DOI: https://doi.org/10.14710/baf.7.2.2022.75-82

Pengaruh Paparan Aerosol Rokok Elektrik Terhadap Profil Hematologis Rattus norvegicus

Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A Surakarta, 57126 Indonesia, Indonesia

Open Access Copyright 2022 Buletin Anatomi dan Fisiologi (Bulletin Anatomy and Physiology)

Citation Format:
Abstract

 

Rokok elektrik (RE) merupakan inovasi terbaru rokok yaitu tanpa proses pembakaran tar dan tembakau dan dianggap lebih aman dibandingkan rokok konvensional. Penelitian ini bertujuan mengetahui pengaruh paparan aerosol RE terhadap profil hematologis tikus putih (Rattus norvegicus) yang meliputi kadar hemoglobin, eritrosit, leukosit serta jenis leukosit. Dalam penelitian ini hewan uji Rattus norvegicus dibagi menjadi 4 kelompok dengan perlakuan yang berbeda yaitu diberi 0x paparan/hari (kontrol); 1x paparan/hari, 2x paparan/hari, dan 3x paparan/hari selama 14 hari. Setiap paparan dilakukan selama 5 menit (10 kali hembusan). Sampel darah diambil pada hari ke-0, hari ke-7 dan ke-14 melalui sinus orbitalis. Jumlah eritrosit dan leukosit dihitung dengan metode hitung bilik Neubauer. Kadar hemoglobin ditentukan menggunakan metode Sahli, dan persentase jenis leukosit dihitung dari preparat apus darah dengan pewarnaan Giemsa. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan terjadi peningkatan kadar hemoglobin dan jumlah eritrosit yang sejalan dengan peningkatan frekuensi dan durasi paparan RE sebagai kompensasi dari kondisi hipoksia. Jumlah leukosit juga cenderung meningkat, tetapi peningkatannya tidak linier dengan peningkatan frekuensi dan durasi paparan RE. Persentase limfosit, monosit dan eosinofil dibandingkan kontrol cenderung meningkat sedangkan persentase neutrofil cenderung menurun. Dengan demikian paparan RE dapat mempengaruhi profil hematologis hewan uji.

 

 

 

E-cigarettes are the latest innovations of cigarettes without process of burning tar and tobacco. They are considered safer than conventional cigarettes. The purpose of this study was to determine the effect of e-cigarette aerosol exposure on the hematological profiles of Wistar rats (Rattus norvegicus). Rats were exposed to e-cigarette aerosol in different frequencies: 0x (control); once; twice; and 3x exposure/day. The experiment was ended on day 14th. Blood samples were taken through the orbital sinus on the day 0, 7th and 14th. Erythrocyte and leucocyte numbers were measured in Neubauer chamber count. Hemoglobin levels was determined by using Sahli method, and the percentage of leukocyte types was calculated based on Giemsa stained blood smear preparations. Data were analyzed descriptively. The results showed that hemoglobin levels and erythrocyte numbers tend to increase in line to the e-cigarette aerosol exposure as a compensation for hypoxic conditions. The leukocyte numbers also tends to increase, although the increase is not in line to the increase of exposure frequency and duration. The percentage of lymphocytes, monocytes and eosinophils tend to increase compared to the control group, while the percentage of neutrophils tends to decrease. Thus e-cigarette aerosol  exposure affected the haematological profiles of rats 


Fulltext View|Download
Keywords: profil hematologis; rokok elektrik; hemoglobin; eritrosit; leukosit

