DOI: https://doi.org/10.14710/jbtr.v7i2.11686

The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 9 Jul 2021; Revised: 18 Aug 2021; Accepted: 18 Aug 2021; Available online: 31 Aug 2021; Published: 31 Aug 2021.
Open Access Copyright (c) 2021 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

ABSTRACT

Background: Obesity is a disorder or disease characterized by the accumulation of excess fat in the body due to an imbalance in energy intake that is used for a long time. Accumulation of fat can reduce adiponectin, causing cardiac hypertrophy, endothelial vasodilation, and other cardiovascular diseases. Black garlic have high antioxidants in the form of S-Allylcysteine(SAC) which functions to increase adiponectin.

 Objective: To determine the effect of Black garlic on the histopathological picture of the heart and aorta of obese rats. 

Methods: This study was an experimental study with a randomized post-test only design with control group design with 5 groups of male white rats Sprague Dawley (Rattus novergicus) fed High Fat Fructose. Diet (HFFD) enriched with 1.25% cholesterol and 0.5% cholic acid for 8 weeks and was given black garlic intervention at doses of 450 mg / 200BW, 900mg / 200BW and 1350mg200 / BW for 4 weeks. 

Results: Giving black garlic significantly reduced body weight of rats (p = 0.001), and the results did not significantly reduce heart weight (p = 0.147), aortic weight (p = 0.061), histopathological changes in heart wall thickness (p = 0.423) and aortic wall thickness (p = 0.802). The effective doses of black garlic in this study were 450 mg / 200 grams BW, 900 mg / 200 grams rat BW and 1350 mg / 200 grams BW of rats. The optimal dose is 900 mg / 200 grams BW. 

Conclusion: Black garlic gave a significant reduction in body weight of rats and no significant reduction in heart weight, aortic weight, cardiac and aortic histopathological features. 

Fulltext View|Download
Keywords: Black garlic,;Obesity; Heart and Aortic histopathology

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
Effect of Probiotic Supplementation on Sprague Dawley Rat Liver Histopathology Fed by High Fat High Fructose Diet The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats The Association between Asphyxia and Interleukin (IL)-6 and IL-1β Levels in Neonates Delayed Puberty in Girls with Primary Amenorrhea: A Report of Cases More recent articles
  1. Riset Kesehatan Dasar. Laporan Provinsi Jawa Tengah Riskesdas 2018. Kementerian Kesehatan RI , Hal 119-120. 2018. 119 p
  2. Kementrian Kesehatan Republik Indonesia. Panduan Pelaksanaan Gerakan Nusantara Tekan Angka Obesitas. 2017. p. 32
  3. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González Á, Esquivel-Chirino C, et al. Inflammation, oxidative stress, and obesity. Int J Mol Sci. 2011;12(5):3117–32
  4. Barbosa JAA, Rodrigues AB, Mota CCC, Barbosa MM, Silva ACSE. Cardiovascular dysfunction in obesity and new diagnostic imaging techniques: The role of noninvasive image methods. Vasc Health Risk Manag. 2011;7(1):287–95
  5. Lovren F, Teoh H, Verma S. Obesity and Atherosclerosis: Mechanistic Insights. Can J Cardiol. 2015;31(2):177–83
  6. Haberka M, Skilton M, Biedroń M, Szóstak-Janiak K, Partyka M, Matla M, et al. Obesity, visceral adiposity and carotid atherosclerosis. J Diabetes Complications. 2019;33(4):302–6
  7. Koliaki C, Liatis S, Kokkinos A. Obesity and cardiovascular disease: revisiting an old relationship. Metabolism. 2019;92:98–107
  8. Kang OJ. Physicochemical characteristics of black garlic after different thermal processing steps. Prev Nutr Food Sci. 2016;21(4):348–54
  9. García-Villalón AL, Amor S, Monge L, Fernández N, Prodanov M, Muñoz M, et al. In vitro studies of an aged black garlic extract enriched in S-allylcysteine and polyphenols with cardioprotective effects. J Funct Foods . 2016;27:189–200
  10. Kimura S, Tung YC, Pan MH, Su NW, Lai YJ, Cheng KC. Black garlic: A critical review of its production, bioactivity, and application. J Food Drug Anal. 2017;25(1):62–70
  11. Chang W-T, Shiau D-K, Cheng M-C, Tseng C-Y, Chen C-S, Wu M-F, et al. Black Garlic Ameliorates Obesity Induced by a High-fat Diet in Rats. J Food Nutr Res. 2017;5(10):736–41
  12. Amor S, González-Hedström D, Martín-Carro B, Inarejos-García AM, Almodóvar P, Prodanov M, et al. Beneficial effects of an aged black garlic extract in the metabolic and vascular alterations induced by a high fat/sucrose diet in male rats. Nutrients. 2019;11(1)
  13. Ha AW, Kim WK. Antioxidant mechanism of black garlic extract involving nuclear factor erythroid 2-like factor 2 pathway. Nutr Res Pract . 2017;11(3):206–13
  14. Ma W, Huang T, Zheng Y, Wang M, Bray GA, Sacks FM, et al. Weight-loss diets, adiponectin, and changes in cardiometabolic risk in the 2-year pounds lost trial. J Clin Endocrinol Metab. 2016;101(6):2415–22
  15. Cahyaningrum. Leptin sebagai indikator obesitas, Sandubaya Mataram. J Kesehat Prima. 2015;I(1):1364–71
  16. Carroll JF, Zenebe WJ, Strange TB. Cardiovascular function in a rat model of diet-induced obesity. Hypertension. 2006;48(1):65–72
  17. Mellor K, Ritchie RH, Meredith G, Woodman OL, Morris MJ, Delbridge LMD. High-fructose diet elevates myocardial superoxide generation in mice in the absence of cardiac hypertrophy. Nutrition. 2010;26(7–8):842–8
  18. Aprahamian TR, Sam F. Adiponectin in Cardiovascular Inflammation and Obesity. Int J Inflam. 2011;2011:1–8
  19. Takimoto E, Kass DA. Role of oxidative stress in cardiac hypertrophy and remodeling. Hypertension. 2007;49(2):241–8
  20. Yoo SY, Ahn H, Park YK. High dietary fructose intake on cardiovascular disease related parameters in growing rats. Nutrients. 2017;9(1)

Last update:

No citation recorded.

Last update:

No citation recorded.