DOI: https://doi.org/10.14710/jbtr.v7i2.11524

Antioxidant Total and HOMA-IR of Diabetic Rats Given Crocatum piper and Andrographis paniculata Leaf Extracts

Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 23 Jun 2021; Revised: 4 Aug 2021; Accepted: 9 Aug 2021; Available online: 31 Aug 2021; Published: 31 Aug 2021.
Open Access Copyright (c) 2021 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Background: Type-2 diabetes mellitus (T2DM) is a silent killer which the prevalence continues to increase in every year. Increased oxidative stress occurs in T2DM. Red betel and bitter herb leaf extracts (RBBH) contain a lot of antioxidants. This combination is expected to provide better safety than if used singly because the content of andrographolid in bitter herb has effect such as nausea, vomiting, loss of appetite, and antifertility if consumed in high doses.

Objective: The study aimed to prove the effect of red betel and bitter herb leaf extracts on antioxidant total and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) in T2DM rats given high-fat diet and Streptozotocin (STZ) induction.

Methods: Experimental randomized study with pre-post-test control group design using 25 Sprague Dawley male rats. T2DM model was conducted by providing high-fat feed for 14 days and induction of Streptozotocin-Nicotinamide, then given a combination of red betel and bitter herb leaf extracts at doses of 237.5 mg/kg BW, 225 mg/kg BW, and 212.5 mg/kg BW for 21 days. The measurement of antioxidant total used 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) method. HOMA-IR measured by ELISA (Enzyme-linked immunosorbent assay) method using insulin level equation and fasting blood glucose level measured by glucose oxidase-peroxidase aminoantrypirin (GOD-PAP). Data analysis used paired t-test, wilcoxon test, and ANOVA test to analyze differences in antioxidant total and HOMA-IR value among groups and followed by Bonferroni post-hoc test.

Results: All treatments could reduce HOMA-IR and significantly increase antioxidant total (p<0.05). The most decrease in HOMA-IR and increase in antioxidant total at dose 237.5 mg/kg BW of red betel and bitter herb leaf extracts.

Conclusion: The combination of red betel and bitter herb leaf extracts with dose 237.5 mg/kg BW, 225 mg/kg BW, and 212.5 mg/kg BW can improve blood glucose, insulin, and HOMA-IR levels in type-2 diabetes mellitus rats.

 

Fulltext View|Download
Keywords: T2DM; red betel leaf extract; bitter herb leaf extract; HOMA-IR; antioxidant total

