The Effect of Bacillus subtilis on Bioethanol Production from Ambon  Banana (Musa paradisiaca var. sapientum Linn) Peels by Using Fermentation Process

Gaudentius Bilyartinus; Anggun Puspitarini Siswanto

doi:10.14710/jvsar.v3i2.11081

DOI: https://doi.org/10.14710/jvsar.v3i2.11081

The Effect of Bacillus subtilis on Bioethanol Production from Ambon Banana (Musa paradisiaca var. sapientum Linn) Peels by Using Fermentation Process

Gaudentius Bilyartinus - Department of Industrial Chemical Engineering, Vocational School, Diponegoro University, Indonesia

*Anggun Puspitarini Siswanto - Department of Industrial Chemical Engineering, Vocational School, Diponegoro University, Indonesia

BibTex Citation Data :

@article{JVSAR11081,
    author = {Gaudentius Bilyartinus and Anggun Siswanto},
    title = {The Effect of Bacillus subtilis on Bioethanol Production from Ambon  Banana (Musa paradisiaca var. sapientum Linn) Peels by Using Fermentation Process},
    journal = {Journal of Vocational Studies on Applied Research},
  volume = {3},
    number = {2},
    year = {2021},
    keywords = {Ambon Banana Peels;Bacillus subtilis;Bioethanol;Fermentation;pH},
    abstract = {    Abstract -       The breakthrough to optimize Indonesia's energy is by utilizing abundant renewable energy sources. Its geographic location has the potential to become a country with the largest renewable energy source in the world. The potential energy that comes from vegetables is called bioethanol. Bioethanol is an organic fuel produced by bioethanol fermentation. It can be an alternative material for making sanitary products amid the increasing spread of      COVID-19 in Indonesia.      So the authors try to innovate how to use Ambon Banana (Musa paradisiaca var. sapientum Linn) peels to become bioethanol. This research aimed to investigate the effect of B    acillus     subtilis in the fermentation of bioethanol production from Ambon banana peels. The methods used were pretreatment, hydrolysis, fermentation, and distillation. Bacillus subtilis and Saccharomyces cerevisiae ratio (10: 5 and 5: 5) use for obtaining high ethanol yields, as well as variations in pH 2 and 6 in the fermentation starter. The variable were designed by using Factorial Design. The result show    s     the yeast ratio of B. subtilis and S. cerevisiae (10: 5) resulted the highest concentration of bioethanol (6%) in 6 days. In acidic conditions (pH 2), the bacteria don’t grow optimally. The higher concentration of bioethanol (6%) was reached  in pH 6.    The research supports the Government Program Reducing the dependency of fossil fuels and innovation to produce sanitary product from bioethanol.       Abstract -       The breakthrough to optimize Indonesia's energy is by utilizing abundant renewable energy sources. Its geographic location has the potential to become a country with the largest renewable energy source in the world. The potential energy that comes from vegetables is called bioethanol. Bioethanol is an organic fuel produced by bioethanol fermentation. It can be an alternative material for making sanitary products amid the increasing spread of      COVID-19 in Indonesia.      So the authors try to innovate how to use Ambon Banana (Musa paradisiaca var. sapientum Linn) peels to become bioethanol. This research aimed to investigate the effect of B    acillus     subtilis in the fermentation of bioethanol production from Ambon banana peels. The methods used were pretreatment, hydrolysis, fermentation, and distillation. Bacillus subtilis and Saccharomyces cerevisiae ratio (10: 5 and 5: 5) use for obtaining high ethanol yields, as well as variations in pH 2 and 6 in the fermentation starter. The variable were designed by using Factorial Design. The result show    s     the yeast ratio of B. subtilis and S. cerevisiae (10: 5) resulted the highest concentration of bioethanol (6%) in 6 days. In acidic conditions (pH 2), the bacteria don’t grow optimally. The higher concentration of bioethanol (6%) was reached    in pH 6.    The research supports the Government Program Reducing the dependency of fossil fuels and innovation to produce sanitary product from bioethanol.   },
   issn = {2684-8090},   pages = {26--30}  doi = {10.14710/jvsar.v3i2.11081},
    url = {https://ejournal2.undip.ac.id/index.php/jvsar/article/view/11081}
}

Citation Format:

