Jurnal pangan nasional "terakreditasi" Kemeristekdikti dari Indonesian Food Technologists® - IFT
skip to main content

Studi Karakteristik dan Uji Aktivitas Antioksidan dari Tepung Buah Okra (Abelmoschus esculentus)

Ahdiyatul Fauza  -  Magister Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
Kis Djamiatun  -  Departemen Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
*Ahmad Ni'matullah Al-Baarri  -  Program Studi Teknologi Pangan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, Indonesia

Citation Format:
Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan saat sebelum dan setelah pengeringan buah Okra. Analisis fisik meliputi analisis warna dan PSD (Particle Size Distribution). Analisis kimia meliputi uji pH, conductivity dan TDS (Total Disolve Solid). Uji aktivitas antioksidan dilakukan dengan metode DPPH. Analisis data warna menggunakan uji korelasi. Data PSD, uji pH, konduktivitas, TDS, dan aktivitas antioksidan disajikan secara deskriptif. Hasil penelitian menunjukkan bahwa pengeringan buah Okra menjadi tepung Okra, tidak merusak aktivitas antioksidan. Proses pengeringan tepung Okra meningkatkan derajat kecerahan Okra dengan hasil uji statistik nilai L* <0,0001 dan a* 0,0002 (p<0,05). Distribusi ukuran partikel tepung Okra lebih kecil sehingga mudah larut dalam air. Buah Okra memiliki pH yang lebih asam yang mengakibatkan konduktivitasnya menjadi lebih tinggi sehingga padatan yang terlarut (kation, anion, mineral) juga semakin tinggi. Kesimpulannya, aktivitas antioksidan tepung buah Okra dapat diidentifikasi baik sebelum maupun setelah pengeringan dan proses pengeringan dapat berhasil memelihara aktivitas antioksidannya.

This study aims to determine the physical, chemical, and antioxidant activities before and after drying the Okra fruit. Color analysis and PSD (Particle Size Distribution) were measured for physical analysis. pH test, conductivity, and TDS (Total Disolve Solid) was also measured as chemical analysis. Antioxidant activity was tested using DPPH. Color data was analyzed using correlation test. PSD data, pH test, conductivity, TDS, and antioxidant activity were presented descriptively. The results showed that drying Okra fruit to produce Okra flour did not damage antioxidant activity. The drying process of Okra flour brightening the color of the Okra fruit and in accordance with the statistical test that was resulted value of L* <0.0001 and a* 0,0002 (p <0.05). The particle size distribution of Okra flour was categorized as small. A much more acidic pH and higher in conductivity were found in the fresh Okra. As conclusion, antioxidant activity of Okra flour could be determined and the drying may be applied since able to maintain antioxidant activity in Okra flour.

Fulltext View|Download
Keywords: Okra; aktivitas antioksidan; pengeringan; oven; uji karakteristik; Okra; antioxidant activity; drying; oven; characteristic analysis, ocre

Article Metrics:

