DOI: https://doi.org/10.14710/jbtr.v12i2.28806

Retinal Thickness After Platelet Rich Plasma Injection in Glaucoma Model Rats

1Master Program in Departement of Biology, Faculty of Mathematics and Science, Syiah Kuala University, Indonesia

2Departemet of Biology, Faculty of Mathematics and Science, Syiah Kuala University, Banda Aceh, Indonesia

3Departement of Opthalmology, Faculty of Medicine, Syiah Kuala University, Banda Aceh, Indonesia

4 Departement of Neurology, Faculty of Medicine, Syiah Kuala University, Banda Aceh, Indonesia

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Received: 31 Jul 2025; Revised: 3 Apr 2026; Accepted: 14 Apr 2026; Available online: 29 Aug 2026; Published: 31 Aug 2026.
Open Access Copyright (c) 2026 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Platelet-rich plasma (PRP) is an autologous blood component that contains a high concentration of platelets. PRP is known contains growth factors that play roles in cell regeneration, proliferation, and differentiation. This study used a completely randomized design (CRD) consisting of 5 groups and 5 repetitions. The test animals used were 25 male Wistars rats aged 2 months. The animal model of glaucoma was given 250 mmHg pressure on the right eye for 60 minutes and PRP was given 7 days after the pressure was increased. Then the thickness of the eye retina was observed after 8 weeks. The eye of the test animals were collected and made into histology preparations using paraffin method and Hematoxylin Eosin (HE) staining. Data were analyzed using ANOVA test with a significant level of 5% and continued with Duncan’s multiple tests. The result showed that there was a significant effect of PRP injection on the retinal thickness in the inner plexiform layer. Intravitreal-intraperitoneal injection of PRP is an effective injection route because it is capable of repairing damage based on the results of the thickness of the inner plexiform layer.

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Keywords: Platelet Rich Plasma (PRP) Injection; Glaucoma; Intraocular Pressure; Retinal Thickness; Caspase-3
Funding: Institute of Research and Public Service Syiah Kuala University

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Section: Original Research Articles
Language : EN
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