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@article{JITPI8089, author = {Frisilia Rimadhini and Sumardianto Sumardianto and Romadhon Romadhon}, title = {AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ASAM LAKTAT DARI RUSIP IKAN TERI (Stolephorus sp.) DENGAN KONSENTRASI GULA AREN CAIR YANG BERBEDA}, journal = {Jurnal Ilmu dan Teknologi Perikanan}, volume = {2}, number = {1}, year = {2020}, keywords = {ikan teri, rusip, gula aren cair, bakteri asam laktat, antibakteri}, abstract = {Rusip adalah produk fermentasi ikan teri dengan gula aren dan garam yang menghasilkan. Gula aren pada rusip digunakan sebagai sumber energi untuk pertumbuhan. BAL berpotensi menghambat bakteri patogen produk perikanan. Penelitian ini bertujuan untuk mengisolasi BAL dengan perbedaan konsentrasi gula aren cair dan aktivitas antibakteri terhadap S. aureus dan E. coli. Metode penelitian yang digunakan adalah experimental laboratories dengan Rancangan Acak Lengkap (RAL). Perlakuan yang diterapkan adalah penambahan konsentrasi gula aren cair 5%; 7,5%; 10%; 12,5% dan 15% dengan tiga kali pengulangan. Data dianalisis menggunakan uji sidik ragam (ANOVA) yang dilanjut dengan uji Beda Nyata Jujur. Pengujian yang dilakukan antara lain pH, aw, TPC BAL, uji morfologi, pewarnaan gram dan katalase serta antibakteri. Hasil penelitian menunjukkan bahwa penambahan konsentrasi gula aren cair terbaik adalah 10% dengan pH 6,25, nilai aw 0,77 dan TPC BAL 2,4x105 cfu/g. Hasil uji morfologi, pewarnaan gram dan uji katalase dari 20 isolat BAL adalah 15 isolat berbentuk bulat, berwarna ungu (positif), katalase negatif sedangkan 5 isolat berbentuk bulat, berwarna ungu dan katalase positif. Hasil zona hambat yang dihasilkan dari isolat BAL asal rusip terhadap bakteri E. coli antara 2,85 mm - 3,43 mm dan S. aureus antara 2,55 mm - 4,10 mm. Berdasarkan hasil penelitian diperoleh kesimpulan bahwa perbedaan konsentrasi gula aren cair pada pembuatan rusip tidak berpengaruh nyata terhadap hasil zona hambat bakteri E. coli dan S. aureus.}, issn = {2685-3701}, pages = {54--63} doi = {10.14710/jitpi.2020.8089}, url = {https://ejournal2.undip.ac.id/index.php/jitpi/article/view/8089} }
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