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Characterization of Phospholipids From Sesame seeds

Aniatun Linafi'ah  -  Chemistry Postgraduate Program, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
*Dwi Hudiyanti scopus  -  Department of Chemistry, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
Parsaoraan Siahaan scopus  -  Department of Chemistry, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

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Abstract
The aim of the study was to determine the characteristic of sesame phospholipids and to determine the quality of sesame phospholipids based on the value of peroxide number and antioxidant activity. Phospholipids can be used as active encapsulating agents such as beta-carotene and vitamin C. Phospholipids can be used as a method of liquid extraction and solvent evaporation. The yield of phospholipids obtained was 0,475%. The color result was brownish yellow. The test characteristic consisted of a proximate analysis between sesame seeds and phospholipids. Sesame seeds are dominated by the content of crude fiber, which is 73.152%, while in phospholipids, crude fat content is dominated by 96%. The testing of antioxidants and peroxide values with samples of sesame, the extract of total lipid, and phospholipids on the seventh and fourteenth days of storage. This test is used to determine the quality of the basic ingredients used for phospholipid isolation and to determine the best quality of phospholipids based on the day of storage. The research showed that the antioxidant activity testing for sesame samples and total sesame extract on the fourteenth day were 25,699  % and 28,562%. The highest antioxidant phospholipid activity on the seventh day was 34,160 %, while on the fourteenth day the antioxidant activity was 30,979 %. In the test, the peroxide number showed the highest value on the fourteenth day in the third sample, namely the sesame seed sample, total lipid extract, and phospholipids, respectively, 0.1784  mgO2/g, 0.1675 mgO2/g, and 0.1675 mgO2/g. 3, 1988  mgO2/g. When compared with soy lecithin based on SNI, the peroxide value is 5, so on the fourteenth day of storage, sesame phospholipids are in good quality.
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Keywords: sesame, phospholipids, peroxide value, antioxidant activity

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