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Optimasi Formula MP-ASI Bubuk Instan Sumber Protein dengan Subtitusi Hidrolisat Protein Ikan (HPI) dan Tepung Kacang Hijau Menggunakan Response Surface Methodology (RSM)

*Yosi Irene Putri  -  Program Studi Magister Ilmu gizi, Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
Syaiful Anwar  -  Program Studi Agroekoteknologi, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, Indonesia
Diana Nur Afifah  -  Departemen Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro, Semarang, Indonesia
Ekowati Chasanah  -  Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta, Indonesia
Yusro Nuri Fawzya  -  Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta, Indonesia
Pujoyuwono Martosuyono  -  Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta, Indonesia

Citation Format:
Abstract

Abstrak

Penelitian ini bertujuan untuk mengoptimalkan formulasi bahan baku sumber protein pada Makanan Pendamping ASI (MP-ASI) untuk mendapatkan kandungan protein tertinggi. Metode eksperimen yang menggunakan 3-faktor 1-respon dengan menggunakan Response Surface Methodology (RSM). Tepung susu skim, bubuk Hidrolisat Protein Ikan (HPI), dan tepung kacang hijau dengan berbagai konsentrasi digunakan sebagai sumber bahan baku dan kandungan protein digunakan sebagai respon. Hasil dari respon dianalisis dengan analisis varians (ANOVA) dan melihat nilai desirability (optimasi terbaik). Formula MP-ASI dari hasil optimasi protein terbaik kemudian dilanjutkan dengan analisis proksimat. Hasil penelitian menunjukkan bahwa jenis bahan baku memiliki pengaruh terhadap kandungan protein (p<0,05). Tiga formula MP-ASI bubuk terbaik dari hasil optimasi adalah memiliki nilai desirability sebesar 0,714–0,791, kadar protein sebesar 18,72–19,38 g, kadar air berkisar 7,14–7,34 g, kadar abu berkisar 2,95–3,10 g dan kadar lemak berkisar 5,44–5,82 g dalam 100 g bubuk MP-ASI. Kesimpulannya, formula MP-ASI dapat ditentukan berdasarkan kadar protein tertinggi dan rasio bahan baku mempengaruhi kandungan nutrisi pada produknya 

Optimization of Formula for High Protein–Complementary Food using Fish Protein Hydrolysate and Mung Bean Flour by Response Surface Methodology

Abstract

This research aims to optimize the formula of material for protein sources to get the optimum response (based on protein content). The method of 3-factor and 1-response was used using Response Surface Methodology (RSM) to analyze the materials of skim milk flour (g, X1), Fish Protein Hydrolysate (FPH) flour (g, X2), and mung bean flour (g, X3) as independent variable/factor, whereas protein (g, Y) was used as dependent variable /response. The results were analyzed with variant analysis (ANOVA) and observed its desirability value to obtain best optimum value. The best of formula was examined its nutrition value using proximate analysis. This research showed that independent variables (X) had significant effect to the dependent variable (Y) (p<0.05). The best three formulas of high–protein complementary food had desirability value starting from 0.714 to 0.791. The proximate test resulted protein content of 18.72–19.38 g, moisture content of 7.14–7.34 g, ash content of 2.95–3.10 g, and fat content of 5.44–5.82 g. In conclusion, the best formula could be determined successfully using combinations of protein sources.

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Keywords: protein optimization; complementary food of instant flour; fish protein hydrolizate (FPH); Response Surface Methodology (RSM)
Funding: Insentif Riset Sistem Inovasi Nasional (INSINAS) penelitian atas nama Dr. Ekowati Chasanah, Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jakarta

Article Metrics:

