DOI: https://doi.org/10.14710/jbtr.v8i3.15442

The Effect of Roselle Flower Infusion (Hibiscus sabdariffa) on Retinal Ganglion Cell Apoptosis of Sprague Dawley Rats Exposed to Cigarette Smoke

1Ophthalmology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Biology and Biochemistry Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

3Histopathology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

4 Anatomy Pathology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

5 Faculty of Medicine, Universitas Diponegoro, Indonesia

6 Biology and Biochemistry Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

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Received: 10 Aug 2022; Revised: 3 Nov 2022; Accepted: 3 Nov 2022; Available online: 30 Dec 2022; Published: 30 Dec 2022.
Open Access Copyright (c) 2022 Journal of Biomedicine and Translational Research
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Abstract

Background: Cigarette smoke contains a lot of free radicals that can reduce antioxidants in the body. One of those is Reactive Oxygen Species (ROS) which can induce Retinal Ganglion Cell (RGC) apoptosis. Reduced ganglion cell axons will eventually lead to optic neuropathy. Supplementing herbal medicines, vitamins, foods, and drinks containing antioxidants is one way to increase oxidant levels in the body. Roselle or Hibiscus sabdariffa is a herbal plant reported to have a lot of antioxidant content.

Objective: The study aimed to prove that Roselle flower infusions can reduce retinal ganglion cell apoptosis of Sprague Dawley rats exposed to cigarette smoke.

Methods: This was an experimental study with a post-test-only design with a total of 24 male Sprague Dawley rats exposed to four non-filtered cigarettes/day for 30 consecutive days. They were randomly divided into four sub-groups, and each group consisted of 6 rats. The control group received 1 ml of saline, and three treatment groups received Roselle flower infusions of 2.5%, 5%, and 10% for 30 days. Enucleated eyes were then made paraffin blocks and slides with HE staining. The retinal ganglion cell apoptosis score was assessed by calculating the number of apoptotic cells using 1000x in five fields of view. The data were analyzed using Kruskal-Wallis non-parametric test followed by the Mann-Whitney U test.

Results: The average retinal ganglion cell apoptosis score in control, treatment 1, 2, and 3 groups were 3.00; 2.83; 2.00; 1.83. There was a significant difference between the control group and treatment groups 2 (p=0.001) and group 3 (p=0.001), while between the control group and treatment group 1, there was no significant difference (p=0.317).

Conclusion: The administration of 5% and 10% Roselle flower infusions can reduce the apoptosis score of retinal ganglion cells in Sprague Dawley rats exposed to cigarette smoke. The higher the Roselle flower infusion concentration, the lower the apoptosis score of retinal ganglion cells.

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Keywords: Hibiscus sabdariffa; Retinal Ganglion Cell; Apoptosis; Cigarette Smoke

