DOI: https://doi.org/10.14710/jbtr.v11i1.24127

Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats

1Ophthalmology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Anatomical Pathology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 8 Aug 2024; Revised: 24 Apr 2025; Accepted: 21 Apr 2025; Available online: 30 Apr 2025; Published: 30 Apr 2025.
Open Access Copyright (c) 2025 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to cite (Vancouver): Maharani M, Astuti RK, Rahmi FL, Prihatningtias R, Istiadi H, Wildan A. Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats. Journal of Biomedicine and Translational Research [Online]. 2025 Apr;11(1). https://doi.org/10.14710/jbtr.v11i1.24127.
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Abstract

Background: Elevated intraocular pressure (IOP) induces inflammation in the trabecular meshwork (TM) through increases in tumor necrosis factor-alpha (TNF-α) expressions by activating nuclear factor kappa B (NF-κB). Mechanical injury to the TM, such as increased IOP, can cause extracellular matrix (ECM) remodeling, impacting the permanent outflow of aqueous humor. Moringa oleifera (MO) leaf extract is an anti- inflammatory agent that inhibits NF-κB activation and has been studied to have antioxidant effects.
Objective: to analyze the effects of MO leaf extract on TNF- α expression and the thickness of the trabecular meshwork ECM in a Wistar rat glaucoma model. Methods: Fourteen male Wistar rats were divided into two groups (n=7): the glaucoma group received oral MO leaf extract at 300 mg/kg body weight for four weeks, and the control group. TNF-α expressions were measured by immunohistochemical stain, and the thickness of the ECM was examined with hematoxylin eosin staining. P-values of less than 0.05 were considered to represent statistical significance.
Results: The mean of TNF-α expressions in the treatment group was 4.29 ± 0.76, and the control group was 5.29 ± 0.49. There was a significant difference in TNF-α expressions between the two groups (p=0.015). The mean thickness of the ECM TM experimental group and control group were 155.65 ± 28.72μm and 218.63 ± 40.85μm respectively. The extracellular matrix TM experimental group was statistically significantly thinner than the control group (p=0.006).
Conclusion: MO could protect TM from damage caused by high IOP by suppressed TNF- α expression and ECM thickness in the TM Wistar glaucoma model.

 

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Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats
Subject Moringa oleifera, TNF-α, trabecular meshwork, glaucoma
Type Cover Letter
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Keywords: Moringa oleifera, TNF-α, trabecular meshwork, glaucoma

