DOI: https://doi.org/10.14710/jvsar.v0i0.26556

Optimization Extraction of Anthocyanin from Roselle as a Natural Colorant using Natural Deep Eutectic Solvent with Microwave Assisted Extraction Method

1Departement of Industrial Technology, Universitas Diponegoro, Jl. Gubernur Mochtar, Tembalang, Semarang, Indonesia 50275, Indonesia

2Departement of Chemical Engineering, Universitas Diponegoro, Jl. Prof. Soedarto SH, Semarang, Indonesia 50275, Indonesia

Received: 10 Apr 2025; Revised: 11 Jun 2025; Accepted: 12 Jun 2025; Available online: 18 Jun 2025; Published: 30 Jun 2025.
Open Access Copyright (c) 2025 by Authors, Published by Vocational College of Universitas Diponegoro under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Abstract
This study aims to extracted of roselle using Microwave Assisted Extraction (MAE) with Natural Deep Eutectic Solvent (NADES) combination of sodium acetate and glycerin then examining its effect on the optimum anthocyanin content. The variables were applied using microwave assisted extraction power (200; 400; 600 watt), and extraction time (4; 6; 8 minutes). The variables were optimized using the response surface methodology (RSM) of 12 runs. The analysis includes antioxidant content (anthocyanins, vitamin C, tannins, and total phenolics) and stability tests (density and viscosity). The RSM results have shown that the optimization value of anthocyanin content is 0.3217%, which will be achieved at a extraction time of 6.02 minutes, and microwave assisted extraction power at 323.77 watt. The result of running the optimum anthocyanin variable shows anthocyanin content is 0.3072%. Microwave assisted extraction power and extraction time shown a significant effect on the antioxidant content.
Fulltext
Keywords: Roselle; Anthocyanin; NADES; MAE; Response Surface Methodology

Article Metrics:

Article Info
Section: Articles
Language : EN
Recent articles
Optimization Extraction of Anthocyanin from Roselle as a Natural Colorant using Natural Deep Eutectic Solvent with Microwave Assisted Extraction Method Optimization of Xanthone Extraction From Mangosteen Peel Using Natural Deep Eutentic Solvent and Microwave Assisted Extraction for Anti-Aging More recent articles
  1. Bajkacz, S., & Adamek, J. (2018). Development of a Method Based on Natural Deep Eutectic Solvents for Extraction of Flavonoids from Food Samples. Food Analytical Methods, 11(5), 1330–1344. https://doi.org/10.1007/s12161-017-1118-5
  2. Cannavacciuolo, C., Pagliari, S., Frigerio, J., Giustra, C.M., Labra, M., Campone, L., (2023), Natural Deep Eutectic Solvents (NADESs) Combined with Sustainable Extraction Techniques: A Review of the Green Chemistry Approach in Food Analysis, Trends Food Science Technology., 134, pp. 80-97
  3. Chatepa, L. E. C., Masamba, K. G., Sanudi, T., Ngwira, A., Tanganyika, J., & Chamera, F. (2023). Effects of aqueous and methanolic solvent systems on phytochemical and antioxidant extraction from two varieties of Roselle (Hibiscus sabdariffa L.) var. sabdariffa plant from Central Malawi. Food and Humanity, 1(September), 1172–1179. https://doi.org/10.1016/j.foohum.2023.09.006
  4. Fauziati, & Sampepana, E. (2016). Pemanfaatan Ekstrak Bunga Rosella sebagai Bahan Pewarna pada Produk Kacang Goyang. Jurnal Riset Teknologi Industri, 10(1), 78–87. https://doi.org/10.26578/jrti.v10i1.2071
  5. Herdiani, N., & Wikurendra, E. A. (2020). Aktivitas Antioksidan Ekstrak Kelopak Rosella (Hibiscus Sabdariffa Linn.) Dengan Metode DPPH. Prosiding Seminar Nasional Hasil Penelitian dan Pengabdian Masyarakat dengan tema “Kesehatan Modern dan Tradisional,” 214–219. http://repository.unusa.ac.id/6553/
  6. Imtiaz, F., Saif, Z., Sajid, A., Nazir, A., Manzoor, Q., Saleem, A., Mehr-un-Nisa, Farooq, A., Al-Mijalli, S. H., & Iqbal, M. (2024). Role of glycerol-based deep eutectic solvents for extraction of phytochemicals from Cichorium intybus seeds: Optimization by response surface methodology. Microchemical Journal, 199(February). https://doi.org/10.1016/j.microc.2024.110083
  7. Lellis, B., Fávaro-Polonio, C.Z., Pamphile, J.A., Polonio, J.C., (2020), Effects of textile dyes on health and the environment and bioremediation potential of living organisms, Environ Sci Pollut Res, 27(5), pp. 4552-4573
  8. Nguyen, M. P. (2020). Mircowave-Assisted Extraction of Phytochemical Constituents in Roselle (Hibiscus sabdariffa L.). Journal of Pharmaceutical Research International, 32(2), 1–12. https://doi.org/10.9734/jpri/2020/v32i230397
  9. Purbowati, I. S. M., & Maksum, A. (2019). The antioxidant activity of Roselle (Hibiscus sabdariffa Linii) phenolic compounds in different variations microwave-Assisted extraction time and power. IOP Conference Series: Earth and Environmental Science, 406(1). https://doi.org/10.1088/1755-1315/406/1/012005
  10. Puspita, F., Nurhidayati, I., Mellisani, B., Putri, F. A. R., & Prihadi, A. R. (2023). Aplikasi Natural Deep Eutectic Solvent (NADES) sebagai Green Solvent pada Ekstraksi Kafein dan Katekin dari Limbah Kulit Kopi. Pharmaceutical and Biomedical Sciences Journal (PBSJ), 5(1), 35–42. https://doi.org/10.15408/pbsj.v5i1.32256
  11. Rahmawati, S., Fauziah, L. A., Maiyulis, Ikhsan, & Hermansyah, O. (2022). Penetapan Kadar Vitamin C Buah Belimbing Wuluh Muda ( Averrhoa bilimbi L . ) dengan Metode Spektrofotometri UV-Vis. Jurnal Ilmu Kefarmasian, 3(2), 204–207
  12. Rosalinda, S., Azizah, I. W., & Nurjanah, S. (2023). Identifikasi Kadar Vitamin C Ekstrak Rosela (Hibiscus sabdariffa L.) Hasil Ekstraksi Berbantu Gelombang Mikro. Jurnal Teknotan, 17(3), 167. https://doi.org/10.24198/jt.vol17n3.2
  13. Sam, S., Malik, A., & Handayani, S. (2016). PENETAPAN KADAR FENOLIK TOTAL DARI EKSTRAK ETANOL BUNGA ROSELLA BERWARNA MERAH (Hibiscus sabdariffa L.) DENGAN MENGGUNAKAN SPEKTROFOTOMETRI UV-Vis. Jurnal Fitofarmaka Indonesia, 3(2), 182–187. https://doi.org/10.33096/jffi.v3i2.220
  14. Sari, T.S., Kusumawati, I., and Isnaeni, I., (2020), Color Stability and Antioxidant Activity of Red Roselle (Hibiscus Sabdariffa L.) Calyx Infuse, Journal Food Science Technology., 57(7), pp. 2546-2552
  15. Sun, R., Niu, Y., Li, M., Liu, Y., Wang, K., Gao, Z., Wang, Z., Yue, T., & Yuan, Y. (2023). Emerging trends in pectin functional processing and its fortification for synbiotics: A review. Trends in Food Science and Technology, 134(November 2022), 80–97. https://doi.org/10.1016/j.tifs.2023.03.004
  16. Suzery, M., Lestari, S., & Cahyono, B. (2018). Penentuan Total Antosianin dari Kelopak Bunga Rosela (Hibiscus sabdariffa L) dengan Metode Maserasi dan Sokhletasi. Jurnal Sains & Matematika (JSM), 18(1), 1–6
  17. Weremfo, A., Adulley, F., Adarkwah-Yiadom, M., (2020), Simultaneous Optimization of Microwave-Assisted Extraction of Phenolic Compounds and Antioxidant Activity of Avocado (Persea americana Mill.) Seeds Using Response Surface Methodology, Journal Food Science Technology., 57(4), pp. 1445-1456
  18. Yuan, Y., Zhang, J., Fan, J., Clark, J., Shen, P., Li, Y., & Zhang, C. (2018). Microwave assisted extraction of phenolic compounds from four economic brown macroalgae species and evaluation of their antioxidant activities and inhibitory effects on α-amylase, α-glucosidase, pancreatic lipase and tyrosinase. Food Research International, 113(May), 288–297. https://doi.org/10.1016/j.foodres.2018.07.021
  19. Yulistiani, F., Azzahra, R. K., & Nurhafshah, Y. A. (2020). Pengaruh Daya dan Waktu Terhadap Yield Hasil Ekstraksi Minyak Daun Spearmint Menggunakan Metode Microwave Assisted Extraction. Jurnal Teknik Kimia dan Lingkungan, 4(1), 1–6. https://doi.org/10.33795/jtkl.v4i1.127

Last update:

No citation recorded.

Last update:

No citation recorded.