DOI: https://doi.org/10.14710/jbtr.v8i2.15323

Correlation between Interleukin-17 Levels with C-Reactive Protein and Neutrophil Lymphocyte Ratio in Sepsis

1Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Department of Internal Medicine, Faculty of Medicine, Universitas Diponegoro, Indonesia

3Department of Anesthesiology and Intensive Care, Faculty of Medicine, Universitas Diponegoro, Indonesia

4 Department of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia

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Received: 26 Jul 2022; Revised: 23 Aug 2022; Accepted: 25 Aug 2022; Available online: 31 Aug 2022; Published: 31 Aug 2022.
Open Access Copyright (c) 2022 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Sepsis is a condition in systemic infection associated with organ
dysfunction. Interleukin-17 is a pro-inflammatory cytokine produced by Th-17 cells. C-
reactive protein and neutrophil-lymphocyte ratio (NLR) have been widely used as
markers of inflammation. The relationship between IL-17 as a proinflammatory
cytokine with CRP and NLR has not been reported.
Objective: This study aims to prove the correlation between IL-17 with CRP and NLR
in septic patients.
Methods: Analytical observational study with a cross-sectional approach was carried
out on 40 septic patients in July–December 2020. IL-17 levels were obtained by the
ELISA method while CRP levels were obtained using the immunoturbidimetric method.
NLR was the result of dividing the absolute number of neutrophils and lymphocytes
from the automatic hematology analyzer. Correlation between variables was performed
using Spearman correlation test.
Results: The median levels of IL-17, CPR, and NLR were 363.55 (11.4-1695.80)
pg/mL, 13.25 (0.43-53.87) mg/L, and 12.00 (2.26–48.5), respectively. The Spearman
correlation test between IL-17 levels and CRP obtained p = 0.019, r = 0.37, and NLR p
= 0.425, r = 0.13.
Conclusion: There is a weak positive correlation between IL-17 levels and CRP in
septic patients. There is no correlation between IL-17 levels and NLR in septic patients.

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Keywords: Interleukin-17, CRP, NLR, sepsis

