skip to main content

Pengaruh Perendaman dalam Chitosan Nanoparticles Daun Bidara Arab (Ziziphus spina-christi) terhadap Profil Darah dan Pertumbuhan Ikan Patin (Pangasius sp.) yang diinfeksi A. hydrophila

Departemen Akuakultur, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

Open Access Copyright 2023 Narita Reci Kusuma, Sarjito Sarjito, Desrina Desrina

Citation Format:
Abstract
Permasalahan yang seringkali ditemui pada budidaya ikan patin adalah adanya penyakit Motile Aeromonas Septicemia (MAS). Penyakit tersebut dapat disebabkan oleh bakteri Aeromonas hydrophila. Penanganan A. hydrophila yang masih mengandalkan peran antibiotik yang tidak ramah lingkungan dan apabila digunakan secara terus menerus akan menimbulkan residu pada ikan dan resistensi bakteri. Oleh karena itu diperlukan alternatif pengobatan penyakit MAS yang ramah lingkungan dan aman bagi ikan, salah satunya dengan Chitosan Nanoparticles (CNPs) daun bidara arab. Penelitian ini bertujuan untuk mengkaji pengaruh CNPs daun bidara arab (Ziziphus spina-christi) terhadap profil darah Pangasius sp. yang diinfeksi bakteri A. hydrophila dilakukan perendaman pada ikan uji. Dosis perendaman untuk perlakuan uji pada masing-masing perlakuan, yaitu A (0 ppm), B (450 ppm), C (500 ppm), D (550 ppm) dan kontrol positif berupa tetrasiklin (10 ppm). Setelah dilakukan perendaman dengan waktu pemeliharaan selama 14 hari, dilakukan perhitungan pada total eritrosit, hemoglobin (Hb), pertumbuhan bobot mutlak dan pengamatan gejala klinis. Hasil yang didapatkan menunjukkan bahwa ikan patin yang telah diinfeksi A. hydrophila menunjukkan gejala klinis berupa penurunan nafsu makan, ulcer (borok), bercak merah, eksoptalmia, pigmentasi warna kulit, dan sirip geripis. Hasil perendaman CNPs daun bidara arab tidak berpengaruh nyata (P>0,05) terhadap, total eritrosit, hemoglobin (Hb), serta pertumbuhan bobot mutlak. Secara keseluruhan, hasil terbaik perendaman menggunakan  CNPs daun bidara arab pada ikan patin yang diinfeksi A. hydrophila pada perlakuan C (500 ppm).
Fulltext View|Download

Article Metrics:

  1. Abdelhamed, Banes M., Karsi A., and Lawrence, M. “Recombinant ATPase of Virulent Aeromonas Hydrophila Protects Channel Catfish Against Motile Aeromonas Septicemia”. Frontiers In Immunology, no. 10 (2019): 1641
  2. Ali, Medyan N., Tagreed M. Al-Saadi, and Jamal KA AL-Faragi. "Effect of Chitosan Nanoparticles Loaded Oxytetracycline Hydrochloride on Health Status of Common Carp (Cyprinus carpio L.) Infected with Columnaris Disease." In Journal of Physics: Conference Series, vol. 1879, no. 3, p. 032075. IOP Publishing, 2021
  3. Anderson, D.P. 1974. T.R.H. Publication. Inc. Ltd. Hongkong. 239 p
  4. Anju A., Nagda G., and Chauhan D.K. Haematological Alterations in Kuroiler Chicks Exposed to Cadmium Acetate. Advances in Zoology and Botany 8, no.3 (2020): 181-187
  5. Aparna, V., Dileep, K. V., Mandal, P. K., Karthe, P., Sadasivan, C., & Haridas, M. (2012). Anti-inflammatory Property of n-hexadecanoic acid: Structural Evidence and Kinetic Assessment. Chemical Biology and Drug Design, 80(3), 434–439. https://doi.org/10.1111/j.1747- 0285.2012.01418.x
  6. Asimuddin M., Shaik M.R., Fathima N., Afreen M., Adil S., Siddiqui M.R., Jamil K., and Khan M. Study of Antibacterial Properties of Ziziphus Mauritiana Based Green Synthesized Silver Nanoparticles Against Various Bacterial Strains. Sustainability12, no. 1484 (2020): 1-14
  7. Bamsaoud S. F., M. M. Basuliman, E. A. Bin-Hameed, S. M. Balakhm, and A. S. Alkalali, “The Effect of Volume and Concentration of AgNO3 Aqueous Solutions on Silver Nanoparticles Synthesized using Ziziphus spina–christi Leaf Extract and Their Antibacterial Activity,” Journal of Physics: Conference Series, vol. 1900, no. 1, Article ID 012005, 2021
  8. Cerlina M., Riauwaty M., dan Syawal H. Gambaran Eritrosit Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Aeromonas hydrophila dan Diobati dengan Larutan Daun Salam (Syzygium polyantha). Jurnal Perikanan dan Kelautan 27, no.1 (2021): 105-113
  9. Davis, W.W. Stout TR: Disc Plate Methods of Microbiological Antibiotic Assay. Microbiology, 22 (1971): 659-665
  10. Dewi, Tammy Mulia. "Pengembangan Metode Analisis Tetrasiklin pada Madu di Kota Bandung." PhD diss., Matematika Dan Ilmu Pengetahuan Alam (UNISBA), 2015
  11. Doan, H. V., Doolgindachabaporn, S., & Suksri, A. The LD50 of Asian Catfish (Pangasius bocourti, Sauvage 1870) Challenge to Pathogen Aeromonas hydrophila FW52 strain. Pensee 75, no. 10 (2013): 287-293
  12. Fajriyani, Aryana, and Sri Hastuti. "Pengaruh Serbuk Jahe pada Pakan Terhadap Profil Darah, Pertumbuhan dan Kelulushidupan Ikan Patin (Pangasius sp.)." Journal of Aquaculture Management and Technology 6, no. 4 (2017): 39-48
  13. Garcia, F.; Moraes, F.R.; Martins, M.L. 2009. Challenge of Pacu (Piaractus mesopotamicus) Fed Diets Supplemented with Vitamins C and E by Aeromonas hydrophila Under Different Temperatures. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(2): 378-385
  14. Goot V.D., Françoise Gisou, Kim R. Hardie, Michael W. Parker, and J. Thomas Buckley. "The C-Terminal Peptide Produced Upon Proteolytic Activation of the Cytolytic Toxin Aerolysin is Not Involved in Channel Formation." Journal of Biological Chemistry 269, no. 48 (1994): 30496-30501
  15. Halawani E.M., “Rapid Biosynthesis Method and Characterization of Silver Nanoparticles Using Zizyphus spina christi Leaf Extract and Their Antibacterial Efficacy in Therapeutic Application,” Journal of Biomaterials and Nanobiotechnology, vol. 8, no. 1, pp. 22–35, 2017
  16. Hartika, R., Mustahal dan A. N. Putra. 2014. Gambaran Darah Ikan Nila (Oreochromis niloticus) dengan Penambahan Dosis Prebiotik yang Berbeda dalam Pakan. Jurnal Perikanan dan Kelautan, 4(4): 259-267
  17. Hungund, B.S., Dhulappanavar, G.R. and Ayachit, N.H. (2015) Comparative Evaluation of Antibacterial Activity of Silver Nanoparticles Biosynthesized Using Fruit Juices. Journal of Nanomedicine and Nanotechnology, 6, 271. https:/doi.org/10.4172/2157-7439.1000271
