Departemen Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Indonesia
BibTex Citation Data :
@article{JKT1703, author = {Subagiyo Subagiyo and Muhammad Djarod and Wilis Setyati}, title = {Potensi Ekosistem Mangrove Sebagai Sumber Bakteri Untuk Produksi Protease, Amilase Dan Selulase}, journal = {Jurnal Kelautan Tropis}, volume = {20}, number = {2}, year = {2017}, keywords = {bacteria; mangrove; protease; amylase; cellulose}, abstract = { The mangrove ecosystem is a productive ecosystem. The production and accumulation of organic matter on the floor of the mangrove ecosystem allows this region to be rich in microbials that are potential to be developed as a source of a wide range of extra cellular enzymes. In this research, bacteria were isolated and used as the source of enzyme. The bacteria were isolated from the existing mangrove sediments on the north coast of Semarang, Central Java. The bacterial potential as a source of protease, amylase and cellulase is done semiquantitatively using well diffusion agar method. The results obtained 18 bacteria isolates which were all capable to produce amylase and cellulase, but only 7 isolates were able to produce proteases. Five isolates had amylase activity above 300 mm2 / ml, three isolates with protease activity above 300 mm2 / ml and 2 isolates with cellulase activity of 225.40 and 204.50 mm2 / ml. Seven isolates are capable of producing amylase, protease and cellulase. The results showed that mangrove ecosystem is a potential source of microbes to produce enzymes protaese, amylase and cellulase and also possible for various other extracellular enzymes. Ekosistem mangrove merupakan ekosistem produktif. Produksi dan akumulasi bahan organik di lantai ekosistem mangrove memungkinkan daerah ini kaya akan mikrobia yang potensial untuk dikembangkan sebagai sumber berbagai macam enzim ekstra seluler. Pada penelitian ini dilakukan isolasi bakteri sebagai sumber enzim. Bakteri diisolasi dari sedimen mangrove yang ada di pantai Utara Semarang, Jawa Tengah. Pengujian potensi bakteri ini sebagai sumber protease, amilase dan selulase dilakukan secara semikuantitatif menggunakan metode well diffusion agar . Hasil penelitian mendapatkan 18 isolat bakteri yang seluruhnya mampu memproduksi amylase dan selulase, tetapi hanya 7 isolat yang mampu memproduksi protease. Lima isolat memiliki aktivitas amylase diatas 300 mm 2 /ml, tiga isolat dengan aktivitas protease diatas 300 mm 2 /ml dan 2 isolat dengan aktivitas selulase yaitu sebesar 225,40 dan 204,50 mm 2 /ml. Tujuh isolat yang mampu menghasilkan amylase, protease dan selulase. Hasil penelitian menunjukkan bahwa ekosistem mangrove merupakan sumber potensial mikrobia untuk menghasilkan enzim protaese, amylase dan selulase serta juga dimungkinkan untuk berbagai macam ensim ekstraseluler lainnya. }, issn = {2528-3111}, pages = {106--111} doi = {10.14710/jkt.v20i2.1703}, url = {https://ejournal2.undip.ac.id/index.php/jkt/article/view/1703} }
Refworks Citation Data :
The mangrove ecosystem is a productive ecosystem. The production and accumulation of organic matter on the floor of the mangrove ecosystem allows this region to be rich in microbials that are potential to be developed as a source of a wide range of extra cellular enzymes. In this research, bacteria were isolated and used as the source of enzyme. The bacteria were isolated from the existing mangrove sediments on the north coast of Semarang, Central Java. The bacterial potential as a source of protease, amylase and cellulase is done semiquantitatively using well diffusion agar method. The results obtained 18 bacteria isolates which were all capable to produce amylase and cellulase, but only 7 isolates were able to produce proteases. Five isolates had amylase activity above 300 mm2 / ml, three isolates with protease activity above 300 mm2 / ml and 2 isolates with cellulase activity of 225.40 and 204.50 mm2 / ml. Seven isolates are capable of producing amylase, protease and cellulase. The results showed that mangrove ecosystem is a potential source of microbes to produce enzymes protaese, amylase and cellulase and also possible for various other extracellular enzymes.
Ekosistem mangrove merupakan ekosistem produktif. Produksi dan akumulasi bahan organik di lantai ekosistem mangrove memungkinkan daerah ini kaya akan mikrobia yang potensial untuk dikembangkan sebagai sumber berbagai macam enzim ekstra seluler. Pada penelitian ini dilakukan isolasi bakteri sebagai sumber enzim. Bakteri diisolasi dari sedimen mangrove yang ada di pantai Utara Semarang, Jawa Tengah. Pengujian potensi bakteri ini sebagai sumber protease, amilase dan selulase dilakukan secara semikuantitatif menggunakan metode well diffusion agar. Hasil penelitian mendapatkan 18 isolat bakteri yang seluruhnya mampu memproduksi amylase dan selulase, tetapi hanya 7 isolat yang mampu memproduksi protease. Lima isolat memiliki aktivitas amylase diatas 300 mm2/ml, tiga isolat dengan aktivitas protease diatas 300 mm2/ml dan 2 isolat dengan aktivitas selulase yaitu sebesar 225,40 dan 204,50 mm2/ml. Tujuh isolat yang mampu menghasilkan amylase, protease dan selulase. Hasil penelitian menunjukkan bahwa ekosistem mangrove merupakan sumber potensial mikrobia untuk menghasilkan enzim protaese, amylase dan selulase serta juga dimungkinkan untuk berbagai macam ensim ekstraseluler lainnya.
Article Metrics:
Last update:
Upon acceptance for publication, authors agree to transfer the copyright of their article to Jurnal Kelautan Tropis, while retaining the right to reuse their work under the terms of the open license applied.
From the date of publication, the copyright for each article is held by Jurnal Kelautan Tropis. This transfer allows the journal to manage, disseminate, and preserve scholarly content in accordance with international standards and open access best practices.
Although copyright is held by the journal, all published articles are made available under the Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0). Under this license, anyone may:
Copy and redistribute the material in any medium or format
Remix, transform, and build upon the material for any purpose, even commercially
provided that:
Appropriate credit is given to the original author(s) and the source
Indications are made of any changes that were made
Derivative works are distributed under the same license (CC BY-SA 4.0)
While copyright is held by the journal, authors retain important reuse rights. Authors may:
Reuse the published version of their article in future works, including books, compilations, and lectures
Deposit the published version in institutional or subject repositories
Share the article freely, including on personal websites or academic networks
as long as the original publication in Jurnal Kelautan Tropis is cited and the CC BY-SA 4.0 license terms are respected.
Authors must ensure that any third-party content included in the article (e.g., figures, images, datasets) is either original, in the public domain, or licensed for reuse under compatible terms. If specific permissions are required, authors must obtain them prior to submission.
For questions regarding copyright or licensing, please contact the editorial office at: j.kelautantropis@gmail.com
View My Stats
Jurnal Kelautan Tropis is published by Departement of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro under a Creative Commons Attribution-ShareAlike 4.0 International License.
