DOI: https://doi.org/10.14710/jbtr.v9i2.17290

The Activity of Liposome-Parijoto Formula Through p53 Expression in HepG2 Cell Line

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 27 Jan 2023; Revised: 26 Apr 2023; Accepted: 5 Jun 2023; Available online: 31 Aug 2023; Published: 31 Aug 2023.
Open Access Copyright (c) 2023 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Parijoto, one of Melastomaceae family, has been known to have cytotoxic activity in HepG2, a hepatocellular cancer cell line, but with low activity. However, the ethyl acetate fraction of Parijoto gave the highest antioxidant and cytotoxic activity in 4T1. Then, purification and liposome formulation need to be carried out to increase the activity of Parijoto extract.  Objective: This research aimed to study the cytotoxic activity and molecular mechanism of LEA (Liposom-Ethyl Acetate of Parijoto Fraction) in HepG2. Method: Extraction has been done by maceration, followed by partition using n-hexane, ethyl acetate, and methanol. LEA formulation was carried out by thin-layer hydration with modification and the formula was sized using a bath sonicator.  Cytotoxic activity test of LEA and extract was performed in five serial concentrations (3,9 µg/mL–250 µg/mL), while the positive control doxorubicin performed in 3,9 µg/mL – 250 µg/mL by MTT assay. P53 gene expression was analyzed by using PCR-electrophoresis. Result: Results showed that LEA increased the cytotoxic activity (IC50 = 28.40 μg/ml). Furthermore, based on the electrophoresis study, LEA induced the p53 expression while the extract only did not. Conclusion: Liposome formula from ethyl acetate fraction of Parijoto extract (LEA) was able to increase cytotoxic activity and p53 gene expression was possible through the apoptotic mechanism. This shows that this formula is a promising strategy to improve the bioavailability of herbal medicines as cytotoxic agents.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Materials
PCR-Primers Used
Subject primer
Type Research Materials
  Download (10KB)    Indexing metadata
Keywords: cytotoxic; formulation; bioavailability; Parijoto extract

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
Increased Level of The Plasminogen Activator Inhibitor Type-1 is Associated with Severity of NAFLD Effect Of Zinc Administration in Preventing Ethambutol-Induced Optic Neuropathy in Wistar Rats Model The Activity of Liposome-Parijoto Formula Through p53 Expression in HepG2 Cell Line Plasma Exchange as A Rescue Therapy in Weil’s Disease with Severe Hyperbilirubinemia, Acute Renal Failure, And Multidrug-Resistant Organism Co-Infection: A Rare Case in Critical Care Setting Mechanism of Immune System Dysfunction, Apoptosis and Oxidative Stress on Endometriosis More recent articles
  1. M.L Martínez-Chantar, M.A. Avila, S.C. Lu, Hepatocellular Carcinoma: Updates in Pathogenesis, Detection and Treatment, Cancers 2020, 12, 2729; doi: 10.3390/cancers12102729
  2. Y.Li, and M.I. Robert C.G, Review Article: Herbal Medicine and Hepatocellular Carcinoma: Applications and Challenges. Evidence-Based Complementary and Alternative Medicine, Volume 2011, Article ID 541209, 14 pages, doi: 10.1093/ecam/neq044
  3. w. sasikirana, e. Annisaa', n. ekawati, I. R. Eka Dini, and E. Tumbilaka, "Selectivity of Ethanol Extract of Parijoto (Medinilla speciosa) Fruit in HepG2, Widr, 4T1, and Vero cell lines," Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal), vol. 10, no. 5, pp. 337-341, Sep. 2021. https://doi.org/10.14710/dmj.v10i5.32010
  4. E.Annisaa;, W.Sasikirana, N.Ekawati, and I.R. Eka Dini, Correlation Between Antioxidant and Cytotoxic Activity of Parijoto (Medinilla speciosa Blume) Fractions in 4T1 Cell Line. Indones. J. Cancer Chemoprevent., 12(1). 21-27
  5. Mi-Kyung Lee. Review: Liposome for Enhanced Bioavaibility of Water-Insoluble Drugs: In Vivo Evidence and Recent Approaches. Pharmaceutics 2020, 12, 264; doi: 10.3390/pharmaceutics12030264
  6. Takashi Yoshida, Yoshitaka Ikeda, Hideyaki Ohbayashi, Koukichi Ishihara, Wakayo Ohwashi, Tetsuro Shingu, and Takuo Okuda, Dimeric Ellagitannins in Plants of Melastomaceae, Communication to the Editor, Chem. Pharm.Bull, 34(6) 2676-2679 (1986)
  7. Takashi Yoshida *, Yoshiaki Amakura and Morio Yoshimura. Review: Structural Features and Biological Properties of Ellagitannins in Some Plant Families of the Order Myrtales. Int. J. Mol. Sci. 2010, 11, 79-106; doi: 10.3390/ijms11010079
  8. Wang Z, Jiang Y, Guan D, Li J, Yin H, et al. (2013) Critical Roles of p53 in Epithelial-Mesenchymal Transition and Metastasis of Hepatocellular Carcinoma Cells. PLOS ONE 8(9): e72846. https://doi.org/10.1371/journal.pone.0072846
  9. Tim Link and Tomoo Iwakuma. Roles of p53 in extrinsic factor-induced liver carcinogenesis. Hepatoma Res. 2017; 3: 95–104. doi: 10.20517/2394-5079.2017.07
  10. Mabrouk Zayed, M.M.; Sahyon, H.A.; Hanafy, N.A.N.; El-Kemary, M.A. The Effect of Encapsulated Apigenin Nanoparticles on HePG-2 Cells through Regulation of P53. Pharmaceutics 2022, 14, 1160
  11. Niswah, Lukluatun. Uji Aktivitas Antibakteri dari Ekstrak Buah Parijoto (Medinilla speciosa Blume) menggunakan Metode Difusi Cakram. Skripsi.2014. Program Studi Farmasi. Fakultas Kedokteran dan Ilmu Kesehatan. UIN Syarif Hidayatullah Jakarta
  12. Granado-Serrano, A.B.; Martín, M.A.; Bravo, L.; Goya, L.; Ramos, S. Quercetin induces apoptosis via caspase activation,regulation of Bcl-2, and inhibition of PI-3-kinase/Akt and ERK pathways in a human hepatoma cell line (HepG2). J. Nutr. 2006,136, 2715–2721
  13. Li, N.; Liu, J.-H.; Zhang, J.; Yu, B.-Y. Comparative evaluation of cytotoxicity and antioxidative activity of 20 flavonoids. J. Agric.Food Chem. 2008, 56, 3876–3883
  14. Izabela Berdowska, Małgorzata Matusiewicz, and Izabela Fecka, Review: Punicalagin in Cancer Prevention—Via Signaling Pathways Targeting, Nutrients 2021, 13, 2733. https://doi.org/10.3390/nu13082733
  15. Tariq Ismail , Cinzia Calcabrini , Anna Rita Diaz , Carmela Fimognari , Eleonora Turrini, Elena Catanzaro , Saeed Akhtar, and Piero Sestili, Review: Ellagitannins in Cancer Chemoprevention and Therapy, Toxins 2016, 8, 151; doi: 10.3390/toxins8050151

Last update:

No citation recorded.

Last update:

No citation recorded.