DOI: https://doi.org/10.14710/jbtr.v10i2.22207

The Effect of Administration of Sapodilla Leaf Extract Cream (Manilkara Zapota (L.) P. Royen) On the Expression of PDGF And Il-10

1Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung,, Indonesia

2Department of Postgraduate Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung, Indonesia

Received: 4 Mar 2024; Accepted: 27 Aug 2024; Available online: 30 Aug 2024; Published: 30 Aug 2024.
Open Access Copyright (c) 2024 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Background: Sunburn is an acute inflammatory skin condition caused by exposure to UV rays. Excessive exposure increases the production of  ROS which, if accumulated, can lyse growth factors, one of which is PDGF and also form IL-10 immune suppression. This condition can be influenced by providing antioxidants and anti-inflammatories such as those contained in sapodilla leaf extract (Manilkara zapota (L.) P. Royen) which has many benefits such as anti-inflammatory, anti-pyretic, anti-tumor, antioxidant, anti-microbial, anti- diabetic, anti-lipid and anti-aging. In previous research, the polyphenol content in Sapodilla leaves was a potential source of inhibiting ROS. However, until now the role of Sapodilla leaf extract on UVB burns has not been studied.

Objective: The aim of this research is to determine the effect of administering Sapodilla leaf extract cream on PDFG and IL-10 in Wistar rats that experienced burns due to exposure to UVB.

Method: Experimental research with a post test only control group design approach. This research used 24 Wistar rats exposed to UVB rays which were divided into 4 groups (normal control, control with cream, 25% Sapodilla leaf extract cream, and 50% Sapodilla leaf extract cream). The ELISA (Enzyme-Linked Immunosorbent Assay) method was used to analyze PDGF and IL-10 levels in skin tissue.

Results: The highest ratio of PDGF levels was found in (K3) 2.915 ± 0.368. The results of the one way Anova analysis had a p value of 0.024 (p<0.05) which stated that there were significant differences between treatment groups. In IL-10 levels there was an increase in K3 255.9 ± 35,563. In IL-10, the results of one way Anova analysis had a p value of 0.240 (p>0.05), which stated that there were no significant differences between treatment groups.

Conclusion: Administration of sapodilla leaf extract cream at a dose of 25% had a significant effect on increasing PDGF levels and a slight increase in IL-10 in mice that experienced burns due to exposure to UVB.

Fulltext View|Download
Keywords: Manilkara zapota (L.) P. Royen, PDFG, IL-10, ELISA Method