  1. Aboud, F.E., Shafique, S., Akhter, S. 2009. Responsive feeding intervention increases children’s self–feeding and maternal responsiveness but not weight gain. The Journal of Nutrition 139:1738-1743. DOI: 10.3945/jn.109.104885
  2. Adawyah, R. 2014. Pengeringan dan pengawetan ikan, Sinar Grafika Offset, Jakarta
  3. Adu, O.B., Ogundeko, T.O., Ogunrinola, O.O., Saibu, G.M., Elemo, B.O. 2015. The effect of thermal processing on protein quality and free amino acid profile of Terminalia catappa (Indian Almond) seed. Journal of Food Science and Technology 52(7):4637–41. DOI: 10.1007/s13197-014-1490-8
  4. Anwar, F., Latif, S., Przybylski, R., Sultana, B., Ashraf, M. 2007. Chemical composition and antioxidant activity of seeds of different cultivars of mung bean. Journal of Food Science 72(7):S503-510. DOI: 10.111/j.1750-3841.2007.00460.x
  5. Aprilia, V., Hati, F.S. 2016. Formulasi bubur bayi MP-ASI yang diperkaya hidrolisat protein ikan lele dumbo (Clarias gariepinus). Jurnal Gizi dan Dietetik Indonesia 4(2): 88-96. DOI : 10.21927/ijnd.2016.4(2).88-96
  6. Asriyana, Irawati, N. 2018. Makanan dan strategi pola makan ikan kuniran Upeneus sulphureus, Cuvier (1829) di perairan Teluk Kendari, Sulawesi Tenggara. Jurnal Iktiologi Indonesia 18(1): 23-39 DOI: https://doi.org/10.32491/jii.v18i1.372
  7. Berk, Z. 2009. Food process engineering and technology (Dehydration), Elsevier Academic Press, Israel
  8. Bhandari, B., Bansal, N., Zhang, M., Schuck, P. 2013. Handbook of Food Powders: Infant Formula Powders, Woodhead Publishing, USA
  9. Chalamaiah, M., Hemalatha, R., Jyothirmayi, T. 2012. Fish protein hydrolysates: proximate composition amino acid composition, antioxidant activities and applications: a review. Food Chemistry 135(4):3020–3038. DOI: 10.1016/j.foodchem.2012.06
  10. Fikiru, O., Bultosa, G., Forsido, S.F., Temesgen, M. 2017. Nutritional quality and sensory acceptability of complementary food blended from maize (Zea mays), roasted pea (Pisum sativum), and malted barley (Hordium vulgare). Food Science and Nutrition 5(2):173–181. DOI: 10.1002/fsn3.376
  11. Hasim, Arifin, Y.Y., Andrianto, A., Faridah, D.N. Ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi) sebagai antioksidan dan antiinflamasi. Jurnal Aplikasi Teknologi Pangan 8(3):86:93. DOI: 10.17728/jatp.4201
  12. Lonnie, M., Hooker, E., Brunstrom, J.M., Corfe, B.M., Green, M.A., Watson, A.W., Williams, E.A., Stevenson, E.J., Penson, S., Johnstone, A.M. 2018. Protein for Life: Review of optimal protein intake, sustainable dietary sources and the effect on appetite in ageing adults. Nutrients 10:360. DOI: 10.3390/nu10030360
  13. Liang, N., Chen, X-M., Kitts, D.D. 2018. Sugar loss attributed to non-enzymatic browning corresponds to reduce calories recovered in low-molecular-weight fraction. Journal of Nutrition and Food Sciences 8(2):1-6. DOI: 10.4172/2155-9600.1000674
  14. Luque, V., Closa-Monasterolo, R., Escribano, J., Ferré, N. 2016. Early programming by protein intake: the effect of protein on adiposity development and the growth and functionality of vital organs. Nutrition and Metabolic Insights 8(S1):49-56. DOI: 10.4137/NMI.S29525
  15. Mourabet, M., Rhilassi, A.E., Boujaady, H.E., Bennani-Ziatni, M., Taitai, A. 2017. Use of response surface methodology for optimization of fluoride adsorption in an aqueous solution by Brushite. Arabian Journal of Chemistry 10:S3292–S302. DOI: 10.1016/j.arabjc.2013.12.028
  16. Naozuka, J., Oliveira, P.V. 2012. Cooking effects on iron and proteins content of beans (Phaseolus Vulgaris L.) by GF AAS and MALDI-TOF MS. Journal of the Brazilian Chemical Society 23(1):156-62. DOI: 10.1590/S0103-50532012000100022