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
The Correlations between Cord Blood Leptin and Leptin Level at Six Months with Infant Growth The Association between Asphyxia and Interleukin (IL)-6 and IL-1β Levels in Neonates Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study Delayed Puberty in Girls with Primary Amenorrhea: A Report of Cases More recent articles
  1. Ayellet, VS., Groop, L., Mootha, VK., Daly, MJ., Altshuler, D. (2010). Common Inherited Variation In Mitochondrial Genes Is Not Enriched For Associations With Type 2 Diabetes Or Related Glycemic Traits. PLoS Genet, 6(8): 1-2
  2. International Diabetes Federation. (2017). Diabetes Atlas 8th Edition. Belgium: International Diabetes Federation
  3. Huang, PL. (2009). A Comprehensive Definition For Metabolic Syndrome. Dis Model Mech, 2(5–6):231–237
  4. Esteghamati, A., Ashraf, H., Khalilzadeh, O., Zandieh A., Nakhjavani, M., Rashidi, A. (2010). Optimal Cut-off of Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) for The Diagnosis of Metabolic Syndrome: Third National Surveillance of Risk Factors of Non- Communicable Diseases in Iran (SuRFNCD-2007). Nutr Metab, 7(1):1–8
  5. Jawi, IM., Sumardika, IW., Linawati, NM. (2014). Pencegahan Gangguan Fungsi Ginjal Karena Stres Oksidatif pada Tikus Diabetes dengan Ubi Jalar Ungu. J Vet, 15(2): 274–280
  6. Mohieldein, AH., Hasan, M., Al-Harbi, KK., Alodailah, SS., Azahrani, RM., Al-Mushawwah, SA. (2015). Dyslipidemia And Reduced Total Antioxidant Status In Young Adult Saudis With Prediabetes. Diabetes Metab Syndr Clin Res, 9(4): 287–291
  7. Lee, SG., Wang, T., Vance, T., Hurbert, P., Koo, SI., Chun, OK. (2015). Evaluation Of Analytical Methods For Measuring Plasma Antioxidant Capacity by Comparing With Urinary 8-Isoprostane Concentration. Faseb J, 29(1): 922-928
  8. Prameswari, OM., Widjanarko, SB. (2014). The Effect of Water Extract of Pandan Wangi Leaf to Decrease Blood Glucose Levels and Pancreas Histopathology at Diabetes Mellitus Rats. J Vet, 2(2): 16–27
  9. Siregar, AA., Harahap, U., Mardianto. (2015). Ekstrak Etanol Daun Sirih Merah (Piper crocatum) Menurunkan Kadar Gula Darah Mencit Diabetes. J Ilm Manuntung, 1(4): 42–46
  10. Thakur, AK., Chatterjee, SS., Kumar, V. (2014). Antidiabetic Activity of Standardized Extract of Andrographis paniculata in Rodents. J Pharma Nutr, 2(3):91–92
  11. Yusuf, AL., Adeyemi, KD., Roselina, K., Alimon, AR., Goh, YM., Samsudin, AA. (2018). Dietary Supplementation of Different Parts of Andrographis paniculata Affects The Fatty Acids, Lipid Oxidation, Microbiota, And Quality Attributes of Longissimus Muscle In Goats. Food Res Int, 111: 699–707
  12. Direktorat Obat Asli Indonesia. Sediaan Antikanker Andrographidis Paniculatae Herbae (Herba Sambiloto) Jenis: Andrographis paniculata (Burm. f.) Nees. (2010). Acuan Sediaan Herbal BPOM RI, 5: 112–132
  13. Yulinta, NMR., Gelgel, KTP., Kardena, IM. (2013). Efek Toksisitas Ekstrak Daun Sirih Merah Terhadap Gambaran Mikroskopis Ginjal Tikus Putih Diabetik yang Diinduksi Aloksan. Bul Vet Udayana, 5(2): 91–102
  14. Mulqie, L., Hazar, S., Septian, AF. (2013). Aktivitas Antibakteri Fraksi Etil Asetat Daun Sambiloto (Andrographis paniculata (Burm.f) Wallich ex Nees) Terhadap Bakteri Bacillus subtilis ATCC 6633. J Farm Galen, 4(2): 33–37
  15. World Health Organization. (2000). General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine. WHO, 1: 1–71
  16. Ghasemi, A., Khalifi, S., Jedi, S. (2014). Streptozotocin-Nicotinamide-Induced Rat Model of Type 2 Diabetes. Acta Physiol Hung, 101(4):408–420
  17. Gheibi, S., Kash, K., Ghasemi, A. (2017). A Practical Guide For Induction of Type-2 Diabetes in Rat: Incorporating a High-Fat Diet and Streptozotocin. Biochem Pharmacol, 95(11): 605–613
  18. Skovso, S. (2014). Modeling Type 2 Diabetes In Rats Using High Fat Diet and Streptozotocin. J Diabetes Investig, 5(4): 349–358
  19. Zhou, SS., Li, D., Sun, WP., Guo, M., Lun, YZ., Zhou, YM. (2009). Nicotinamide Overload May Play a Role In The Development of Type 2 Diabetes. World J Gastroenterol, 15(45): 5674–5684
  20. Made, L. (2014). Efektifitas Ekstrak Daun Sirih Merah (Piper crocatum) Terhadap Peningkatan Berat Badan Tikus Putih (Rattus novergicus) Jantan Kondisi Diabetes yang di Induksi Aloksan. Bul Vet Udayana, 6(2): 175–179
  21. Saputra, A., Andayani, S., Nursyam, H. (2016). Total Quantity of Phenol and Isolation Methanol Tannin Extract of Red Betel Leaf (Piper crocatum). Int J PharmaTech Res, 9(7): 146–153
  22. Adedapo, AA., Adeoye, BO., Sofidiya, MO., Oyagbemi, AA. (2015). Antioxidant, antinociceptive and anti-inflammatory properties of the aqueous and ethanolic leaf extracts of Andrographis paniculata in some laboratory animals. J Basic Clin Physiol Pharmacol, 26(4): 327–334
  23. Afsari, R., Kusmiyati, Merta, IW. (2016). Pengaruh Pemberian Ekstrak Daun Sirih Merah (Piper Crocatum) Terhadap Penurunan Kadar Gula Darah Mencit (Mus musculus). Biol Trop, 16(1): 49–55
  24. Pradini, SA., Pambudi, PR., Dinah, FA. (2017). Uji Efek Antidiabetik Kombinasi Ekstrak Etanol Daun Stevia (Stevia Rebaudiana Bert.) Dan Daun Sambiloto (Andrographis folium) Pada Tikus Jantan Galur Wistar Yang Diinduksi Aloksan. J Vet, 4(2): 177–182
  25. Royani, JI., Hardianto, D., Wahyuni, S. (2014). Analysis of Andrographolide Contents on Sambiloto Plants (Andrographis paniculata) Derived from 12 Locations in Java Island. Bioteknol Biosains Indones, 1(1): 15–20
  26. Hasibuan, MS., Yasni, S., Bintang, M., Ranti, AS. (2016). Antihyperglycemic Activity of Piper crocatum Leaves and Cinnamomum burmannii Bark Mixture Extract in Streptozotocin-induced Diabetic Rats. J Math Fundam Sci, 48(2): 178–191
  27. Arjadi, F., Mustofa. (2017). Ektrak Daging Buah Mahkota Dewa Meregenerasi Sel Pulau Langerhans Pada Tikus Putih Diabetes. J iImiah Biol, 5(1): 27–33
  28. Larantukan, SVM., Setiasih, NLE., Widyastuti, SK. (2014). Pemberian Ekstrak Etanol Kulit Batang Kelor Glukosa Darah Tikus Hiperglikemia. Indones Med Veterinus, 3(4): 292–299
  29. Pitriya, IA., Rahman, N., Sabang, SM. (2017). Efek Ekstrak Buah Kelor (Moringa oleifera) Terhadap Penurunan Kadar Gula Darah Mencit (Mus musculus). J Akad Kim, 6(1): 35–42
  30. Qatanani, MA., Lazar, M. (2010). Mechanisms Of Obesity-Associated Insulin Resistance: Many Choice On The Menu. Genes Dev, 36(215): 490–499
  31. Akbar, S. (2011). Andrographis paniculata: A Review of Pharmacological Activities and Clinical Effects. Altern Med Rev, 16(1): 66–77
  32. Basheer, L., Kerem, Z. (2015). Interactions Between CYP3A4 And Dietary Polyphenols. Oxid Med Cell Longev, 15: 1–15
  33. Hou, XL., Qiu, F., Kang, N., Chen, LX., Takahashi, K., Azuma, J. (2011). Andrographolide Inhibits The Expression And Metabolic Activity Of Cytochrome P450 3A4 In The Modified Caco-2 Cells. J Ethnopharmacol, 141(2): 709–713

Last update:

No citation recorded.

Last update:

No citation recorded.