Ekstrak Angkak dan Bekatul untuk Mencegah Peroksidasi Lipid Tikus Spague-Dawley Hiperglikemik

*Hasim Hasim scopus  -  Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor, Indonesia
Moh Asroriy Nuris  -  Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor, Indonesia
Agus Setyono  -  Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor, Indonesia
Eka Nurul Qomaliyah  -  Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor, Indonesia
Didah Nur Faridah  -  Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor, Indonesia
Received: 10 Oct 2019; Revised: 22 Apr 2020; Accepted: 25 Apr 2020; Published: 10 May 2020; Available online: 10 May 2020.
DOI: https://doi.org/10.17728/jatp.6077 View
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Open Access License URL: http://creativecommons.org/licenses/by-nc/4.0

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Abstract

Angkak dan bekatul merupakan pangan fungsional yang berpotensi sebagai agen terapi untuk mengendalikan gula darah, dikarenakan kandungan antioksidan alaminya. Tujuan penelitian adalah untuk mengevaluasi pengaruh campuran ekstrak angkak dan bekatul dalam menghambat peroksidasi lipid serta efeknya terhadap organ pankreas, hati dan ginjal tikus hiperglikemik secara histopatologi. Tikus dibagi ke dalam 7 kelompok yaitu kelompok kontrol normal, kontrol negatif, kontrol positif, formulasi ekstrak angkak bekatul kelompok I, II, III dan IV (20:1400, 40:700, 30:1050, dan 60:2100 mg/kg BB). Parameter pengujian yang dilakukan adalah konsentrasi malondialdehid (MDA) dan aktivitas enzim aminotransferase (ALT/AST) serum darah tikus dan histopatologi organ pankreas, hati dan ginjal. Campuran terbaik dalam menghambat pembentukan MDA dan peningkatan enzim ALT/AST adalah kelompok III dengan konsentrasi MDA (3,.31±0,.53 nmol/ml) dan aktivitas enzim ALT (35,.80±5,.63 U/l) tidak berbeda nyata dengan kontrol normal (4,.85±2,.32 nmol/ml) dan (27,.85±8,.13), sementara aktivitas enzim AST (67,.221±16,.05) mengalami penurunan namun berbeda nyata dengan kontrol normal (4,.365±0,.00). Kondisi histopatologi organ pankreas, hati, dan ginjal tikus semua kelompok perlakuan menunjukkan kemampuan dalam memperbaiki kerusakan organ. Kesimpulannya, campuran angkak dan bekatul 30:1050 mg/kg BB memiliki kemampuan terbaik dalam meghambat peroksidan lipid.

Angkak and Rice Bran Extract to Prevent Lipid Peroxidation in Spague-Dawley Rats Hyperglycemic

Abstract

Angkak and rice bran is a functional food that has potential as a therapeutic agent to control blood sugar. This potential is due to the content of natural antioxidants in red yeast rice and rice bran. The objective of this research was to evaluate the effect of angkak and rice bran extract as lipid peroxidation inhibition. The effect on the pancreatic, liver and kidney organs in hyperglycemic rats by histopatological study was also analyzed. Rats were divided into 7 groups namely normal control group, negative control, positive control, rice bran extract formulation on gorup I, II, III, IV (20: 1400, 40: 700, 30: 1050, and 60: 2100 mg/kg BW). The analysis parameters carried out were the concentration of malondialdehyde (MDA), the activity of the serum alanine/aspartat aminotransferase (ALT/AST) enzymes and rat histopathology of pancreatic, liver and kidney organs. The best mixture of angkak and rice bran to inhibit the formation of MDA and increase the ALT/AST enzyme was group III with MDA concentration (3,.31±0.,53 nmol/ml) and ALT enzyme activity (35.,80±5,.63 U/l) was not significantly different from normal control (4,.85±2,.32 nmol/ml) and (27.,85±8,.13), while the enzyme activity of AST (67,221±16,.05) decreased but was significantly different from normal controls (4,365±0,.00). Histopathological conditions of pancreatic, liver, and kidney organs of rats in all treatment groups showed the ability to repair damaged organs. In conclusion, the mixture of red yeast rice and rice bran 30:1050 mg/kg BW had the best ability to inhibit lipid antioxidants.