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  2. Mayr FB, Yende S, Angus DC. Epidemiology of severe sepsis. Virulence [Internet]. 2014;5(1):1–11. Available from: https://doi.org /10.4161/viru.27372
  3. Álvaro-Meca A, Jiménez-Sousa MA, Micheloud D, Sánchez-Lopez A, Heredia- Rodríguez M, Tamayo E, et al. Epidemiological trends of sepsis in the twenty-first century (2000-2013): An analysis of incidence, mortality, and associated costs in Spain. Popul Health Metr [Internet]. 2018;16(1):1–11. Available from:
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  5. Burkovskiy I, Sardinha J, Zhou J, Lehmann C. Cytokine release in sepsis. Adv Biosci Biotechnol [Internet]. 2013;2013(September):860–5. Available from: http://dx.doi.org/10.4236/abb.2013.49114
  6. Flierl MA, Rittirsch D, Gao H, Hoesel LM, Nadeau BA, Day DE, et al. Adverse functions of IL-17A in experimental sepsis. FASEB J [Internet]. 2008;22(July):2199–205. Available from: https://doi.org/10.1096/fj.07-105221
  7. Ali MA, Abdelkader ESMA, El LMR. Interleukin-17 as a predictor of sepsis in polytrauma patients : a prospective cohort study. Eur J Trauma Emerg Surg [Internet]. 2017; Available from: http://dx.doi.org/10.1007/s00068-017-0841-3
  8. Faraj M, Salem N. C-Reactive Protein. In: Blood Cell- An Overview of Studies in Hematology. 2012. p. 89–100. Available from: https://www.intechopen.com/chapters/39106 doi: 10.5772/47735
  9. Yang Y, Xie J, Guo F, Longhini F, Gao Z, Huang Y, et al. Combination of C ‑ reactive protein , procalcitonin and sepsis ‑ related organ failure score for the diagnosis of sepsis in critical patients. Ann Intensive Care [Internet]. 2016;6(51). Available from: https://doi.org/10.1186/s13613-016-0153-5
  10. Pradhan S, Ghimire A, Bhattarai B, Khanal B, Pokharel K. The role of C ‑ reactive protein as a diagnostic predictor of sepsis in a multidisciplinary Intensive Care Unit of a tertiary care center in Nepal. Indian J Crit Care Med [Internet]. 2016;(41):417–20. Available from: https://doi.org/10.4103/0972-5229.186226
  11. Ranzani OT, Zampieri FG, Forte DN, Azevedo LCP, Park M. C-Reactive Protein/Albumin Ratio Predicts 90-Day Mortality of Septic Patients. PLoS One [Internet]. 2013;8(3):e59321. Available from: https://doi.org/10.1371/journal.pone.0059321
  12. Li Q, Gong X. Clinical significance of the detection of procalcitonin and C-reactive protein in the intensive care unit. Exp Ther Med [Internet]. 2018;15(5):4265–70. Available from: https://doi.org/10.3892/etm.2018.5960
  13. Akin H, Akalin H, Budak F, Ener B, Ocakoğlu G, Gürcüoğlu E, et al. Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia. Med Mycol [Internet]. 2015;53(3):258–68. Available from: https://doi.org/10.1093/mmy/myu084
  14. Zou Y, Chen X, Liu J, Zhou D bo, Kuang X, Xiao J, et al. Serum IL-1β and IL-17 levels in patients with COPD: association with clinical parameter. Int J Chron Obstruct Pulmon Dis [Internet]. 2017;12:1247–54. Available from: https://doi.org/10.2147/COPD.S131877
  15. Al-Saadany HM, Hussein MS, Gaber RA, Zaytoun HA. Th-17 cells and serum IL- 17 in rheumatoid arthritis patients: Correlation with disease activity and severity. Egypt Rheumatol [Internet]. 2016;38(1):1–7. Available from: http://dx.doi.org/10.1016/j.ejr.2015.01.001
  16. Ge Y, Huang M, Yao YM. Biology of Interleukin-17 and Its Pathophysiological Significance in Sepsis. Front Immunol [Internet]. 2020;11(July):1–13. Available from: https://doi.org/10.3389/fimmu.2020.01558
  17. Marchesi V V, Rukavina T. Interleukin-17 in experimental Klebsiella sepsis. Period Biol [Internet]. 2011;113(1):99–102. Available from: https://hrcak.srce.hr/67272
  18. Ogiku M, Kono H, Hara M, Tsuchiya M, Fujii H. Interleukin-17A Plays a Pivotal Role in Polymicrobial Sepsis According to Studies Using IL-17A Knockout Mice. J Surg Res [Internet]. 2012;174(1):142–9. Available from: http://dx.doi.org/10.1016/j.jss.2010.11.901
  19. Martins EC, Da Fe Silveira L, Viegas K, Beck AD, Júnior GF, Cremonese RV, et al. Neutrophil-lymphocyte ratio in the early diagnosis of sepsis in an intensive care unit: A case-control study. Rev Bras Ter Intensiva [Internet]. 2019;31(1):63–70. Available from: https://doi.org/10.5935/0103-507X.20190010
  20. Saǧ S, Saǧ MS, Tekeoǧlu I, Kamanll A, Nas K, Acar BA. Relationship of hematologic markers with IL-17 and IL-1 beta in patients with rheumatoid arthritis. J Back Musculoskelet Rehabil [Internet]. 2018;31(4):703–7. Available from: https://doi.org/10.3233/BMR-170903
  21. Delano MJ, Ward PA. Sepsis-induced immune dysfunction: Can immune therapies reduce mortality? J Clin Invest [Internet]. 2016;126(1):23–31. Available from: https://doi.org/10.1172/JCI82224
  22. Colo-Brunialti MK, Santos MC, Rigato O, Machado FR, Silva E, Salomao R. Increased Percentages of T Helper Cells Producing IL-17 and Monocytes Expressing Markers of Alternative Activation in Patients with Sepsis. PLoS ONE [Internet]. 7(5): e37393. Available from: https://doi.org/10.1371/journal
  23. pone.0037393
  24. Liestiadi DEF, Azlin E, Nafianti S. A hematologic scoring system and C-reactive protein compared to blood cultures for diagnosing bacterial neonatal sepsis. Paediatr Indones [Internet]. 2017;57(2):70–5. Available from: https://doi.org/10.14238/pi57.2.2017.70-5

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