DOI: https://doi.org/10.14710/jbtr.v9i1.16824

Effect of Probiotic Supplementation on FSH, LH Levels and Folliculogenesis

Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 22 Dec 2023; Revised: 11 Apr 2023; Accepted: 27 Apr 2023; Available online: 30 Apr 2023; Published: 29 Apr 2023.
Open Access Copyright (c) 2023 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder that often occurs in women of reproductive age. The main therapy currently used to treat PCOS patients is insulin sensitizer. In PCOS, there is an imbalance in the intestinal flora which causes activation of the immune system and an inflammatory response that leads to insulin resistance. The effect of probiotic supplementation on insulin resistance can have an impact on changes in reproductive hormone levels in PCOS women. 

Objective: Analyze the effect of probiotic supplementation on levels of FSH, LH, and folliculogenesis in a study of PCOS Wistar rats. 

Methods: Experimental research with a post-test only controlled group design. The research sample was 35 Wistar rats which were divided into 5 groups, (K-) were healthy rats, (K+) were PCOS rats without treatment, (P1) were PCOS rats received Metformin, (P2) were PCOS rats received Probiotics, and (P3) were PCOS rats that received Metformin + Probiotics. The intervention was carried out for 14 days. The dependent variables were levels of FSH, LH and folliculogenesis. Research data were analyzed using one-way ANOVA test, Fisher Exact test. Differences between groups and controls were tested with Dunnett's post hoc test. Significant p<0.05

Results: Metformin + probiotic supplementation resulted in increased FSH levels, decreased LH levels and increased folliculogenesis when compared to the PCOS rats without treatment (K+).

Conclusion: Metformin + probiotic supplementation causes levels of FSH, LH and folliculogenesis activity in PCOS rats to resemble healthy rats.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Results
Derajat Folikulogenesis
Subject
Type Research Results
  Download (696KB)    Indexing metadata
Keywords: PCOS; Metformin; Probiotic; LH; FSH; Folliculogenesis