Note: This article has supplementary file(s).

Keywords: Aminotransferase; Histopathology; Hyperglycemia; Malondialdehyde; Lipid Peroxidation; aminotransferase; histopatologi; hiperglikemia; malondialdehid; peroksidasi lipid

Article Metrics:

  1. IFCC [International Federation of Clinical Chemistry]. 1986. Methods for the measurement of catalytic concentration of enzymes. Journal of Clinical Chemistry and Clinical Biochemistry 24:481. PMID: 3734711.
  2. Aalim, H., Belwal, T., Wang, Y., Luo, Z., Hu, J. 2018. Purification and identification of rice bran (Oryza sativa L .) phenolic compounds with in-vitro antioxidant and antidiabetic activity using macroporous resins. International Journal of Food Science and Technology 54(3): 715-722. DOI: 10.1111/ijfs.13985
  3. Akihisa, T., Tokuda, H., Yasukawa, K., Ukiya, M., Kiyota, A., Sakamoto, N., Suzuki, T., Tanabe, N., Nishino, H. 2005. Azaphilonces, furanoisophthalides, and amino acids from the extracts of monascus pilosus-fermented rice (red-mold rice) and their chemopreventive effects. Journal of Agricultural and Food Chemistry 53(3): 562–565. DOI: 10.1021/jf040199p
  4. Antula Y., Neno D.S., Arie, S., Chanif, M. 2019. Dietary rice bran plays a significant role in the hepatoprotective effect in hypercholesterolemic rats. The Journal of Experimental Life Science. 9(2): 110-115.
  5. Ashcroft, F., Rorsman P. 2012. Leading edge review: diabetes mellitus and the β Cell: the last ten years. Cell 148(6): 1160-1171. DOI: 10.1016/j.cell.2012.02.010
  6. Baskar, A. A.,. Khalid, A., Gabriel, P., Mohammed, A., May, A., Ignacimuthu S. 2012. β sitosterol prevents lipid peroxidation and improves antioxidant status and histoarchitecture in rats with 1,2-Dimethylhydrazine induced colon cancer. Journal of Medicinal Food 15(4):335–343. DOI: 10.1089/jmf.2011.1780
  7. Boonloh, K., Kukongviriyapan, U., Pannangpetch, P., Kongyingyoes, B., Senggunprai, L., Prawan, A., Thawornchinsombut, S., Kukongviriyapan, V. 2015. Rice bran protein hydrolysates prevented interleukin-6- and high glucose-induced insulin resistance in hepg2 cells. Food Function 6(2):566–573. DOI: 10.1039/c4fo00872c
  8. Boue, S., Daigle, K.W., Ming-Hsuan, C., Heping, C., Mark, H.L. 2016. Antidiabetic potential of purple and red rice (Oryza sativa L.) bran extracts. Journal of Agricultural and Food Chemistry 64(26):5345−5353. DOI: 10.1021/acs.jafc.6b01909
  9. Brouns, F. 2018. Overweight and diabetes prevention: is a low-carbohydrate–high-fat diet recom mendable. European Journal of Nutrition 57:1301–1312. DOI: 10.1007/s00394-018-1636-y
  10. Catala, A. 2010. A synopsis of the process of lipid peroxidation since the discovery of the essential fatty acids. Biochemical and Biophysical Research Communications 399 (1): 318–323. DOI: 10.1016/j.bbrc.2010.07.087
  11. Chen, C.C., Liu I., M. 2006. Release of acetylcholine by Hon-Chi to raise insulin secretion in wistar rats. Neuroscience Letter 404(1-2):117–121. DOI: 10.1016/j.neulet.2006.05.024
