Evaluasi Penetapan Kadar Kalsium pada Minuman Yogurt secara Titrasi Kelatometri

*Moh Taufik -  Program Studi Ilmu dan Teknologi Pangan, Fakultas Bioindustri, Universitas Trilogi, Jakarta, Indonesia
Seveline Seveline -  Program Studi Ilmu dan Teknologi Pangan, Fakultas Bioindustri, Universitas Trilogi, Jakarta, Indonesia
Maya Adriyanti -  Program Studi Ilmu dan Teknologi Pangan, Fakultas Bioindustri, Universitas Trilogi, Jakarta, Indonesia
Received: 4 Jan 2018; Published: 28 Feb 2018.
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Abstract

Titrasi kelatometri merupakan salah satu metode analisis kuantitatif yang dapat digunakan untuk menganalisa kadar kalsium. Sampai saat ini, belum ada data validasi analisis kadar kalsium secara titrasi kelatometri pada sampel minuman yogurt. Tujuan dari penelitian ini yaitu mengevaluasi metode penetapan kadar kalsium secara titrasi kelatometri pada sampel minuman yogurt. Hasil uji recovery menunjukkan bahwa metode titrasi kelatometri mempunyai nilai akurasi yang baik, yaitu 100,94% pada konsentrasi spiking larutan standar kalsium sebesar 60 mg/100 ml sampel minuman yogurt. Ripitabilitas dan intra-lab reprodusibilitas menunjukkan hasil yang dapat diterima dengan nilai relative standard deviation (RSD) berturut-turut sebesar 1,61% dan 1,74%. Hasil uji ketangguhan metode menunjukkan bahwa penggunaan sampel awal 5,0-10,0 ml menghasilkan kadar kalsium yang sama. Berdasarkan hasil ini, metode analisis kadar kalsium secara titrasi kelatometri dapat diaplikasikan pada sampel minuman yogurt.

Chelatometric titration is one of the quantitative analysis methods that can be used to analyze calcium content. This research aims to evaluate the analytical performance of calcium content analysis in the yogurt drink using chelatometric titration. The results showed that this chelatometric titration had a good accuracy, i.e. 100,94% at spiking concentration of 60 mg /100 ml sample. Repeatability and intra-lab reproducibility indicated acceptable results with relative standard deviation (RSD) values of 1,61% and 1,74%, respectively. The ruggedness test also indicated that chelatometric titration was tough to the volume of initial sample used, i.e. 5,0 ml to 10,0 ml. Based on these results, calcium content analysis using chelatometric titration can be applied in yogurt drink sample.

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Keywords
Evaluasi metode; kadar kalsium; minuman yogurt; titrasi kelatometri

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