JAFT • pISSN 2355-9152 • eISSN 2614-7076 • Member of CrossRef®
skip to main content

Sorbet Made from Moringa Leaves and Red Guava as an Alternative for the Management of Iron Deficiency Anemia in Adolescent Girls

*Tiffany Nisa Arviyani  -  Departement of Nutrition Science, Diponegoro University, Semarang, Indonesia
Diana Nur Afifah  -  Departement of Nutrition Science, Diponegoro University, Semarang, Indonesia
Etika Ratna Noer  -  Departement of Nutrition Science, Diponegoro University, Semarang, Indonesia
M. Zen Rahfiludin  -  Departement of Public Health, Diponegoro University, Semarang, Indonesia
Endang Mahati  -  Departement of Pharmacology and Therapy, Diponegoro University, Semarang, Indonesia
Open Access Copyright 2022 Journal of Applied Food Technology

Citation Format:
Abstract

Moringa leaves (Moringa oleifera) contain high iron (Fe) but low bioavailability of Fe because it contains calcium and tannins. Vitamin C in red guava fruit (Psidium guajava) can increase Fe absorption. Sorbet made from red guava and Moringa leaves can be an alternative to overcome iron deficiency anemia in adolescent girls. This study aims to analyze the level of acceptance, levels of Fe, Ca, vitamin C, and tannins, as well as the bioavailability of Fe sorbet made from red guava and Moringa leaves. Sorbet consists of 5 formulas including, F1 (strawberry sorbet), F2 (red guava sorbet), F3 (Moringa leaf sorbet), F4 (red guava sorbet + 18% Moringa leaves), and F5 (red guava sorbet + 22% Moringa leaves). Acceptance level was tested with the hedonic method (appearance, smell, taste, and texture) and statistically analyzed using Friedman and Wilcoxon test. The Fe and Ca were analyzed using X-Ray Fluorescence (XRF) method, vitamin C and tannins were analyzed using High-Performance Liquid Chromatography (HPLC) and spectrophotometry methods. Fe bioavailability test was performed using the inverted bowel bag method. The level of preference for each attribute was significantly different (p < 0.05). The level of preference chosen was F2. The mean levels of Fe, Ca, vitamin C, and tannins in F4 were 0.95 ± 0.14, 5.91 ± 0.73, 26.29 ± 0.13, and 135.65 ± 0.91 mg/100 g. The mean levels of Fe, Ca, vitamin C, and tannins in F5 were 0.21 ± 0.29, 6.03 ± 0.67, 46.72 ± 0.69, and 87.76 ± 0.40 mg/100 g. The highest Fe bioavailability was F5 while the lowest was F3. The best formula based on the parameters of preference, Fe, Ca, vitamin C, and tannin levels, as well as Fe bioavailability, was sorbet F5.

Fulltext View|Download
Keywords: sorbet; moringa leaf; red guava; adolescent girl; iron deficiency anemia
Funding: Faculty of Medicine, Diponegoro University, within the framework of a grant program with an Implementation Agreement No. 1213/UN7.5.4.2.1/PP/PM/2020.

Article Metrics:

