skip to main content

Peningkatan Pertumbuhan Tajuk dan Nilai Estetika Tanaman Hias Epipremnum aureum pada Aplikasi Sitokinin Sintetik (6-benzylaminopurine)

Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro,Jl. Prof. Jacob Rais Tembalang, Semarang, 50275, Indonesia

Open Access Copyright 2025 Buletin Anatomi dan Fisiologi

Citation Format:
Abstract

Kualitas tanaman hias daun ditentukan oleh pertumbuhan dan nilai estetika tajuk.  Hormon sitokinin berpengaruh terhadap pertumbuhan dan produktivitas tajuk. Penelitian ini mengkaji respon pertumbuhan tajuk, morfologis daun dan nilai estetika tanaman hias Epipremnum aureum terhadap berbagai konsentrasi hormon sitokinin sintetis (6-benzylaminopurine /BAP). Aplikasi BAP dengan konsentrasi 0, 5, 25 dan 125 ppm diberikan melalui metode spray pada daun stek E. aureum umur 3 MST. Pemberian BAP dilakukan satu kali dalam seminggu hingga tanaman berumur 6 MST, selanjutnya diberikan dua hari sekali hingga penelitian berakhir pada 9 MST. Respon tanaman diamati pada umur 9 MST meliputi: respon pertumbuhan (jumlah dan luas daun, panjang batang, berat segar daun, berat segar batang), morfologis daun (warna dan indeks warna daun), dan nilai estetika (corak daun). Hasil penelitian menunjukkan aplikasi BAP 5 ppm, 25 ppm dan 125 ppm meningkatkan pertumbuhan tajuk dibanding kontrol. Peningkatan konsentrasi BAP hingga kisaran 80-100 ppm meningkatkan pertumbuhan tajuk, namun konsentrasi di atas 100 ppm menunjukkan kecenderungan penurunan pertumbuhan. Secara morfologis, peningkatan konsentrasi BAP meningkatkan warna hijau dan indeks warna daun serta pembentukan corak daun, terutama pada konsentrasi BAP 125 ppm. Daun menunjukkan warna hijau yang lebih intens dan corak semburat kekuningan yang lebih tinggi dibandingkan perlakuan lainnya.  Konsentrasi BAP yang optimal untuk meningkatkan pertumbuhan tanaman ditemukan pada kisaran 80-100 ppm. Secara keseluruhan, aplikasi BAP meningkatkan pertumbuhan tajuk dan memperbaiki kualitas estetika daun tanaman E. aureum.

 


The growth and aesthetic value of the shoot determine the quality of ornamental foliage plants. Cytokinin hormones influence the growth and productivity of the shoot. This study examines the response of canopy growth, leaf morphology, and the aesthetic value of the ornamental plant Epipremnum aureum to various concentrations of synthetic cytokinin hormone (6-benzylaminopurine/BAP). BAP application at concentrations of 0, 5, 25, and 125 ppm was applied through a spray method to the leaves of 3-month-old E. aureum cuttings. BAP was used once a week until the plants were 6 months old, then twice every two days until the study ended at 9 months. The plant responses were observed at 9 months of age, including growth responses (number and size of leaves, stem length, fresh leaf weight, and fresh stem weight), leaf morphology (color and leaf color index), and aesthetic value (leaf patterns). The results showed that applying BAP at 5 ppm, 25 ppm, and 125 ppm increased canopy growth compared to the control. Increasing the BAP concentration to 80-100 ppm enhanced canopy growth, but concentrations above 100 ppm tended to decrease growth. Morphologically, increasing BAP concentrations improved the green color and leaf color index and the formation of leaf patterns, especially at the 125 ppm concentration. The leaves showed a more intense green color and higher yellowish patterns than the other treatments. The optimal BAP concentration for improving plant growth was 80-100 ppm. Overall, the BAP application enhanced canopy growth and improved the aesthetic quality of E. aureum leaf plants.