  12. Cui, Y., Xingbin, Y., Xinshan L., Jinwen, C., Yan, Z. 2014. Protective effects of polyphenols-enriched extract from Huangshan Maofeng green tea against CCl4-induced liver injury in mice. Chemico-Biological Interactions 220(1):75-83. DOI: 10.1016/j.cbi.2014.06.018
  13. Dahech I., Belghith, K.S., Hamden, K., Feki, A., Belghith, H., Mejdouba, H. 2011. Oral administration of levan polysaccharide reduces the alloxan-induced oxidative stress in rats. International Journal of Biological Macromolecules 49 (5):942– 947. DOI: 10.1016/j.ijbiomac.2011.08.011
  14. Damayanthi, E., Kustiyah, L., Khalid, M., Farizal, H. 2010. Aktivitas antioksidan bekatul lebih tinggi dari pada jus tomat dan penurunan aktivitas antioksidan serum setelah intervensi minuman kaya antioksidan. Jurnal Gizi Pangan 5(3):205-210. DOI: 10.25182/jgp.2010.5.3.205-210
  15. Dwivedi, D.K., Jena, G.B. 2018. Glibenclamide protects against thioacetamideinduced hepatic damage in wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation. Naunyn-Schmiedeberg's Archives of Pharmacology 391(11): 1257-1274. DOI: 10.1007/s00210-018-1540-2
  16. Esterbauer, H., Eckl, P., Ortner, A. 1990. Possible mutagens derived from lipids and lipid precursors. Mutation Research/Reviews in Genetic Toxicology 238(3):223–33. DOI: 10.1016/0165-1110(90)90014-3
  17. Esterbauer, H., Schauer, R., Zollner, H. 1991. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology and Medicine 11(1):81–128. DOI: 10.1016/0891-5849(91)90192-6
  18. Garcia, C.A., Gavino, G., Mosqueda, M., Hevia, P., Gavino, V.C. 2007. Correlation of tocopherol, tocotrienol, γ-oryzanol and total polyphenol content in rice bran with different antioxidant capacity assays. Food Chemistry 102(4):1228-1232. DOI: 10.1016/j.foodchem.2006.07.012
  19. Giera, M., Lingeman, H., Niessen, W., M., A. 2012. Recent advancements in the LC- and GC-based analysis of malondialdehyde (MDA): a brief overview. Chromatographia 75(9–10):433–40. DOI: 10.1007/s10337-012-2237-1
  20. Giknis, M., Clifford, C., B. 2008. Clinical Laboratory Parameters for Crl, WI (Han). Charles River, Wilmington, US.
  21. Gordon, R., Y., Cooperman, T., Obermeyer, W., Becker, D.J. 2010. Marked variability of monacolin levels in commercial red yeast rice products. Archive of Internal Medicine 170(19):1722-1727. DOI: 10.1001/archinternmed.2010.382
  22. Govindarajan S, Vellingiri K. 2016. Effect of red yeast rice and coconut, rice bran or sunflower oil in rats on hypercholesterolemic diet. Journal of Clinical and Diagnostic Research 10(4). DOI: 10.7860/JCDR/2016/18623.7624
  23. Grotto, D., Santa M.,L., Valentini, J., Paniz, C., Schimtt, G., Garcia, S.,C., Pomblum, V.,J., Rocha, J.,B., T., Farina, M. 2009. Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantifi cation. Química Nova 32(1):169 – 174. DOI: 10.1590/S0100-40422009000100032
  24. Halliwell, B., Gutteridge, J.,M.,C. 1999. Lipid peroxidation: a radical chain reaction. In: Halliwell, B., Gutteridge, J.M.C. (Eds.), Free radicals in biology and medicine. Page 188–276. Oxford, UK: Clarendon Press.