  1. Afrianti L. 2013. Food preservation technology. Bandung: Alfabeta. (In Bahasa Indonesia)
  2. Almatsier S. 2009. Basic principles of nutrition. Jakarta: Gramedia. (In Bahasa Indonesia)
  3. Aminah, S., Ramdhan, T., & Yanis, M. 2015. Nutritional content and functional properties of moringa plants (Moringa oleifera). Buletin Pertanian Perkotaan 5(30): 35–44. (In Bahasa Indonesia)
  4. Badan Penelitian Kesehatan Kementrian Kesehatan RI. 2013. Basic Health Research 2013. (In Bahasa Indonesia)
  5. Badan Penelitian Kesehatan Kementrian Kesehatan RI. Basic Health Research 2018. (2018)
  6. Balarajan, Y., Ramakrishnan, U., Özaltin, E., Shankar, A. H., & Subramanian, S. V. (2011). Anaemia in low-income and middle-income countries. The Lancet, 378(9809), 2123–2135
  7. Bora, T., Aydin, H., Atac, Y., Sen, N., Aksoy, C. 2014. Determination of metals contamination in illicit ecstasy drug samples using ICP-OES and XRF. Atomic Spectroscopy 35(4):139-146. DOI: 10.46770/as/2014.04.001
  8. BPPHP., D. 2002. Guava. Jakarta: Departemen Pertanian Teknologi Pengolahan Hasil Hortikultura. (In Bahasa Indonesia)
  9. Cairo, R. C. de A., Silva, L. R., Bustani, N. C., & Marques, C. D. F. 2014. Anemia por deficiencia de hierro en adolescentes; una revision de la literatura. Nutricion Hospitalaria 29(6): 1240–1249
  10. Chandra, S., Kaushik, N., & Gupta, N. 2016. Study of Iron Status Indicators in Different Phases of Menstrual Cycle in Females of Lower Socio-Economic Group. Annals of International Medical and Dental research 3(1): 46-49. https://doi.org/10.21276/aimdr.2017.3.1.ph1
  11. Chen, J.M., Dehabadi, L., Ma. Y.C., Wilson, L.D. 2022. Development of novel lipid-based formulations for
  12. water-soluble vitamin C versus fat-soluble vitamin D3. Bioengineering 9(12). DOI: 10.3390/bioengineering9120819
  13. Direktrorat Gizi Masyarakat, K. R. 2018. Indonesian Food Composition Table 2017. Jakarta: Kementrian Kesehatan RI. (In Bahasa Indonesia)
  14. Damayanti, D.F., Astuti, W., Wati, E., Marsita, E. 2021. Effectiveness of honey and Fe tablets as an effort
  15. to increasing hemoglobin levels in adolescent women at islamic boarding school. Journal of Nutritionn Collage. 10(2):93-99. DOI: 10.14710/jnc.v10i2.29144
  16. Direktrorat Gizi Masyarakat, K.R. 2018. Indonesian Food Composition Table 2017. Jakarta: Kementrian Kesehatan RI. (In Bahasa Indonesia)
  17. Du Toit, E.S., Sithole, J., Vorster, J. 2020. Leaf harvesting severity affects total phenolic and tannin content of fresh and dry leaves of Moringa oleifera Lam. trees growing in Gauteng, South Africa. Soutch African Journal of Botany 129:336-340. DOI : 10.1016/j.sajb.2019.08.035
  18. Garcia, O. P., Diaz, M., Rosado, J. L., & Allen, L. H. (2003). Ascorbic acid from lime juice does not improve the iron status of iron-deficient women in rural Mexico. American Journal of Clinical Nutrition, 78(2), 267–273. https://doi.org/10.1093/ajcn/78.2.267
  19. Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness 5(2): 49–56
  20. Hardjanti, S. 2008. The potential of katuk leaves as a source of natural dyes and their stability during powder drying using a maltodextrin Binder. Jurnal Penelitian Saintek 13(1): 1–18. (In Bahasa Indonesia)
  21. Hassan, T. H., Badr, M. A., Karam, N. A., Zkaria, M., El Saadany, H. F., Rahman, D. M. A., Selim, A. M. 2016. Impact of iron deficiency anemia on the function of the immune system in children. Medicine (United States) 95(47): 1–5
  22. Ilona, A, D., & Ismawati, R. 2015. The Effect addition of Moringa leaf extract (Moringa oleifera) and incubation time on the organoleptic properties of yogurt. Jurnal Tata Boga 4(3): 151–159. (In Bahasa Indonesia)
  23. Iskandar AB, Ningtyias FW, R. N. 2019. Analysis of protein, calcium and acceptability of ice cream with the addition of moringa leaf flour (Moringa oleifera). Penelit Gizi Dan Makanan 42(2): 65–72. (In Bahasa Indonesia)
  24. Ismarani. 2012. The potential of tannin compounds in supporting environmentally friendly production. Jurnal Agribisnis Dan Pengembangan Wilayah 3(2): 46–55. (In Bahasa Indonesia)
  25. Khairiyah, S., Sinaga, S.M., Putra E.D.L. 2022. Determination of vitamin C and antioxidant activity in fresh red guava (Psidium Guajava L.) and red guava commercial fruit juices. International Journal of Science, Technology & Management 3(4):880-883. DOI : 10.46729/ijstm.v3i4.543