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Section: Original Research Articles
Language : EN
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  1. Ministry of Health of Republic Indonesia. Major Results of Basic Health Research 2018. 2018. Available from: https://kesmas.kemkes.go.id/assets/upload/dir_519d41d8cd98f00/files/Hasil-riskesdas-2018_1274.pdf
  2. Ministry of Health of Republic Indonesia. The government strives to reduce the number of beginner smokers. (15 May 2020). Citing Internet Sources: https://www.kemkes.go.id/article/view/17060200002/pemerintah-upayakan-pengurangan-jumlah-perokok-pemula-.html
  3. Fitria, Triandita RINKR, Mangimbulude JC, Karwur FF. Smoking and DNA Oxidation. Sains Med. 2013;5:113–20. Available from: http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/352/291
  4. U.S. Department of Health and Human Services. How Tobacco Smoke Causes Disease: The Biology and Behavioral Basis for Smoking-Attributable Disease. 2010. 792 p. Available from: PMID 21452462
  5. Grzybowski A, Holder GE. Tobacco optic neuropathy (TON) - The historical and present concept of the disease. Acta Ophthalmol. 2011;89(5):495–9. doi: 10.1111/j.1755-3768.2009.01853.x
  6. Bhatti MT, Biousse V, Bose S, Danesh-Mayer H V., Levin LA, Phillips PH, et al., editors. 2020-2021 Basic and Clinical Science Course : 5. Neuro-Ophthalmology. San Fransisco: American Academy of Ophthalmology; 2017
  7. Phillips KC, Clarke-Farr PC. Ethambutol toxic optic neuropathy: A case report and review. African Vis Eye Heal. 2018;77(1):1–3. doi: https://doi.org/10.4102/aveh.v77i1.419
  8. Nowomiejska K, Kiszka A, Maciejewski R, Jünemann A, Rejdak R. Central scotoma in tobacco-alcohol toxic optic neuropathy measured with semi-automated kinetic perimetry. Cutan Ocul Toxicol. 2018;37(4):319–23. doi: https://doi.org/10.1080/15569527.2018.1459666
  9. Chiotoroiu SM, Noaghi M, Stefaniu GI, Secureanu FA, Purcarea VL, Zemba M. Tobacco-alcohol optic neuropathy--clinical challenges in diagnosis. J Med Life [Internet]. 2014;7(4):472–6. Available from: PMCID: PMC4316121
  10. Arda H, Mirza GE, Polat OA, Karakucuk S, Oner A, Gumus K. Effects of chronic smoking on color vision in young subjects. Int J Ophthalmol. 2015;8(1):77–80. doi: https://doi.org/10.3980/j.issn.2222-3959.2015.01.14
  11. Grzybowski A, Zülsdorff M, Wilhelm H, Tonagel F. Toxic optic neuropathies: An updated review. Acta Ophthalmol. 2015;93(5):402–10. doi: 10.1111/aos.12515
  12. Fernandes TM de P, Almeida NL, dos Santos NA. Comparison of color discrimination in chronic heavy smokers and healthy subjects. F1000Research. 2017;6(0):85. doi: 10.12688/f1000research.10714.1
  13. Sharma P, Sharma R. Toxic optic neuropathy. Indian J Ophthalmol. 2011;59(2):137–41. doi: 10.4103/0301-4738.77035
  14. Valavanidis A, Vlachogianni T, Fiotakis K. Tobacco smoke: Involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles. Int J Environ Res Public Health. 2009;6(2):445–62. doi: 10.3390/ijerph6020445
  15. Caliri AW, Tommasi S, Besaratinia A. Relationship among smoking, oxidative stress, inflammation, macromolecular damage, and cancer. Mutat Res. 2021;787:108365. doi: 10.1016/j.mrrev.2021.108365
  16. Rusmana D, Wahyudianingsih R, Elisabeth M, Balqis, Maesaroh, Widowati W. Antioxidant activity of Phyllanthus niruri extract, rutin and quercetin. Indones Biomed J. 2017;9(2):84–90. doi: 10.18585/inabj.v9i2.281
  17. Widhiantara IG, Permatasari AAAP, Rosiana IW, Wiradana PA, Widiastini LP, Jawi IM. Antihypercholesterolemic and Antioxidant Effects of Blumea balsamifera L. Leaf Extracts to Maintain Luteinizing Hormone Secretion in Rats Induced by High-Cholesterol Diets. Indones Biomed J. 2021;13(4):396–402. doi: 10.18585/INABJ.V13I4.1694
  18. Nurhayati B, Rahayu IG, Rinaldi SF, Zaini WS, Afifah E, Arumwardana S, et al. The antioxidant and cytotoxic effects of Cosmos caudatus Ethanolic Extract on Cervical Cancer. Indones Biomed J. 2018;10(3):243–9. doi: 10.18585/inabj.v10i3.441
  19. Kusnadi, Purgiyanti. Quantification of polyphenol, antioxidant, and antibacterial from red and purple roselle calyces using maceration extraction under different solvent conditions. J Phys Conf Ser. 2021;1918(5). doi: 10.1088/1742-6596/1918/5/052007
  20. Suseno R, Surhaini, Rahmi SL, Yanti F. Characteristics and sensory properties of lemongrass, Roselle, and ginger formulation herbal tea. IOP Conf Ser Earth Environ Sci. 2022;951(1). doi: 10.1088/1755-1315/951/1/012096
  21. Tsai PJ, McIntosh J, Pearce P, Camden B, Jordan BR. Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.) extract. Food Res Int. 2002;35(4):351–6. doi: 10.1016/S0963-9969(01)00129-6
  22. Fat a A-MG, Farouk SM, Emam MA. Antiapoptotic and antioxidant capacity of phytochemicals from Roselle (Hibiscus sabdariffa)and their potential effects on monosodium glutamate-induced testicular damage in rat. Environ Sci Pollut Res. 2021;28(47):67901–3. doi: https://doi.org/10.1007/s11356-020-10674-7
  23. Bakhtiari E, Hosseini A, Mousavi SH. Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury. Avicenna J phytomedicine. 2015;5(3):231–7. PMID: 26101756
  24. Syahidah A, Intan A, Tari N, Widyastuti R. Chemical and Organoleptics of Red Rosella Tea Powder (Hibiscus Sabdariffa Linn) with Drying Time Variatios. 2022;2(1):46–56. doi: https://doi.org/10.32585/jfap.v2i1.2336
  25. Lee K, Hong S, Seong GJ, Kim CY. Cigarette Smoke Extract Causes Injury in Primary Retinal Ganglion Cells via Apoptosis and Autophagy. Curr Eye Res. 2016;41(10):1367–72. doi: 10.3109/02713683.2015.1119856
  26. Moschos MM, Nitoda E, Laios K, Ladas DS, Chatziralli IP. The Impact of Chronic Tobacco Smoking on Retinal and Choroidal Thickness in Greek Population. Oxid Med Cell Longev. 2016;2016. doi: 10.1155/2016/2905789
  27. Yang TK, Huang XG, Yao JY. Effects of Cigarette Smoking on Retinal and Choroidal Thickness: A Systematic Review and Meta-Analysis. J Ophthalmol. 2019;2019:8079127. doi: 10.1155/2019/8079127
  28. Karademirci M, Kutlu R, Kilinc I. Relationship between smoking and total antioxidant status, total oxidant status, oxidative stress index, vit C, vit E. Clin Respir J. 2018;12(6):2006–12. doi: 10.1111/crj.12757

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