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Profil Bakteri Asam Laktat dan Evaluasi Sensori dari Tempe Bungkus Daun Jati yang Disuplementasi dengan Daun Kelor

*Stella Magdalena  -  Program Studi Teknologi Pangan, Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia
Yogiara Yogiara  -  Program Studi Magister Bioteknologi, Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia
Adi Yulandi  -  Program Studi Biologi, Fakultas Teknobiologi, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia

Citation Format:
Abstract

Tempe bungkus daun jati merupakan produk pangan fermentasi tradisional Indonesia yang kaya protein, serat, dan vitamin yang sangat popular di Kabupaten Blora, Jawa Tengah. Penelitian ini bertujuan mengetahui pengaruh penambahan daun kelor pada tahapan perendaman dan peragian dalam proses produksi tempe terhadap profil bakteri asam laktat (BAL) dan tingkat kesukaan. Daun kelor segar dan serbuk disuplementasikan dengan variasi konsentrasi pada proses produksi tempe kacang kedelai. Hasil penelitian menunjukkan bahwa daun kelor tidak menghambat aktivitas pertumbuhan dari kapang Rhizopus. Penambahan serbuk daun kelor sebanyak 1x dan 2x (b/b) dari total jumlah ragi pada proses peragian menghasilkan tekstur tempe yang kompak. Semakin tinggi konsentrasi air perasan daun kelor dalam proses perendaman, jumlah total bakteri asam laktat menurun hingga 103 CFU/g. Profil BAL dari tempe bungkus daun jati dengan ataupun tanpa suplementasi daun jati mengandung BAL dari genus Weissella. Tempe bungkus daun jati dengan suplementasi 1x (b/b) serbuk daun kelor sangat berpotensi untuk disosialisasikan sebagai pangan fungsional kepada masyarakat.

Lactic Acid Bacteria Profile and Sensory Evaluation of Teak Leaf-Wrapped Tempeh Supplemented with Moringa Leaves

Abstract

Tempeh wrapped in teak leaves is a traditional Indonesian fermented food product that is rich in protein, fiber, and vitamins which is very popular in Kabupaten Blora, Central Java. This study aimed to investigate the effect of Moringa leaves addition in the soaking and fermentation stages of tempeh production towards the profile of lactic acid bacteria (LAB) and the level of respondent acceptance. Fresh and powdered leaf was supplemented in various doses to soybean tempeh production. As results, Moringa leaves did not inhibit the growth of Rhizopus. The addition of Moringa leaf powder as much as 1x and 2x (w/w) of the total amount of yeast in the fermentation process produced a compact tempeh texture. The higher the concentration of Moringa leaf juice in the soaking process reduced total number of lactic acid bacteria to 103 CFU/g. LAB profile of teak leaf-wrapped tempeh with or without teak leaf powder supplementation contained LAB that belongs to the genus Weissella. Teak leaf-wrapped tempeh supplemented by 1x (w/w) Moringa leaf powder has a potential value to be socialized as functional food to the community.

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Keywords: tempe bungkus daun jati; daun kelor; bakteri asam laktat; sensori; probiotik; teak leaf-wrapped tempeh; Moringa leaves; lactic acid bacteria; sensory, probiotic
Funding: Pemerintah Kabupaten Blora

Article Metrics:

  1. Abalaka, M.E., Daniyan, S.Y., Oyeleke, S.B., Adeyemo, S.O. 2012. The antibacterial evaluation of Moringa oleifera leaf extracts on selected bacterial pathogens. Journal of Microbiology Research 2(2):1-4. DOI: 10.5923/j.microbiology.20120202.01
  2. Abubakr M.A.S., Al-Adiwih, W.M. 2017. Isolation and identification of lactic acid bacteria from different fruits with proteolytic activity. International Journal of Microbiology and Biotechnology 2(2):58-64. DOI: 10.11648/j.ijmb.20170202.12
  3. Akbar, C.T., Suketi, K., Kartika, J.G. 2019. Panen dan pascapanen kelor (Moringa oleifera Lam.) organik di Kebun Organik Kelorina, Blora, Jawa Tengah. Buletin Agrohorti 7(3):247-254. DOI: 10.29244/agrob.v7i3.30171
  4. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology 215:403-410. DOI: 10.1016/S0022-2836(05)80360-2
  5. Ambari, D.P., Anwar, F., Damayanthi, E. 2014. Formulasi sosis analog sumber protein berbasis tempe dan jamur tiram sebagai pangan fungsional kaya serat pangan. Jurnal Gizi dan Pangan 9(1):65-72. DOI: 10.25182/jgp.2014.9.1.%25p
  6. Arnanda, F., Kriswanto, Y., Izzatun, I., Nurlita, D., Fajriyani, A., Utami, T.W. 2015. Pemodelan ketahanan pangan kedelai (glysine soya max (Lenus&Merril) di Provinsi Jawa Tengah dengan pendekatan spatial regression. Jurnal Statistika 3(1):13-19. DOI: 10.26741/jsunimus.3.1.2015.%25p
  7. Astuti, M., Meliala, A., Dalais, F.S., Wahlqvist, M.L. 2001. Tempe, a nutritious and healthy food from Indonesia. Asia Pacific Journal of Clinical Nutrition 9(4):322-325. DOI: 10.1046/j.1440-6047.2000.00176.x
  8. Barus, T., Suwanto, A., Wahyudi, A.T., Wijaya, H. 2008. Role of bacteria in tempe bitter taste formastion: microbiological and molecular biological analysis based on 16S rRNA gene. Microbiology Indonesia 2(1):17-21. DOI: 10.5454/mi.2.1.4
  9. Bigot, C., Meile, J., Remize, F., Strub, C. 2016. Fermented foods. Part I, Biochemistry and Biotechnology. 1st edition, Montet D, Ray R-C, editor. CRC Press, Boca Raton, US. DOI: 10.1201/b19872
  10. Bourekoua, H., Rozylo, R., Gawlik-Dziki, U., Benatallah, L., Zidoune, M.N., Dziki, D. 2018. Evaluation of physical, sensorial, and antioxidant properties of gluten-free bread enriched with Moringa oleifera leaf powder. European Food Research and Technology 244:189-195. DOI: 10.1007/s00217-017-2942-y
  11. Chizoba, N.N. 2014. Sensory evaluation of cookies produced from different blends of wheat and Moringa oleifera leaf flour. International Journal of Nutrition and Food Sciences 3(4):307-310. DOI: 10.11648/j.ijnfs.20140304.21
  12. Diantoro, A., Rohman, M., Budiarti, R., Palupi, H.T. 2015. Pengaruh penambahan ekstrak daun kelor (Moringa oleifera L.) terhadap kualitas yoghurt. Jurnal Teknologi Pangan 6(2): 59-66. DOI: 10.35891/tp.v6i2.469
  13. Dinoto, A., Sulistiani, S., Handayani, R., Julistiono, H. 2018. Weissella paramesentroides from intestine of Indonesian eel (Anguilla bicolor McClelland) and their potential antimicrobial property. AIP Conference Proceedings. DOI: 10.1063/1.5062799
  14. Dixit, N.K.M., Hossain, S.K.A., Bharti, B.K., Singh, S.S., Mishra, S. 2018. Development of lassi using whey and moringa powder. International Journal of Current Microbiology and Applied Sciences 7(11):602-612. DOI: 10.20546/ijcmas.2018.711.073
  15. El-Sayed, O.F., El-Taweel, H.S.M., El-Shibiny, A.A., Metwally, M.M.K. 2016. Using moringa leaves powder in production of probiotic yoghurt. SINAI Journal of Applied Sciences 5(2):197-208. DOI: 10.21608/sinjas.2016.78645
