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Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi

*Cahya Setya Utama  -  Departemen Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, Indonesia
Zuprizal Zuprizal  -  Departemen Nutrisi dan Makanan Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia
Chusnul Hanim  -  Departemen Nutrisi dan Makanan Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia
Wihandoyo Wihandoyo  -  Departemen Produksi Ternak Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia

Citation Format:
Abstract

Penelitian bertujuan mengisolasi dan identifikasi jenis bakteri asam laktat (BAL) selulolitik yang berasal dari jus kubis terfermentasi. Tahapan penelitian meliputi isolasi mikrobia dari jus kubis terfermentasi, karakteristik morfologi sel, karakteristik biokimiawi, identifikasi dengan kit api 50 CHL dan pengujian kemampuan mendegradasi selulosa. Penelitian diawali dengan memfermentasi jus kubis selama 6 hari pada kondisi anaerobic fakultatif. Hasil fermentasi diisolasi dengan media de man rogosa and sharpe (MRS) yang ditambahkan CaCO3 1% dan diinkubasi selama 24 jam. Reinokulasi dilakukan sebanyak 5 kali sampai ditemukan kultur murni BAL, kemudian diidentifikasi dengan menggunakan kit analytical profile index (API) 50 CHL. Hasil identifikasi kemudian diuji kemampuannya untuk mendegradasi selulosa pada media MRS yang ditambahkan 1% carboxymethyl cellulosa (CMC) dan 0,1% congored sebagai indikator dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan bahwa strain bakteri yang teridentifikasi adalah Lactobacillus plantarum dan Lactobacillus brevis yang dapat mendegradasi selulosa. Kesimpulan dari penelitian ini adalah ditemukannya 2 jenis bakteri asam laktat dari jus kubis terfermentasi yaitu Lactobacillus plantarum dan Lactobacillus brevis yang mempunyai sifat selulolitik.

Isolation and Identification of Lactic Acid Bacteria Cellulolitik Originated from Fermented Cabbage Juice

Abstract

The objective of this study was to isolate and identify the type of lactic acid bacteria (BAL) of cellulolytic originating from fermented cabbage juice. Research was conducted with the following steps: microbial isolation from fermented cabbage juice, cell morphology characteristics, biochemical characteristics, identification with an analytical profile index (API) 50 CHL kit, and cellulose degradability testing. The research was begun by fermenting cabbage juice for 6 days under facultative aerobic condition. The fermentation product was then isolated with de man rogosa and sharpe (MRS) medium which added CaCO3 1% and incubated for 24 hours. Reinoculation was performed 5 times until BAL was found. BAL pure cultures were used to identify lactic acid bacteria strains using an analytical profile index (API) 50 CHL kit. After pure culture was found, it was then tested for the ability to degrade cellulose on MRS media with 1% carboxymethyl cellulose (CMC) and 0.1% congored as indicator and incubated for 24 hours. The results showed that the identified bacterial strains were Lactobacillus plantarum and Lactobacillus brevis which could degrade cellulose. The conclusion of this research was the discovery of two types of lactic acid bacteria from fermented cabbage juice namely Lactobacillus plantarum and Lactobacillus brevis which had cellulolytic properties.

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Keywords: kubis; fermentasi; isolasi; identifikasi; bakteri asam laktat; selulosa