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
Effect of Probiotic Supplementation on FSH, LH Levels and Folliculogenesis The Effect of Liprotide-Encapsulated Vitamin D3 on MDA and SOD in Rats Deficient Vitamin D and Calcium Potential of B-Cell Epitopes Protein Ag85 Complex Mycobacterium Tuberculosis as Serodiagnostic Antigen of Tuberculosis by in Silico Study Understanding Platelet-Rich Plasma as Potential Therapy To Improve Cardiac Function After Myocardial Infarction: Based On Evidence More recent articles
  1. Osuka S, Nakanishi N, Murase T, Nakamura T, Goto M, Iwase A, et al. Animal models of polycystic ovary syndrome: A review of hormone‐induced rodent models focused on hypothalamus‐pituitary‐ovary axis and neuropeptides. Reprod Med Biol. 2019 Apr 28;18(2):151–60. DOI: 10.1002/rmb2.12262
  2. Brassard M, AinMelk Y, Baillargeon JP. Basic Infertility Including Polycystic Ovary Syndrome. Medical Clinics of North America. 2008 Sep;92(5):1163–92. DOI: 10.1016/j.mcna.2008.04.008
  3. Fauser BCJM, Tarlatzis BC, Rebar RW, Legro RS, Balen AH, Lobo R, et al. Consensus on women’s health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil Steril. 2012 Jan;97(1):28-38.e25. DOI: 10.1016/j.fertnstert.2011.09.024
  4. Heshmati J, Farsi F, Yosaee S, Razavi M, Rezaeinejad M, Karimie E, et al. The Effects of Probiotics or Synbiotics Supplementation in Women with Polycystic Ovarian Syndrome: a Systematic Review and Meta-Analysis of Randomized Clinical Trials. Probiotics Antimicrob Proteins. 2019 Dec 13;11(4):1236–47. DOI: 10.1007/s12602-018-9493-9
  5. Tremellen K, Pearce K. Dysbiosis of Gut Microbiota (DOGMA) – A novel theory for the development of Polycystic Ovarian Syndrome. Med Hypotheses. 2012 Jul;79(1):104–12. DOI: 10.1016/j.mehy.2012.04.016
  6. Khashchenko E, Uvarova E, Vysokikh M, Ivanets T, Krechetova L, Tarasova N, et al. The Relevant Hormonal Levels and Diagnostic Features of Polycystic Ovary Syndrome in Adolescents. J Clin Med. 2020 Jun 11;9(6):1831. DOI: 10.3390/jcm9061831
  7. Jindal P, Mehandiratta R, Sharma A, Takkar V, Kapila PT. Effect of Metformin on FSH, LH and Prolactin Levels in Patients with Polycystic Ovarian Syndrome. International Journal of Medical Research Professionals. 2016 Sep;2(5). DOI: 10.21276/ijmrp.2016.2.5.037
  8. Dardmeh F, Alipour H, Gazerani P, van der Horst G, Brandsborg E, Nielsen HI. Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model. PLoS One. 2017 Oct 10;12(10):e0185964. DOI: 10.1371/journal.pone.0185964
  9. Karimi E, Heshmati J, Shirzad N, Vesali S, Hosseinzadeh-Attar MJ, Moini A, et al. The effect of synbiotics supplementation on anthropometric indicators and lipid profiles in women with polycystic ovary syndrome: a randomized controlled trial. Lipids Health Dis. 2020 Dec 6;19(1):60. DOI: 10.1186/s12944-020-01244-4
  10. Rice S, Elia A, Jawad Z, Pellatt L, Mason HD. Metformin Inhibits Follicle-Stimulating Hormone (FSH) Action in Human Granulosa Cells: Relevance to Polycystic Ovary Syndrome. J Clin Endocrinol Metab. 2013 Sep 1;98(9):E1491–500. DOI: 10.1210/jc.2013-1865
  11. Thessaloniki ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Consensus on infertility treatment related to polycystic ovary syndrome. Human Reproduction. 2008 Mar 1;23(3):462–77. DOI: 10.1093/humrep/dem426
  12. Dabke K, Hendrick G, Devkota S. The gut microbiome and metabolic syndrome. Journal of Clinical Investigation. 2019 Oct 1;129(10):4050–7. DOI: 10.1172/JCI129194
  13. Ratajczak W, Rył A, Mizerski A, Walczakiewicz K, Sipak O, Laszczyńska M. Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs). Acta Biochim Pol. 2019 Mar 4; DOI: 10.18388/abp.2018_2648
  14. Zhang J, Sun Z, Jiang S, Bai X, Ma C, Peng Q, et al. Probiotic Bifidobacterium lactis V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis. mSystems. 2019 Apr 30;4(2). DOI: 10.1128/mSystems.00017-19
  15. Guo Y, Qi Y, Yang X, Zhao L, Wen S, Liu Y, et al. Association between Polycystic Ovary Syndrome and Gut Microbiota. PLoS One. 2016 Apr 19;11(4):e0153196. DOI: 10.1371/journal.pone.0153196
  16. Arab A, Hossein-Boroujerdi M, Moini A, Sepidarkish M, Shirzad N, Karimi E. Effects of probiotic supplementation on hormonal and clinical outcomes of women diagnosed with polycystic ovary syndrome: A double-blind, randomized, placebo-controlled clinical trial. J Funct Foods. 2022 Sep;96:105203. DOI: 10.1016/j.jff.2022.105203
  17. Shamasbi SG, Ghanbari-Homayi S, Mirghafourvand M. The effect of probiotics, prebiotics, and synbiotics on hormonal and inflammatory indices in women with polycystic ovary syndrome: a systematic review and meta-analysis. Eur J Nutr. 2020 Mar 29;59(2):433–50. DOI: 10.1007/s00394-019-02033-1
  18. Hadi A, Ghaedi E, Khalesi S, Pourmasoumi M, Arab A. Effects of synbiotic consumption on lipid profile: a systematic review and meta-analysis of randomized controlled clinical trials. Eur J Nutr. 2020 Oct 22;59(7):2857–74. DOI: 10.1007/s00394-020-02248-7
  19. Szydłowska I, Marciniak A, Brodowska A, Loj B, Ciećwież S, Skonieczna-Żydecka K, et al. Effects of probiotics supplementation on the hormone and body mass index in perimenopausal and postmenopausal women using the standardized diet. A 5-week double-blind, placebo-controlled, and randomized clinical study. Eur Rev Med Pharmacol Sci. 2021;25(10):3859–67. DOI: 10.26355/eurrev_202105_25953
  20. Diamanti-Kandarakis E, Dunaif A. Insulin Resistance and the Polycystic Ovary Syndrome Revisited: An Update on Mechanisms and Implications. Endocr Rev. 2012 Dec 1;33(6):981–1030. DOI: 10.1210/er.2011-1034
  21. Dumesic DA, Padmanabhan V, Abbott DH. Polycystic Ovary Syndrome and Oocyte Developmental Competence. Obstet Gynecol Surv. 2008 Jan;63(1):39–48. DOI: 10.1097/OGX.0b013e31815e85fc

Last update:

No citation recorded.

Last update:

No citation recorded.