Fulltext View|Download
Keywords: 6-benzyl aminopurine; sitokinin; Epipremnum aureum; pertumbuhan; klorofil
Funding: Universitas Diponegoro under contract 233-108/UN7.6.1/PP/2021

Article Metrics:

  1. Ali, S., Basit, A., Khattak, A. M., Shah, S. T., Ullah, I., Khan, N. A., Ahmad, I., Rauf, K., Khan, S., Ullah, I., & Ahmad, I. (2021). Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching. Pakistan Journal of Agricultural Research, 34(1), 29–40. https://doi.org/10.17582/journal.pjar/2021/34.1.29.40
  2. Ara Noor-E-Ferdous, R., & Sarker, B. C. (2021). Effects of benzyl aminopurine (BAP) on growth, yield and biochemical characteristics of summer mungbean cultivars. In International Journal of Agriculture and Medicinal Plants, 2(1)
  3. Aremu, A. O., Fawole, O. A., Makunga, N. P., Masondo, N. A., Moyo, M., Buthelezi, N. M. D., Amoo, S. O., Spíchal, L., & Doležal, K. (2020). Applications of cytokinins in horticultural fruit crops: Trends and future prospects. Biomolecules, 10(9), 1–71. https://doi.org/10.3390/biom10091222
  4. Basra, S. M., & Lovatt, C. J. (2016). Exogenous applications of moringa leaf extract and cytokinins improve plant growth, yield, and fruit quality of cherry tomato. HortTechnology, 26(3), 327-337
  5. Cackett, L., Luginbuehl, L. H., Schreier, T. B., Lopez-Juez, E., & Hibberd, J. M. (2022). Chloroplast development in green plant tissues: the interplay between light, hormone, and transcriptional regulation. In New Phytologist, 233(5): 2000–2016. https://doi.org/10.1111/nph.17839
  6. Campolongo, L., Carnelos, D., Miglioli, J. L., Fuginuma, P., Giardina, E., & Di Benedetto, A. (2020). Physiological mechanism involved in the response to four lettuce varieties to a pre-transplant root restriction and a 6, benzyl aminopurine (BAP) spray. Asian Journal of Agricultural and Horticultural Research, 5(4), 27-43
  7. Cavusoglu, S., Sensoy, S., Karatas, A., Tekin, O., Islek, F., Yilmaz, N., Kipcak, S., Ercisli, S., Skrovankova, S., Adamkova, A., & Mlcek, J. (2021). Effect of pre-harvest organic cytokinin application on the post-harvest physiology of pepper (Capsicum annuum l.). Sustainability (Switzerland), 13(15). https://doi.org/10.3390/su13158258
  8. Chen, L., Zhao, J., Song, J., & Jameson, P. E. (2021). Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement. In Plant Biotechnology Journal, 19(5), 878–896. https://doi.org/10.1111/pbi.13595
  9. Chen, Y. S., Chesson, P., Wu, H. W., Pao, S. H., Liu, J. W., Chien, L. F., Yong, J. W. H., & Sheue, C. R. (2017). Leaf structure affects a plant’s appearance: combined multiple-mechanisms intensify remarkable foliar variegation. Journal of Plant Research, 130(2), 311–325. https://doi.org/10.1007/s10265-016-0890-4
  10. Danish, S., Rehman, R. A., Maqbool, A., Ali, M., Idrees, M., Irshad, I., Munir, S., Alahmadi, T. A., & Ansari, M. J. (2024). Effects of Benzyl Amino Purine on Growth, Antioxidants, and Chloropyll Contens of Phaseolus vulgaris L. Cultivated Under Heat Stress. Pakistan Journal of Botany, 56(5), 1687–1696. https://doi.org/10.30848/pjb2024-5(29)
  11. Du, F., Guan, C., & Jiao, Y. (2018). Molecular Mechanisms of Leaf Morphogenesis. In Molecular Plant, 11(9), 1117–1134. https://doi.org/10.1016/j.molp.2018.06.006
  12. Dussan-Currea, S. L., Miranda-Lasprilla, D., & Balaguera-López, H. E. (2025). Effect of 6-BAP application on leaf yellowing of Chrysanthemum morifolium Ram cv.'Shrek'and'Bomber Green'. Revista Colombiana de Ciencias Hortícolas, 19(1), 18314-18314
  13. Fazeli-Nasab, B., Rahmani, A. F., & KHajeh, H. (2021). Effects of culture medium and plant hormones in organogenesis in olive (CV. Kroneiki). J Plant Bioinform Biotech, 1(1), 1-13
  14. Gaber El-Kinany, R., Nassar, A. M. K., & El-Settawy, A. A. A. (2019). The Role of Benzyl Amino Purine and Kinetin in Enhancing the Growth and Flowering of three Gaillardia Varieties. In J. Agric. Sci, 64(5)