  16. Fitriana, W.D., Ersam, T., Shimizu, K, Fatmawati, S. 2018. Antioxidant activity of Moringa oleifera extracts. Indonesian Journal of Chemistry 16(3):297–301. DOI: 10.22146/ijc.21145
  17. Fusco, V., Quero, G.M., Cho, G-S., Kabisch, J., Meske, D., Neve, H., Bockelmann, W., Franz, C.M.A.P. 2015. The genus Weissella: taxonomy, ecology, and biotechnological potential. Frontiers in Microbiology 6:155. DOI: 10.3389/fmicb.2015.00155
  18. Hogervorst, E., Sadjimim, T., Yesufu, A., Kreager, P., Rahardjo, T.B. 2008. High tofu intake is associated with worse memory in elderly Indonesian men and women. Dementia Geriatric and Cognitive Disorders 26(1):50-7. DOI: 10.1159/000141484
  19. Hogervorst, E., Mursjid, F., Priandini, D., Setyawan, H., Ismael, R.I., Stephan, B., Rahardjo, T.B. 2011. Borobudur revisited: Soy consumption may be associated with better recall in younger, but not in older, rural Indonesian elderly. Brain Research 1379:206–212. DOI: 10.1016/j.brainres.2010.10.083
  20. Ilona, A.D., Ismawati, R. 2015. Pengaruh penambahan ekstrak daun kelor (Moringa oleifera) dan waktu inkubasi terhadap sifat organoleptik yoghurt. Journal Tata Boga 4(3):151-159. DOI: 10.35891/tp.v6i2.469
  21. Imohiosen, O., Gurama, H.H., Lamidi, T.B. 2014. Phytochemical and antimicrobial studies on Moringa oleifera leaves extracts. IOSR Journal of Environmental Science, Toxicology, and Food Technology 8(1):39-45. DOI: 10.9790/2402-08143945
  22. Jans, C., Meile, L., Kaindi, D.W.M., Kogi-Makau, W., Lamuka, P., Renault, P., Kreikemeyer, B., Lacroix, C., Hattendorf, J., Zinsstag, J., Schelling, E., Fokou, G., Bonfoh, B. 2017. African fermented dairy products-overview of predominant technologically important microorganisms focusing on African Streptococcus infantairus variants and potential future applications for enhanced food safety and security. International Journal of Food Microbiology 250:27-36. DOI: 10.1016/j.ijfoodmicro.2017.03.012
  23. Kuikman, M., O’Connor, C.P. 2015. Sensory evaluation of Moringa-probiotic yoghurt containing banana, sweet potato, or avocado. Journal of Food Research 4(5):165-171. DOI: 10.5539/jfr.v4n5p165
  24. Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35(6): 1547-1549. DOI: 10.1093/molbev/msy096
  25. Lane, D.J. 1991. 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systemic. Stackebrandt E, Goodfellow M, editor, p 115-175. John Wiley, Chichester
  26. Laiṅo, J.E., Levit, R., de LeBlanc, A.M., de Giori, G.S., LeBlanc, J.G. 2019. Characterization of folate production and probiotic potential of Streptococcus gallolyticus subsp. macedonicus CRL415. Food Microbiology 79:20-26. DOI: 10.1016/j.fm.2018.10.015
  27. Lee, W.K., Ahn, S.B., Park, H.E., Lee, S.M., Kim, S.Y., Shon, M.Y. 2013. Characteristics and immuno-modulatory effects of Weissella cibaria JW 15 isolated from kimchi, Korea traditional fermented food, for probiotic use. Journal of Biomedical Research 14(4): 206-211. DOI: 10.12729/jbr.2013.14.4.206
  28. Liem, I.T., Steinkraus, K.H., Cronk, T.C. 1977. Production of vitamin B-12 in tempeh, a fermented soybean food. Applied and Environmental Microbiology 34(6):773–776. DOI: 10.1128/aem.34.6.773-776.1977
  29. Manenji, B.T., Mudyiwa, R.M., Midzi, J., Tsodzo, A. 2017. Antifungal effects of botanical leaf extracts of Lantana camara, Moringa oleifera, and Tagetes minuta on Rhizopus stolonifer in vitro. Journal of Agriculture and Ecology Research 11(2):1-8. DOI: 10.9734/JAERI/2017/28371
  30. Martins, E.M.F., Ramos, A.M., Vanzela, E.S.L., Stringheta, P.C., Pinto, C.L.O., Martins, J.M. 2013. Products of vegetable origin: a new alternative for the consumption of probiotic bacteria. Food Research International 51(2013):764.770. DOI: 10.1016/j.foodres.2013.01.047
