Formulasi dan Stabilitas Nanostructured Lipid Carrier dari Campuran Fraksi Stearin dan Olein Minyak Kelapa Sawit

*Miftakhur Rohmah -  Jurusan Teknologi Hasil Pertanian, Fakultas Pertanian, Universitas Mulawarman, Samarinda, Indonesia
Sri Raharjo -  Jurusan Teknologi Pengolahan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia
Chusnul Hidayat -  Jurusan Teknologi Pengolahan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia
Ronny Martien -  Departemen Farmasetika, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta, Indonesia
Received: 24 Nov 2018; Revised: 2 Feb 2019; Accepted: 25 Feb 2019; Published: 28 Feb 2019; Available online: 27 Feb 2019.
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Abstract

Nanostructured Lipid Carriers (NLC) adalah sistem pembawa berbasis lipida yang menggunakan kombinasi matriks berupa lipid padat dan cair yang distabilkan dengan penambahan surfaktan. NLC ini dikembangkan untuk memfasilitasi dispersi senyawa bioaktif hidrofobik dalam sistem hidrofilik.  Penelitian ini bertujuan untuk mendapatkan formulasi yang tepat untuk menghasilkan dan mengevaluasi stabilitas NLC yang menggunakan fraksi stearin dan fraksi olein minyak kelapa sawit sebagai lipid padat dan cair serta penambahan surfaktan Tween 80. Tahap pertama penelitian ini adalah membuat formula NLC dengan menggunakan metode High Shear Homogenisasi dan Ultrasonication dengan rasio lipid padat:lipid cair adalah 5:5 sd. 9:1% b/b, rasio lipid:surfaktan sebesar 1:3 sd. 1:5% b/b, dan rasio lipid + surfaktan/air adalah 65, 70, 80 dan 85% b/b. Tahap kedua adalah pengujian stabilitas NLC terhadap sentrifugasi, pemanasan pendinginan, dan penyimpanan pada suhu ruang selama 30 hari. Tahap ketiga yaitu karakterisasi NLC stabil meliputi nilai pH, vikositas, ukuran partikel, indeks polidispersi (PDI), potensial zeta (ZP) dan morfologi NLC. Hasil penelitian menunjukkan NLC stabil pada rasio lipid padat:lipid cair sebesar 5:5 sd. 9:1, rasio lipid:surfaktan adalah 1:4 sd.1:5, rasio lipid + surfaktan/air sebesar 65, 70, 75 dan 80%, pH pada kisaran 6,28±0,15 sd. 6,44±0,11, viskositas sebesar 18,17±0,29 sd. 26,83±1,61 cP, ukuran partikel sebesar 164,3±6,6 s.d. 340±2,0 nm, PDI sebesar 0,20±0,01 sd. 0,53±0,01, ZP sebesar (-21,25±0,01) sd. (-33,70±0,44) mV, dan memiliki morfologi bulat. Kesimpulannya, informasi tentang formulasi dan stabilitas NLC sebagai sistem pembawa senyawa bioaktif hidrophobik dapat digambarkan dengan baik.

Formulation and Stability of Nanostructured Lipid Carrier Prepared from a Mixture of Palm Stearin and Palm Olein

Abstract

Nanostructured Lipid Carriers (NLC) is a lipid-based delivery system using a matrix of solid and liquid lipids stabilized with surfactants, developed to facilitate the dispersion of hydrophobic bioactive compounds in hydrophilic systems. This study aimed to evaluate the formulation and stability of the NLC delivery system produced using palm stearin and palm olein as solid and liquid lipids as well as Tween 80 surfactant. As initial research, NLC formulations was done using the High Shear Homogenization and Ultrasonication using the ratio of solid:liquid lipids of 5:5 to 9:1% w/w, ratio lipid:surfactant as much as 1:3 to 1:5% w/w, and lipid+surfactant/water as much as 65, 70, 80 and 85% w/w. The second stage was testing the NLC stability against centrifugation, cooling, heating, and storage at room temperature for up to 30 days. The third stage was characterization of stable NLC i.e. pH, viscosity, particle size, polydispersion index (PDI), potential zeta (ZP), and NLC morphology. The results showed stable NLC at the ratio of solid:liquid lipids as much as 5:5 to 9:1, ratio of lipid:surfactant was 1:4 to 1:5, ratio of lipid+surfactant/water was 65, 70, 75 and 80%, pH value was 6.28±0.15 to 6.44±0.11, viscosity 18.17±0.29 to 26.83±1.61 cP, particle size was 164.3±6.6 to 340±2.0 nm, PDI was 0.20±0.01 to 0.53±0.01, ZP (-21.25±0.01) to (-33.70±0.44) mV, and had spherical morphology. As conclusion, the design of NLC as a delivery system for bioactive compounds was presented succesfully.