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Section: Original Research Articles
Language : EN
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  5. Jia Y, Jiang S, Chen C, Lu G, Xie Y, Sun X, et al. Caffeic acid phenethyl ester attenuates nuclear factor‑κB‑mediated inflammatory responses in Muller cells and protects against retinal ganglion cell death. Molecular Medicine Reports. 2019;9(10):1013. https://doi.org/10.3892/mmr.2019.10151
  6. Buonfiglio F, Pfeiffer N, Gericke A. Immunomodulatory and Antioxidant Drugs in Glaucoma Treatment. Pharmaceuticals. 2023;16(9):1193. https://doi.org/10.3390/ph16091193
  7. Vranka JA, Kelley MJ, Acott TS, Keller KE. Extracellular matrix in the trabecular meshwork: Intraocular pressure regulation and dysregulation in glaucoma. Experimental Eye Research. 2015;133:112–25. https://doi.org/10.3390/ijms24032098
  8. Keller KE, Peters DM. Pathogenesis of glaucoma: Extracellular matrix dysfunction in the trabecular meshwork‐A review. Clinical & Experimental Ophthalmology. 2022;50(2):163–82. https://doi.org/10.1111/ceo.14027
  9. Pareek A, Pant M, Gupta MM, Kashania P, Ratan Y, Jain V, et al. Moringa oleifera: An Updated Comprehensive Review of Its Pharmacological Activities, Ethnomedicinal, Phytopharmaceutical Formulation, Clinical, Phytochemical, and Toxicological Aspects. International Journal of Molecular Sciences. 2023;24(3):2098. https://doi.org/10.3390/ijms24032098
  10. Siahaan SCP, Santoso B, Widjiati -. Effectiveness of Moringa oleifera Leaves on TNF-α Expression, Insulin Levels, Glucose Levels and Follicle Count in Rattus norvegicus PCOS Model. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 2022;Volume 15:3255–70. https://doi.org/10.2147/DMSO.S385492
  11. Kashyap P, Kumar S, Riar CS, Jindal N, Baniwal P, Guiné RPF, et al. Recent Advances in Drumstick (Moringa oleifera) Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary Applications. Antioxidants. 2022;11(2):402. https://doi.org/10.3390/antiox11020402
  12. Kou X, Li B, Olayanju J, Drake J, Chen N. Nutraceutical or Pharmacological Potential of Moringa oleifera Lam. Nutrients. 2018;10(3):343. https://doi.org/10.3390/nu10030343
  13. Ahmad FMF, Wan Ahmad WSHM, Jamaluddin MF, Lee JTH, Khor SY, Looi LM, et al. Allred Scoring of ER-IHC Stained Whole-Slide Images for Hormone Receptor Status in Breast Carcinoma. Diagnostics (Basel, Switzerland). 2022;12(12). https://doi.org/10.3390/antiox11020402
  14. Biswas S, Wan KH. Review of rodent hypertensive glaucoma models. Acta Ophthalmologica. 2019;97(3):e331–40. https://doi.org/10.1111/aos.13983
  15. Vernazza S, Tirendi S, Bassi AM, Traverso CE, Saccà SC. Neuroinflammation in Primary Open-Angle Glaucoma. Journal of clinical medicine. 2020;9(10). https://doi.org/10.3390/jcm9103172
  16. Dammak A, Sanchez Naves J, Huete-Toral F, Carracedo G. New Biomarker Combination Related to Oxidative Stress and Inflammation in Primary Open-Angle Glaucoma. Life. 2023;13(7):1455. https://doi.org/10.3390/life13071455
  17. Sr. YZ, Yang X, Li Y, Bai Y, Ge J. TNF-α,TNFR1 And NFB Expression In Trabecular Meshwork Cells of Primary Open Angle Glaucoma. Investigative Ophthalmology & Visual Science. 2011;52(14):4606. https://doi.org/10.3390/jcm9103172
  18. Goel M, Picciani RG, Lee RK, Bhattacharya SK. Aqueous humor dynamics: a review. The open ophthalmology journal. 2010;4:52–9. https://doi.org/10.2174/1874364101004010052
  19. Grozdanic SD, Betts DM, Sakaguchi DS, Kwon YH, Kardon RH, Sonea IM. Temporary elevation of the intraocular pressure by cauterization of vortex and episcleral veins in rats causes functional deficits in the retina and optic nerve. Experimental Eye Research. 2003;77(1):27–33. https://doi.org/10.1016/S0014-4835(03)00089-7
  20. Bai Y, Zhu Y, Chen Q, Xu J, Sarunic M V, Saragovi UH, et al. Validation of glaucoma-like features in the rat episcleral vein cauterization model. Chinese Medical Journal. 2014;127(2). https://doi.org/10.3760/cma.j.issn.0366-6999.20132398
  21. Roh M, Zhang Y, Murakami Y, Thanos A, Lee SC, Vavvas DG, et al. Etanercept, a widely used inhibitor of tumor necrosis factor-α (TNF- α), prevents retinal ganglion cell loss in a rat model of glaucoma. PLoS ONE. 2012;7(7):1–13. https://doi.org/10.1371/journal.pone.0040065
  22. Srivastava S, Pandey VK, Dash KK, Dayal D, Wal P, Debnath B, et al. Dynamic bioactive properties of nutritional superfood Moringa oleifera: A comprehensive review. Journal of Agriculture and Food Research. 2023;14(2):100860. https://doi.org/10.1016/j.jafr.2023.100860
  23. George GO, Ajayi OB, Oyemike AA. Effect of Moringa oleifera leaf aqueous extract on intraocular and blood pressure of normotensive adults in Edo State, Nigeria. In 2018. https://doi.org/10.4314/JNOA.V20I2
  24. Chiș A, Noubissi PA, Pop OL, Mureșan CI, Fokam Tagne MA, Kamgang R, et al. Bioactive Compounds in Moringa oleifera: Mechanisms of Action, Focus on Their Anti-Inflammatory Properties. Plants. 2024;13(1). https://doi.org/10.3390/plants13010020
  25. Morgan MJ, Liu Z gang. Crosstalk of reactive oxygen species and NF-κB signaling. Cell Research. 2011;21(1):103–15. https://doi.org/10.1038/cr.2010.178
  26. Aly O, Abouelfadl DM, Shaker OG, Hegazy GA, Fayez AM, Zaki HH. Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats. Egyptian Journal of Medical Human Genetics. 2020;21(1):69. https://doi.org/10.1186/s43042-020-00106-z
  27. Wahyu PF, Widiartini C. The Effect Of Kelor Leaves (Moringa oleifera) Ethanol Extract On Serum Uric Acid And Tumor Necrosis Factor-α Of Hyperuricemic White Rats (Rattus norvegicus). IOP Conference Series: Earth and Environmental Science. 2019;406(1):012006. https://doi.org/10.1088/1755-1315/406/1/012006
  28. Kumar GS, Kumar B, Srinivasan BP, Nag TC, Srivastava S, Saxena R, et al. Retinoprotective Effects of Moringa oleifera via Antioxidant, Anti-Inflammatory, and Anti-Angiogenic Mechanisms in Streptozotocin-Induced Diabetic Rats. Journal of Ocular Pharmacology and Therapeutics. 2013;29(4):419–26. https://doi.org/10.1089/jop.2012.0089
  29. Joshua O, Sunday O, John OA, Temitope I. Exploring Moringa Oleifera Usefulness In Ameliorating Induced Retinopathy In Experimental Animals. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS. 2017;12(5):21–9. https://doi.org/10.9790/3008-1205032129
  30. Caban M, Owczarek K, Lewandowska U. The Role of Metalloproteinases and Their Tissue Inhibitors on Ocular Diseases: Focusing on Potential Mechanisms. International Journal of Molecular Sciences. 2022;23(8). https://doi.org/10.1088/1755-1315/406/1/012006

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