  31. Moyo, B., Masika, P.J., Hugo, A., Muchenje. 2011. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology 10 (60):12925-12933. DOI: 10.5897/AJB10.1599
  32. Nout, M.J.R., Kiers, J.L. 2005. Tempe fermentation, innovation and functionality: update info the third millennium. Journal of Applied Microbiology 98:789-805. DOI: 10.1111/j.1365-2672.2004.02471.x
  33. Oluduro, A.O. 2012. Evaluation of antimicrobial properties and nutritional potentials of Moringa oleifera Lam. Leaf in South-Western Nigeria. Malaysian Journal of Microbiology 8(2):59-67. DOI: 10.21161/mjm.02912
  34. Radita, R., Suwanto, A., Kurosawa, N., Wahyudi, A.T., Rusmana, I. 2017. Metagenome analysis of tempeh production: where did the bacterial community in tempeh come from? Malaysian Journal of Microbiology 13(4): 280-288. DOI: 10.21161/mjm.101417
  35. Rambitan, G., Pelealu, J.J., Tallei, T.E. 2018. Isolasi dan identifikasi bakteri asam laktat hasil fermentasi kol merah (Brassica oleracea L) sebagai probiotik potensial. Jurnal Bios Logos 8(2): 33-37. DOI: 10.35799/jbl.8.2.2018.21447
  36. Razis, A.F.A., Ibrahim, M.D., Kntayya, S.B. 2014. Health benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention 15(20):8571-8576. DOI: 10.7314/APJCP.2014.15.20.8571
  37. Sarti, M.Y., Rhidowati, S., Lestari, S.D., Wulandari, R. 2019. Studi kesukaan panelis terhadap tempe dari biji lotus (Nelumbo nucifera) dan kedelai (Glycine max). Jurnal fishtech 8(2):34-41. DOI: 10.36706/fishtech.v8i2.9665
  38. Scourboutakus, M.J., Franco-Arellano, B., Murphy, S.A., Norsen, S., Comelli, E.M. Abbè, M.R.L. 2017. Mismatch between probiotic benefits in trial versus food products. Nutrients 9(4):400. DOI: 10.3390/nu9040400
  39. Sharma, S., Kandasamy, S., Kavitake, D., Shetty, P.H. 2018. Probiotic characterization and antioxidant properties of Weissella confusa KR780676, isolated from an Indian fermented food. LWT-Food Science and Technology 97(2018):53-60. DOI: 10.1016/j.lwt.2018.06.033
  40. Soka, S., Suwanto, A., Sajuthi, D., Rusmana, I. 2015. Impact of tempeh supplementation on mucosal immunoglobulin A in Sprague-Dawley rats. Food Science and Biotechnology 24(4):1481–1486. DOI: 10.1007/s10068-015-0191-z
  41. Stephanie, Kartawidjajaputra, F., Silo, W., Yogiara, Suwanto, A. 2018. Tempeh consumption enhanced beneficial bacteria in the human gut. Food Research 3(1):57-63. DOI: 10.26656/fr.2017.3(1).220
  42. Tripathi, M.K., Giri, S.K. 2014. Probotic functional foods: survival of probiotics during processing and storage. Journal of Functional Foods 9(2014):225-241. DOI: 10.1016/j.jff.2014.04.030
  43. Ulyatin, N.C., Ba’in. Eksploitasi hutan jati di Kabupaten Blora tahun 1845-1949. Journal of Indonesian History 9(1):10-16. DOI: 10.15294/jih.v9i1.40927
  44. Villarruel-López, A., López-de la Mora, D.A., Vázquez-Paulino, O.D., Puebla-Mora, A.G., Torres-Vitela, M.R., Guerrero-Quiroz, L.A., Nuño, K. 2018. Effect of Moringa oleifera consumption on diabetic rats. BMC Complementary and Alternative Medicine 18:127. DOI: 10.1186/s12906-018-2180-2
  45. Winarno, F.G., Reddy, N.R. 1986. Tempe. Di dalam: Reddy NR, editor. Legume based fermented foods. CRC Press, Boca Raton, US. DOI: 10.1201/9781351074001-13
  46. Yulianto, S. 2020. Identifikasi alkaloid pada daun kelor (Moringa oleifera L.). Jurnal Kebidanan dan Kesehatan Tradisional 5(1):55-57. DOI: 10.37341/jkkt.v5i1.136

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