  15. Kasem, M., & Helaly, A. (2021). Response of Syngonium podophyllum Plant to Some Synthetic Cytokinin Types and Concentrations as a Foliar Application. Scientific Journal of Flowers and Ornamental Plants, 8(3), 321–334. https://doi.org/10.21608/sjfop.2021.198629
  16. Kieber, J. J., & Schaller, G. E. (2014). Cytokinins. The Arabidopsis Book/American Society of Plant Biologists, 12,0168
  17. Kuryata, V. G., Kushnir, О. V., & Kravets, О. О. (2020). Effect of 6-Benzylaminopurine on morphogenesis and production process of sweet pepper (Capsicum annuum L.). Ukrainian Journal of Ecology, 10(2), 106-111
  18. Lozano-Miglioli, J., Giardina, E., & Di Benedetto, A. (2019). Biomass accumulation in an ornamental Cactaceae (Mammillaria elongata subsp. echinaria) in response to a single 6-benzylaminopurine (BAP) spray. Journal of the Professional Association for Cactus Development, 21, 43-56
  19. Manokari, M., Badhepuri, M. K., Cokulraj, M., Rajput, B. S., Dey, A., Faisal, M., Alatar, A. A., Alok, A., & Shekhawat, M. S. (2022). High-throughput in vitro propagation and evaluation of foliar micro-morpho-anatomical stability in Musa acuminata cv. ‘Grand Nain’ using 6-benzoyladenine (BOA) in the nutrient medium. Scientia Horticulturae, 304. https://doi.org/10.1016/j.scienta.2022.111334
  20. Martins, J. P. R., Wawrzyniak, M. K., Kalemba, E. M., Ley-López, J. M., Mendes, M. M., Naskręt-Barciszewska, M. Z., Barciszewski, J., & Chmielarz, P. (2024). Differential morphophysiological and epigenetic responses during in vitro multiplication of Quercus robur depending on donor age and plant growth regulators. Plant Cell, Tissue and Organ Culture, 159(3). https://doi.org/10.1007/s11240-024-02914-2
  21. Meshram, A., & Srivastava, N. (2014). Molecular and physiological role of Epipremnum aureum. In International Journal of Green Pharmacy, 8(2),73–76. https://doi.org/10.4103/0973-8258.129566
  22. Meshram, A., & Srivastava, N. (2015). Epipremnum aureum (Jade pothos): a multipurpose plant with its medicinal and pharmacological properties. Journal of Critical Reviews, 2(2), 21-5
  23. Mubarok, S., Alissya, A., Drikarsa, D., Farida, F., Nuraini, A., Jaya, M. H. I. S., Rufaidah, F., & Abdulakasim, S. (2024). Combination effects of NPK fertilizer and benzyl amino purine (BAP) in accelerating Cattleya Orchid vegetative growth. Ornamental Horticulture, 30. https://doi.org/10.1590/2447-536X.V30.E242787
  24. Nuraini, A., Nugroho, P. S., Sutari, W., Mubarok, S., & Hamdani, J. S. (2021). Effects of cytokinin and paclobutrazol application time on growth and yield of G2 potato (Solanum tuberosum L.) Medians cultivar at medium altitude in Indonesia. Agriculture and Natural Resources, 55(2), 171–176. https://doi.org/10.34044/j.anres.2021.55.2.02
  25. Obiefuna, J. C., & Ndubizu, T. O. C. (1979). Estimating Leaf Area of Plantain. In Scientia Horticulturae, 11
  26. Oshchepkov, M. S., Kalistratova, A. v., Savelieva, E. M., Romanov, G. A., Bystrova, N. A., & Kochetkov, K. A. (2020). Natural and synthetic cytokinins and their applications in biotechnology, agrochemistry and medicine. Russian Chemical Reviews, 89(8), 787–810. https://doi.org/10.1070/rcr4921
  27. Osugi, A., & Sakakibara, H. (2015). Q and A: How do plants respond to cytokinins and what is their importance?. BMC Biology, 13(1). https://doi.org/10.1186/s12915-015-0214-5
  28. Peng, L., Liu, H., Wu, Y., Bing, J., & Zhang, G. (2024). New advances in the regulation of stem growth in vascular plants. In Plant Growth Regulation, 103(1), 65–80. https://doi.org/10.1007/s10725-023-01100-2
  29. Ramayana, S., Supriyanto, B., Sunaryo, W., Susylowati, S., & Adiastie, S. (2022, January). Benzyl Amino Purine (BAP) Growth Regulator Application and Shoot Origin Stem Lai (Durio kutejensis) Against Growth Durian (Durio zibethinus Murr) Grafting Seedlings. In International Conference on Tropical Agrifood, Feed and Fuel, 26-33