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
Psychotherapy Supportive Practice For Borderline Personality Disorder (A Clinical Module Development) The Effect of Sambiloto (Andrographis paniculata) Leaf Extract in Combination with Phosphomycin on The Germ Number, Urine Leukocyte Esterase, and Urine Procalcitonin Levels in Wistar Rats (Rattus norvegicus) Urinary Tract Infection Model The Effect of Administration of Sapodilla Leaf Extract Cream (Manilkara Zapota (L.) P. Royen) On the Expression of PDGF And Il-10 More recent articles
  1. Lee LY, Liu SX. Pathogenesis of Photoaging in Human Dermal Fibroblasts. Int J Dermatology Venereol. 2021;0(August 2019):37–42
  2. doi: 10.1097/JD9.0000000000000068
  3. Mahobiya P. Protection against Ultraviolet B Radiation-Induced Oxidative Damage by Antioxidants on Liver of Female Wistar rat. 2020;(March)
  4. doi: 10.13140/RG.2.2.27371.98081
  5. González Maglio DH, Paz ML, Leoni J. Sunlight Effects on Immune System: Is There Something Else in addition to UV-Induced Immunosuppression? Biomed Res Int. 2016;2016
  6. doi: 10.1155/2016/1934518
  7. Mohd Tamsir N, Mohd Esa N, Omar SNC, Shafie NH. Manilkara zapota (L.) P. Royen: Potential source of natural antioxidants. Malaysian J Med Heal Sci. 2020;16(8):196–204
  8. Ganguly A, Mahmud Z Al, Uddin MMN, Rahman SMA. In-vivo anti-inflammatory and anti-pyretic activities of Manilkara zapota leaves in albino Wistar rats. Asian Pacific J Trop Dis. 2013 Aug 1;3(4):301–7
  9. doi: 10.1016/S2222-1808(13)60073-0
  10. Holman DM, Ding H, Guy GP, Watson M, Hartman AM, Perna FM. Prevalence of sun protection use and sunburn and association of demographic and behaviorial characteristics with sunburn among US adults. JAMA Dermatology. 2018;154(5):561–8
  11. doi: 10.1001/jamadermatol.2018.0028
  12. Gaddameedhi S, Selby CP, Kemp MG, Ye R, Sancar A. The Circadian Clock Controls Sunburn Apoptosis and Erythema in Mouse Skin. J Invest Dermatol [Internet]. 2015;135(4):1119–27. Available from: http://dx.doi.org/10.1038/jid.2014.508
  13. doi.org/10.1038/jid.2014.508
  14. Jordaniel Setiabudi, Made Wardhana, I Gusti Ayu Agung Elis Indira NMDP. Profil Pra Kanker dan Kanker Kulit RSUP Sanglah Periode 2015 - 2018. J Med Udayana. 2021;10(3):83–9
  15. doi: 10.24843.MU.2021.V10.i3.P13
  16. Ansary TM, Hossain MR, Kamiya K, Komine M, Ohtsuki M. Inflammatory molecules associated with ultraviolet radiation‐mediated skin aging. Int J Mol Sci. 2021;22(8)
  17. doi.org/10.3390/ijms22083974
  18. Yang X, Zhan P, Wang X, Zhang Q, Zhang Y, Fan H, et al. Polydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion. PLoS One [Internet]. 2020;15(9 September):1–18
  19. doi: 10.1371/journal.pone.0239366
  20. Agrawal S, Ganguly S, Hajian P, Cao JN, Agrawal A. PDGF upregulates CLEC-2 to induce T regulatory cells. Oncotarget. 2015;6(30):28621–32
  21. doi: 10.18632/oncotarget.5765
  22. Saric S, Sivamani RK. Polyphenols and sunburn. Int J Mol Sci. 2016;17(9):1–22
  23. doi: 10.3390/ijms17091521
  24. Chunhakant S, Chaicharoenpong C. Antityrosinase, Antioxidant, and Cytotoxic Activities of Phytochemical Constituents from Manilkara zapota L. Bark. Molecules. 2019;24(15):1–18
  25. doi: 10.3390/molecules24152798
  26. Pientaweeratch S, Panapisal V, Tansirikongkol A. Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications. Pharm Biol. 2016 Sep 1;54(9):1865–72
  27. doi.org/10.3109/13880209.2015.1133658
  28. Octaviani M, Syafrina S. Uji Aktivitas Antibakteri Ekstrak Etanol Daun dan Kulit Batang Sawo (Manilkara zapota (L.) Van Royen). J Ilmu Kefarmasian Indones. 2018;16(2):131
  29. Puspitasari AD, Yuita NE, Sumantri S. Krim Antioksidan ektrak Etanol Daun Kopi Arabika(Coffea Arabica). J Ilm Teknosains. 2017;3(2)
  30. doi: 10.26877/jitek.v3i2.1884
  31. Prosedur Pemeriksaan ELISA - Publikasi Jurnal Ilmiah News - HM Publisher (cattleyapublicationservices)
  32. Nobile V, Zanoletti V, Manzoni V, Romagnoli S, Cestone E. Soothing Effect of a Cosmetic Product on Skin Discomforts Induced by a Chemical Irritant (Capsaicin) and UV-Radiation, and after Mosquito Bites and Sunburn in a Real-World Setting. Cosmetics. 2022;9(6)
  33. doi: 10.3390/cosmetics9060130
  34. Nakamura M, Haarmann-Stemmann T, Krutmann J. COX inhibition enhances inflammatory immune cell infiltration in UV-irradiated human skin: Implications for the treatment of sunburn. Exp Dermatol. 2015;24(10):734–5
  35. doi: 10.1111/exd.12783
  36. Gallo RL, Bernard JJ. Innate immune sensors stimulate inflammatory and immunosuppressive responses to UVB radiation. J Invest Dermatol. 2014;134(6):1508–11
  37. doi: 10.1038/jid.2014.32
  38. Anbualakan K, Tajul Urus NQ, Makpol S, Jamil A, Mohd Ramli ES, Md Pauzi SH, et al. A Scoping Review on the Effects of Carotenoids and Flavonoids on Skin Damage Due to Ultraviolet Radiation. Nutrients. 2023;15(1):1–17
  39. doi: 10.3390/nu15010092
  40. Los UMDECDE. Clitorea Ternatea Flower Extract Induces Platelet Derived Growth factor (PDGF) and GPx Gene Overexpression In Ultraviolet (UV)B Irradiation- Induced Collagen Loss. Med Glas. 2023;20(1):15
  41. doi: 10.17392/1530-22
  42. Lopes DM, Mcmahon SB. Ultraviolet Radiation on the Skin: A Painful Experience? CNS Neurosci Ther. 2016;22(2):118–26
  43. doi: 10.1111/cns.12444
  44. Konuku K, Karri KC, Gopalakrishnan VK, Hagos Z, Kebede H, Naidu TK, et al. Anti-Inflammatory Activity of Manilkara Zapota Leaf Extract. Int J Curr Pharm Res. 2017;9(4):130
  45. doi.org/10.22159/ijcpr.2017v9i4.20977
  46. S. S. Phytochemical Screening and In Vitro Corneal Wound Healing Activities of the Leaves of Manilkara Zapota In Newer Herbal Drug Development. World J Pharm Res. 2017;6(9):639–47
  47. doi: 10.20959/wjpr20179-9224
  48. Putranti IO, Sistina Y, Tengah J, Fisiologi D, Biologi F, Jenderal U, et al. Tinjauan Pustaka Fotobiologi Ultraviolet Pada Jaringan Kulit. 2023;13(2)
  49. doi: 10.20884/1.mandala.2023.16.1.8379

Last update:

No citation recorded.

Last update:

No citation recorded.