DOI: https://doi.org/10.14710/jvsar.v3i2.11081

The Effect of Bacillus subtilis on Bioethanol Production from Ambon Banana (Musa paradisiaca var. sapientum Linn) Peels by Using Fermentation Process

Department of Industrial Chemical Engineering, Vocational School, Diponegoro University, Indonesia

Received: 28 May 2021; Published: 31 Oct 2021.
Open Access Copyright (c) 2021 by Authors, Published by Vocational College of Universitas Diponegoro under http://creativecommons.org/licenses/by-sa/4.0.

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Abstract

The breakthrough to optimize Indonesia's energy is by utilizing abundant renewable energy sources. Its geographic location has the potential to become a country with the largest renewable energy source in the world. The potential energy that comes from vegetables is called bioethanol. Bioethanol is an organic fuel produced by bioethanol fermentation. It can be an alternative material for making sanitary products amid the increasing spread ofCOVID-19 in Indonesia.So the authors try to innovate how to use Ambon Banana (Musa paradisiaca var. sapientum Linn) peels to become bioethanol. This research aimed to investigate the effect of Bacillus subtilis in the fermentation of bioethanol production from Ambon banana peels. The methods used were pretreatment, hydrolysis, fermentation, and distillation. Bacillus subtilis and Saccharomyces cerevisiae ratio (10: 5 and 5: 5) use for obtaining high ethanol yields, as well as variations in pH 2 and 6 in the fermentation starter. The variable were designed by using Factorial Design. The result shows the yeast ratio of B. subtilis and S. cerevisiae (10: 5) resulted the highest concentration of bioethanol (6%) in 6 days. In acidic conditions (pH 2), the bacteria don’t grow optimally. The higher concentration of bioethanol (6%) was reached  in pH 6.The research supports the Government Program Reducing the dependency of fossil fuels and innovation to produce sanitary product from bioethanol.

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Keywords: Ambon Banana Peels; Bacillus subtilis; Bioethanol; Fermentation; pH

