˙zewska, K. 2017. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients 9(9):1021. DOI: 10.3390/nu9091021
  34. Marsono, Y. 1999. Perubahan kadar resisten starch (RS) dan komposisi kimia beberapa bahan pangan kaya karbohidrat dalam proses pengolahan. Agritech 9(3):124-127. DOI: 10.22146/agritech.13729
  35. Miller, T.L., Wolin, J.M. 1996. Pathways of acetate, propionate, and butyrate formation by the human fecal microbial flora. Applied and Environmental Microbiology 62(5):1589 –1592. DOI: 10.1128/AEM.62.5.1589-1592
  36. Mookiah, S., Sieo, C.C., Ramasamy, K., Abdullah, N., Ho, Y.W. 2014. Effects of dietary prebiotics, probiotic and symbiotic on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of Science Food Agriculture 94(2): 341–348. DOI: 10.1002/jsfa.6365
  37. Murarolli, V.D.A., Burbarelli, M.F.C., Polycarpo, G.V., Ribeiro, P.A.P., Moro, M.E.G., Albuquerque, R. 2014. Prebiotic, probiotic and symbiotic as alternative to antibiotics on the performance and immune response of broiler chickens. Brazilian Journal of Poultry Science 16(3):279-284. DOI: 10.1590/1516-635x1603279-284
  38. Narasimha, G. Sridevi, A., Buddolla, V., Subhosh, C.M., Rajasekhar, R.D. 2006. Nutrient effects on productions of cellulolytic enzymes by Aspergillus niger. African Journal of Biotechnology 5:472-476
  39. National Research Council (NRC). 2001. Nutrient Requirement of Dairy Cattle. 7thed. National Academy Press, Washington, D.C
  40. Oberman, H. 1985. Fermented milks. In Microbiology of Fermented Foods. Vol. 1 Edited by J.B. Wood. Elsevier Appl. Sci. Publ., New York
  41. Pandey, K.R., Naik, S.R., Vakil, B.V.2015. Probiotics, prebiotics and synbiotics- a review. Journal of Food Science and Technology 52:7577–7587. DOI: 10.1007/s13197-015-1921-1
  42. Preston, T.R., Leng, R.A. 1987. Matching Ruminant Production System with Available Resources in the Tropics and Sub-tropics. Penambul Books, Australia
  43. Rackis, J.J. 1989. Physiological Effects of Food Carbohydrates. American Chemical Society, Washington D.C
  44. Ryan, S.M., Fitzgerald, G.F., Sinderen, D. 2006. Screening for and identification of starch-,amylopectin-, and pullulan-degrading activities in Bifidobacterial strains. Appl Environ Microbiol. 2006 Aug; 72(8): 5289–5296. DOI: 10.1128/AEM.00257-06
  45. Sajilata, M.G, Rekha, S.S., Kulkarni, P.R. 2006. Resisten starch – A review. Comprehensive reviews in food science and food safety 5(1):1-17. DOI: 10.1111/j.1541-4337.2006.tb00076.x
  46. Salminen, S., Wright, A.V.1993. Lactic Acid Bacteria: Microbiology and Functional Aspects. Marcel Dekker Inc., New York
  47. Sebayang, F. 2006. Pembuatan etanol dari molases secara fermentasi menggunakan sel Saccharomyces cerevisiae yang terimobilisasi pada kalsium alginat. Jurnal Teknologi Proses 5(2):75 – 80
  48. Steel, R.G.D., Torrie, J.H.1993. Prinsip dan prosedur statistik suatu pendekatan biometrik. Diterjemahkan oleh Bambang Sumantri. Cetakan ke- 4. Gramedia Pustaka Utama, Jakarta
  49. Sudarmadji, S., Haryono, B., Suhardi. 1984. Prosedur Analisa untuk Bahan Makanan dan Pertanian. Liberty, Yogyakarta
  50. Taggart, P. 2004. Starch as an Ingredients: Manufacture and Applications. In Ann Charlotte Eliasson (ed). Starch in Food: Structure, Function, and Applications. CRC Press, Florida
  51. Utama, C.S., Sulistiyanto, B., Kismiati, S. 2017. The effects of water addition and steaming duration on starch composition of wheatpollard. Reaktor 17(4):220-224. DOI: 10.14710/reaktor.17.4.221-225
  52. Utama, C.S., Zuprizal, Hanim, C., Wihandoyo. 2018a. Probiotic testing of Lactobacillus brevis and Lactobacillus plantarum from fermented cabbage waste juice. Pakistan Journal of Nutrition 17(7):323–328. DOI: 10.3923/pjn.2018.323.328
  53. Utama, C.S., Zuprizal, Hanim, C., Wihandoyo.2018b. Isolasi dan identifikasi bakteri asam laktat selulolitik yang berasal dari jus kubis terfermentasi. Jurnal Aplikasi Teknologi Pangan 7(1):1-6. DOI: 10.17728/jatp.2155
  54. Utama, C.S., Zuprizal, Hanim, C., Wihandoyo. 2019. Pengaruh lama pemanasan terhadap kualitas kimia wheat pollard yang berpotensi sebagai prebiotik. Jurnal Aplikasi Teknologi Pangan 8(3):113–123. DOI: 10.17728/jatp.5262
  55. Van Soest, P.J., Robertson, J.B., Lewis, B.A. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74:3583-3597. DOI: 10.3168/jds.S0022-0302(91)78551-2
  56. Wahyu J. 1994. Ilmu Nutrisi Unggas. Penerbit Gadjah Mada University Press. Yogyakarta
  57. Walter, H.G., Kohler, G.O. 1981. Treated and untreated cellulosic wastes as animal feeds: Recent work in the United States of America. Agriculture and Environment 6(2):229-243. DOI: 10.1016/0304-1131(81)90013-8
  58. Wigyanto, Suharjono, Novita. 2001. Pengaruh konsentrasi gula reduksi sari hati nanas dan inokulum Saccharomyces cerevisiae pada fermentasi etanol. Jurnal Teknologi Pertanian 2 (1):68–77
  59. Wurzburg, O. B. 1989. Modified starches. properties and uses. CRC Press, 5 Boca Raton, Florida
  60. Yoon, K.Y, Edward, E.W., Yong, D.H. 2004. Probiotication of tomato juice by lactic acid bacteria. Journal of Microbiology 42(4):315–318
  61. Zubaidah, E., Saparianti, E., Hindrawan, J. 2012. Studi aktivitas antioksidan pada bekatul dan susu skim terfermentasi probiotik Lactobacillus plantarum B2 dan Lactobacillus acidophilus. Jurnal Teknologi Pertanian 3(2):111-118

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