DOI: https://doi.org/10.14710/jbtr.v6i1.6135

Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia

Jenderal Soedirman University, Indonesia

Received: 21 Oct 2019; Revised: 27 Feb 2020; Accepted: 31 Mar 2020; Available online: 30 Apr 2020; Published: 30 Apr 2020.
Open Access Copyright (c) 2020 Journal of Biomedicine and Translational Research

Citation Format:
Abstract

Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen.

Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia.

Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district.  Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method.

Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination,  one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene.

Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Instrument
Judul Table dan Gambar
Subject
Type Research Instrument
  Download (13KB)    Indexing metadata
 Research Instrument
Gambar
Subject
Type Research Instrument
  Download (252KB)    Indexing metadata
 Research Instrument
Tabel
Subject
Type Research Instrument
  Download (13KB)    Indexing metadata
Keywords: Antibiotic resistance; Microtiter plate biofilm assay; MRSA; MSSA; Nasal swab
Funding: Kementerian Riset Teknologi dan Pendidikan Tinggi Republik Indonesia

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
Randomized Controlled Trial of Tranexamic Acid’s Effect on Bleeding Length: A Study on DMPA Users with Abnormal Uterine Bleeding Who Receive Low-Dose Oral Contraceptive Pill Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein) Association of Growth Velocity with Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3 Levels in Children with a Vegan Diet Unraveling of Diagnosis Odyssey in A Girl with Primary Amenorrhea: A case report Fragile X Syndrome and Targeted Treatments More recent articles
  1. Becker, K., Heilmann, C., & Peters, G. (2014). Coagulase-negative staphylococci in the Endophtalmitis Vitrectomy Study. Clinical Microbiology Reviews, 27(4), 870–926. https://doi.org/10.1128/CMR.00109-13
  2. Chen, C. J., & Huang, Y. C. (2014). New epidemiology of Staphylococcus aureus infection in Asia. (Themed Section: New epidemiology of Staphylococcus aureus infections.). Clinical Microbiology and Infection
  3. Darwish, S. F., & Asfour, H. A. E. (2013). Investigation of biofilm forming ability in staphylococci causing bovine mastitis using phenotypic and genotypic assays. The Scientific World Journal. https://doi.org/10.1155/2013/378492
  4. Foster, T. J. (2005). Immune evasion by staphylococci. Nature Reviews Microbiology. https://doi.org/10.1038/nrmicro1289
  5. Golia S, Hittinahalli V, Kamath AS, N. A. (2013). A study of nasal carriage of MRSA among the health care workers of a tertiary care hospital, Bangalore. International Journal of Basic Applied Medical Science, 3, 3–7
  6. Hassan, A., Usman, J., Kaleem, F., Omair, M., Khalid, A., & Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. Brazilian Journal of Infectious Diseases. https://doi.org/10.1590/S1413-86702011000400002
  7. Høiby, N., Bjarnsholt, T., Givskov, M., Molin, S., & Ciofu, O. (2010). Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents. https://doi.org/10.1016/j.ijantimicag.2009.12.011
  8. John, N. P., & Murugan, S. (2014). Biofilm Formation by Methicillin Resistant Staphylococcus aureus and their Antibiotic Susceptibility Pattern: An in vitro Study. Current Research in Bacteriology, Vol. 7, pp. 1–11. https://doi.org/10.3923/crb.2014.1.11
  9. Kwiecinski, J., Kahlmeter, G., & Jin, T. (2015). Biofilm formation by staphylococcus aureus isolates from skin and soft tissue infections. Current Microbiology. https://doi.org/10.1007/s00284-014-0770-x
