DOI: https://doi.org/10.14710/jbtr.v7i3.12555

The Effectivity of Parsley (Petroselinum crispum) Extract on The Growth Inhibition of Candida Albicans

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 22 Oct 2021; Revised: 24 Dec 2021; Accepted: 27 Dec 2021; Available online: 31 Dec 2021; Published: 31 Dec 2021.
Open Access Copyright (c) 2021 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Denture stomatitis is a type of Candida-associated infection that mainly affects the palatal mucosa. Candida albicans is one of the normal flora that is considered to be the primary etiologic agent in the pathogenesis of denture stomatitis. Denture decontamination is necessary to prevent denture stomatitis. One method of decontamination is by immersing removable dentures in an antifungal solution. Parsley (Petroselinum crispum) is a medicinal plant showing antifungal activity. Objectives: To determine the effectivity of immersion of acrylic resin Plate in parsley extract on inhibition of growth of Candida albicans. 

Methods: The effectivity of immersion of heat-cured acrylic resin plates in parsley extract on inhibition of C. albicans growth was tested using an experimental laboratory study with a post-test-only control group design. Thirty samples were divided into 5 groups as 0.01%, 0.02%, and 0.05% parsley ethanol extract, 0.1% sodium hypochlorite, and sterile aquadest.  The number of colony forming units per mL was obtained after calculating the colonies on SDA media, allowing the minimum inhibitory concentration (MIC) obtained according to the formula. 

Results: 0.01% parsley ethanol extract is MIC with a mean of 7.4 CFU / ml, which inhibited the growth of C. albicans by 31.05%. The Kruskal Wallis test (p<0.001) results indicate that there is an effectivity of immersion of acrylic resin plate in parsley extract on inhibition of growth of C. albicans. Conclusions: The immersion of acrylic resin plates in parsley ethanol extract with a concentration of 0.01%, 0.02%, and 0.04% has effectivity on the inhibition of growth of C. albicans.

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Keywords: Parsley extract; Candida albicans; removable denture cleaning solution; MIC.

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Section: Original Research Articles
Language : EN
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  11. Gondim, B. L. C. et al. Effect of chitosan nanoparticles on the inhibition of Candida spp. biofilm on denture base surface. Arch. Oral Biol. 94, 99–107 (2018)
  12. An, S., Evans, J. L., Hamlet, S. & Love, R. M. Incorporation of antimicrobial agents in denture base resin : A systematic review. J. Prosthet. Dent. 1–8
  13. Arruda, C. N. F. de et al. Effect of sodium hypochlorite and Ricinus communis solutions on control of denture biofilm: A randomized crossover clinical trial. J. Prosthet. Dent. 117, 729–734 (2017)
  14. Puspitasari, D., Saputera, D. & Anisyah, R. N. Perbandingan Kekerasan Resin Akrilik Tipe Heat Cured Pada Perendaman Larutan Desinfektan Alkalin Peroksida Dengan Ekstrak Seledri (Apium Graveolens L.) 75%). ODONTO Dent. J. 3, 34 (2016)
  15. Deyab, M. H., Awady, B. E. & Bakir, N. G. Is immersion in mint oil or apple vinegar solution a valid antifungal approach for acrylic soft liners? Futur. Dent. J. 4, 302–307 (2018)
  16. Derouich, M. et al. Assessment of total polyphenols, flavonoids and anti-inflammatory potential of three Apiaceae species grown in the Southeast of Morocco. Sci. African 9, e00507 (2020)
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  18. Quan, Z. et al. Development and validation of a probe-based qPCR method to prevent parsley leaf material misidentification. Fitoterapia 146, 104666 (2020)
  19. Saleh, A. M., Selim, S., Jaouni, S. Al & AbdElgawad, H. CO 2 enrichment can enhance the nutritional and health benefits of parsley (Petroselinum crispum L.) and dill (Anethum graveolens L.). Food Chem. 269, 519–526 (2018)
  20. Jin, Y. S. Recent advances in natural antifungal flavonoids and their derivatives. Bioorganic Med. Chem. Lett. 29, (2019)
  21. Slimestad, R., Fossen, T. & Brede, C. Flavonoids and other phenolics in herbs commonly used in Norwegian commercial kitchens. Food Chem. 309, 125678 (2020)
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  23. Calderone, R. Candida Species Methods and Protocols. (Springer, 2016)
  24. Rahman, E. Efektivitas Ekstrak Daun Dewa (Gynura pseudochina (Lour.) DC) terhadap Pertumbuhan Candida albicans pada Plat Dasar Gigi Tiruan Resin Akrilik. Fak. Kedokt. Gigi UNISSULA 1–13 (2021)
  25. Abdu, K. & Hauwa, D. In vitro evaluation of the antioxidant and antimicrobial activity of leaf extracts of Petroselinum cripsum Parsley). ChemSearch J. 10, 25–29 (2019)
  26. Pineda, R., Vizcaíno, S., García, C. M., Gil, J. H. & Durango, D. Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum. Rev. Fac. Nac. Agron. Medellin 71, 8563–8572 (2018)
  27. Sahrul, G. Uji Daya Hambat Ekstrak Minyak Atsiri Kayu Manis (Cinnamomun burmanii) terhadap Pertumbuhan Jamur Malassezia furfur. JUSINDO 2507, 1–9 (2020)
  28. Agyare, C., Appiah, T., Boakye, Y. D. & Apenteng, J. A. Petroselinum crispum: A Review. Medicinal Spices and Vegetables from Africa: Therapeutic Potential Against Metabolic, Inflammatory, Infectious and Systemic Diseases (Elsevier Inc., 2017)
  29. Liberal, Â. et al. Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars. Molecules 25, (2020)
  30. Purwati, S., Lumora, S. V. T. & Samsurianto. Skrining Fitokimia Daun Saliara (Lantana camara L) Sebagai Pestisida Nabati Penekan Hama dan Insidensi Penyakit Pada Tanaman Holtikultura di Kalimantan Timur. Pros. Semin. Nas. Kim. 2017 153–158 (2017)
  31. Aboody, M. S. Al & Mickymaray, S. Anti-fungal efficacy and mechanisms of flavonoids. Antibiotics 9, (2020)
  32. Lee, H., Woo, E. R. & Lee, D. G. Apigenin induces cell shrinkage in Candida albicans by membrane perturbation. FEMS Yeast Res. 18, (2018)
  33. Aishwaya, J. Nutraceuticals Potential of Petroselinum Crispum: A Review. J. Complement. Med. Altern. Healthc. 7, 1–6 (2018)
  34. Daradkeh, G., Corporation, H. M. & Musthafa, M. E. Book chapter - parsley. (2018)
  35. Komala, O., Yulianita & Fuji, R. S. Activity Of Antifungal Ethanol Extract 50% And Ethanol 96% Leaf Henna Nail (Lawsonia inermis L) Against Trichophyton mentagrophytes. Ekol. J. Ilm. Ilmu Dasar dan Lingkung. Hidup 19, 12–19 (2019)

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