Keywords
Nanostructured Lipid Carriers; formulasi; stabilitas; fraksi stearin; fraksi olein

Article Metrics:

  1. Babazadeh, A., Ghanbarzadeh, B., Hamishehkar, H. 2017. Formulation of food grade nanostructured lipid carrier (NLC) for potential applications in medicinal-functional foods. Journal of Drug Delivery Science and Technology 39:50–58. DOI:10.1016/j.jddst.2017.03.001.
  2. Bagherpour, S., Alizadeh, A., Ghanbarzadeh, S., Mohammadi, M., Hamishehkar, H. 2017. Preparation and characterization of Betasitosterol-loaded nanostructured lipid carriers for butter enrichment. Food Bioscience 20:51–55. DOI:10.1016/j.fbio.2017.07.010.
  3. Danaei, M., Dehghankhold, M., Ataei, S., Hasanzadeh Davarani, F., Javanmard, R., Dokhani, A., Mozafari, M. 2018. Impact of particle size and polydispersity index on the clinical applications of lpidic nanocarrier systems. Pharmaceutics 10(2):2-17. DOI:10.3390/pharmaceutics 10020057.
  4. Fathi, H. A., Allam, A., Elsabahy, M., Fetih, G., El-Badry, M. 2018. Nanostructured lipid carriers for improved oral delivery and prolonged antihyperlipidemic effect of simvastatin. Colloids and Surfaces B: Biointerfaces 162:236–245. DOI:10.1016/j.colsurfb.2017.11.064.
  5. Han, F., Li, S., Yin, R., Liu, H., Xu, L. .2008. Effect of surfactants on the formation and characterization of a new type of colloidal drug delivery system: nanostructured lipid carriers. Colloids and Surfaces A: Physicochemical and Engineering Aspects 1–3(315):210–216. DOI:10.1016/ j.colsurfa.2007.08.005.
  6. How, C. W., Rasedee, A., Abbasalipourkabir, R. 2013. Characterization and cytotoxicity of nanostructured lipid carriers formulated with olive oil, hydrogenated palm oil, and polysorbate 80. IEEE Transactions on Nanobioscience 12(2):72–78. DOI:10.1109/TNB.2012.2232937.
  7. How, C. W., Abdullah, R., Abbasalipourkabir, R. 2011. Physicochemical properties of nanostructured lipid carriers as colloidal carrier system stabilized with polysorbate 20 and polysorbate. African Journal of Biotechnology 10(9):1684-1689. DOI: 10.5897/AJB10.1667.
  8. Hu, F. Q., Jiang, S. P., Du, Y. Z., Yuan, H., Ye, Y. Q., Zeng, S. 2006. Preparation and characteristics of monostearin nanostructured lipid carriers. International Journal of Pharmaceutics 314(1):83–89. DOI:10.1016/j.ijpharm.2006.01.040.
  9. Hung, L. C., Basri, M., Tejo, B. A., Ismail, R., Nang, H. L. L., Hassan, H. A., May, C. Y. 2011. An improved method for the preparations of nanostructured lipid carriers containing heat-sensitive bioactives. Colloids and Surfaces B: Biointerfaces 87(1):180–186. DOI:10.1016/j.colsurfb.2011.05.019.
  10. Karn-orachai, K., Smith, S. M., Phunpee, S., Treethong, A., Puttipipatkhachorn, S., Pratontep, S., Ruktanonchai, U. R. 2014. The effect of surfactant composition on the chemical and structural properties of nanostructured lipid carriers. Journal of Microencapsulation 31(6): 609–618. DOI: 10.3109/02652048.2014.911374.
  11. Lesmes, U., McClements, D. J. 2009. Structure–function relationships to guide rational design and fabrication of particulate food delivery systems. Trends in Food Science and Technology 20(10):448–457. DOI:10.1016/j.tifs.2009.05.006.
  12. Liu, Y., Wang, L., Zhao, Y., He, M., Zhang, X., Niu, M., Feng, N. 2014. Nanostructured lipid carriers versus microemulsions for delivery of the poorly water-soluble drug luteolin. International Journal of Pharmaceutics 476(1–2):169–177. DOI: 10.1016/j.ijpharm.2014.09.052.
  13. Luo, X., Zhou, Y., Bai, L., Liu, F., Deng, Y., McClements, D. J. 2017. Fabrication of β-carotene nanoemulsion-based delivery systems using dual-channel microfluidization: Physical and chemical stability. Journal of Colloid and Interface Science 490:328–335. DOI:10.1016/j.jcis.2016.11.057.
  14. Lv, W., Zhao, S., Yu, H., Li, N., Garamus, V. M., Chen, Y., Zou, A. 2016. Brucea javanica oil-loaded nanostructure lipid carriers (BJO NLCs): Preparation, characterization and in vitro evaluation. Colloids and Surfaces A: Physicochemical and Engineering Aspects 504:312–319. DOI:10.1016/j.colsurfa. 2016.05.068.
  15. McClements, D. J. 2010. Emulsion design to improve the delivery of functional lipophilic components. Annual Review of Food Science and Technology 1:241–269. DOI:10.1146/annurev.food.080708. 100722.