Abstract

Abstract -The breakthrough to optimize Indonesia's energy is by utilizing abundant renewable energy sources. Its geographic location has the potential to become a country with the largest renewable energy source in the world. The potential energy that comes from vegetables is called bioethanol. Bioethanol is an organic fuel produced by bioethanol fermentation. It can be an alternative material for making sanitary products amid the increasing spread ofCOVID-19 in Indonesia.So the authors try to innovate how to use Ambon Banana (Musa paradisiaca var. sapientum Linn) peels to become bioethanol. This research aimed to investigate the effect of Bacillus subtilis in the fermentation of bioethanol production from Ambon banana peels. The methods used were pretreatment, hydrolysis, fermentation, and distillation. Bacillus subtilis and Saccharomyces cerevisiae ratio (10: 5 and 5: 5) use for obtaining high ethanol yields, as well as variations in pH 2 and 6 in the fermentation starter. The variable were designed by using Factorial Design. The result shows the yeast ratio of B. subtilis and S. cerevisiae (10: 5) resulted the highest concentration of bioethanol (6%) in 6 days. In acidic conditions (pH 2), the bacteria don’t grow optimally. The higher concentration of bioethanol (6%) was reached in pH 6.The research supports the Government Program Reducing the dependency of fossil fuels and innovation to produce sanitary product from bioethanol.

Fulltext View|Download

Keywords: Ambon Banana Peels;Bacillus subtilis;Bioethanol;Fermentation;pH

Article Metrics:

Article Info

Section: Articles

Language : EN

In Volume 3, Issue 2, Year 2021 (October 2021)

Recent articles

Temperature Optimization and Addition of Aloe Vera Gel as Antibacteria In Coconut Oil-Based Liquid Soap The Effectiveness of Biosorbent from Chicken’s Egg Shell and Durian Peel towards the Quality of Biodiesel Product from Waste Cooking Oil Optimization of clove flower oil extraction (Syzgium aromaticum L.) With Factorial Design Method More recent articles

International Energy Agency, Data & Statistics - IEA, 2020. https://www.iea.org (accessed Apr. 19, 2021)
H. N. Saturwa, S. Suharno, and A. A. Ahmad, The impact of Covid-19 pandemic on MSMEs, J. Ekon. dan Bisnis, vol. 24, no. 1, pp. 65–82, 2021, doi: 10.24914/jeb.v24i1.3905
H. Herliati, S. Sefaniyah, and A. Indri, Pemanfaatan limbah kulit pisang sebagai Bahan Baku pembuatan Bioetanol, J. Teknol., vol. 6, no. 1, pp. 1–10, 2019, doi: 10.31479/jtek.v6i1.1
U. Salsabila, D. Mardiana, and E. Indahyanti, Kinetika Reaksi Fermentasi Glukosa Hasil Hidrolisis Pati Biji Durian Menjadi Etanol, Kim. Student J., vol. 2, no. 1, pp. 331–336, 2013
A. Gebregergs, M. Gebresemati, and O. Sahu, Industrial ethanol from banana peels for developing countries: Response surface methodology, Pacific Sci. Rev. A Nat. Sci. Eng., vol. 18, no. 1, pp. 22–29, 2016, doi: 10.1016/j.psra.2016.06.002
C. Laluce, A. C. G. Schenberg, J. C. M. Gallardo, L. F. C. Coradello, and S. R. Pombeiro-Sponchiado, Advances and developments in strategies to improve strains of Saccharomyces cerevisiae and processes to obtain the lignocellulosic ethanol - A review, Appl. Biochem. Biotechnol., vol. 166, no. 8, pp. 1908–1926, Apr. 2012, doi: 10.1007/s12010-012-9619-6
N. M. S. Sanjiwani, W. S. Rita, Swantara, and dan I. M. Dira, Pembuatan bioetanol dari campuran limbah nasi dan kulit pisang, Cakra Kim. (Indonesian E-Journal Appl. Chem., vol. 6, pp. 145–152, 2018
R. P. Mahyudin, Kajian Permasalahan Pengelolaan Sampah Dan Dampak Lingkungan Di Tpa (Tempat Pemrosesan Akhir), Jukung (Jurnal Tek. Lingkungan), vol. 3, no. 1, pp. 66–74, 2017, doi: 10.20527/jukung.v3i1.3201
O. W. Ermawati, S. Wahyuni, and Sr. Rejeki, Kajian Pemanfaatan Limbah Kulit Pisang Raja paradisiaca var Raja) Dalam Pembuatan Es Krim, J. Sains dan Teknol. Pangan, vol. 1, no. 1, pp. 67–72, 2016
D. R. Setiawati, A. R. Sinaga, and T. K. Dewi, Proses Pembuatan Bioetanol Dari Kulit Pisang Kepok, J. Tek. Kim., vol. 19, no. 1, pp. 9–15, 2013, doi: 10.1186/1471-2377-14-103
B. A. Anhwange, T. J. Ugye, and T. D. Nyiaatagher, Chemical composition of Musa sapientum (Banana) peels, Electron. J. Environ. Agric. Food Chem., vol. 8, no. 6, pp. 437–442, 2009
H. F. Hassan, U. F. , Hassan, O. A. , Usher, and N. N. , Ibrahim, A. B. and Tabe, Exploring the Potentials of Banana (Musa Sapietum) Peels in Feed Formulation, Int. J. Adv. Res. Chem. Sci., vol. 5, no. 5, pp. 10–14, 2018, doi: 10.20431/2349-0403.0505003
P. M. Cruz, A. L. Ibáñez, O. A. M. Hermosillo, and H. C. R. Saad, Use of Probiotics in Aquaculture, ISRN Microbiol., vol. 2012, pp. 1–13, 2012, doi: 10.5402/2012/916845
S. Romero, E. Merino, F. Bolı, G. Gosset, and A. Martinez, Metabolic Engineering of Bacillus subtilis for Ethanol Production : Lactate Dehydrogenase Plays a Key Role in Fermentative Metabolism, vol. 73, no. 16, pp. 5190–5198, 2007, doi: 10.1128/AEM.00625-07
A. Ahmad, T. Sathivelu, S. Ravichandran, K. Rajandrin, M. S. R. M. Roshd, and Z. Yusoff, Identification best type of banana peel (glucose contain) to produce bioethanol for the development of biofuel (E85), Proc. - 2015 Innov. Commer. Med. Electron. Technol. Conf. ICMET 2015, no. December, pp. 30–34, 2016, doi: 10.1109/ICMETC.2015.7449568
D. Wakano, E. Samson, and L. D. Tetelepta, Pemanfaatan Limbah Kulit Pisang Sebagai Bahan Olahan Kripik Dan Kue Donat Di Desa Batu Merah Kota Ambon, J. Biol. Sci. Educ., vol. 5, no. 2, pp. 9–22, 2016
H. D. T. Le and V. V. M. Le, Effects of initial pH value of the medium on the alcoholic fermentation performance of Saccharomyces cerevisiae cells immobilized on nipa leaf sheath pieces, Songklanakarin J. Sci. Technol., vol. 36, no. 6, pp. 663–667, 2014
D. Rutz and R. Janssen, 2008. Biofuel Technology Handbook. WIP Renewable Energy, München
I. G. B. W. Kusuma, Pengolahan Sampah Organik Menjadi Etanol Dan Pengujian Sifat Fisika Biogasoline, Semin. Nas. Tah. Tek. Mesin ke-9 Palembang, 13-15 Oktober 2010, pp. 13–15, 2010
I. M. A. S. Wijaya, I. G. K. A. Arthawan, and A. N. Sari, Potensi Nira Kelapa Sebagai Bahan Baku Bioetanol, J. Bumi Lestari, vol. 12, no. 1, pp. 85–92, 2012

