The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models

*Puspita Kusuma Dewi orcid  -  Faculty of Medicine, Diponegoro University, Indonesia
Maharani Cahyono  -  Faculty of Medicine, Diponegoro University, Indonesia
Riski Prihatningtias  -  Faculty of Medicine, Diponegoro University, Indonesia
Liana Ekowati  -  Faculty of Medicine, Diponegoro University, Indonesia
Arief Wildan  -  Faculty of Medicine, Diponegoro University, Indonesia
Received: 19 Nov 2020; Revised: 17 Dec 2020; Accepted: 26 Dec 2020; Published: 31 Dec 2020; Available online: 31 Dec 2020.
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Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models.

Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit.

Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001).

Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.

Note: This article has supplementary file(s).

Keywords: Retinopathy of prematurity; glutathione; malondialdehyde

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  1. .Lueder G AS, Hered R, Karr D, Kodsi S. Pediatric ophthalmology and strabismus, Basic and Clinical Science Course. San Fransisco: The American Academy of Ophthalmology; 2017. p. 321
  2. Couroucli XI. Oxidative stress in the retina: implications for Retinopathy of Prematurity. Current Opinion in Toxicology. 2017
  3. Keles S, Caner I, Ates O, Cakici O, Saruhan F, Mumcu UY, et al. Protective effect of L-carnitine in a rat model of retinopathy of prematurity. Turkish Journal of medical sciences. 2014;44(3):471-5
  4. Edy Siswanto J, Sauer PJ. Retinopathy of prematurity in Indonesia: incidence and risk factors. Journal of neonatal-perinatal medicine. 2017;10(1):85-90
  5. Wang H. Anti-VEGF therapy in the management of retinopathy of prematurity: what we learn from representative animal models of oxygen-induced retinopathy. Eye and brain. 2016;8:81
  6. Giblin FJ. Glutathione: a vital lens antioxidant. Journal of Ocular Pharmacology and Therapeutics. 2000;16(2):121-35
  7. Sankar MJ SJ. Vitamin E supplementation for prevention of morbidity and mortality in preterm infants [internet]: WHO Reproductive Health Library; 2011 [updated August 2011; cited 2018 November 15]. Available from:
  8. Beharry KD, Valencia GB, Lazzaro DR, Aranda JV, editors. Pharmacologic interventions for the prevention and treatment of retinopathy of prematurity. Seminars in perinatology; 2016: Elsevier
  9. Dorfman AL. Oxygen Induced Retinopathy in the Neonatal Rat: The Effects of Age, Strain and Therapeutic Intervention on Retinal Structure and Function [dissertation]. Montreal: McGill University; 2010
  10. Ahmadvand H, Babaeenezhad E, Nasri M, Jafaripour L, Khorramabadi RM. Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury. Journal of Renal Injury Prevention. 2019;8(2):91-7
  11. Weschawalit S, Thongthip S, Phutrakool P, Asawanonda P. Glutathione and its antiaging and antimelanogenic effects. Clinical, cosmetic and investigational dermatology. 2017;10:147
  12. Nagar A. Protective effect of glutathione against isoproterenol induced myocardial injury in rats. Indian J Physiol Pharmacol. 2013;57(2):132-7
  13. Wang J, Chen Y, Gao N, Wang Y, Tian Y, Wu J, et al. Inhibitory effect of glutathione on oxidative liver injury induced by dengue virus serotype 2 infections in mice. PloS one. 2013;8(1):e55407
  14. Sutaryono S, Andasari SD, Hidayati N. Pengaruh Pemberian Campuran Bee Pollen, Rimpang Kencur, Kunyit dan Biji Pinang Terhadap Penurunan Kadar Malondialdehida (MDA) pada Tikus Wistar Pasca Paparan Streptozotocin. 2016
  15. Boyd K. What is Retinopathy of Prematurity (ROP)? [internet].2018 [updated April 28, 2018; cited 2018 November 13]. Available from:
  16. Retinopathy of Prematurity - Asia Pacific [internet].2013 [updated November 2013; cited 2018 november 13]. Available from:
  17. Stuart A. Current ROP Therapies: How Laser and Anti-VEGF Compare 2014
  18. Garg U, Jain A, Singla P, Beri S, Garg R, Saili A. Free radical status in retinopathy of prematurity. Indian Journal of Clinical Biochemistry. 2012;27(2):196-9
  19. Lee JW, Davis JM. Future applications of antioxidants in premature infants. Current opinion in pediatrics. 2011;23(2):161
  20. Kim Y-W, Byzova TV. Oxidative stress in angiogenesis and vascular disease. Blood. 2014;123(5):625-31

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