Pemanfaatan khamir inulinolitik indigenous ampas tebu (bagasse) sebagai penghasil biokatalis inulinase dalam produksi gula rendah kalori

Abstract

Low calorie sugar is an alternative sweetener with low or even zero calories that can use inulin as its raw material. Inulin is a polysaccharide that can be used as a raw material for the production of high fructose syrup (HFS) by hydrolyzing it into fructose using the inulinase. The production of inulinase in Indonesia still relies on imports and is limited. The exploration is needed to produce inulinase such as by utilizing inulinolytic yeasts obtained from bagasse. Bagasse is a material that has great potential as a carbon source in the form of inulin which can support the growth of inulinolytic yeasts. Based on the literature review, it was found a potential yeast in bagasse and had inulinolytic activity like Kluyveromyces marxianus. The part of plant as raw material that has the highest inulin content is dahlia tuber. Optimization of inulinase activity in yeast on bagasse can be done by adding Ca2+ metal ion as a cofactor. The innovative use of metal ion Ca2+ in inulinolytic yeast in bagasse has the potential to produce high amounts of enzymatic activity and the use of dahlia tubers as a substrate increases fructose levels for low calorie sugar production on industrial scale.