  25. Handani, A.R., Salim, M.N., Harris, A., Budiman, H., Zainuddin, Sugito. 2015. Pengaruh pemberian kacang panjang (Vigna unguiculata) terhadap struktur mikroskopis ginjal mencit (Mus musculus) yang diinduksi aloksan. Jurnal Medika Veterineria 9(1):18-22. DOI: 10.21157/j.med.vet..v9i1.2987.g2821
  26. Jaeschke, H. 2010. The mechanism of cell antioxidant defense. In: Roth RA, Ganey P Comprehensive Toxicology, Volume IX: Hepatic Toxicology. Oxford, England: Elsevier.
  27. Jung, C.H., Lee, D.H., Ahn, J., Lee, H., Choi, W.H., Jang, Y.J. 2015.γ-Oryzanol enhances adipocyte differentiation and glucose uptake. Nutrients 7(6):4851-4861. DOI: 10.3390/nu7064851
  28. Justo, M.L., Rodriguez-Rodriguez, R., Claro, C. M., Alvarez, D.M., Parrado, J., Herrera, M.D. 2013. Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese zucker rats. European Journal of Nutrition 52(2): 789–797. DOI: 10.1007/s00394-012-0385-6
  29. Kasim, E., Suharna, N., Nurhidayat, N. 2006. Pigment and lovastatin content on the red rice cultivar bah butong and BP 1804 IF 9 which fermented by Monascus purpureus Jmba. Biodiversitas 7(1): 7-9. DOI: 10.13057/biodiv/d070103
  30. Kaup, R.M., Khayyal, M.T., Verspohl, E.J. 2013. Antidiabetic effects of a standardized egyptian rice bran extract. Phytotheraphy Research 27(2): 264–271. DOI: 10.1002/ptr.4705
  31. Kong, F., Su, Z., Guo, X., Zeng, F., Bi, Y. 2017. Antidiabetic and lipid-lowering effects of the polyphenol extracts from the leaves of clausena lansium (Lour.) skeels on streptozotocin-induced type 2 diabetic rats. Journal of Food Science 83(1): 212–220. doi: 10.1111/1750-3841.14004
  32. Koolman, J., Roehm, K.H. 2012. Color Atlas of Biochemistry. Thieme, New York (US).
  33. Li, H., Xie Y.H., Yang, Q., Wang S.W., Zhang B.L., Wang J.B., Cao, W., Lin-L.B., Sun, J.Y., Miao, S., Hu, J., Zhou, X., Qiu, P. 2012. Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats. PLoS ONE 7(11): e48872. DOI: 10.1371/journal.pone.0048872
  34. Lykkesfeldt, J. 2007. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clinica Chemica Acta 380(1):50–58. DOI: 10.1016/j.cca.2007.01.028
  35. Mahendran, S., Badami, S., Maithili, V. 2011. Evaluation of antidiabetic effect of embelin from Embelia ribes in alloxan induced diabetes in rats. Biomedicine and Preventive Nutrition 1(1):25-31. DOI: 10.1016/j.bionut.2010.08.002
  36. Mappa, I., S, Kairupan, C., Loho, L. 2013. Gambaran histologi ginjal tikus putih (wistar) setelah pemberian rifampisin. Journal e Biomedik 1(1):338-342. DOI: 10.35790/ebm.1.1.2013.4368
  37. Masjedia F, Golb A, Dabiric S. 2013. Preventive effect of garlic (Allium sativum L.) on serum biochemical factors and histopathology of pancreas and liver in streptozotocinInduced diabetic rats. Iranian Journal of Pharmaceutical Research 12 (3): 325-338.
  38. Maritim, A. C., Sanders, R.A., Watkins, J.B. 2003. Diabetes, oxidative stress, and antioxidants: a review. Journal Biochemical and Molecular Toxicology 17 (1): 24–38. DOI: 10.1002/jbt.10058
  39. Matough, F.A., Budin, S.B., Hamid, Z.A., Alwahaibi, N., Mohamed, J. 2012. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos University Medical Journal 12(1)5–18. DOI: 10.12816/0003082