Last update:

No citation recorded.

Last update:

No citation recorded.

Journal of Vocational Studies on Applied Research are published under the terms of the Creative Commons Attribution-ShareAlike. Authors retain the copyright to their work. Users may read, copy and distribute the work in any medium provided the authors and the journal are appropriately credited.

Below you may find the full text of the license signed by authors.

LICENSE TO PUBLISH

1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution-ShareAlike license as currently displayed on http://creativecommons.org/licenses/by-sa/4.0/. Without prejudice to the foregoing, the author hereby grants Journal of Vocational Studies on Applied Research the exclusive license for commercial use of the article according to section 2 below, and sections 4 through 9 below, throughout the world, in any form, in any language, for the full term of copyright, effective upon acceptance for publication.

2. Author’s Warranties
The author warrants that the article is original, written by stated author/s, has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author/s.

3. User Rights
Under the Creative Commons Attribution-ShareAlike license, the author(s) and users are free to share (copy, distribute and transmit the contribution).

4. Rights of Authors
Authors retain the following rights:

copyright, and other proprietary rights relating to the article, such as patent rights,
the right to use the substance of the article in future own works, including lectures and books,
the right to reproduce the article for own purposes, provided the copies are not offered for sale,
the right to self-archive the article.

5. Co-Authorship
If the article was prepared jointly with other authors, the signatory of this form warrants that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.

6. Termination
This agreement can be terminated by the author or Journal of Vocational Studies on Applied Research upon two months’ notice where the other party has materially breached this agreement and failed to remedy such breach within a month of being given the terminating party’s notice requesting such breach to be remedied. No breach or violation of this agreement will cause this agreement or any license granted in it to terminate automatically or affect the definition of Journal of Vocational Studies on Applied Research.

7. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by Journal of Vocational Studies on Applied Research or its sublicensee.

8. Miscellaneous
Journal of Vocational Studies on Applied Research will publish the article (or have it published) in the Journal, if the article’s editorial process is successfully completed and Journal of Vocational Studies on Applied Research or its sublicensee has become obligated to have the article published. Journal of Vocational Studies on Applied Research may conform the article to a style of punctuation, spelling, capitalization and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers. Journal of Vocational Studies on Applied Research will be allowed to sublicense the rights that are licensed to it under this agreement.