  1. Al-Baarri, A.N., Legowo, A.M., Pramono, Y.B., Rizqiati, H., Pratama, Y., Masykuri, Widayat. 2019. Stability analysis of ginger (Zingiber officinale) emulsion affected by iota carrageenan. IOP Conference Series: Earth and Environmental Science 309:012038. DOI: 10.1088/1755-1315/309/1/012038
  2. Alpizar-Reyes, E., Carrillo-Navas, H., Gallardo-Rivera, R., Varela-Guerrero, V., Alvarez-Ramirez, J., Pérez-Alonso, C. 2017. Functional properties and physicochemical characteristics of tamarind (Tamarindus indica L.) seed mucilage powder as a novel hydrocolloid. Journal of Food Engineering (209):68–75. DOI: 10.1016/j.jfoodeng.2017.04.021
  3. Adelakun O.E., Oyelade, O.J., Ade-omowaye, B.I.O., Adeyemi, I.A., Van De, V.M. 2009. Chemical composition and the antioxidative properties of Nigerian Okra Seed (Abelmoschus esculentus Moench) Flour. Food and Chemical Toxicology 47(6): 1123–1126. DOI: 10.1016/j.fct.2009.01.036
  4. Arapitsas, P. 2008. Identification and quantification of polyphenolic compounds from Okra seeds and skins. Food Chemical 110(4): 1041–1045. DOI: 10.1016/j.foodchem.2008.03.014
  5. Bernhardt, S., Schlich, E. 2006. Impact of different cooking methods on food quality : Retention of lipophilic vitamins in fresh and frozen vegetables. Journal Food Engineering 77(2): 327–333. DOI: 10.1016/j.jfoodeng.2005.06.040
  6. Carpin, M., Bertelsen, H., Dalberg, A., Bech, J.K., Risbo, J., Schuck, P., Jeantet, R. 2017. How does particle size in fluence caking in lactose powder. Journal of Food Engineering 209: 61–67. DOI: 10.1016/j.jfoodeng.2017.04.006
  7. Chumyam, A., Whangchai, K., Jungklang, J., Faiyue, B. 2013. Effects of heat treatments on antioxidant capacity and total phenolic content of four cultivars of purple skin eggplants. Science Asia 39: 246–251. DOI: 10.2306/scienceasia1513-1874.2013.39.246
  8. Coskun, O., Kanter, M., Korkmaz, A., Oter, S. 2005. Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Pharmacological Research 51:117–123. DOI: 10.1016/j.phrs.2004.06.002
  9. Dadali, G., Apar D.K., Ozbek, B. 2017. Color change kinetics of Okra undergoing microwave drying color change kinetics of Okra undergoing microwave drying. Drying Technology 3937:925–936. DOI: 10.1080/07373930701372296
  10. Edwards, R.L., Lyon, T., Litwin, S.E., Rabovsky, A., Symons, J.D. 2018. Quercetin reduces blood pressure in hypertensive subjects. The Journal of Nutrition 137(11):2405–2411. DOI: 10.1093/jn/137.11.2405
  11. El, S.N., Karakaya, S. 2004. Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet. International Journal Food Science Nutrition 55:67–74. DOI: 10.1080/09637480310001642501
  12. Fan S., Zhang, Y., Sun, Q., Yu, L., Li, M., Zheng, B., Wu, X., Yang, B., Li, Y., Huang, C. 2014. Extract of Okra lowers blood glucose and serum lipids in high-fat dietinduced obese C57BL/6 mice. Journal Nutrition Biochemical 25(7):702–709. DOI: 10.1016/j.jnutbio.2014.02.010
  13. Filler, K., Lyon, D., Bennett, J., McCain, N., Elswick, R., Lukkahatai, N., Saligan, L.N. 2014. Association of mitochondrial dysfunction and fatigue: a review of the literature. BBA Clinical (1):12–23. DOI: 10.1016/j.bbacli.2014.04.001
  14. Giacco, F., Brownlee, M. 2014. Oxidative stress and diabetic complications. The American Heart Association: 1058–1070. DOI: 10.1161/CIRCRESAHA.110.223545
  15. Imanningsih, N. 2012. Profil gelatinisasi beberapa formulasi tepung-tepungan untuk pendugaan sifat pemasakan. Penelitian Gizi dan Makanan 35(1):13–22
  16. Juin, F., Shan, Y., June, M., Dee, W. 2012. Effects of Chinese domestic cooking methods on the carotenoid composition of vegetables in Taiwan. LWT - Food Science and Technology 46(2):485–492. DOI: 10.1016/j.lwt.2011.11.019
  17. Kedare, S.B., Singh, R.P. 2011. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology 48(4):412–422. DOI: 10.1007/s13197-011-0251-1
  18. Krokida, M.K., Marinos, K. 2003. Drying kinetics of some vegetables. Journal of Food Engineering 59:391–403. DOI: 10.1016/S0260-8774(02)00498-3
