DOI: https://doi.org/10.14710/baf.10.1.2025.55-62

Struktur Histologi Duodenum Tikus Putih (Rattus norvegicus) setelah Pemberian Sediaan Nanokitosan Ekstrak Etanol Daun Mimba (Azadirachta indica A. juss)

Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Jl. Prof. Jacob Rais Tembalang, Semarang, 50275, Indonesia

Open Access Copyright 2025 Buletin Anatomi dan Fisiologi

Citation Format:
Abstract

Mimba merupakan tanaman obat yang umumnya dikonsumsi secara oral, konsumsi obat secara oral memiliki tingkat bioavaibilitas yang rendah dalam tubuh. Nanokitosan mampu mengenkapsulasi senyawa aktif dan mendistribusikannya ke sirkulasi sistemik serta meminimalisir efek toksik. Tujuan dari penelitian ini adalah menganalisis pengaruh paparan sediaan nanokitosan ekstrak etanol daun mimba (Azadirachta indica A. juss) terhadap struktur histologi duodenum tikus putih (Rattus norvegicus) betina. Metode penelitian menggunakan desain Rancangan Acak Lengkap (RAL) terdiri atas 3 perlakuan dan 4 pengulangan. Perlakuan terdiri dari P0 (kontrol), P1 (ekstrak etanol daun mimba), dan P2 (nanokitosan ekstrak etanol daun mimba) masing-masing perlakuan diberikan dalam 2 ml/ekor/hari selama 21 hari. Variabel yang diukur berupa diameter duodenum, tebal lapisan muskularis, panjang vili, dan skor kerusakan deskuamasi vili melalui skoring dengan skor 0-3 Data dianalisis secara statistik parametrik dengan ANOVA pada taraf kepercayaan 95. Hasil penelitian menunjukkan bahwa diameter duodenum, tebal lapisan muskularis, dan panjang vili tidak berbeda nyata (P>0,05). Skor kerusakan deskuamasi vili menunjukkan P0 menunjukkan vili normal (skor 0), P1 terdapat kerusakan sedang (skor 2), dan P2 terdapat kerusakan ringan (skor 1). Kesimpulan menunjukkan bahwa sediaan ekstrak etanol daun mimba dalam bentuk nanopartikel mampu memberikan efek proteksi terhadap kerusakan struktur duodenum tikus.

 

Neem is a medicinal plant that is generally consumed orally, oral conpsumption of drugs has a low level of bioavailability in the body. Nanochitosan able to encapsulate active compounds and distributing them throughout the systemic circulation and minimize toxic effects.The purpose of this study was to analyze the effect of treatment of nanochitosan ethanol extract of neem leaves (Azadirachta indica A. juss) on the histological structure of the duodenum of female white rats. This study used a Completely Randomized Design (CRD) consisted of 3 treatments and 4 replication. The treatments was devided into P0 (control), P1 (ethanol extract of neem leaves), and P2 (nanochitosan ethanol extract of neem leaves), each treatment was given 2 ml/head/day for 21 days. The variables measured were duodenal diameter, muscularis layer thickness, villi length, and villi desquamation damage score by scoring with scores 0-3. Data were analyzed with ANOVA at a confidence level of 95%. The results showed that the duodenum diameter, muscularis layer thickness and villi length had no significant difference (p>0,05). The villi desquamation damage score P0 showed normal villi (score 0), P1 had moderate damage (score 2), and P2 had slight damage (score 1). The conclusion shows that treatment of neem leaf ethanol extract in the form of nanoparticles was able to protect the structure of the rat duodenum from damage.

Note: This article has supplementary file(s).

Fulltext View|Download |  common.other
Untitled
Subject
Type Other
  Download (B)    Indexing metadata
 Research Results
Aurel Elizabeth Darsono_24020120130087_Gambar dan EC
Subject
Type Research Results
  Download (1MB)    Indexing metadata
Keywords: deskuamasi; enkapsulasi; mimba; vili
Funding: Fakultas Sains dan Matematika Universitas Diponegoro under contract 30191D/UN7.5.8.2/PG/2022