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin. Pengaruh Paparan Aerosol Rokok Elektrik Terhadap Profil Hematologis Rattus norvegicus Daya Akumulasi Kadmium (Cd) Tanaman Mangrove Rhizophora mucronata (Lamk.) di Perairan Laut dan Lahan Tambak Mangunharjo, Kecamatan Tugu, Kota Semarang More recent articles
  1. Barrett, K.E., Barman, S.M., Boitano, S., & Brooks, H.L. (2015). Buku Ajar Fisiologi Kedokteran Ganong (Edisi 24). Jakarta: EGC
  2. Blaha, M.J. & Ratchfor, E.V. 2019. Electronic Cigarettes. Vascular Medicine 24(3): 267-269. https://doi.org/10.1177/1358863X19837361
  3. Brown, B.A. (1993). Hematology Principles and Procedures. Philadelphia: Lea and Febiger Publishing
  4. Douglas, J.W., Wardrop, K.J. 2022. Schalm’s Veterinary Hematology. 7th ed. Wiley-Blackwell
  5. Forget, B.G., Bunn, A.F. 2013. Classification of the Disorders of Hemoglobin. Cold Spring Harbor Perspective in Medicine 2013: 1-11. doi: 10.1101/cshperspect.a011684
  6. Gunadi, V.I., Mewo, Y.M. & Tiho, M. (2016). Gambaran Kadar Hemoglobin pada Pekerja Bangunan. Jurnal e-Biomedik 4(2): 2–7
  7. Hajek, P., Etter, J., Benowitz, N., Eissenberg, T., & McRobbie H. (2014). Electronic Cigarettes: Review of Use, Content, Safety, Effects on Smokers and Potential Form Harm and Benefit. Addiction 109(11): 1801-1810
  8. Hall, E.J. (2013). Guyton dan Hall Buku Ajar Fisiologi Kedokteran Edisi 12. Jakarta: EGC
  9. Hilmarni, H., Almahdy, A. & Helmi, A. (2015). Kajian Toksisitas Serbuk Biji Mahoni terhadap Perkembangan Tingkah Laku, Histologi Hati serta Hematologi Anak Mencit. Jurnal Farmasi Sains dan Klinis 2(1): 15-21
  10. IARC [International Agency for Research on Cancer]. (2006). IARC Monographs On The Evaluation of Carcinogenic Risks To Humans: Formaldehyde 2 Butaxyethanol and 1-tert-Butoxypropan-2-ol. WHO: Lyon Inc
  11. Kuntic, M., Oelze, M., Steven, S., Kröller-Schön, S., Stamm, P., Kalivonic, S., Frensis, K., Vujacic-Mirski, K., Jimenez, M.T.B., Kvandova, M., Filippou, K., Al-Zuabi, A., Brückl, V., Hahad, O., Daub, S., Varveri, F., Gori, T., Huesmann, R., Hoffman, T., Schmidt, F.P., Keaney, J.F., Daiber, A., & Münzel, T. (2019). Short-Term E-Cigarette Vapour Exposure Causes Vascular Oxidative Stress and Dysfunction: Evidence for A Close Connection To Brain Damage and Key Role of The Phagocytic NADPH Oxidase (NOX-2). European Heart Journal 41(26): 2472-2483
  12. Leick, M., Azcutia, V. & Newton, G. 2014. Leukocyte Recruitment in Inflammation: Basic Concepts and New Mechanistic Insights Based on New Models and Microscopic Imaging Technologies. Cell and Tissue Research 355(3): 647–656. doi: 10.1007/s00441-014-1809-9
  13. McCauley, L., Markin, C. & Hosmer, D. (2012). An Unexpected Consequence of Electronic Cigarette Use. Chest Postgrad Education Center Journal 141(4): 1110-1113
  14. Mishra, A., Chaturvedi, P., Datta, S., Sinukumar, S., Joshi, P., & Garg, A. (2019). Harmful Effects of Nicotine. Indian Journal of Medical and Paediatric Oncology 36(1): 24-29
  15. Muthumalage,. T, Prinz, M., Ansah, K.O., Gerloff, J., Sundar, I.K., Rahman, I. (2018). Inflammatory and Oxidative Responses Induced by Exposure to Commonly Used e-Cigarette Flavoring Chemicals and Flavored e Liquids without Nicotine. Fronties in Physiologi 8(1): 1-9
  16. Murray, R.K., Granner, D.K. & Rodwell, V.W. (2017). Biokimia Harper Edisi 30. Jakarta: EGC
  17. Notopoero, P.B. (2018). Eritropoitin Fisiologi, Aspek Klinik, dan Laboratorik. Indonesian Journal of Clinical Pathology and Medical Laboratory 14(1): 28-36
  18. Prenggono, M.D. (2015). Eritropoiten dan Penggunaan Eritropoiten pada Pasien Kanker dengan Anemia. Jurnal Cermin Dunia Kedokteran 4(1): 20-28
  19. Singh, R.B., Mengi, S.A., Xu, Y.J., Arneja, A.S., & Dhalla, N.S. (2002). Pathogenesis of Atherosclerosis: A Multifactorial Process. Experimental and Clinical Cardiology 7(1): 41-53
  20. Sitasiwi, A.J. & Isdadiyanto, S. (2017). Kadar Hemoglobin dan Jumlah Eritrosit Mencit (Mus musculus) Jantan setelah Perlakuan dengan Ekstrak Etanol Daun Nimba (Azadirachta indica). Buletin Anatomi dan Fisiologi 2(2): 161-167
  21. Son, Y., Bhattarai, C., Samburova, V., & Khlystov, A. (2020). Corbonyls and Carbon Monoxide Emissions from ilectronic Cigarettes Affected by Devices Type and Use Patterns. International Journal of Environmental Research and Public Health 17(2726): 115
  22. Sumiasih, N.Y. & Budiani, N.Y. (2016). Biologi Dasar dan Biologi Perkembangan. Jakarta: Kementerian Kesehatan RI
  23. Tanuwihardja, R.K. & Susanto, A.D. (2012). Rokok Elektrik. Jurnal Respirasi Indonesia 32(1): 53-61

Last update:

No citation recorded.

Last update:

No citation recorded.