The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork

Sandi Muslim; Arnila Novitasari Saubig; Riski Prihatningtias; A. Kentar Arimadyo Sulakso; Fifin Luthfia Rahmi

doi:10.14710/jbtr.v8i2.13817

DOI: https://doi.org/10.14710/jbtr.v8i2.13817

The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork

Sandi Muslim , Arnila Novitasari Saubig, Riski Prihatningtias, A. Kentar Arimadyo Sulakso, Fifin Luthfia Rahmi

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 8 Mar 2022; Revised: 25 Apr 2022; Accepted: 14 Jun 2022; Available online: 31 Aug 2022; Published: 31 Aug 2022.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

BibTex Citation Data :

@article{JBTR13817,
    author = {Sandi Muslim and Arnila Saubig and Riski Prihatningtias and A. Kentar Sulakso and Fifin Rahmi},
    title = {The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork},
    journal = {Journal of Biomedicine and Translational Research},
  volume = {8},
    number = {2},
    year = {2022},
    keywords = {Extracellular matrix; Fluorometholone; Trabecular meshwork},
    abstract = {  Background  :  The extracellular matrix (ECM) in the trabecular meshwork (TM) may play a role in the steroid-induced aqueous humor outflow resistance pathophysiology. Topical steroids such as fluorometholone can cause elevated intraocular pressure. Increased synthesis and accumulation of TM ECM proteins due to steroids result in TM dysfunction and elevated intraocular pressure.   Purpose  :    This study aims to analyze the effect of duration of administration and discontinuation of fluorometholone eye drops on the thickness of the TM ECM of Wistar rats .    Methods  :  This study was an experimental study with a posttest-only control group design. The total sample of 25 rats was divided into 5 groups: Treatment 1 was given topical fluorometholone for 4 weeks and stopped for 4 weeks. Treatment 2 was given fluorometholone for 6 weeks and stopped for 4 weeks. Control 1 was given topical fluorometholone for 4 weeks, Control 2 was given topical fluorometholone for 6 weeks, and the negative control was used as a baseline. TM ECM examination was assessed using histopathological score grading. This study used the Mann-Whitney test and the Kruskal-Wallis comparative hypothesis test.   Results:  The difference in ECM thickness was not statistically significant either between treatment group 1 and treatment group 2 (p=1.000) or control group 1 (p=0,1000). There was a significant change in the thickness of the TM ECM in treatment group 1, treatment 2, control 1, control 2 compared to the negative control (p=0.003, p=0.003, p=0.003, p=0.004).   Conclusion  :  TM ECM thickness did not return to normal thickness after administration of fluorometholone eye drops one drop 4 times a day for 4 weeks and 6 weeks followed by discontinuation of the drug for 4 weeks. The thickness of the TM ECM was thicker in experimental rats with a longer duration of administration of fluorometholone eye drops. },
   issn = {2503-2178},   pages = {59--62}  doi = {10.14710/jbtr.v8i2.13817},
    url = {https://ejournal2.undip.ac.id/index.php/jbtr/article/view/13817}
}

Citation Format:

Abstract

Background: The extracellular matrix (ECM) in the trabecular meshwork (TM) may play a role in the steroid-induced aqueous humor outflow resistance pathophysiology. Topical steroids such as fluorometholone can cause elevated intraocular pressure. Increased synthesis and accumulation of TM ECM proteins due to steroids result in TM dysfunction and elevated intraocular pressure.

Purpose: This study aims to analyze the effect of duration of administration and discontinuation of fluorometholone eye drops on the thickness of the TM ECM of Wistar rats.

Methods: This study was an experimental study with a posttest-only control group design. The total sample of 25 rats was divided into 5 groups: Treatment 1 was given topical fluorometholone for 4 weeks and stopped for 4 weeks. Treatment 2 was given fluorometholone for 6 weeks and stopped for 4 weeks. Control 1 was given topical fluorometholone for 4 weeks, Control 2 was given topical fluorometholone for 6 weeks, and the negative control was used as a baseline. TM ECM examination was assessed using histopathological score grading. This study used the Mann-Whitney test and the Kruskal-Wallis comparative hypothesis test.

Results: The difference in ECM thickness was not statistically significant either between treatment group 1 and treatment group 2 (p=1.000) or control group 1 (p=0,1000). There was a significant change in the thickness of the TM ECM in treatment group 1, treatment 2, control 1, control 2 compared to the negative control (p=0.003, p=0.003, p=0.003, p=0.004).

