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The Association between Asphyxia and Interleukin (IL)-6 and IL-1β Levels in Neonates

1Faculty of Medicine, Jenderal Soedirman University, Indonesia

2Faculty of Medicine, Universitas Diponegoro, Indonesia

Received: 6 Jun 2021; Revised: 5 Aug 2021; Accepted: 12 Aug 2021; Available online: 31 Aug 2021; Published: 31 Aug 2021.
Open Access Copyright (c) 2021 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Neonatal asphyxia is a respiratory failure during and just after birth. It can cause morbidity and mortality in neonates.Interleukin (IL)-6 and IL-1β are inflammatory cytokines produced by neuronal cells in early response to brain injury due to asphyxia. However, their role in neonatal asphyxiais remain elusive.

Objective: To determine theassociation between asphyxia and serumIL-6 and IL-1β levelsin neonates.

Methods: Across-sectional study was conducted on neonates diagnosed with moderate to severe asphyxia who hospitalized atthe Dr. Kariadi General Hospital Semarang Indonesia from December 2013 to May 2014. The subjects were examined for serum IL-6 and IL-1β. Blood samples were obtained from umbilical vein in the first 24 hours of life. Serum IL-6 and IL-1β levelswere measured using immunoassay. Dependent variable were IL-6 and IL-1β level. Bivariate analysis was performed using chi-square test, for the assessment of the association between dependent and independent variables. A p-value of less than 0.05 was considered statistically significant.

Result: A total of 54 subjectswere enrolled in this study. No significant difference between moderate and severe asphyxia neonatesin term ofsex, birthweight,type of delivery, neonate’s mother age, gestational age, and parity. Levels of IL-6 and IL-1β levels wereincreased significantly in both moderate and severe asphyxiagroups, and the levels were significant higher in the severe asphyxia than that of in the moderate, p=0.003 and p=0.007, respectively.

Conclusion:There was association between asphyxia and IL-6 and IL-1β levelsin neonates.IL-6 and IL-1β levelswere increased in neonates with moderate and severe asphyxia, with extend of increase was significant higherin the later.

 

  

 

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Keywords: Neonatal asphyxia; IL-6; and IL-1β