  16. McClements, D. J. 2017. The future of food colloids: Next-generation nanoparticle delivery systems. Current Opinion in Colloid and Interface Science 28:7–14. DOI:10.1016/j.cocis.2016.12.002.
  17. McClements, D. J., Rao, J. 2011. Food-grade nanoemulsions: formulation, fabrication, properties, performance, biological fate, and potential toxicity. Critical Reviews in Food Science and Nutrition 51(4):285–330. DOI:10.1080/10408398.2011.559558.
  18. Mohanraj, V. J., Chen, Y. 2007. Nanoparticles: A review. Tropical Journal of Pharmaceutical Research 5(1):561-573. DOI:10.4314/tjpr.v5i1.14634.
  19. Müller, R. H., Radtke, M., Wissing, S. A. 2002. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Advanced Drug Delivery Reviews 54:S131–S155. DOI:10.1016/S0169-409X(02) 00118-7.
  20. Okonogi, S., Riangjanapatee, P. 2015. Physicochemical characterization of lycopene-loaded nanostructured lipid carrier formulations for topical administration. International Journal of Pharmaceutics 478(2):726–735. DOI:10.1016/ j.ijpharm.2014.12.002.
  21. Pantzaris, T. P., Sue, T. T. 2017. Pocketbook of oil palm uses (Seventh Ed). Selangor, Malaysia: Malaysian Palm Oil Board (MPOB).
  22. Pardeike, J., Weber, S., Haber, T., Wagner, J., Zarfl, H. P., Plank, H., Zimmer, A. 2011. Development of an itraconazole-loaded nanostructured lipid carrier (NLC) formulation for pulmonary application. International Journal of Pharmaceutics 419(1–2):329–338. DOI:10.1016/j.ijpharm.2011.07.040.
  23. Prieto, C., Calvo, L. 2013. Performance of the biocompatible surfactant Tween 80, for the formation of microemulsions suitable for new pharmaceutical processing. Journal of Applied Chemistry:1–10. DOI:10.1155/2013/930356.
  24. Rowe, R. C. (Ed.). 2009. Handbook of pharmaceutical excipients (6. ed). London: APhA, (PhP) Pharmaceutical Press.
  25. Severino, P., Santana, M. H. A., Souto, E. B. 2012. Optimizing SLN and NLC by 22 full factorial design: effect of homogenization technique. Materials Science and Engineering: C 32(6):1375–1379. DOI:10.1016/j.msec.2012. 04.017.
  26. Shah, N. V., Seth, A. K., Balaraman, R., Aundhia, C. J., Maheshwari, R. A., Parmar, G. R. 2016. Nanostructured lipid carriers for oral bioavailability enhancement of raloxifene: Design and in vivo study. Journal of Advanced Research 7(3):423–434. DOI:10.1016/j.jare.2016.03.002.
  27. Tamjidi, F., Shahedi, M., Varshosaz, J., Nasirpour, A. 2013. Nanostructured lipid carriers (NLC): A potential delivery system for bioactive food molecules. Innovative Food Science and Emerging Technologies 19:29–43. DOI:10.1016/ j.ifset.2013.03.002.
  28. van Dijke, K., Kobayashi, I., Schroën, K., Uemura, K., Nakajima, M., Boom, R. 2010. Effect of viscosities of dispersed and continuous phases in microchannel oil-in-water emulsification. Microfluidics and Nanofluidics 9(1):77–85. DOI:10.1007/s10404-009-0521-7.
  29. Weiss, J., Decker, E. A., McClements, D. J., Kristbergsson, K., Helgason, T., Awad, T. 2008. Solid Lipid Nanoparticles as delivery systems for bioactive food components. Food Biophysics 3(2):146–154. DOI:10.1007/s11483-008-9065-8.
  30. Witayaudom, P., Klinkesorn, U. 2017. Effect of surfactant concentration and solidification temperature on the characteristics and stability of nanostructured lipid carrier (NLC) prepared from rambutan ( Nephelium lappaceum L.) kernel fat. Journal of Colloid and Interface Science, 505:1082–1092. DOI:10.1016/j.jcis.2017.07.008.
  31. Zheng, M., Falkeborg, M., Zheng, Y., Yang, T., Xu, X. 2013. Formulation and characterization of nanostructured lipid carriers containing a mixed lipids core. Colloids and Surfaces A: Physicochemical and Engineering Aspect 430:76–84. DOI:10.1016/j.colsurfa.2013.03.070.
  32. Zhu, J., Zhuang, P., Luan, L., Sun, Q., Cao, F. 2015. Preparation and characterization of novel nanocarriers containing krill oil for food application. Journal of Functional Foods 19:902–912. DOI:10.1016/j.jff.2015.06.017.
  33. Ziani, K., Fang, Y., McClements, D. J. 2012. Fabrication and stability of colloidal delivery systems for flavor oils: Effect of composition and storage conditions. Food Research International 1(46):209–216. DOI:10.1016/j.foodres.2011.12.017.