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Peningkatan Pertumbuhan Tajuk dan Nilai Estetika Tanaman Hias Epipremnum aureum pada Aplikasi Sitokinin Sintetik (6-benzylaminopurine) Profil Eritrogram Itik Peking (Anas platyrhynchos domesticus) Setelah Penambahan Tepung Spirulina (Arthrospira platensis) Sebagai Suplemen Pakan Histomorfometri Jantung dan Aorta Itik Hibrida yang Diberi Pakan Imbuhan Tepung Daun Kelor (Moringa oleifera Lam.) More recent articles
  1. Alfina, Siti., Febriani, H., Syukriah. (2022). Uji Efektivitas Ekstrak Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Epitel Duodenum Tikus Putih Jantan (Rattus norvegicus) yang Diinduksi Aspirin. Journal of Agromedicine and Medical Sciences 8(2): 108-114
  2. Andayani, Ni. P. S, Setyawati, I., Sudatri, N. W. (2023). Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomatch and Duodenum in Mice. Jurnal Riset Biologi dan Aplikasinya 5(2): 52-59
  3. Eliziyad, M. T., Widjaja, N. M., Plumeriastuti, H. (2013). Pengaruh Boraks terhadap Gambaran Histopatologi Duodenum Tikus Putih (Rattus norvegicus). Veterinaria Medika, 6(2): 133-138
  4. Escobar, O. R., Castañeda, P. S., Almazán, E. A., Durán, A. V., Juárez, M. P., Graziano, R. V., Florido, M. I. M., Perez, B. R., Cruz, I. M. R., Calderon, J. E. M., Chávez, J. J. (2023). Chitosan Nanoparticles as Oral Drug Carriers. International Journal of Molecular Sciences 24(5): 1-17
  5. Fitri, Dwiki., Kiromah, N. Z., Widiastuti, T. C. (2020). Formulasi Dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Salam (Syzygium polyanthum) pada Berbagai Variasi Komposisi Kitosan dengan Metode Gelasi Ionik. Journal of Pharmaceutical Science and Clinical Research 5(1): 61-69
  6. Handoyo, Diana. L., Pranoto, M. E. (2020). Pengaruh Variasi Suhu Pengeringan terhadap Pembuatan Simplisia Daun Mimba (Azadirachta indica). Jurnal Farmasi Tinctura 1(2): 45-54
  7. He, X., Hwang, H. M. (2016). Nanotechnology in Food Science: Functionality, Applicability, and Safety Assessment. Journal of Food and Drug Analysis 24(4): 671-681
  8. Hugenholtz, F., Vos, W. M. (2018). Mouse Models for Human Intestinal Microbiota Research: a Critical Evaluation. Cellular and Molecular Life Sciences 75(7402): 149-160
  9. Kusuma, A. B., Saraswati, T. R., Sitasiwi, A. J. (2019). Efek Pemberian Daun Mimba (Azadirachta indica) terhadap Diameter Hepatosit Tikus (Rattus norvegicus). Bioma 21(2): 106-113
  10. Mawaddah, H. S., Saragih, H. T. (2020). Aktivitas Ekstrak Etanolik Daun Jambu Mete Terhadap Otot Pectoralis Thoracicus Ayam Jawa Super. Jurnal Peternakan Indonesia 22(1): 80-88
  11. Noviana, Dian. S. (2023). The Effect of Honey Administration on The Histopathology of The Duodenum of Wistar Rats as a Inhibition of The Toxic Effects of Borax (Sodium tetraborate). Internasional Islamic Medical Journal 4(2): 28-36
  12. Otto, G. M., Franklin, C. L., Clifford, C. B. (2015). Laboratory Animal Medicine (Third Edition) . Chester, New Hampshire: American College of Laboratory Animal Medicine
  13. Putri, Fauziah. M., Sitasiwi, A. J., Isdadiyanto, S., Mardati, S. M. (2023). Profil Leukosit Tikus Jantan (Rattus novergicus L.) Galur Sprague Dawley setelah Paparan Nanokitosan Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss.). Jurnal Sains Verteriner 41(1): 29-36
  14. Seyyedin, S., Nazem, M. N. (2017). Histomorphometric Study of The Effect of Methionine on Small Intestine Parameters in Rat: an Applied Histologic Study. Folia Morphol 76(4): 620-629
  15. Simbolon, Juli. E., Isdadiyanto, S., Sitasiwi, A. J. (2022). The Effect of Nanochitosan Preparation of Neem Leaf (Azadirachta indica) Ethanol Extract on the Liver Structure of White Rats (Rattus norvegicus). The International Journal of Health, Education and Social 5(6): 21-31
  16. Sitasiwi, Agung. J., Isdadiyanto, S., Mardiati, S.M. (2017). The Estradiol 17-B Concentration in Mice after Treated with Ethanolic Leaf Extract of Azadirachta indica (neem). In AIP Conference Proceeding 1844(1), AIP Publishing
  17. Sitasiwi, Agung. J. (2018). Bobot Badan Mencit (Mus musculus L.) setelah Pemberian Ekstrak Ethanol Daun Mimba (Azadirachta indica) secara Oral Selama 21 Hari. Buletin Anatomi dan Fisiologi 3(1): 133-139
  18. Sobolewska, Adrianna., Elminowska-Wenda, G., Bogucka, J., Dankowiakowska, A., Kułakowska, A., Szczerba, A., Stadnicka, K., Szpinda, M., Bednarczyk, M. (2017). The Influence of in Ovo Injection with the Prebiotic DiNovo® on the Development of Histomorphological Parameters of the Duodenum, Body Mass and Productivity in Large-scale Poultry Production Conditions. Journal of Animal Science and Biotechnology 8(45): 2-8
  19. Vdoviaková , K., Petrovová , E., Maloveská , M., Krešáková , L., Teleky, J., Elias, M. Z., Petrášová, D. (2016). Surgical Anatomy of the Gastrointestinal Tract and Its Vasculature in the Laboratory Rat. Hindawi Publishing Corporation 3(1): 1-11
  20. Yuto, K. (2021). Intestinal Villus Structure Contributes to Even Shedding of Epithelial Cells. Biophysical Journal 120(4): 699-710
  21. Zulfa, Laili. F., Sunarno, Kasiyati, & Djaelani, M. A. (2020). Efek Tepung Daun Moringa oleifera terhadap Struktur Mikroanatomi Duodenum Itik Pegging. Media Bina Ilmiah 14(9): 3135-3150

Last update:

No citation recorded.

Last update:

No citation recorded.