  40. Pasaoglu, H., Sancak, B., Bukan, N. 2004. Lipid peroxidation and resistance to oxidation in patients with type 2 diabetes mellitus. Tohuku Journal Experimental Medicine 203(3): 211–218. DOI: 10.1620/tjem.203.211
  41. Pathel, D.K., Kumar, R., Laloo, D., Hemalatha, S. 2012. Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity. Asian Pacific Journal of Tropical Biomedicine 2(5):411–20. DOI: 10.1016/S2221-1691(12)60067-7
  42. Posuwan, J., Prangthip, P., Leardkamolkarn, V., Yamborisut, U., Surasiang, R., Charoensiri, R., Kongkachuichai, R. 2013. Long-term supplementation of high pigmented rice bran oil (Oryza sativa L.) on amelioration of oxidative stress and histological changes in streptozotocin-induced diabetic rats fed a high fat diet; riceberry bran oil. Food Chemistry 138(1):501–508. DOI: 10.1016/j.foodchem.2012.09.144
  43. Rajasekran A, Kalaivani M, Sabitha R. 2009. Anti-diabetic activity of aqueous extract of monascus purpureus fermented rice in high cholesterol diet fed-streptozotocininduced diabetic rats. Asian Journal of Scientific Research 2(4):180-189. DOI: 10.3923/ajsr.2009.180.189
  44. Sebai, H., Selmi, S., Rtibi, K., Gharbi, Najoua., Sakly, M. 2014. Protective effect of Lavandula stoechas and Rosmarinus officinalis essential oils against reproductive damage and oxidative stress in alloxan-induced diabetic rats. Journal of Medicinal Food 0(0): 1-9. DOI: 10.1089/jmf.2014.0040
  45. Shi, Y.C, Pan, T.M. 2010. Anti-diabetic effects of Monascus purpureus NTU 568 fermented products on streptozotocin-induced diabetic rats. Journal of Agricultural and Food Chemistry 58(13):7634-7640. DOI: 10.1021/jf101194f
  46. Subashbabu, P., Alshatwi, A., Ignacimuthu, S. 2014. Beneficial antioxidative and antiperoxidative effect of cinnamaldehyde protect streptozotocin-induced pancreatic b-cells damage in wistar Rats. Biomolecules and Therapeutic 22(1):47-54. DOI: 10.4062/biomolther.2013.100
  47. Tiwari AK, Swapna M, Ayesha SB, Zehra A, Agawane SB, Madhusudana K. 2011. Identification of proglycemic and antihyperglycemic activity in antioxidant rich fraction of some common food grains. International Food Research Journal 18(3):915-923. DOI: 10.1111/j.1745-4514.2011.00547.x
  48. Wahyuningsih, D., Elyani, H., Sri D.D., Yahya, A., Fadli, M.Z. 2019. Red yeast rice protects hepatocytes conditions of rats receiving high fat diet. Journal of Tropical Life Science 9(2): 171 – 178. DOI: 10.11594/jtls.09.02.06
  49. Washington, I.M., Hoosier, V.G. 2012. Clinical biochemistry and hematology. Di dalam: The Laboratory Rabbit, Guinea Pig, hamster, and Other Rodents. Suckow MA, Stevens KA, Wilson RP, editor. Academic Pr, London.
  50. Xu, Z., Godber, J.S. 1999. Purification and identification of components of oryzanol in rice bran oil. Journal of Agricultural and Food Chemistry 47:2724-2728. DOI: 10.1021/jf981175j
  51. Yagi, T. 1987. Differential sensitivities of transformed and untransformed murine cell lines to DNA cross-linking agents relative to repair of O6-methylguanine. Mutation Research 184:223–7. DOI: 10.1016/0167-8817(87)90020-4