Antioxidant Herbs Supplementation Inhibits Endometriosis Extension in Mice

*Yuli Trisetiyono orcid  -  Faculty of Medicine, Diponegoro University, Indonesia
Widjiati Widjiati orcid scopus  -  Faculty of Medicine, Airlangga University, Indonesia
Syarief Thaufik Hidayat orcid scopus  -  Faculty of Medicine, Diponegoro University, Indonesia
Noor Pramono  -  Faculty of Medicine, Diponegoro University, Indonesia
Received: 24 Apr 2019; Revised: 15 Oct 2019; Accepted: 15 Oct 2019; Published: 31 Dec 2019; Available online: 31 Dec 2019.
Open Access
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Article Info
Section: Original Research Articles
Language: EN
Statistics: 659 258
Abstract

Background: Increased oxidative stress causes inflammation and increases angiogenesis. It presumed to promote the proliferation of endometriosis tissue. Kebar grass (Biophytum petersianum) and other herbs such as green tea and Cucumis melo, which contain high antioxidants, are expected to decrease oxidative stress, inflammation, angiogenesis, and reduced endometriosis implants.

Objective: To investigate the effects of Kebar grass, green tea, and Cucumis melo to malondialdehyde serum, tumor necrosis factor alpha, and vascular endothelial growth factor expression, and the area of the endometriotic implants.

Methods: Twenty-eight mice were divided into four groups, i.e., the first group of endometriosis mice was given Kebar grass extract; the second group was assigned green tea extract, the third group was given the combination of Cucumis melo extract–gliadin, and the last containing the untreated endometriosis mice as the control. Each treatment was given for 14 days. The data of MDA serum level, the area of the endometriotic implants, TNF-α, and VEGF expression were collected and analyzed.

Results: The MDA serum levels of the groups treated with Kebar grass extract, green tea extract, and Cucumis melo extract – gliadin were significantly lower (p=0.001) than the control group. TNF-α expression of the groups provided with each treatment also lower than the control groups (p=0.002). However, only the administration of the Cucumis melo extract–gliadin resulted in lower VEGF expression compare with the control (p=0.017). Finally, the area of the endometriotic implants of the mice models administered with each treatment was smaller than the control group (p=0.003).

Conclusion: Kebar grass as well as green tea and Cucumis melo–gliadin inhibits endometriotic implants extension by decreasing MDA serum and TNF-α expression.

Keywords: Biophytum petersianum; green tea; cucumis melo; endometriosis; antioxidant herbs

Article Metrics:

  1. Fritz MA, Speroff L. Endometriosis. In: Clinical gynecologic endocrinology and infertility. 8th ed. Philadelphia: Lippincott Williams & Wilkins; 2010. p. 1221–48.
  2. Sekhon LH, Agarwal A. Endometriosis and oxidative stress. In: Agarwal A, Aziz N, Rizk B, editors. Studies on women’s health. New York: Humana Press; 2013. p. 149–67.
  3. Kralickova M, Vetvicka V. Role of angiogenesis in endometriosis. Pathol Discov. 2016;4(1):1–5.
  4. Rocha ALL, Reis FM, Taylor RN. Angiogenesis and endometriosis. Obstet Gynecol Int. 2013;2013:1–8.
  5. Sourial S, Tempest N, Hapangama DK. Theories on the pathogenesis of endometriosis. Int J Reprod Med. 2014;2014:1–9.
  6. Grund EM, Kagan D, Tran CA, Zeitvogel A, Starzinski-Powitz A, Nataraja S, et al. Tumor necrosis factor-a regulates inflammatory and mesenchymal responses via mitogen-activated protein kinase, p38, and nuclear factor B in human endometriotic epithelial cells. Mol Pharmacol. 2008;73:1394–404.
  7. Sanchez G, Zubor P, Szunyogh N, Kajo K, Machalekova K, Biringer K, et al. [TNF-alpha serum levels in women with endometriosis: prospective clinical study]. Ces Gynekol. 2005;70(4):286–90.
  8. Harada T, Taniguchi F, Izawa M, Ohama Y, Takenaka Y, Tagashira Y, et al. Apoptosis and endometriosis. Front Biosci. 2007;12:3140–51.
  9. Fritz MA, Speroff L. Clinical Gynecologic Endocrinology and Infertility. 8th ed. Philadelphia: Lippincott Williams & Wilkins; 2011.
  10. Sadsoeitoeboen PD. [Benefits of Kebar Grass extract (Biophytum petersianum Klotzsch) on the reproductive appearance of white female mice]. Vol. Pasca Sarj. [Bogor]: Institut Pertanian Bogor; 2005.
  11. Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M. Herbal antioxidant in clinical practice: a review. Asian Pac J Trop Biomed. 2014;4(1):78–84.
  12. Sembiring B, Darwati I. [Identification of chemical components of accession of Kebar Grass (Biophytum petersianum) from Papua and Java]. Obat BPTR dan, editor. 2014;25(1):37–44.
  13. Sukarsono. [Profile of secondary metabolite content of medicinal plants Biophytum petersianum and Biophytum sensitivum]. J Gamma. 2012;6(1):69–76.
  14. Man GCW, Xu H, Wang CC. Green Tea for Endometriosis. Chaudhury K, Chakravarty B, editors. Endometriosis. IntechOpen; 2012.
  15. Romao S. Therapeutic value of oral supplementation with melonsuperoxide dismutase and wheat gliadin combination. Nutrition. 2015;31(3):430–6.
  16. Hendarto H, Widjiati, Johari S. [Curcumin supplementation to improve oocyte maturation and results of in vitro fertilization in endometriosis mice]. Maj Obstet Ginekol. 2014;22(2):53–7.
  17. Nowak M, Madej JA, Dziegiel P. Intensity of cox2 expression in cells of soft tissue fibrosacrcomas in dogs as related to grade of tumour malignancy. Bull Vet Inst Pulawy. 2007;51:275–9.
  18. Crosignani P, Olive D, Bergqvist A, Luciano A. Advances in the management of endometriosis: an update for clinicians. Hum Reprod Update. 2006;12(2):179–89.
  19. Gupta S, Chandra A, Kesavan S, Eapen D, Agarwal A. Oxidative Stress and the Pathogenesis of Endometriosis. In: Garcia-Velasco JA, Rizk BRMB, editors. Endometriosis: Current management and future trends. Cleveland: ClevelandClinic; 2010. p. 31–9.
  20. Xu H, Lui WT, Chu CY, Ng PS, Wang CC, Rogers MS. Anti-angiogenic effects of green tea catechin on an experimental endometriosis mouse model. Hum Reprod. 2009;24(3):608–18.
  21. Xu H, Becker CM, Lui WT, Chu CY, Davis TN, Kung AL, et al. Green tea epigallocatechin-3-gallate inhibits angiogenesis and suppresses vascular endothelial growth factor C/vascular endothelial growth factor receptor 2 expression and signaling in experimental endometriosis in vivo. Fertil Steril. 2011;96(4):1021–8.
  22. Laschke MW, Schwender C, Scheuer C, Vollmar B, Menger MD. Epigallocatechin-3-gallate inhibits estrogen-induced activation of endometrial cells in vitro and causes regression of endometriotic lesions in vivo. Hum Reprod. 2008;23(1):2308–18.
  23. Romao S. Therapeutic value of oral supplementation with melon superoxide dismutase and wheat gliadin combination. Nutrition. 2015;31(3):430–6.
  24. Jackson LW, Schisterman EF, Dey-Rao R, Browne R, Armstrong D. Oxidative Stress and Endometrosis. Hum Reprod. 2005;20(7):2014–20.
  25. Agarwal A, Gupta S, Agarwal A, Krajcir N, Alvarez JG. Role of oxidative stress in endometriosis. Reprod Biomed Online. 2006;13(1):126–34.
  26. Das K, Bhattacharyya J. Antioxidant Functions of Green and Black Tea. In: Preedy VR, editor. Tea in Health and Disease Prevention. London: Elsevier; 2013. p. 521–8.
  27. Santanam N, Kavtaradze N, Murphy A, Dominguez C, Parthasarathy S. Antioxidant supplementation reduces Eendometriosis related pelvic pain in humans. Transl Res J Lab Clin Med. 2014;161(3):189–95.
  28. Erten OU, Ensari T gba A, Dilbaz B, Cakiroglu H, Altinbas SK, Çaydere M, et al. Vitamin C is effective for the prevention and regression of endometriotic implants in an experimentally induced rat model of endometriosis. Taiwan J Obstet Gynecol. 2016;55:251–7.
  29. Tsuchiya M, Miura T, Hanaoka T, Iwasaki M, Sasaki H, Tanaka T, et al. Effect of soy isoflavones on endometriosis: interaction with estrogen receptor 2 gene polymorphism. Epidemiology. 2007;18(3):402–8.
  30. Menvielle-Bourg FJ. Superoxide dismutase (SOD), a powerful antioxidant, is now available orally. Phytothérapie. 2005;3:1–4.
  31. Panjaitan BC, Sa’adi A, Hendarto H, Widjiati. Comparison of ovarial malondialdehyde (MDA) level between endometriosis rat given with and without curcumine supplementation. Maj Obstet Ginekol. 2012;20(1):30–4.
  32. Sukina B, Prabowo GI, Suhartati S, Harianto N. [Green tea catechins (Camellia sinensis) against malondialdehyde and superoxide dismutase]. Indones J Clin Pathol Med Lab. 2013;19(2):92–7.
  33. Artika FN, Susetyowati, Pramantara IDP, Lestari LA. [Effect of green tea extract (Camellia sinensis) to plasma malondialdehyde (MDA) level and hand grip strength of elderly]. 2016;13(2):59–66.
  34. Suryadinata RV, Wirjatmadi B, Adriani M. Effectiveness decrease combined with supplements malondialdehyde antioxidant superoxide dismutase gliadin melon with due to exposure to cigarette. Glob Med Heal Commun. 2017;5(2):79–83.
  35. Greene AD, Lang SA, Kendziorski JA, Sroga-rios JM, Herzog TJ, Burns KA. Endometriosis : Where are We and Where are We Going ? 2017;152(3):1–29.
  36. Wu M-H, Tsai S-J. Endometriosis and possible inflammation markers. Gynecol Minim Invasive Ther. 2015;4(3):61–7.
  37. Rizzo MR, Abbatecola AM, Barbieri M, Vietri MT, Cioffi M, Grella R, et al. Evidence for anti-inflammatory effects of combined administration of vitamin E and C in older persons with impaired fasting glucose: impact on insulin action. J Am Coll Nutr. 2008;27(4):505–11.
  38. Desai SJ, Prickril B, Rasooly A. Mechanisms of phytonutrient modulation of Cyclooxygenase-2 (COX-2) and inflammation related to cancer. Nutr Cancer. 2018;70(3):350–75.
  39. Suganuma M, Takahashi A, Watanabe T, Iida K, Matsuzaki T, Yoshikawa HY, et al. Biophysical approach to mechanisms of cancer prevention and treatment with green tea catechins. Molecules. 2016;21(11):1–17.
  40. Chen Z, Lin Z. Tea and human health: biomedical functions of tea active components and current issues. J Zhejiang Univ B. 2015;16(2):87–102.
  41. Helieh SO. Chronic Inflammatory Diseases and Green Tea Polyphenols. Nutrients. 2017;9(6):561.
  42. Lin Y-H, Chen Y-H, Chang H-Y, Au H-K, Tzeng C-R, Huang Y-H. Chronic Niche Inflammation in Endometriosis-Associated Infertility: Current Understanding and Future Therapeutic Strategies. Int J Mol Sci. 2018;19(8):2385.
  43. Vouldoukis I, Lacan D, Kamate C, Coste P, Calenda A, Mazier D, et al. Antioxidant and anti-inflammatory properties of a Cucumis melo LC. extract rich in superoxide dismutase activity. J Ethnopharmacol. 2004;94(1):67–75.
  44. Laschke MW, Menger MD. Anti-angiogenic treatment strategies for the therapy of endometriosis. Hum Reprod Update. 2012;18(6):682–702.
  45. Schindler R, Mentlein† R. Flavonoids and Vitamin E Reduce the Release of the Angiogenic Peptide Vascular Endothelial Growth Factor from Human Tumor Cells. Journals Nutr. 2006;136:1477–2006.
  46. Granese R, Bitto A, Polito F, Triolo O, Giordano D, Santamaria A, et al. Genistein reduces angiogenesis and apoptosis in women with endometrial hyperplasia. Bot Targets Ther. 2014;5(5):27–32.
  47. Jehanara, Sutrisno, Santoso S. [Effect of Genistein on Decreasing Vascular Levels of Endothelial Growth Factor-A in Endometriosis Cell Culture]. Maj Obstet Ginekol. 2014;22(2):94–100.
  48. Krikun G. Endometriosis, Angiogenesis and Tissue Factor. Scientifica (Cairo). 2012;2012:1–10.
  49. Lizandi AO, Sardjono CT, Widowati W, Gunanegara RF, Sandra F. [GliSODin Increases Stem Cells Potency]. Cermin Dunia Kedokt. 2010;37(2):107–10.