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The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 8 Mar 2022; Revised: 25 Apr 2022; Accepted: 14 Jun 2022; Available online: 31 Aug 2022; Published: 31 Aug 2022.
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Background: The extracellular matrix (ECM) in the trabecular meshwork (TM) may play a role in the steroid-induced aqueous humor outflow resistance pathophysiology. Topical steroids such as fluorometholone can cause elevated intraocular pressure. Increased synthesis and accumulation of TM ECM proteins due to steroids result in TM dysfunction and elevated intraocular pressure.

Purpose: This study aims to analyze the effect of duration of administration and discontinuation of fluorometholone eye drops on the thickness of the TM ECM of Wistar rats.

Methods: This study was an experimental study with a posttest-only control group design. The total sample of 25 rats was divided into 5 groups: Treatment 1 was given topical fluorometholone for 4 weeks and stopped for 4 weeks. Treatment 2 was given fluorometholone for 6 weeks and stopped for 4 weeks. Control 1 was given topical fluorometholone for 4 weeks, Control 2 was given topical fluorometholone for 6 weeks, and the negative control was used as a baseline. TM ECM examination was assessed using histopathological score grading. This study used the Mann-Whitney test and the Kruskal-Wallis comparative hypothesis test.

Results: The difference in ECM thickness was not statistically significant either between treatment group 1 and treatment group 2 (p=1.000) or control group 1 (p=0,1000). There was a significant change in the thickness of the TM ECM in treatment group 1, treatment 2, control 1, control 2 compared to the negative control (p=0.003, p=0.003, p=0.003, p=0.004).

Conclusion: TM ECM thickness did not return to normal thickness after administration of fluorometholone eye drops one drop 4 times a day for 4 weeks and 6 weeks followed by discontinuation of the drug for 4 weeks. The thickness of the TM ECM was thicker in experimental rats with a longer duration of administration of fluorometholone eye drops.

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Keywords: Extracellular matrix; Fluorometholone; Trabecular meshwork
Funding: Faculty of Medicine Diponegoro University Semarang

Article Metrics:

  1. Phulke S, Kaushik S, Kaur S, Pandav SS. Steroid-induced Glaucoma: An Avoidable Irreversible Blindness. Journal of current glaucoma practice. 2017;11(2):67–72.
  2. Mandelkom R. Drug induced Glaucoma, clinical pathway in glaucoma, in : Zimmerman and Kooner. New york : Thieme Medical Publishers inc. 2001;350–333
  3. Wordinger RJ, Clark AF. Effects of glucocorticoids on the trabecular meshwork: towards a better understanding of glaucoma. Progress in Retinal and Eye Research. 1999 Apr;18(5):629–67.
  4. Qin Y, Lam S, Yam GHF, Choy KW, Liu DTL, Chiu TYH, et al. A rabbit model of age-dependant ocular hypertensive response to topical corticosteroids. Acta Ophthalmologica. 2012 Sep;90(6):559–63.
  5. Overby DR, Clark AF. Animal models of glucocorticoid-induced glaucoma. Experimental Eye Research. 2015 Dec;141:15–22.
  6. Spiers F. Topical steroids and intraocular pressure. I. Clinical investigation on the reactions of 93 outpatients to monocular steroid provocation and to subsequent water-drinking test. Acta ophthalmologica. 1965;43(5):735–45
  7. Raghunathan VK, Benoit J, Kasetti R, Zode G, Salemi M, Phinney BS, et al. Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior. Acta Biomaterialia. 2018 Apr;71:444–59.
  8. Li B, Erica P, David C. Prednisolone Induces Time- And Dose-dependent Ocular Hypertension In Rats. Invest Ophthalmol Vis Sci. 2012;53(14)
  9. Sekar Kumalasari, Fifin LR, Arnila NS.(2021). Pengaruh Durasi Penghentian Prednisolon Topikal Terhadap Ketebalan Matrik Ekstra Selular Trabecular Meshwork. Thesis, Faculty of Medicine, Diponegoro University

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