Conclusion: TM ECM thickness did not return to normal thickness after administration of fluorometholone eye drops one drop 4 times a day for 4 weeks and 6 weeks followed by discontinuation of the drug for 4 weeks. The thickness of the TM ECM was thicker in experimental rats with a longer duration of administration of fluorometholone eye drops.

Note: This article has supplementary file(s).

Fulltext View|Download | Cover Letter

Cover Letter

Subject
Type	Cover Letter
	Download (15KB) Indexing metadata

CONFLICT OF INTEREST DECLARATION AND COPYRIGHT TRANSFER AGREEMENT FORM

Subject
Type	Copyright Transfer Agreement
	Download (259KB) Indexing metadata

Keywords: Extracellular matrix; Fluorometholone; Trabecular meshwork

Funding: Faculty of Medicine Diponegoro University Semarang

Article Metrics:

Article Info

Section: Original Research Articles

Language : EN

In Vol 8, No 2 (2022): August 2022

Recent articles

Negative correlation of Modified Sperm Stress Test with Leukocyte in Sperm but not with the sperm concentration and motility Correlation between Interleukin-17 Levels with C-Reactive Protein and Neutrophil Lymphocyte Ratio in Sepsis The Effect of Duration of Administration and Discontinuation of Fluorometholone Eye Drops on The Trabecular Meshwork Comparison of Testosterone Undecanoate (TU) and Testosterone Enanthate (TE) with Combination of Depot Medroxyprogesterone Acetate (DMPA) on Spermatogenic Cells in Men More recent articles

Phulke S, Kaushik S, Kaur S, Pandav SS. Steroid-induced Glaucoma: An Avoidable Irreversible Blindness. Journal of current glaucoma practice. 2017;11(2):67–72. https://dx.doi.org/10.5005%2Fjp-journals-l0028-1226
Mandelkom R. Drug induced Glaucoma, clinical pathway in glaucoma, in : Zimmerman and Kooner. New york : Thieme Medical Publishers inc. 2001;350–333
Wordinger RJ, Clark AF. Effects of glucocorticoids on the trabecular meshwork: towards a better understanding of glaucoma. Progress in Retinal and Eye Research. 1999 Apr;18(5):629–67. https://doi.org/10.1016/s1350-9462(98)00035-4
Qin Y, Lam S, Yam GHF, Choy KW, Liu DTL, Chiu TYH, et al. A rabbit model of age-dependant ocular hypertensive response to topical corticosteroids. Acta Ophthalmologica. 2012 Sep;90(6):559–63. https://doi.org/10.1111/j.1755-3768.2010.02016.x
Overby DR, Clark AF. Animal models of glucocorticoid-induced glaucoma. Experimental Eye Research. 2015 Dec;141:15–22. https://dx.doi.org/10.1016%2Fj.exer.2015.06.002
Spiers F. Topical steroids and intraocular pressure. I. Clinical investigation on the reactions of 93 outpatients to monocular steroid provocation and to subsequent water-drinking test. Acta ophthalmologica. 1965;43(5):735–45
Raghunathan VK, Benoit J, Kasetti R, Zode G, Salemi M, Phinney BS, et al. Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior. Acta Biomaterialia. 2018 Apr;71:444–59. https://dx.doi.org/10.1016%2Fj.actbio.2018.02.037
Li B, Erica P, David C. Prednisolone Induces Time- And Dose-dependent Ocular Hypertension In Rats. Invest Ophthalmol Vis Sci. 2012;53(14)
Sekar Kumalasari, Fifin LR, Arnila NS.(2021). Pengaruh Durasi Penghentian Prednisolon Topikal Terhadap Ketebalan Matrik Ekstra Selular Trabecular Meshwork. Thesis, Faculty of Medicine, Diponegoro University

Last update:

No citation recorded.

Last update:

No citation recorded.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Journal of Biomedicine and Translational Research Diponegoro University as publisher of the journal.

Copyright encompasses non-exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.

Journal of Biomedicine and Translational Research Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Journal of Biomedicine and Translational Research Diponegoro University (JBTR) are sole and exclusive responsibility of their respective authors and advertisers.

The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form JBTR]

The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax :
Journal of Biomedicine and Translational Research Faculty of Medicine, Diponegoro University
Jl. Prof. Soedarto, Kampus UNDIP Tembalang, Semarang, Central Java, Indonesia 50275, Telp.: +62-24-8454714, Fax.: +62-24-8454714
Email: jbtr@fk.undip.ac.id