  30. Riefler, M., Novak, O., Strnad, M., & Schmülling, T. (2006). Arabidopsis cytokinin receptors mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism. Plant Cell, 18(1), 40–54. https://doi.org/10.1105/tpc.105.037796
  31. Singh, A. K., Sharma, M. K., Chaudhary, R., & Sengar, R. S. (2017). Effects of BAP and adenine sulphate on shoot regeneration fom callus in potato (Solanum Tuberosum L.). Biotech Today: An International Journal of Biological Sciences, 7(1), 49. https://doi.org/10.5958/2322-0996.2017.00006.0
  32. Bhupenchandra, I., Athokpam, H. S., Singh, N. B., Singh, L. N., Devi, S. H., Chongtham, S. K., Singh, L.K., Sinyorita, S., Devi, E.L, Bhagowati, S., Bora, S.S., Kumar, A., Devi, C.H.P., and Olivia, L.C. (2021). Leaf color chart (LCC): An instant tool for assessing nitrogen content in plant: A review. The Pharma Innovation Journal, 10(4), 1100-1104
  33. Skalák, J., Vercruyssen, L., Claeys, H., Hradilová, J., Černý, M., Novák, O., Plačková, L., Saiz-Fernández, I., Skaláková, P., Coppens, F., Dhondt, S., Koukalová, S., Zouhar, J., Inzé, D., & Brzobohatý, B. (2019). Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis. The Plant Journal, 97(5), 805-824
  34. Sosnowski, J., Malinowska, E., Jankowski, K., Król, J., & Redzik, P. (2019). An estimation of the effects of synthetic auxin and cytokinin and the time of their application on some morphological and physiological characteristics of Medicago x varia T. Martyn. Saudi Journal of Biological Sciences, 26(1), 66–73. https://doi.org/10.1016/j.sjbs.2016.12.023
  35. Tang, Y., Fang, Z., Liu, M., Zhao, D., & Tao, J. (2020). Color characteristics, pigment accumulation and biosynthetic analyses of leaf color variation in herbaceous peony (Paeonia lactiflora Pall.). 3 Biotech, 10(2). https://doi.org/10.1007/s13205-020-2063-3
  36. Tubić, L., Savić, J., Mitić, N., Milojević, J., Janošević, D., Budimir, S., & Zdravković-Korać, S. (2016). Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.). Plant Cell, Tissue and Organ Culture, 124(1), 1–14. https://doi.org/10.1007/s11240-015-0869-1
  37. Wang, F., Yu, Z., Zhang, M., Wang, M., Lu, X., Liu, X., Li, Y., Zhang, X., Tan, B. cai, Li, C., & Ding, Z. (2022). ZmTE1 promotes plant height by regulating intercalary meristem formation and internode cell elongation in maize. Plant Biotechnology Journal, 20(3), 526–537. https://doi.org/10.1111/pbi.13734
  38. Werner, S., Bartrina, I., & Schmülling, T. (2021). Cytokinin regulates vegetative phase change in Arabidopsis thaliana through the miR172/TOE1-TOE2 module. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-26088-z
  39. Werner, T., Nehnevajova, E., Köllmer, I., Novák, O., Stmad, M., Krämer, U., & Schmülling, T. (2010). Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco. Plant Cell, 22(12), 3905–3920. https://doi.org/10.1105/tpc.109.072694
  40. Wu, W., Du, K., Kang, X., & Wei, H. (2021). The diverse roles of cytokinins in regulating leaf development. In Horticulture Research, 8(1). https://doi.org/10.1038/s41438-021-00558-3
  41. Yin, Y., Zhong, R., Li, Y., Guo, B., Li, L., Ma, G., Wu, K., Fang, L., & Zeng, S. (2025). BAP regulates lateral bud outgrowth to promote tillering in Paphiopedilum callosum (Orchidaceae). BMC Plant Biology, 25(1), 241. https://doi.org/10.1186/s12870-025-06256-9
  42. Zhang, Z., Zhang, Y., Zhang, S., Wang, L., Liang, X., Wang, X., Wu, H., Zou, H., Zhang, C., & Wang, M. (2022). Foliar Spraying of 6-Benzylaminopurine Promotes Growth and Flavonoid Accumulation in Mulberry (Morus alba L.). Journal of Plant Growth Regulation, 41(6), 2232–2245. https://doi.org/10.1007/s00344-021-10435-x

Last update:

No citation recorded.

Last update:

No citation recorded.