DOI: https://doi.org/10.14710/jbtr.v7i1.10129

Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies

Universiti Kebangsaan Malaysia, Malaysia

Received: 20 Jan 2021; Revised: 12 Mar 2021; Accepted: 13 Apr 2021; Available online: 30 Apr 2021; Published: 30 Apr 2021.
Open Access Copyright (c) 2021 Journal of Biomedicine and Translational Research

Citation Format:
Abstract

Colon cancer-associated gut bacteria were mostly identified via next-generation sequencing in gut microbiome profiling studies. Anaerobic culture systems can be used to culture colon cells with these gut bacteria to further confirm the tumorigenic properties of these bacteria. Nevertheless, it is unclear how colon cells will grow in an anaerobic environment, as most cells are cultured aerobically. Therefore, we investigated the survival and viability of HT-29, a colon cancer cell line in an anaerobic culture system, and compared it to the usual culture condition in an aerobic setup. Interestingly, we found that HT-29 was able to grow in the anaerobic setup. Its viability was similar for both culture conditions, with only a slower growth rate observed in the anaerobic setup. Furthermore, gene expression studies showed that the cells were not under severe anaerobic stress even when exposed to the oxygen-deprived environment.This study provided results on some baseline parameters of an anaerobic colon cell culture system, and will be useful for journal readers who wish to investigate functional properties of anaerobic bacteria.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Instrument
Cover Letter
Subject
Type Research Instrument
  Download (13KB)    Indexing metadata
 Research Instrument
Conflict of Interest and CTA
Subject
Type Research Instrument
  Download (540KB)    Indexing metadata
Keywords: anaerobic culture system; cell viability; colorectal cancer; HT-29; hypoxic markers
Funding: Ministry of Higher Education Malaysia under contract FRGS/1/2018/SKK11/UKM/02/2

Article Metrics:

Article Info
Section: Letters to the Editor
Language : EN
Recent articles
The Evaluation of Parental Acceptance Towards Children with Sex Chromosomal Disorders of Sex Development Using A Mixed-Method Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats B- Acute Lymphoblastic Leukemia L2 In Second Trimester of Pregnancy Degree of coronary occlusions links to the patient clinical outcome: four cases of double culprits acute ST-segment elevation myocardial infarction Fragmentomic cfDNA Patterns in Noninvasive Prenatal Testing and Beyond Prerequisite Evaluation of Anaerobic Settings for Gut Microbiome Functional Studies More recent articles
  1. Jahani-Sherafat S, Alebouyeh M, Moghim S, Amoli HA, Ghasemian-Safaei H. Role of gut microbiota in the pathogenesis of colorectal cancer; a review article. Gastroenterol. Hepatol. Bed Bench. 2018; 11(2):101–109
  2. Martínez-Maqueda D, Miralles B, Recio, I. HT29 cell line. In: Verhoeckx K, Cotter P, Kleiveland C, Mackie A, Swiatecka D, Lópex-Expósito I, et al., editors. The impacts of food bioactives on health. Cham: Springer; 2015, pp. 113–124
  3. Delaney ML, Onderdonk AB. Evaluation of the AnaeroPack system for growth of clinically significant anaerobes. J. Clin. Microbiol. 1997; 35(3):558–562
  4. Kalyanaraman B. Teaching the basics of cancer metabolism: developing antitumor strategies by exploiting the differences between normal and cancer cell metabolism. Redox Biol. 2017; 12:833–842
  5. Eales KL, Hollinshead KER, Tennant DA. Hypoxia and metabolic adaptation of cancer cells. Oncogenesis. 2016; 5(1): e190

Last update:

No citation recorded.

Last update:

No citation recorded.