DOI: https://doi.org/10.14710/jbtr.v9i1.16470

Detection of Blastocystis Hominis by Method of Cultivation in The Feces of Orphanage Children in Pekanbaru, Riau Province, Indonesia

1Department of Parasitology, Faculty of Medicine, Universitas Riau, Indonesia

2Faculty of Medicine, Universitas Riau, Indonesia

3Department of Parasitology, Faculty of Medicine, Universitas Kristen Indonesia, Indonesia

Received: 16 Nov 2022; Revised: 16 Mar 2023; Accepted: 28 Apr 2023; Available online: 30 Apr 2023; Published: 29 Apr 2023.
Open Access Copyright (c) 2023 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Blastocystis hominis is an intestinal protozoan that can infect humans and animals. The distribution coverage is very wide and is transmitted through the fecal-oral route. The incidence of blastocystosis due to Blastocystis hominis is higher in developing countries because it is associated with poor hygiene practice, inadequate sanitation, close contact with pets domesticated animals and or contaminated food. Blastocystis hominis infection can cause clinical manifestations, from asymptomatic to chronic diarrhea, depending on the Blastocystis subtype and the patient's immune system. brief background of the topic and significance of the study.

Objective: The aim of this study was to detect and determine the incidence of Blastocystis hominis infection in the feces of children at the Pekanbaru Orphanage using the culture method.

Methods: The sample is the feces of children from nine orphanages in Pekanbaru. Detection of Blastocystis by the modified Jone's Medium culture method but using sheep's serum

Results: A total of 95 children's stool samples were examined from 9 orphanages in Pekanbaru, it was found that 63 children (66.3%) were positive for Blastocystis hominis using the culture method, positive Blastocystis hominis was found more in boys (58.7%%) than girls, and based on age group, 6-12 years were found to be the age group with the most infection (84.1%). Generally, the source of drinking water in orphanages is refilled drinking water and all orphanages have cats as pets, and a few have chickens, birds, and goats as pets

Conclusion: High incidence of Blastocystis hominis can be detected in the feces of Pekanbaru orphanage children using the culture method.

 

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Keywords: Blastocystis; children; culture; orphanage;

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Language : EN
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  9. Sari IP, Benung MR, Wahdini S, Kurniawan A. Diagnosis and identification of Blastocystis subtypes in primary school children in Jakarta. J Trop Pediatr. 2018;64(3):208–14
  10. Bodnia I, Pokhil S, Bodnia K, Pavliy V, Skoryk L. Distribution and frequency of Blastocystis sp. by methods of microscopy and cultivation in faeces of residents of Kahrkov Region. Georgian Med News. 2022;7(328):85–90
  11. Giezen M Van der. Modified Jones’ Medium for in-vitro culture of Blastocystis. Internet [Internet]. 2016; Available from: http://www.blastocystis.net/p/lab-stuff.html
  12. Jalallou N, Iravani S, Rezaeian M, Alinaghizade A, Mirjalali H. Subtypes distribution and frequency of Blastocystis sp. Isolated from diarrheic and non-diarrheic patients. Iran J Parasitol. 2017;12(1):63–8
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  14. Cinek O, Polackova K, Odeh R, Alassaf A, Kramná L, Ibekwe MAU, et al. Blastocystis in the faeces of children from six distant countries: prevalence, quantity, subtypes and the relation to the gut bacteriome. Parasites and Vectors. 2021;14(1):1–17
  15. Shams M, Shamsi L, Yousefi A, Sadrebazzaz A, Asghari A, Mohammadi-Ghalehbin B, et al. Current global status, subtype distribution and zoonotic significance of Blastocystis in dogs and cats: a systematic review and meta-analysis. Parasites and Vectors. 2022;15(1):1–17
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  17. Al-Shamiri AH, Alzubairy AH, Al-Mamari RF. The prevalence of cryptosporidium spp. in children, Taiz District, Yemen. Iran J Parasitol. 2010;5(2):26–32
  18. Chang T, Jung BK, Shin H, Hong S, Ryoo S, Lee J, et al. Genotypes of Blastocystis sp. among elderly health checkup people in South Korea with a questionnaire on risk factors. Parasitol Res. 2021;120(9):3297–306
  19. Onder Z, Yildirim A, Pekmezci D, Duzlu O, Pekmezci GZ, Ciloglu A, et al. Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey. Acta Trop [Internet]. 2021;220(March):105939. Available from: https://doi.org/10.1016/j.actatropica.2021.105939
  20. Udonsom R, Prasertbun R, Mahittikorn A, Mori H, Changbunjong T, Komalamisra C, et al. Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand. Infect Genet Evol [Internet]. 2018;65:107–11. Available from: https://doi.org/10.1016/j.meegid.2018.07.007

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