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  1. Alam, M. Z., Manchur, M. A., Anwar, M. N. 2004. Isolation, purification, characterization of cellulolytic enzymes produced by Streptomycesomiyaensin. Journal of Biologi Science 10:1647- 1653
  2. Ammor. M. S., Mayo, B. 2007. Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production. Meat. Sci. 76(1): 138–146. DOI: 10.1111/j.1574-6976.1993.tb00022.x
  3. Axelsson, L. 1998. Lactid acid bacteria: clasification and physiology. In: Salminen S,Wright AV (eds). Lactid acid bacteria: microbiology and functional aspects. Marcel Dekker, New York
  4. Bacus, J. 1984. Utilization of Microorganism in Meat Processing. Research Studies Press. Letchworth, Herts, England
  5. Biomerieux. 2009. API 50CHL medium for invitro diagnostic use. http://www.biomerieux.com
  6. Boekhorst, J., Wels, M., Kleerebezem, M., Siezen, R. J. 2006. The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment. Microbiology. 152:3175-3183. DOI: 10.1099/mic.0.29217-0
  7. Carr, F., Chill, J. D., Maida, N. 2002. The Lactic Acid Bacteria: A Literature Survey. Critical Reviews Microbiology 28(4):281–370. DOI: 10.1080/1040-840291046759
  8. Chu, Y. F., Sun, J., Wu, X., Liu, R. H. 2002. Antioxidants and antiproliferative activities of vegetables. J. Agri. Food. Chem. 50:6910-6916. DOI: 10.1021/jf020665f
  9. Harrigan, W. F. 1998. Laboratory Methods in Food Microbiology. Academic Press, Inc, New York
  10. Hartadi, H., Reksohadiprodjo, S., Tillman. A.D. 1997. Tabel Komposisi Pakan Ternak untuk Indonesia. Cetakan keempat. Gadjah Mada University Press
  11. Holt, J.G., Krieg, N.R., Peter, H.A., Staley, J.T., Williams, S.T. 1994. Bergey’s Manualof Determinative Bacteriology Ninth edition. Williams & Wilkins. Baltimore, Maryland. USA
  12. Krabi, R. E., Assamoi, A. A., Ehon, F. A., Niamke. 2015. Screening of lactic acid bacteria as potential starter for the production of attieke, a fermented cassava food. Journal of Faculty of Food Engineering. 14(1):21-29
  13. Meryandini, A., Widosari, W., Maranatha, B., Sunarti, T. C., Rachmania, N., Satria, H. 2009. Isolasi bakteri selulolitik dan karakterisasi enzimnya. Makara sains. 13(1):33-38. DOI: 10.7454/mss.v13i1.369
  14. Molenaar, D., Bringel, F., Schuren, F. H., de Vos, W. M., Siezen, R. J., Kleerebezem, M. 2005. Exploring Lactobacillus plantarum genome diversity by using microarrays. J. Bacteriol. 187: 6119-6127. DOI: 10.7454/mss.v13i1.369
  15. Murtidjo, B. A. 1993. Pedoman Meramu Pakan Unggas. Cetakan Kelima. Kanisius, Yogyakarta
  16. Narasimha, G. 2005. Nutrient effects on productions of cellulolytic enzymes by Aspergillus niger. Journal Biotechnol 5: 472-476. DOI: 10.1128/JB.187.17.6119-6127.2005
  17. Pelczar, M. J., Chan, E. C. S. 1988. Dasar-Dasar Mikrobiologi 2. Universitas Indonesia Press, Jakarta (Diterjemahkan oleh Hadiutomo, Imas, Tjitrosomo dan Angka)
  18. Plengvidhya, V.F., Breidt, F., Lu, Z., Fleming, H. P. 2007. DNA fingerprinting of lactic acid bacteria in sauerkraut fermentations. Appl. Environ. Microbiol. 73:7697–7702. DOI: 10.1128/AEM.01342-07.PMID17921264
  19. Schlegel, H. G. 1995. Mikrobiologi Umum. Edisi keenam. Diterjemahkan oleh Baskoro. Gadjah Mada University Press. Yogyakarta
  20. Suardana, I. W., Suarsana, I. N., Sujaya, I. N., Wiryawan, K.G. 2007. Isolasi dan Identifikasi bakteri asam laktat dari cairan rumen sapi bali sebagai kandidat biopreservatif. Jurnal Veteriner. 8(4): 155–159. https://ojs.unud.ac.id/index.php/jvet/article/view/3217
  21. Sujaya N., Ramona, Y., Widarini, N.P., Suariani., N. P., Dwipayanti, N. M. U, Nocianitri, K. A., Nursini, N. W. 2008. Isolasi dan karakterisasi bakteri asam laktat dari susu kuda Sumbawa. Jurnal Veteriner. 9(2):52-59. https://ojs.unud.ac.id/index.php/jvet/article/view/3314/2360
  22. Utama, C. S., Mulyanto, A. 2009. Potensi limbah pasar sayur menjadi supplemen fermentasi. Jurnal Kesehatan Unimus. 2(1):6-13
  23. Volk, W. A.,Wheeler. 1992. Mikrobiologi Dasar. Jilid 2. Edisi Kelima. Erlangga, Jakarta
  24. Walter, H. G., Kohler, G.O. 1978. Treated and untreated cellulotic wastes and animal feeds. Recents Work Interaksi The United States of America, USA
  25. Zubaidah, E., Saparianti, E., Hindrawan, J. 2012. Studi aktivitas antioksidan pada bekatul dan susu skim terfermentasi probiotik Lactobacillus plantarum B2 dan Lactobacillus acidophilus. Jurnal Teknologi Pertanian 3(2):111-118

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