DOI: https://doi.org/10.14710/dimj.v3i1.14955

Wound Healing Response to Full-Thickness Skin Defect Sprague Dawley Rats Given Ozonated Black Cumin Oil Viewing from The Profile of Neutrophil and Macrophage Count

*Igor Rizkia Syahputra  -  Departement of Biomedic, Faculty of Medicine,Universitas Diponegoro,Jl. Prof Sudarto,SH,Tembalang,Semarang, Indonesia 50275, Indonesia
Open Access Copyright (c) 2022 Diponegoro International Medical Journal
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Abstract
The incidence of wounds keeps increasing and inadequate treatment can cause the wound to become infected. Ozone has antibacterial properties and can help activate pro-inflammatory agents in the wound healing process; one of the methods is oil ozonation, for example with black cumin oil. Topical administration of black cumin plays a role in wound healing because of its antibacterial and antifungal properties, and plays a role in increasing the epitalization process. To determine the effectiveness of applying ozonated black cumin oil (Nigella sativa) on the healing process of full-thickness skin defects in Sprague Dawley rats, in terms of the number of macrophage and neutrophil cells. This research was an experimental study with a post-test only control group design method. Total sample of 48 Sprague Dawley rats was divided into 6 groups. The first group was given gentamicin ointment, the second group was given 0.9% normal saline, the third to the fifth group was given ozone black with doses of ozone respectively, namely 1400 mg/ml, 1800 mg/ml and 2200 mg/ml. Incision for taking the tissue samples was held on the 3rd and 7th day according to the treatment group. The tissue samples were subjected to histopathological examination with HE staining. A significant decrease in neutrophil levels occurred at the dose of 1,400mg/ml ozonated black cumin oil on day 7 (p=<0.001), dose of 1,800 mg/ml on day 3 (p=0.021) and day 7 (p=<0.001), doses of 2,200mg/ml at day 3 (p=0.020) and day 7 (p=<0.001) compared with a positive control group (given gentamicin). A significant decrease in macrophage levels occurred at a dose of 1,400mg/ml on day 3 (p=0.037) and 7 (p=0.019), a dose of 1,800 mg/ml on day 3 (p <0.001) and 7 (p=0.020), dose 2,200mg/ml on day 3 (p=<0.001) and day 7 (p=0.020).

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Respon Penyembuhan Luka pada Full-Thickness Skin Defect Tikus Sprague Dawley yang Diberikan Minyak Jintan Hitam Terozonisasi Dilihat dari Profil Jumlah Neutrofil dan Jumlah Makrofag
Subject Full-thickness skin defect, minyak jintan terozonisasi, neutrofil
Type Research Results
  Download (7MB)    Indexing metadata
Keywords: Full-thickness skin defect,macrophage, ozonated cumin oil,neutrophile
Funding: Universitas Diponegoro under contract MetMu123456

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Section: Original Research Article
Language : EN
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