  18. Mahajan, R.T.; Chopda, M. Phyto-Pharmacology of Ziziphus jujuba Mill-A plant review. Pharmacogn. Rev. 2009, 3, 320
  19. Maulidya, Bella, Junianto Rosidah, and Walim Lili. "Effectivity of Sambiloto Extract as Medicine for Catfish (Pangasius hypophthalmus) Juveniles Infected by Aeromonas hydrophila." Int. J. Fish. Aquat. Stud. 5 (2017): 236-241
  20. Mubarak, A., Thajuddin, N., Jeganathan, K. and Gunasekaran, M. (2011) Plant Extract Mediated Synthesis of Silver and Gold Nanoparticles and Its Antibacterial Activity against Clinically Isolated Pathogens. Colloids and Surfaces B: Biointerfaces, 85, 360-365
  21. Nugroho RA, Fotedar R (2013) Dietary Organic Selenium Improves Growth, Survival and Resistance to Vibrio mimicus in Cultured Marron, Cherax cainii (Austin, 2002). Fish Shellfish Immun 35(1):79– 85
  22. Oliveira, S. R. D., Souza, R. T. Y. B. D., Brasil, E. M., Andrade, J. I. A. D., Nunes, É. D. S. S., Ono, E. A., & Affonso, E. G. (2011). LD50 of the Bacteria Aeromonas hydrophila to Matrinxã. Brycon amazonicus. Acta Amazonica, 41: 321-326
  23. Purnamasari, Linda. "Perendaman Ikan Lele Sangkuriang (Clarias Sp.) dalam Sari Buah Belimbing Wuluh untuk Mengobati Infeksi Aeromonas hydrophila." Jurnal Akuakultur Rawa Indonesia 3, no. 1 (2015): 82-93
  24. Ramakrishnan M. A. (2016). Determination of 50% Endpoint Titer using a Simple Formula. World journal of virology, 5(2): 85–86
  25. Riauwaty, M., Siregar, Y., & Mulyani, I. (2021). Effectiveness of Turmeric-Enriched Pellets to Improve the Immunity of Clarias Batrachus Toward Motile Aeromonas Septicemia Disease. F1000Research, 10(169): 1-16
  26. Riauwaty, M., Windarti, W., Sari, T. Y., & Adelina, A. (2019, November). Blood Condition of Pangasius hypophthalmus Fed with Turmeric Enriched Pellets and Infected with Aeromonas hydrophila. In IOP Conference Series: Earth and Environmental Science (Vol. 348, No. 1, p. 012036). IOP Publishing
  27. Rumengan, I. F., Rumampuk, N. D., Mandey, L. C., Kandou, G. D., & Luntungan, A. H. (2020, June). Antioxidant Activitiy of Bitter Leaves (Vernonia amygdalina) Extract Coated with the Nanochitosan Derived from Parrot Fish (Scarus sp.) Scales. In IOP Conference Series: Earth and Environmental Science, 517(1): 012017). IOP Publishing
  28. Sarjito, Purnamayanti, L., Riyadi, P.H., Desrina, dan Prayitno, S.B. (2021). Phytochemical Analysis and Antibacterial Activities of Sidr Leaf Extract (Ziziphus spina-christi) Against Pathogenic Bacteria in Aquaculture. Pertanika Journal of Tropical Agricultural Science, 44(4):845-864
  29. Shaalan, Mohamed, Mona Saleh, Magdy El-Mahdy, and Mansour El-Matbouli. "Recent Progress in Applications of Nanoparticles in Fish Medicine: a Review." Nanomedicine: Nanotechnology, Biology and Medicine 12, no. 3 (2016): 701-710
  30. Swamy, M. K., Arumugam, G., Kaur, R., Ghasemzadeh, A., Yusoff, M. M., & Sinniah, U. R. (2017). GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves. Evidence-Based Complementary and Alternative Medicine, 2017, 1517683
  31. Turk, E and H. Oguz. 2016. Investigation of Tetracycline Residues in Fish Caught from Surrounding Fish Farms in Muğla District. Eurasian Journal of Veterinary Sciences, 32(2): 74-79
  32. Utami, D. T., Prayitno, S. B., Hastuti, S., & Santika, A. (2013). Gambaran Parameter Hematologis pada Ikan Nila (Oreochromis niloticus) yang Diberi Vaksin DNA Streptococcus iniae dengan Dosis yang Berbeda. Journal of Aquaculture Management And Technology, 2(4): 7-20
  33. Yahia, Y., Benabderrahim, M.A., Tlili, N., Bagues, M., & Nagaz, K. (2020). Bioactive Compounds, Antioxidant and Antimicrobial Activities of Extracts from Different Plant Parts of Two Ziziphus Mill. Species. Plos ONE, 15(5): 1-16, E0232599

Last update:

No citation recorded.

Last update:

No citation recorded.