  10. Lin, M. H., Shu, J. C., Lin, L. P., Chong, K. Y., Cheng, Y. W., Du, J. F., & Liu, S. T. (2015). Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. PLoS ONE. https://doi.org/10.1371/journal.pone.0124216
  11. Martínez-Aguilar, G., Hammerman, W. A., Mason, E. O., & Kaplan, S. L. (2003). Clindamycin treatment of invasive infections caused by community-acquired, methicillin-resistant and methicillin-susceptible Staphylococcus aureus in children. Pediatric Infectious Disease Journal. https://doi.org/10.1097/00006454-200307000-00006
  12. Oliveira, A., & Cunha, M. D. L. R. S. (2010). Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci. BMC Research Notes. https://doi.org/10.1186/1756-0500-3-260
  13. Otsuka, T., Yoshida, K., Komiyama, K., Ishikawa, Y., Zaraket, H., & Okazaki, M. (2011). Prevalence of methicillin-resistant Staphylococcus aureus among children in a region with controlled antimicrobial use. Japanese Journal of Infectious Diseases
  14. Piechota, M., Kot, B., Frankowska-Maciejewska, A., Gruzewska, A., & Woźniak-Kosek, A. (2018). Biofilm Formation by Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains from Hospitalized Patients in Poland. BioMed Research International. https://doi.org/10.1155/2018/4657396
  15. Piper Jenks, N., Pardos de la Gandara, M., D’Orazio, B. M., Correa da Rosa, J., Kost, R. G., Khalida, C., … Tobin, J. N. (2016). Differences in prevalence of community-associated MRSA and MSSA among U.S. and non-U.S. born populations in six New York Community Health Centers. Travel Medicine and Infectious Disease. https://doi.org/10.1016/j.tmaid.2016.10.003
  16. Prescott LM, Harley JP, K. DA. (2017). Microbiology 10th Edition. Boston: McGraw-Hill
  17. Rongpharpi, S. R., Hazarika, N. K., & Kalita, H. (2013). The prevalence of nasal carriage of Staphylococcus aureus among healthcare workers at a tertiary care hospital in Assam with special reference to MRSA. Journal of Clinical and Diagnostic Research. https://doi.org/10.7860/JCDR/2013/4320.2741
  18. Ryan, K. J., & Ray, C. G. (2011). Sherris Microbiología Médica. In Sherris medical microbiology. https://doi.org/10.1017/CBO9781107415324.004
  19. Salman MK, Ashraf MS, Sumaira Iftikhar, M. A. R. B. (2018). Frequency of nasal carriage of Staphylococcus aureus among health care workers at a Tertiary Care Hospital. Pakistan Journal of Medical Science, 34(5), 1181–1184
  20. Thilakavathy, P., Vasantha Priyan, R. M., Jagatheeswari, P. A. T., Charles, J., Dhanalakshmi, V., Lallitha, S., … Divya, B. (2015). Evaluation of Ica gene in comparison with phenotypic methods for detection of biofilm production by coagulase negative staphylococci in a tertiary care hospital. Journal of Clinical and Diagnostic Research. https://doi.org/10.7860/JCDR/2015/11725.6371
  21. Tiewsoh, J. A., & Dias, M. (2017). Screening of methicillin-resistant Staphylococcus aureus in healthcare workers and students and its susceptibility to mupirocin in a tertiary care teaching hospital in South India. Journal of Laboratory Physicians. https://doi.org/10.4103/0974-2727.214262
  22. Weinstein MP, Patel JB, Bobenchik AM, Campeau S, Cullen SK, Galas MF, G. H. (2019). Performance Standards for Antimicrobial Susceptibility Testing Performance Standards for Antimicrobial Susceptibility Testing. CLSI, Supplement M100
  23. WHO. (2014). Antimicrobial resistance. Global report on surveillance. World Health Organization. https://doi.org/10.1007/s13312-014-0374-3
  24. Zhao, C., Liu, Y., Zhao, M., Liu, Y., Yu, Y., Chen, H., … Wang, H. (2012). Characterization of community acquired staphylococcus aureus associated with skin and soft tissue infection in beijing: High prevalence of PVL + ST398. PLoS ONE. https://doi.org/10.1371/journal.pone.0038577

Last update:

No citation recorded.

Last update:

No citation recorded.