  19. Lin, Y., Liu, H., Fang, J., Yu, C., Xiong, Y., Yuan, K. 2014. Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats. Food and Chemical Toxicology 68: 290–296. DOI: 10.1016/j.fct.2014.03.026
  20. Lombard, K., Peffley, E., Geoffriau, E., Thompson, L., Herring, A. 2005. Quercetin in onion (Allium cepa L .) after heat-treatment simulating home preparation. Journal of Food Composition and Analysis 18:571–581. DOI: 10.1016/j.jfca.2004.03.027
  21. Mendoza, F., Petr, D.J.M., Aguilera. 2007. Colour and texture analysis in classification of commercial potato chips. Food Research International 40:1146–1154. DOI: 10.1016/j.foodres.2007.06.014
  22. Miglio, C., Chiavaro, E., Visconti, A., Fogliano, V., Pellegrini, N. 2008. Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. Journal Agriculture Food Chemical 56:139–147. DOI: 10.1021/jf072304b
  23. Molyneux, P. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26:211–219. DOI: 10.1287/isre.6.2.144
  24. Pangesti, Y.D, Parnanto, N.H.R., Achmad, R.A. 2014. Kajian sifat fisikokimia tepung bengkuang (pachyrhizus erosus) dimodifikasi secara heat moisture treatment (HMT) dengan variasi suhu. Jurnal Teknosains Pangan 3(3):72–77
  25. Pellegrini, N., Miglio, C., Rio, D., Salvatore, S., Serafini, M., Brighenti, F. 2009. Effect of domestic cooking methods on the total antioxidant capacity of vegetables. International Journal of Food Sciences and Nutrition 60 Suppl 2:12-22. DOI: 10.1080/09637480802175212
  26. Roy, A., Shrivastava, S.L., Mandal, S.M. 2014. Functional properties of Okra Abelmoschus esculentus L. (Moench): traditional claims and scientific evidences. Plant Science Today 1:121–130. DOI: 10.14719/pst.2014.1.3.63
  27. Sa’eed, H.B., Neela, B. 2016. Nutrient profile, bioactive components, and functional properties of Okra (Abelmoschus esculentus (L.) Moench). Bioactive Foods in Health Promotion. Academic Press, US. Page 365–409. DOI: 10.1016/B978-0-12-802972-5.00018-4
  28. Sabitha V., Ramachandran S., Naveen K.R., Panneerselvam K. 2012. Investigation of in vivo antioxidant property of Abelmoschus esculentus (L) Moench fruit seed and peel powders in streptozotocin induced diabetic rats. Journal of Ayurveda & Integrative Medicine 3(4):188–193. DOI: 10.4103/0975-9476.104432
  29. Sacks, E.J., Francis, D.M. 2001. Genetic and Environmental Variation for Tomato Flesh Color in a Population of Modern Breeding Lines. Journal of the American Society for Horticultural Science 126(2):221–226. DOI: 10.21273/JASHS.126.2.221
  30. Sheu, S.C., Lai, M.H. 2012. Composition analysis and immuno-modulatory effect of Okra (Abelmoschus esculentus L.) extract. Food Chemical 134(4):1906–1911. DOI: 10.1016/j.foodchem.2012.03.110
  31. Suica-Bunghez, I.R., Teodorescu, S., Dulama, I.D., Voinea, O.C., Simionescu, S., Ion, R.M. 2016. Antioxidant activity and phytochemical compounds of snake fruit (Salacca zalacca). International Conference on Innovative Research 133:1–8. DOI: 10.1088/1757-899X/133/1/012051
  32. Turkmen, N., Sari, F., Velioglu, Y.S. 2005. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry 93: 713–718. DOI: 10.1016/j.foodchem.2004.12.038
  33. Ubwa, S.T., Tyohemba, R.L. Oshido, B.A., Amua, Q.M. 2014. Chemical analysis of some wild underutilized mucilaginous vegetables and a domesticated vegetable in benue state, Nigeria. British Journal of Applied Science & Technology 4(32):4566–4574. DOI: 10.9734/BJAST/2014/12241
  34. Zhang, Z.L. 2014. Antioxidative and glucose homeostatic effects of extracts and isolated components from Okra (Abelmoschus esculentus Fruits). Molecules 24(1)38–50. DOI: 10.1016/j.jnutbio.2014.02.010
  35. Zhu, Q.Y., Huang, Y., Chen, Z.Y. 2000. Interaction between flavonoids and α-tocopherol in human low density lipoprotein. Journal Nutrition Biochemical 11(1):14–21. DOI: 10.1016/S0955-2863(99)00065-0

Last update:

No citation recorded.

Last update:

No citation recorded.