  17. Nuriana, A., Aini, N., Karseno. 2019. Formulasi Breakfast Meal Flakes dari Tepung Suweg dan Stabilized Rice Bran Menggunakan Metode Respon Permukaan. Jurnal Aplikasi Teknologi Pangan 8(2):52–59. DOI: 10.17728/jatp.3952
  18. Pengkumsri, N., Chaiyasut, C., Saenjum, C., Sirilun, S., Peerajan, S., Suwannalert, P., Sirisattha, S., Sivamaruthi, B.S. 2015. Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand. Journal of Food Science 35(2):331- 338. DOI: 10.1590/1678-457X.6573
  19. Setyaningsih, D, Apriyantono, A., Puspitasari, M. 2010. Analisis sensori untuk industri pangan dan agro. IPB Press, Bogor
  20. Shevkani, K., Singh, N., Kaur, A., Rana, J.C. 2015. Structural and functional characterization of kidney bean and field pea protein isolates: a comparative study. Food Hydrocolloids 43:679–689. DOI: 10.1016/j.foodhyd.2014.07.024
  21. Standar Nasional Indonesia (SNI) 01-07111.1. 2005. Makanan Pendamping Air Susu Ibu (MP-ASI). 2005. Badan Standarisasi Nasional. Jakarta
  22. Standar Nasional Indonesia (SNI) 2354.4. 2006. Penentuan kadar protein dengan metode total nitrogen pada produk perikanan. Badan Standarisasi Nasional. Jakarta
  23. Standar Nasional Indonesia (SNI) 2354.1. 2010. Pengujian kadar abu pada produk perikanan. Badan Standarisasi Nasional. Jakarta
  24. Standar Nasional Indonesia (SNI) 2354.2. 2015 Pengujian kadar air pada produk perikanan. Badan Standarisasi Nasional. Jakarta
  25. Standar Nasional Indonesia (SNI) 2354.3. 2017 Penentuan kadar lemak pada produk perikanan. Badan Standarisasi Nasional. Jakarta
  26. Sudarmadji S, Haryono B, Suhardi.1997. Prosedur Analisis Untuk Bahan Makanan dan Pertanian, Liberty, Yogyakarta
  27. Syamaladevi, R.M., Tang, J., Villa-Rojas, R., Sablani, H, Carter, B., Campbell, G. 2016. Influence of water activity on thermalresistance of microorganisms in low-moisturefoods: a review. Comprehensive Reviews in Food Science and Food Safety 15:353-370. DOI: 10.1111/1541-4337.12190
  28. Tessema, M., Gunaratna, N.S., Brouwer, I.D., Donato, K., Cohen, J.L., McConnell, M., Belachew, T., Belayneh, D., Groote, H.D. 2018. Associations among high-quality protein and energy intake, serum transthyretin, serum amino acids and linear growth of children in ethiopia. Nutrients 10(1776): 4-17. DOI: 10.3390/nu10111776
  29. Uauy, R., Suri, D.J., Ghosh, S., Kurpad, A., Rosenbergd, I.H. 2016. Low circulating amino acids and protein quality: an interesting piece in the puzzle of early childhood stunting. EbioMedicine 8:28-29. DOI: 10.1016/j.ebiom.2016.05.026
  30. Usydus, Z., Szlinder-Richert, J., Adamczyk, M. 2009. Protein quality and amino acid profiles of fish products available in Poland. Food Chemistry 112:139–45. DOI: 10.1016/j.foodchem.2008.05.050
  31. Wen-qian, J., Fu-chang, L. 2010. Effects of dietary lysine on growth performance, serum concentrations of Insulin-Like Growth Factor-I (IGF-I) and IGF-I mRNA expression in growing rabbits. Agricultural Sciences 9(6):887-95. DOI: 10.1016/S1671-2927(09)60168-1
  32. WHO. 2001. Complementary feeding: report of the global consultation, and summary of guiding principles for complementary feeding of the breastfed child. Convened jointly by the Department of Child and Adolescent Health and Development and the Department of Nutrition for Health and Development, WHO Library Cataloguing-in-Publication Data, Geneva
  33. WHO. 2009. Infant and young child feeding: model chapter for textbooks for medical student and allied health professionals, WHO Press, World Health Organization
  34. Xu, X.P., Liu, H., Tian, L., Dong, X.B., Shen, S.H., Qu le, Q. 2015. Integrated and comparative proteomics of high-oil and high-protein soybean seeds. Food Chemistry 172:105–116 DOI: 10.1016/j.foodchem.2014.09.035

Last update: 2021-07-27 06:56:33

No citation recorded.

Last update: 2021-07-27 06:56:33

No citation recorded.