skip to main content

IDENTIFICATION OF RAT MEATBALLS IN TRADITIONAL MARKET IN AREA OF JAKARTA USING REAL TIME–PCR

*Triayu Septiani  -  Halal Research Center, YARSI Research Institute, Jakarta, Indonesia
Open Access Copyright (c) 2021 Indonesia Journal of Halal under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Abstract

Meat is one of protein source who was often consumed. Consume halal food are obligation for every moslem. Beef and chicken meat is the most popular food consumed in Indonesia, processed beef and chicken meat is the most consumed, example meatballs. Usually processed meat food from beef are often counterfeiting with rat meat. Rat is generally become pests in Indonesia, such in rice fields and rice storage warehouses, Rat has orange-brown fur and has sized between 304-400 mm. Rattus rattus or black rats are mice that can be found all over the world. The analysis technique in biology molecular, such RT – PCR becomes a promises technique for detection or identification contaminant DNA in meat processed food. All of the samples has been result of extraction then analyzed the purity and has purity range between 1.8 to 1.89. Results of Real Time PCR to detection rat DNA contaminants in meatball samples showed that all of the samples (forty-six samples) who was analyzed, five samples showed negative detected samples and forty-one other samples has Cp value range between 27.02 to 37.08, were indicated to be positively indicated from amplified samples, but the Cp value indicated in the samples was indicated positive tend to be much higher than the positive control Cp value, which is 16,26.

Fulltext View|Download
Keywords: DNA, Meatballs, Rat, RT-PCR

Article Metrics:

  1. Ali, M.E., hashim, U., Mustafa, S., Man, Y.B.C., Dhahi, Th.S., Kashif, M., Uddin, M.K., Hamid, S.B.A. 2012. Analysis of Pork Adulteration in Commercial Meatballs Targeting Porcine – Specific Mitochondrial Cytochrome B Gene by Taqman Probe Real – Time Polymerase Chain Reaction. Journal of Meat Science 91 (2012) 454 – 459
  2. Aziz, A.Y. and Vui, C.N. 2012. The Role of Halal Awareness and Halal Certification in Influencing Non-Muslim’s Purchase Intention. University Putra Malaysia. Third International Conference on Business and Economic Research (3rd ICBER 2012) Proceeding, ISBN: 978-967-5705-05-2
  3. Bohari, A. M., Hin, C. W. and Fuad, N. 2013. The Competitiveness of Halal Food Industry in Malaysia : A SWOT ICT Analysis. Geografia Online Malaysia Journal of Society and Space 9 issue 1 (1 - 9) 1, ISSN 2180-2491
  4. Heinz, G., Hautzinger, P. 2007. Meat Processing Technology For Small To Medium Scale Producers. FAO Regional Office For Asia and The Pacific, RAP Publication 2007 / 20
  5. Farag, M.R., Alagawany, M., El-Hack, M. E. A., Tiwari, R., Dhama, K. 2015. Identification of Different Animal Species in Meat and Meat Products: Trends and Advances. Jour. of Adv. Anim. Vet. Sci. 3(6): 334 – 346
  6. Ilhak, O.I., Arslan, A. 2007. Identification of Meat Species by Polymerase Chain Reaction (PCR) Technique. Turk. J. Vet. Anim. Sci: 31(3): 159 – 163
  7. Janosi, A. 2006. Species Specific Detection of Meat by Polymerase Chain Reaction Techniques. Corvinus University of Budapest, Budapest, Rumania
  8. Joshi, M., Deshpande, J.D. 2010. Polymerase Chain Reaction: Methods, Principles and Application. International Journal of Biomedical Research 1 (5) 81 – 97
  9. Jonker, K.M., Tilburg, J.J.H.C., Hagele, G.H., Boer, E.D. 2008. Species Identification in Meat Products Using Real – Time PCR. Food Aditives and Contaminants: Part A, 25:5, 527 – 533
  10. Mursyidi, A. 2012. The Role Of Chemical Analysis in the Halal Authentication of Food and Pharmaceutical Products. J. Food Phar.Sci. 1 (2013) 1 – 4
  11. Murugaiah, C., Noor, Z.M., Mastakim, M., Bilung, L.M., Selamat, J., Radu, S. 2009. Meat Species Identification and Halal Authentication Analysis Using mitochondrial DNA. Elsevier Journal of Meat Science 83 (2009) 57 – 61
  12. Raharjo, T.J., Pratama, G.A., Nuryanti, I., Pratiwi, R. 2019. Forgery Detection Beef With Mice Meat (Mus muculus in Meatballs Using Real – Time Polymerase Chain Reaction (Real – Time PCR) Primer Specific for a Target Mitochondrial DNA ND-1Gene. Indones. J. Chem., 2019 (1), 89 – 96
  13. Sahilah, A.M., Norhayati, Y., Norrakiah, A. S., Aminah, A. and Wan Aida, W. M. 2011. Halal Authentication of Raw Meats Using PCR Amplification of Mitochondrial DNA. International Food Research Journal 18(4): 1489-1491 (2011)
  14. Salehudin, I. 2010. Halal Literacy: A Concept Exploration and Measurement Validation. Dept. of Management, Faculty of Economics, University of Indonesia. Asean Marketing Journal, June 2010 – Vol. II – No. 1
  15. Unajak, S., Meesawat, P., Anyamaneeratch, K. Anuwareepong, D., Srikulnath, K., Choowongkomon, K. 2011. Identification of Species (Meat and Blood Samples) Using Nested-PCR Analysis of Mitochondrial DNA. African Journal of Biotechnology Vol. 10(29), pp. 5670-5676, 22 June, 2011
  16. Widyasari, Y. I., Sudjadi., Rohman, A. 2015. Detection of Rat Meat Adulteration in Meat Ball Formulations Employing Real Time PCR. Asian Journal of Animal Sciences 9 (6): 460 – 465, 2015
  17. Zidek, R., Bajzik, P., Marsalkova, L., Golian, J. Detection of Counterfeit Beef Using Real – Time PCR. 2011. Dept. of Hygiene and Food Safety, Slovak University of Agriculture in Nitra

Last update:

No citation recorded.

Last update:

No citation recorded.