  26. Khoja, K. K., Buckley, A., Aslam, M. F., Sharp, P. A., & Latunde-dada, G. O. 2020. In Vitro Bioaccessibility and Bioavailability of Iron from Mature and Microgreen Fenugreek, Rocket and Broccoli. In Vitro Bioaccessibility and Bioavailability of Iron from Mature and Microgreen Fenugreek, Rocket and Broccoli
  27. Kounnavong, S., Vonglokham, M., Kounnavong, T., Kwadwo, D. D., & Essink, D. R. 2020. Anaemia among adolescents: assessing a public health concern in Lao PDR. Global Health Action, 13(sup2)
  28. Mahattanatawee, K., Manthey, J. A., Luzio, G., Talcott, S. T., Goodner, K., & Baldwin, E. A. 2006. Total antioxidant activity and fiber content of select Florida-grown tropical fruits. Journal of Agricultural and Food Chemistry 54(19): 7355–7363
  29. Miftahur, R., Rita, I. 2014. Effect of carrageenan amount and extract of pandan wangi leaves (Pandanus amaryllifolius) on the organoloptic properties of jelly drink moringa leaves (Moringa oleifera). E-Journal Boga 03: 1–11. (In Bahasa Indonesia)
  30. Muchlisun, A., Praptiningsih, Y., Choiron, M. 2015. Characteristics of dipped manalagi apples made with variations in blanching time and drying temperature. Berkala Ilmiah Pertanian, 10(1), 97–112. (In Bahasa Indonesia)
  31. Nurhaini, R., Annisa, M.A., Mustofa, C.H. 2018. Analysis of iron (Fe) content in moringa leaves. Pharmacy
  32. Science 9(1): 17-25. (In Bahasa Indonesia)
  33. Olagbemide, P.T., Alikwe, P.C.N. 2015. Proximate Analysis and Chemical Composition of Raw and Defatted Moringa oleifera Kernel Ecotoxicological Assessment of fish in aquatic environment View
  34. project Project View project. Advances in Life Science and Technology 24: 92–100
  35. Rini, A. K., & Ishartani, D. (2012). The effect of the combination of CMC stabilizer and arabic gum on the quality of carrot velva (Daucus carota L.) Selo and Tawangmangu varieties. Jurnal Teknosains Pangan 1(1): 86–94. (In Bahasa Indonesia)
  36. Rosyidah, A. Z., 2016. A study on the level of respondents' preference for the diversification of side dishes from Moringa (Moringa oleivera) leaves. e-Journal Boga 5(1): 17–22. (In Bahasa Indonesia)
  37. Saini, R. K., Manoj, P., Shetty, N. P., Srinivasan, K., Giridhar, P. 2014. Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats. Nutrition Research 34(7): 630–638
  38. Saunders, A. V., Craig, W. J., Baines, S. K., Posen, J. S. 2012. Iron and vegetarian diets. Medical Journal of Australia, 1(June), 11–16. https://doi.org/10.5694/mjao11.11494
  39. Shekhawat, S., P P, G., Gupta, M., Kakkar, M., Mathur, M., Ahaluwalia, N., Pahal, P. 2014. A study of nutritional and health status of adolescent girls (10 - 19 Years) in Jaipur City. Journal of Evolution of Medical and Dental Sciences 3(16): 4299–4309
  40. Soliman, A., De Sanctis, V., Kalra, S. 2014. Anemia and growth. Indian Journal of Endocrinology and Metabolism 18, S1–S5. https://doi.org/10.4103/2230-8210.145038
  41. Sudiarta, N.P. 2022. Utiliation of moringa leaf powder as a jelly candy flavvor. The journal gastronomy
  42. tourism 9(2):99-107. DOI: 10.17509/gastur.v9i52378
  43. Taghipoor, M., Barles, G., Georgelin, C., Licois, J. R., Lescoat, P. 2014. Digestion modeling in the small intestine: Impact of dietary fiber. Mathematical Biosciences 258: 101–112
  44. Tessera, G. M., Haile, A., Kinfe, E. 2015. Bioavailability of Minerals in Cookies Developed from Blend of Moringa Leaf Powder and Wheat Flour for Iron Deficient Lactating Mothers. International Journal of Food Science and Nutrition Engineering 5(6): 226–232
  45. Winarno F. G. 2006. Food Chemistry and Nutrition. Jakarta: PT Gramedia Pustaka Utama. (In Bahasa Indonesia)
  46. Yang, R., Chang, L., Hsu, C., Weng, B., Palada, M., Chadha, M., Levasseur, V. 2006. Nutritional and functional properties of Moringa Leaves from germplasm, to plant, to food, to health. In: Moringa and Other Highly Nutritious Plant Resources: Strategies, Standards and Markets for a Better Impact on Nutrition in Africa. American Chemical Society: 1–9
  47. Yang, R. Y., Tsou, S. C. S. 2006. Enhancing iron bioavailability of vegetables through proper preparation principles and applications. AVRDC-The World Vegetable Center, Taiwan. J International Cooperation 1(June 2006): 107–119

Last update:

No citation recorded.

Last update:

No citation recorded.