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Section: Original Research Articles
Language : EN
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  1. International Energy Agency, Data & Statistics - IEA, 2020. https://www.iea.org (accessed Apr. 19, 2021)
  2. H. N. Saturwa, S. Suharno, and A. A. Ahmad, The impact of Covid-19 pandemic on MSMEs, J. Ekon. dan Bisnis, vol. 24, no. 1, pp. 65–82, 2021, doi: 10.24914/jeb.v24i1.3905
  3. H. Herliati, S. Sefaniyah, and A. Indri, Pemanfaatan limbah kulit pisang sebagai Bahan Baku pembuatan Bioetanol, J. Teknol., vol. 6, no. 1, pp. 1–10, 2019, doi: 10.31479/jtek.v6i1.1
  4. U. Salsabila, D. Mardiana, and E. Indahyanti, Kinetika Reaksi Fermentasi Glukosa Hasil Hidrolisis Pati Biji Durian Menjadi Etanol, Kim. Student J., vol. 2, no. 1, pp. 331–336, 2013
  5. A. Gebregergs, M. Gebresemati, and O. Sahu, Industrial ethanol from banana peels for developing countries: Response surface methodology, Pacific Sci. Rev. A Nat. Sci. Eng., vol. 18, no. 1, pp. 22–29, 2016, doi: 10.1016/j.psra.2016.06.002
  6. C. Laluce, A. C. G. Schenberg, J. C. M. Gallardo, L. F. C. Coradello, and S. R. Pombeiro-Sponchiado, Advances and developments in strategies to improve strains of Saccharomyces cerevisiae and processes to obtain the lignocellulosic ethanol - A review, Appl. Biochem. Biotechnol., vol. 166, no. 8, pp. 1908–1926, Apr. 2012, doi: 10.1007/s12010-012-9619-6
  7. N. M. S. Sanjiwani, W. S. Rita, Swantara, and dan I. M. Dira, Pembuatan bioetanol dari campuran limbah nasi dan kulit pisang, Cakra Kim. (Indonesian E-Journal Appl. Chem., vol. 6, pp. 145–152, 2018
  8. R. P. Mahyudin, Kajian Permasalahan Pengelolaan Sampah Dan Dampak Lingkungan Di Tpa (Tempat Pemrosesan Akhir), Jukung (Jurnal Tek. Lingkungan), vol. 3, no. 1, pp. 66–74, 2017, doi: 10.20527/jukung.v3i1.3201
  9. O. W. Ermawati, S. Wahyuni, and Sr. Rejeki, Kajian Pemanfaatan Limbah Kulit Pisang Raja paradisiaca var Raja) Dalam Pembuatan Es Krim, J. Sains dan Teknol. Pangan, vol. 1, no. 1, pp. 67–72, 2016
  10. D. R. Setiawati, A. R. Sinaga, and T. K. Dewi, Proses Pembuatan Bioetanol Dari Kulit Pisang Kepok, J. Tek. Kim., vol. 19, no. 1, pp. 9–15, 2013, doi: 10.1186/1471-2377-14-103
  11. B. A. Anhwange, T. J. Ugye, and T. D. Nyiaatagher, Chemical composition of Musa sapientum (Banana) peels, Electron. J. Environ. Agric. Food Chem., vol. 8, no. 6, pp. 437–442, 2009
  12. H. F. Hassan, U. F. , Hassan, O. A. , Usher, and N. N. , Ibrahim, A. B. and Tabe, Exploring the Potentials of Banana (Musa Sapietum) Peels in Feed Formulation, Int. J. Adv. Res. Chem. Sci., vol. 5, no. 5, pp. 10–14, 2018, doi: 10.20431/2349-0403.0505003
  13. P. M. Cruz, A. L. Ibáñez, O. A. M. Hermosillo, and H. C. R. Saad, Use of Probiotics in Aquaculture, ISRN Microbiol., vol. 2012, pp. 1–13, 2012, doi: 10.5402/2012/916845
  14. S. Romero, E. Merino, F. Bolı, G. Gosset, and A. Martinez, Metabolic Engineering of Bacillus subtilis for Ethanol Production : Lactate Dehydrogenase Plays a Key Role in Fermentative Metabolism, vol. 73, no. 16, pp. 5190–5198, 2007, doi: 10.1128/AEM.00625-07
  15. A. Ahmad, T. Sathivelu, S. Ravichandran, K. Rajandrin, M. S. R. M. Roshd, and Z. Yusoff, Identification best type of banana peel (glucose contain) to produce bioethanol for the development of biofuel (E85), Proc. - 2015 Innov. Commer. Med. Electron. Technol. Conf. ICMET 2015, no. December, pp. 30–34, 2016, doi: 10.1109/ICMETC.2015.7449568
  16. D. Wakano, E. Samson, and L. D. Tetelepta, Pemanfaatan Limbah Kulit Pisang Sebagai Bahan Olahan Kripik Dan Kue Donat Di Desa Batu Merah Kota Ambon, J. Biol. Sci. Educ., vol. 5, no. 2, pp. 9–22, 2016
  17. H. D. T. Le and V. V. M. Le, Effects of initial pH value of the medium on the alcoholic fermentation performance of Saccharomyces cerevisiae cells immobilized on nipa leaf sheath pieces, Songklanakarin J. Sci. Technol., vol. 36, no. 6, pp. 663–667, 2014
  18. D. Rutz and R. Janssen, 2008. Biofuel Technology Handbook. WIP Renewable Energy, München
  19. I. G. B. W. Kusuma, Pengolahan Sampah Organik Menjadi Etanol Dan Pengujian Sifat Fisika Biogasoline, Semin. Nas. Tah. Tek. Mesin ke-9 Palembang, 13-15 Oktober 2010, pp. 13–15, 2010
  20. I. M. A. S. Wijaya, I. G. K. A. Arthawan, and A. N. Sari, Potensi Nira Kelapa Sebagai Bahan Baku Bioetanol, J. Bumi Lestari, vol. 12, no. 1, pp. 85–92, 2012

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