DOI: https://doi.org/10.14710/baf.7.2.2022.126-134

Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin.

Program Studi Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Jl. Prof. Jacob Rais, Tembalang, Semarang, Indonesia 50275, Indonesia

Open Access Copyright 2022 Buletin Anatomi dan Fisiologi

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Abstract

Prevalensi prediabetes meningkat setiap tahunnya dan diprediksi lebih dari 470 juta orang mengalami prediabetes pada tahun 2030. Induksi insulin eksogen secara terus-menerus menyebabkan resistensi insulin yang berujung pada kenaikan kadar glukosa darah yang dapat berefek pada histologi ren. Penelitian ini bertujuan untuk mengetahui gambaran histologi ren yang meliputi bobot ren, tebal kapsula Bowman, ukuran sel tubulus proksimal, ukuran sel tubulus distal, dan indeks ren setelah diinduksi dengan insulin eksogen. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Biologi Sktruktur dan Fungsi Hewan dan di kandang hewan uji, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro. Penelitian ini menggunakan 21 ekor tikus putih yang dibagi menjadi 3 kelompok perlakuan dan 7 ulangan. P0 (kontrol), P1 (diiduksi insulin glargine 1,80 IU/200gBB/hari selama 14 hari), dan P2 (diinduksi insulin glargine 1,80 IU/200gBB/hari selama 28 hari).  Insulin glargine diinjeksikan melalui subkutan dengan menggunakan xiulin pen. Preparat histologi ren dibuat dengan metode parafin dan pewarnaan Hematoksilin-Eosin. Pengamatan preparat menggunakan alat fotomikrograf.  Data penelitian kemudian dianalisis dengan uji Anova. Hasil uji Anova menunjukkan antar perlakuan berbeda tidak nyata. Kesimpulan dari penelitian ini adalah induksi insulin 1,80 IU/200gBB/hari selama 14 hari dan 28 hari tidak berpotensi menyebabkan perubahan pada struktur histologi ren tikus putih.

 

The prevalence of prediabetes increases every year and it is predicted that more than 470 million people will experience prediabetes in 2030. Continuous exogenous insulin induction causes insulin resistance which leads to an increase in blood glucose levels which can have an effect on renal histology. This study aims to determine the histology of the ren, which includes renal weight, Bowman's capsule thickness, proximal tubular cell size, distal tubular cell size, and ren index after being induced with exogenous insulin. This research was carried out for 6 months at the Laboratory of Structural and Functional Biology of Animals and in the cages of test animals, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. This study used 21 white rats which were divided into 3 treatment groups and 7 replications. P0 (control), P1 (induced by insulin glargine 1.80 IU/200gBW/day for 14 days), and P2 (induced by insulin glargine 1.80 IU/200gBW/day for 28 days). Insulin glargine is injected subcutaneously using a xiulin pen. Renal histology preparations were made by paraffin method and Hematoxylin-Eosin staining. Observation of preparations using a photomicrograph. The research data were then analyzed by the Anova test. The results of the Anova test showed that the differences between treatments were not significantly different. The conclusion of this study was that insulin induction of 1.80 IU/200gBW/day for 14 days and 28 days did not have the potential to cause changes in the histological structure of the white rat's kidney.

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Keywords: insulin glargine; prediabetes; resistensi insulin

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  13. Lestari, A. A. W. (2011). Resistensi Insulin: Definisi, Mekanisme dan Pemeriksaan Laboratoriumnya. Buku Ilmiah Clinical Pathology Update on Suramade, 1(2), 1-8. https://repositori.unud.ac.id/protected/storage/upload/repositori/ad31ce278a7564c52f74b34e9c5fa38e.pdf
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  23. Schlondorff, D., and Banas, B. (2009). The Mesangial Cell Revisited: No Cells is an Island. Journal of the American Society of Nephrology, 20(6), 1179-1187. https://doi.org/doi: 10.1681/ASN.2008050549
  24. Scindia, Y. M., Deshmukh, U. S., and Bagavant, H. (2010). Mesangial Pathology in Glomerular Disease: Targets for Therapeutic Intervention. Advanced Drug Delivery Reviews, 62(14), 1337-1343. https://doi.org/ doi: 10.1016/j.addr.2010.08.011
  25. Slyne, J., Slattery, C., McMorrow, T., and Ryan, M. P. (2015). New Developments Concerning the Proximal Tubule in Diabetic Nephropathy: in vitro models and mechanism. Nephrol Dial Transplant, 30(4), 60-67. https://doi.org/doi: 10.1093/ndt/gfv264
  26. Surasa, N. J., Utami, N. R., dan Isnaeni, W. (2014). Struktur Mikroanatomi Hati dan Kadar Kolesterol Total Plasma Darah Tikus Putih Strain Wistar Pasca Suplementasi Minyak Lemuru dan Minyak Sawit. Biosaintifika, 6(2), 141-151. https://doi.org/10.15294/biosaintifika.v6i2.3778
  27. Tabak, A. G., Herder, C., Rathmann, W., Brunner, E. J., and Kivimaki, M. (2012). Prediabetes : a High-Risk State for Diabetes Development. Lancet, 16(379), 2279-90. https://doi.org/doi: 10.1016/S0140-6736(12)60283-9
  28. Tjandrawinata, R. R. (2016). Patogenesis Diabetes Tipe 2: Resistensi Insulin dan Defisiensi Insulin. Dexa Medica, 29(1), 1-5. https://www.researchgate.net/publication/292615802_Patogenesis_Diabetes_Tipe_2_ Resistensi_Insulin_dan_Defisiensi_Insulin/citations
  29. Vallon, V. (2011). The Proximal Tubule in the Pathophysiology of the Diabetic Kidney. American Journal Physiology Regulatory Integrative Comparative Physiology, 300(5), 1009-1022. https:// DOI: 10.1152/ajpregu.00809.2010
  30. Vallon, V. and Thomson, S. C. (2012). Renal Function in Diabetic Disease Models: the Tubular System in the Pathophysiology of the Diabetic Kidney. Annual Review of Psychology, 74, 75-351. https;//doi: 10.1146/annurev-physiol-020911-153333
  31. Vallon, V., and Komers, R. (2011). Pathophysiology of the Diabetic Kidney. Comprehensive Physiology, 1(3), 232-1175. https://10.1002/cphy.c100049
  32. Zafar, M. and Naqvi, S. N. H. (2010). Effects of STZ-Induced Diabetes on the Relative Weights of Kidney, Liver and Pancreas in Albino Rats: A Comparative Study. International Journal of Morphology, 28(1), 135-142. https://doi.org/10.4067/S0717-95022010000100019

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