DOI: https://doi.org/10.14710/jbtr.v11i1.24127

Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats

1Ophthalmology Department, Faculty of Medicine, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

2Anatomical Pathology Department, Faculty of Medicine, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

Received: 8 Aug 2024; Revised: 24 Apr 2025; Accepted: 21 Apr 2025; Available online: 30 Apr 2025; Published: 30 Apr 2025.
Open Access Copyright (c) 2025 Journal of Biomedicine and Translational Research
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Abstract

Background: Elevated intraocular pressure (IOP) induces inflammation in the trabecular meshwork (TM) through increases in tumor necrosis factor-alpha (TNF-α) expressions by activating nuclear factor kappa B (NF-κB). Mechanical injury to the TM, such as increased IOP, can cause extracellular matrix (ECM) remodeling, impacting the permanent outflow of aqueous humor. Moringa oleifera (MO) leaf extract is an anti- inflammatory agent that inhibits NF-κB activation and has been studied to have antioxidant effects.
Objective: to analyze the effects of MO leaf extract on TNF- α expression and the thickness of the trabecular meshwork ECM in a Wistar rat glaucoma model. Methods: Fourteen male Wistar rats were divided into two groups (n=7): the glaucoma group received oral MO leaf extract at 300 mg/kg body weight for four weeks, and the control group. TNF-α expressions were measured by immunohistochemical stain, and the thickness of the ECM was examined with hematoxylin eosin staining. P-values of less than 0.05 were considered to represent statistical significance.
Results: The mean of TNF-α expressions in the treatment group was 4.29 ± 0.76, and the control group was 5.29 ± 0.49. There was a significant difference in TNF-α expressions between the two groups (p=0.015). The mean thickness of the ECM TM experimental group and control group were 155.65 ± 28.72μm and 218.63 ± 40.85μm respectively. The extracellular matrix TM experimental group was statistically significantly thinner than the control group (p=0.006).
Conclusion: MO could protect TM from damage caused by high IOP by suppressed TNF- α expression and ECM thickness in the TM Wistar glaucoma model.

 

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Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats
Subject Moringa oleifera, TNF-α, trabecular meshwork, glaucoma
Type Cover Letter
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Keywords: Moringa oleifera, TNF-α, trabecular meshwork, glaucoma

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Section: Original Research Articles
Language : EN
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