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  1. Utomo MT. Risk Factors for Birth Asphyxia. Folia Medica Indonesiana. 2011;47(4):211-4
  2. Antonucci R, Porcella A, Pilloni M. Perinatal asphyxia in the term newborn. Journal of Pediatric and Neonatal Individualized Medicine. 2014;3(2):1-14
  3. Morales P, Bustamante D, Espina-Marchant P, Neira-Pena T, Gutierrez-Hernandez MA, Allende-Castro C, et al. Pathophysiology of perinatal asphyxia: can we predict and improve individual outcomes? EPMA J. 2011;2(2):211-30
  4. Ambalavanan N, Carlo W. Hypoxia-ischemia. In: Behrman R, Kliegman R, Jenson H, editors. Nelson Textbook of Pediatrics. Philadelphia: Saunders; 2011. p. 569-73
  5. Abdo RA, Halil HM, Kebede BA, Anshebo AA, Gejo NG. Prevalence and contributing factors of birth asphyxia among the neonates delivered at Nigist Eleni Mohammed memorial teaching hospital, Southern Ethiopia: a cross-sectional study. BMC Pregnancy Childbirth. 2019;19(1):536
  6. Polglase GR, Ong T, Hillman NH. Cardiovascular Alterations and Multiorgan Dysfunction After Birth Asphyxia. Clin Perinatol. 2016;43(3):469-83
  7. Chalak LF, Laptook AR, Perlman JM, Garcia D, Ramilo O, Wyckoff MH. Immediate pro-inflammatory cytokine response associated with asphyxia and volume infusions in a neonatal piglet model. Journal of Neonatal-Perinatal Medicine. 2008;1:69-75
  8. Li B, Concepcion K, Meng X, Zhang L. Brain-immune interactions in perinatal hypoxic-ischemic brain injury. Prog Neurobiol. 2017;159:50-68
  9. Erta M, Quintana A, Hidalgo J. Interleukin-6, a major cytokine in the central nervous system. Int J Biol Sci. 2012;8(9):1254-66
  10. Szpecht D, Wiak K, Braszak A, Szymankiewicz M, Gadzinowski J. Role of selected cytokines in the etiopathogenesis of intraventricular hemorrhage in preterm newborns. Childs Nerv Syst. 2016;32(11):2097-103
  11. Li S-j, Liu W, Wang J-l, Zhang Y, Zhao D-j, Wang T-j, et al. The role of TNF-α, IL-6, IL-10, and GDNF in neuronal apoptosis in neonatal rat with hypoxic-ischemic encephalopathy. European Review for Medical and Pharmacological Sciences. 2014;18(6):905-9
  12. Puhl SL, Steffens S. Neutrophils in Post-myocardial Infarction Inflammation: Damage vs. Resolution? Front Cardiovasc Med. 2019;6:25
  13. Petković F, Castellano B. The role of interleukin-6 in central nervous system demyelination. Neural Regen Res. 2016;11(12):1922-3
  14. Jung JE, Kim GS, Chan PH. Neuroprotection by interleukin-6 is mediated by signal transducer and activator of transcription 3 and antioxidative signaling in ischemic stroke. Stroke. 2011;42(12):3574-9
  15. Okazaki K, Kuboi T, Kusaka T, Kondo M, Kimura H. Pathophysiological Roles of Cytokines in the Brain During Perinatal Asphyxia. Ann Pediatr Child Health. 2015;3(1):1-10
  16. Helsinki WMADo. Recommendations guiding physicians in biomedical research involving human subjects 1996 [Available from: https://www.wma.net/wp-content/uploads/2016/11/DoH-Oct1996.pdf
  17. Chiesa C, Pellegrini G, Panero A, De Luca T, Assumma M, Signore F, et al. Umbilical cord interleukin-6 levels are elevated in term neonates with perinatal asphyxia. Eur J Clin Invest. 2003;33(4):352-8
  18. Bharathi B, Bhat BV, Negi VS, Adhisivam B. Inflammatory mediators as predictors of outcome in perinatal asphyxia. Indian J Pediatr. 2015;82(5):433-8
  19. Bhalala US, Koehler RC, Kannan S. Neuroinflammation and neuroimmune dysregulation after acute hypoxic-ischemic injury of developing brain. Front Pediatr. 2014;2:144
  20. McAdams RM, Juul SE. The Role of Cytokines and Inflammatory Cells in Perinatal Brain Injury. Neurology Research International. 2012;2012:561494
  21. Naithani M, Simalti A. Biochemical Markers in Perinatal Asphyxia. Journal of Nepal Paediatric Society. 2011;31(2):151-6
  22. Ahearne CE, Chang RY, Walsh BH, Boylan GB, Murray DM. Cord Blood IL-16 Is Associated with 3-Year Neurodevelopmental Outcomes in Perinatal Asphyxia and Hypoxic-Ischaemic Encephalopathy. Dev Neurosci. 2017;39(1-4):59-65
  23. Ramaswamy V, Horton J, Vandermeer B, Buscemi N, Miller S, Yager J. Systematic review of biomarkers of brain injury in term neonatal encephalopathy. Pediatr Neurol. 2009;40(3):215-26
  24. Nu ̃nez A, Benavente I, Blanco D, Boix HCn, F., Chaffanel M, Fernández-Colomerg B, et al. Oxidative stress in perinatal asphyxia and hypoxic-ischaemic encephalopathy. An Pediatr (Barc). 2018;88:228.e1-.e9
  25. Koehler RC, Yang ZJ, Lee JK, Martin LJ. Perinatal hypoxic-ischemic brain injury in large animal models: Relevance to human neonatal encephalopathy. J Cereb Blood Flow Metab. 2018;38(12):2092-111
  26. Jacobson Misbe EN, Richards TL, McPherson RJ, Burbacher TM, Juul SE. Perinatal asphyxia in a nonhuman primate model. Dev Neurosci. 2011;33(3-4):210-21
  27. Boskabadi H, Afshari J, Ghayour-Mobarhan M, Maamouri G, Shakeri M, Sahebkar A, et al. Association between serum interleukin-6 levels and severity of perinatal asphyxia. Asian Biomedicine. 2010;4(1):79-85
  28. Boskabadi H, Moradi A, Zakerihamidi M. Interleukins in diagnosis of perinatal asphyxia: A systematic review. Int J Reprod Biomed. 2019;17(5)
  29. Paliwal P, Varma M, Mulye S, Paliwal M, Shaikh M. Study of Neurological Marker in Perinatal Asphyxia and Its Correlation with Different Stages of Hypoxic Ischemic Encephalopathy. International Journal of Scientifi c Study. 2014;2(7):40-3
  30. Procianoy RS, Silveira RC. The role of sample collection timing on interleukin-6 levels in early-onset neonatal sepsis. J Pediatr (Rio J). 2004;80(5):407-10
  31. Wagdy E-S, Hasan E, Eman E. Interleukin-1β in perinatal asphyxia. Benha Medical Journal. 2016;33(2)
  32. Bajnok A, Berta L, Orbán C, Veres G, Zádori D, Barta H, et al. Distinct cytokine patterns may regulate the severity of neonatal asphyxia-an observational study. J Neuroinflammation. 2017;14(1):244-
  33. Varsami M, Xanthos T, Aroni F, Argyri I, Lelovas P, Dontas I, et al. Inflammation and oxidative stress biomarkers in neonatal brain hypoxia and prediction of adverse neurological outcome: a review. Journal of Pediatric and Neonatal Individualized Medicine. 2013;2(2):1-14
  34. Neira-Pena T, Rojas-Mancilla E, Munoz-Vio V, Perez R, Gutierrez-Hernandez M, Bustamante D, et al. Perinatal asphyxia leads to PARP-1 overactivity, p65 translocation, IL-1beta and TNF-alpha overexpression, and apoptotic-like cell death in mesencephalon of neonatal rats: prevention by systemic neonatal nicotinamide administration. Neurotox Res. 2015;27(4):453-65
  35. Aly H, Khashaba MT, El-Ayouty M, El-Sayed O, Hasanein BM. IL-1beta, IL-6 and TNF-alpha and outcomes of neonatal hypoxic ischemic encephalopathy. Brain Dev. 2006;28(3):178-82

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