DOI: https://doi.org/10.14710/jbtr.v11i1.24127

Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats

1Ophthalmology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

2Anatomical Pathology Department, Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 8 Aug 2024; Revised: 24 Apr 2025; Accepted: 21 Apr 2025; Available online: 30 Apr 2025; Published: 30 Apr 2025.
Open Access Copyright (c) 2025 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Elevated intraocular pressure (IOP) induces inflammation in the trabecular meshwork (TM) through increases in tumor necrosis factor-alpha (TNF-α) expressions by activating nuclear factor kappa B (NF-κB). Mechanical injury to the TM, such as increased IOP, can cause extracellular matrix (ECM) remodeling, impacting the permanent outflow of aqueous humor. Moringa oleifera (MO) leaf extract is an anti- inflammatory agent that inhibits NF-κB activation and has been studied to have antioxidant effects.
Objective: to analyze the effects of MO leaf extract on TNF- α expression and the thickness of the trabecular meshwork ECM in a Wistar rat glaucoma model. Methods: Fourteen male Wistar rats were divided into two groups (n=7): the glaucoma group received oral MO leaf extract at 300 mg/kg body weight for four weeks, and the control group. TNF-α expressions were measured by immunohistochemical stain, and the thickness of the ECM was examined with hematoxylin eosin staining. P-values of less than 0.05 were considered to represent statistical significance.
Results: The mean of TNF-α expressions in the treatment group was 4.29 ± 0.76, and the control group was 5.29 ± 0.49. There was a significant difference in TNF-α expressions between the two groups (p=0.015). The mean thickness of the ECM TM experimental group and control group were 155.65 ± 28.72μm and 218.63 ± 40.85μm respectively. The extracellular matrix TM experimental group was statistically significantly thinner than the control group (p=0.006).
Conclusion: MO could protect TM from damage caused by high IOP by suppressed TNF- α expression and ECM thickness in the TM Wistar glaucoma model.

 

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Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats
Subject Moringa oleifera, TNF-α, trabecular meshwork, glaucoma
Type Cover Letter
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Keywords: Moringa oleifera, TNF-α, trabecular meshwork, glaucoma

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Section: Original Research Articles
Language : EN
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