1Faculty of Medicine, Universitas Diponegoro, Indonesia
2Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Indonesia
3Department of Pathology Anatomy, Faculty of Medicine, Universitas Diponegoro, Indonesia
4 Department of Anatomy-Histology, Faculty of Medicine, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JBTR17286, author = {Adhika Bastian Prananta and Nyoman Widyastiti and Ariosta Ariosta and Dwi Retnoningrum and Rezya Salsabela and Vega Karlowee and Neni Susilaningsih}, title = {Renoprotective Effect Of Sambiloto (Andrographis Paniculata) Leaf Extract On Lipopolysaccharide – Induced Septic Rats}, journal = {Journal of Biomedicine and Translational Research}, volume = {9}, number = {1}, year = {2023}, keywords = {Andrographis paniculata; creatinine; uric acid; lipopolysaccharide; AKI; sepsis}, abstract = { Background: Associated with sepsis is the illness known as acute kidney injury (AKI). AKI may be mediated by uric acid, and blood creatinine levels can be utilized to diagnose the condition to measure kidney function. Sambiloto (Andrographis paniculata) is a traditional medicine that has flavonoid compounds that can reduce creatinine levels and Xanthine Oxidase Inhibitors which can reduce uric acid levels. Objective: Septic model rats generated by lipopolysaccharide were used to test the effects of sambiloto (Andrographis paniculata) leaf extract on serum creatinine and uric acid levels (LPS). Methods: This study was experimental employing 25 rats split into 5 groups as the post-test alone control group: healthy control with standard feed (HC), negative control with LPS injection(NC), Treatment (T)1 (A.Paniculata 200 mg/kgBW+LPS), T2(A.paniculata 400 mg/kgBW+LPS), and T3(A.paniculata 500mg/kgBW+ LPS). A.paniculata leaf extract was given via oral gavage on day 8-21. Intraperitoneal injection of LPS 5 mg/kgBW was given on day 22. On the 25 th day, the blood serum was analyzed for creatinine levels using Jaffe method and uric acid was analyzed using the enzymatic photometric method. One-way analysis of variance (Kruskal-Wallis) and the Kruskal-Wallis test were used to evaluate the data. Results: The mean creatinine levels of HC,NC,T1,T2,T3 were 0.7±0.01;3.5±0.04;2.9±0.03;1.9±0.05; 1.3 ±0.04 mg/dl respectively. The mean uric acid levels of HC.NC.T1.T2.T3 were 1.7±0.05;8.2±0.11; 4.5±0.03;4.0±0.12;3.0±0.19 mg/dl respectively. There were significant differences (p<0.05) in creatinine levels in groups T2 (p=0.031) and T3 (p=0.001) to NC group and serum uric acid levels in groups T1 (p<0.001) , T2 (p<0.001) , and T3 (p<0.001 ) to NC group which creatinine and uric acid levels were lower than NC group. Conclusion: Andrographis paniculata leaf extract has renoprotective effect against AKI in LPS-induced septic rats Keywords: Andrographis paniculata, creatinine, uric acid, lipopolysaccharide, AKI, sepsis }, issn = {2503-2178}, pages = {25--30} doi = {10.14710/jbtr.v9i1.17286}, url = {https://ejournal2.undip.ac.id/index.php/jbtr/article/view/17286} }
Refworks Citation Data :
Background: Associated with sepsis is the illness known as acute kidney injury (AKI). AKI may be mediated by uric acid, and blood creatinine levels can be utilized to diagnose the condition to measure kidney function. Sambiloto (Andrographis paniculata) is a traditional medicine that has flavonoid compounds that can reduce creatinine levels and Xanthine Oxidase Inhibitors which can reduce uric acid levels.
Objective: Septic model rats generated by lipopolysaccharide were used to test the effects of sambiloto (Andrographis paniculata) leaf extract on serum creatinine and uric acid levels (LPS).
Methods: This study was experimental employing 25 rats split into 5 groups as the post-test alone control group: healthy control with standard feed (HC), negative control with LPS injection(NC), Treatment (T)1 (A.Paniculata 200 mg/kgBW+LPS), T2(A.paniculata 400 mg/kgBW+LPS), and T3(A.paniculata 500mg/kgBW+ LPS). A.paniculata leaf extract was given via oral gavage on day 8-21. Intraperitoneal injection of LPS 5 mg/kgBW was given on day 22. On the 25th day, the blood serum was analyzed for creatinine levels using Jaffe method and uric acid was analyzed using the enzymatic photometric method. One-way analysis of variance (Kruskal-Wallis) and the Kruskal-Wallis test were used to evaluate the data.
Results: The mean creatinine levels of HC,NC,T1,T2,T3 were 0.7±0.01;3.5±0.04;2.9±0.03;1.9±0.05; 1.3 ±0.04 mg/dl respectively. The mean uric acid levels of HC.NC.T1.T2.T3 were 1.7±0.05;8.2±0.11; 4.5±0.03;4.0±0.12;3.0±0.19 mg/dl respectively. There were significant differences (p<0.05) in creatinine levels in groups T2(p=0.031) and T3(p=0.001) to NC group and serum uric acid levels in groups T1(p<0.001), T2(p<0.001), and T3(p<0.001) to NC group which creatinine and uric acid levels were lower than NC group.
Conclusion: Andrographis paniculata leaf extract has renoprotective effect against AKI in LPS-induced septic rats
Keywords: Andrographis paniculata, creatinine, uric acid, lipopolysaccharide, AKI, sepsis
Article Metrics:
Last update:
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Journal of Biomedicine and Translational Research Diponegoro University as publisher of the journal.
Copyright encompasses non-exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations.
Journal of Biomedicine and Translational Research Diponegoro University, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the Journal of Biomedicine and Translational Research Diponegoro University (JBTR) are sole and exclusive responsibility of their respective authors and advertisers.
The Copyright Transfer Form can be downloaded here: [Copyright Transfer Form JBTR]
The copyright form should be signed originally and send to the Editorial Office in the form of original mail, scanned document or fax : Journal of Biomedicine and Translational Research Faculty of Medicine, Diponegoro UniversityJl. Prof. Soedarto, Kampus UNDIP Tembalang, Semarang, Central Java, Indonesia 50275, Telp.: +62-24-8454714, Fax.: +62-24-8454714Email: jbtr@fk.undip.ac.id
JBTR by https://ejournal2.undip.ac.id/index.php/jbtr is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats