DOI: https://doi.org/10.14710/jbtr.v5i2.4679

Effect of Superoxide Dismutase (SOD) Supplementation on Plasma Levels of Malondialdehyde (MDA), Total Cholesterol and LDL Cholesterol in the Elderly

Faculty of Medicine, Diponegoro University, Indonesia

Received: 13 Mar 2019; Revised: 10 Oct 2019; Accepted: 10 Oct 2019; Available online: 31 Dec 2019; Published: 31 Dec 2019.
Open Access Copyright (c) 2019 Journal of Biomedicine and Translational Research

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Abstract

Background: Several various physiological functions in elderly people are diminished due to cell or tissue damage. One of the probable causes are oxidative stress yielded by free radicals.

Oxidative stress (ROS) induce lipid peroxidation in endothelial cell membrane, which generates atherosclerotic plaque. In a state of oxidative stress, MDA level will increased. The purpose of this study is to determine the effect of SOD supplementation on MDA, total cholesterol and LDL cholesterol plasma levels in the elderly.

Methods: This study was open label, a randomized control trial. Subjects were elderly people aged > 60 years (median 75, 60-82 ys, male 10 (24,4%)) institutionalized at Social Rehabilitation Unit Pucang Gading Semarang, Indonesia. The treatment group consisted of 16 people, received SOD (GlisodinR) 1 capsule (250 IU) 1 hour before meals, plus exercise scheduled for 8 weeks. The control group consisted of 15 people, received placebo, and exercise. Plasma MDA levels were examined using TBARS method, while total cholesterol and LDL cholesterol were examined using CHOD-PAP method.

Results: This study show a reduction of plasma MDA levels in the treatment group compare to control group ( p = 0.062 ). A significant reduction of total cholesterol and LDL cholesterol levels in the treatment group were found (before 190.00 and 131.47 g/dl, after 182.27 and 121.93 g/dl, p = 0.005 and 0.001).

Conclusion: The SOD supplementation significantly reduce Total Cholesterol and LDL level, but not MDA level in the elderly.

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Keywords: SOD; MDA; Total Cholesterol; LDL Cholesterol

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Section: Original Research Articles
Language : EN
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  1. Harman. Aging : a Theory Based on Free Radical and Radiation Chemistry 1956;298–300
  2. Balaban RS, Nemoto S, Finkel T. Mitochondria, oxidants, and aging. Cell 2005;120(4):483–95
  3. Ayala A, Muñoz M, Argüelles S. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Med Cell 2014
  4. Mudau M, Genis A, Lochner A, Strijdom H. Endothelial dysfunction : the early predictor of atherosclerosis. Cardiovasc J Afr 2012;23(4):222–31
  5. Csiszar A, Ungvari Z, Edwards JG, Kaminski P, Wolin MS, Koller A, et al. Aging-induced phenotypic changes and oxidative stress impair coronary arteriolar function. Circ Res 2002;90(11):1159–66
  6. Marnett LJ. Lipid peroxidation—DNA damage by malondialdehyde. Mutat Res Mol Mech Mutagen 1999;424(1–2):83–95
  7. Perry JJP, Shin DS, Getzoff ED, Tainer JA. The structural biochemistry of the superoxide dismutases. Biochim Biophys Acta - Proteins Proteomics. Elsevier B.V. 2010;1804(2):245–62
  8. Nakajima S, Ohsawa I, Nagata K, Ohta S, Ohno M, Ijichi T, et al. Oral supplementation with melon superoxide dismutase extract promotes antioxidant defences in the brain and prevents stress-induced impairment of spatial memory. Behav Brain Res. Elsevier B.V. 2009;200:15–21
  9. Romao S. Therapeutic value of oral supplementation with melon superoxide dismutase and wheat gliadin combination. Nutrition [Internet]. Elsevier Inc 2015;31(3):430–6
  10. Xu L, Porter NA. Free radical oxidation of cholesterol and its precursors: Implications in cholesterol biosynthesis disorders. Free Radic Res 2015;49(7):835–49
  11. Cominacini L, Rigoni A, Pasini AF, Garbin U, Davoli A, Campagnola M, et al. The Binding of Oxidized Low Density Lipoprotein (ox-LDL) to ox-LDL Receptor-1 Reduces the Intracellular Concentration of Nitric Oxide in Endothelial Cells through an Increased Production of Superoxide. J Biol Chem 2001;276(17):13750–5
  12. Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors. Clin Chem 1997;43(7):1209–14
  13. Bose KSC, Agrawal BK. Effect of Long Term Supplementation of Tomatoes (Cooked) on Levels of Antioxidant Enzymes, Lipid Peroxidation Rate, Lipid Profile and Glycated Haemoglobin in Type 2 Diabetes Mellitus 2006;55(4):274–8
  14. Rajprabha, Hamid, Kumarmeena R, Syedyawer H. Study of Antioxidant Enzyme -Superoxide Dismutase Activity and Lipid Profile in Diabetes Mellitus patients. Int J Healthc Biomed Res 2014;2(4):22–9
  15. Décordé K, Ventura E, Lacan D, Ramos J, Cristol JP, Rouanet JM. An SOD rich melon extract Extramel®prevents aortic lipids and liver steatosis in diet-induced model of atherosclerosis. Nutr Metab Cardiovasc Dis 2010;20(5):301–7
  16. Carillon J, Rouanet JM, Cristol JP, Brion R. Superoxide dismutase administration, a potential therapy against oxidative stress related diseases: Several routes of supplementation and proposal of an original mechanism of action. Pharm Res 2013;30(11):2718–28
  17. Oliveras-López MJ, Molina JJM, Mir MV, Rey EF, Martín F, De la Serrana HLG. Extra virgin olive oil (EVOO) consumption and antioxidant status in healthy institutionalized elderly humans. Arch Gerontol Geriatr 2013;57(2):234–42
  18. Pourghassem-Gargari B, Ebrahimzadeh-Attary V, Rafraf M, Gorbani A. Effect of dietary supplementation with Nigella sativa L. on serum lipid profile, lipid peroxidation and antioxidant defense system in hyperlipidemic rabbits. J Med Plants Res 2009;3(10):815–21
  19. Hassan S, El-Twab SA, Hetta M, Mahmoud B. Improvement of lipid profile and antioxidant of hypercholesterolemic albino rats by polysaccharides extracted from the green alga Ulva lactuca Linnaeus. Saudi J Biol Sci [Internet]. King Saud University 2011;18(4):333–40
  20. Mondola P, Damiano S, Sasso A, Santillo M. The Cu, Zn superoxide dismutase: Not only a dismutase enzyme. Front Physiol 2016;7(NOV):1–8
  21. Zou Y, Corniola R, Leu D, Khan A, Sahbaie P, Chakraborti A, et al. Extracellular superoxide dismutase is important for hippocampal neurogenesis and preservation of cognitive functions after irradiation. Proc Natl Acad Sci USA 2012;109(52):21522–7
  22. Fattman CL, Schaefer LM, Oury TD. Extracellular superoxide dismutase in biology and medicine. Free Radic Biol Med 2003;35(3):236–56
  23. Cloarec M, Caillard P, Provost JC, Dever JM, Elbeze Y, Zamaria N. GliSODin, a vegetal sod with gliadin, as preventative agent vs. atherosclerosis, as confirmed with carotid ultrasound-B imaging. Eur Ann Allergy Clin Immunol 2007;39(2):45–50
  24. Skarpanska-Stejnborn A, Pilaczynska-Szczesniak L, Basta P, Deskur-Smielecka E, Woitas-Slubowska D, Adach Z. Effects of oral supplementation with plant superoxide dismutase extract on selected redox parameters and an inflammatory marker in a 2,000-m rowing-ergometer test. Int J Sport Nutr Exerc Metab 2011;21(2):124–34

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