Effect of Nigella Sativa on IL-10 in MB Leprosy that Received MDT-WHO Therapy

*Febrina Primasanti  -  dr. Kariadi Semarang Hospital, Indonesia
Lewie Suryaatmadja  -  Faculty of Medicine Diponegoro University, Indonesia
R. Sri Djoko  -  Faculty of Medicine Diponegoro University, Indonesia
Received: 8 Mar 2016; Published: 30 Jul 2016.
Open Access
Citation Format:
Article Info
Section: Original Research Articles
Language: EN
Full Text:
Statistics: 456 179


Background: Leprosy, a chronic infectious disease presents a broad clinical spectrum that is correlated with the immunological response of the patient, mainly related to Th1/Th2 cells. IL-10 is a major cytokine produced by Th2 cells inhibits imunostimulatory cytokine produced by Th1 cells. Suppressive effects of IL-10 in monocytes and cytokine synthesis by Th1 cells presumably because IL-10 has a general suppressive effect on immune function. Nigella sativa has a potent potentiating effect on cellular immunity through suppression of Th2 cells and IL-10, resulting in potentiation of Th1 cells.
: The study design is a randomized pretest and posttest controlled design involving 40 subjects of MB leprosy patients. Serum levels of IL-10 were measured by ELISA.
Result: The mean decrease in serum levels of IL-10 (IL-10 delta) in the treatment group (average fell 3.12 pg/ml) is greater than the control group (average rose 0.21 pg/ml), where the difference is statistically significant (p = 0.029). Nigella sativa giving significant correlation with a decrease in IL-10 compared to the control group (p=0.044, OR: 10.23).
Conclusion: supplementation of Nigella sativa 3 x 1000 mg for 2 months in patients with MB leprosy can reduce levels of IL-10, thus increasing the cellular immune response in patients with MB leprosy.

MB Leprosy; Nigella sativa; IL-10

Article Metrics:

  1. Eichelmann K, Gonzalez SE, Salas-Alanis JC, Ocampo-Candiani J. Leprosy. An update: definition, pathogenesis, classification, diagnosis, and treatment. Actas Dermosifiliogr. 2013;104(7):554-63.
  2. Lee DJ, Rea TH, Modlin RL. Leprosy. In: Goldsmith LA, Katz SI, Gilchrest BA, Paller AS, Leffell DJ, Wolff. Fitzpatrick’s dermatology in general medicine. 8th ed. Volume 1. New York: McGraw-Hill. 2012. P. 2253-62.
  3. Abbas AK, Lichtman AH, Pober JS. Cellular and molecular immunology. 8th ed. Philadelphia: Elsevier Saunders. 2015. p. 1-437.
  4. Williams IR, Kupper TS. Cytokines. In: Goldsmith LA, Katz SI, Gilchrest BA, Paller AS, Leffell DJ, Wolff. Fitzpatrick’s dermatology in general medicine. 8th ed. Volume 1. New York: McGraw-Hill. 2012. P. 126-41.
  5. Boskabady MH, Keyhanmanesh R, Khameneh S, Doostdaar Y, Khakzad MR. Potential immunomodulation effect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs. J Zhejiang Univ-Sci B (Biomed & Biotechnol). 2011;12(3):201-9
  6. Salem ML. Review: Immunomodulatory and therapeutic properties of the nigella sativa L. seed. International Immunopharmacology. 2005;5:1749-70.
  7. Majdalawieha AF, Hmaidana R, I R, Carrb. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. Journal of Ethnopharmacology. 2010;131(2):268-75.
  8. Ramadan MF. Nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa): an overview. Int J Food Sc and Tech. 2007;42:1208-18.
  9. Ali BH, Blunden G. Pharmacological and toxicological properties of nigella sativa. Phytother Res. 2003;17:299-305
  10. Gali-Muhtasib H, El-Najjar N, Stock RS. The medicinal potential of black seed (Nigella sativa) and its components. In: Khan MTH, Ather AA. Lead molecules from natural products: discovery and new trends. Elsevier B.V. 2006. P.133-53.
  11. Gholamnezhad Z, Boskabady MH, Hosseini M. Effects of Nigella sativa on immune response in treadmill exercised rat. Complementary and Alternative Medicine 2014;14:437-44
  12. Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine. 2002;9(1):69-74.
  13. Kamal El-Din Hussein El-Tahir, Bakeet DM. The Black Seed Nigella sativa Linnaeus - A Mine for Multi Cures: A Plea for Urgent Clinical Evaluation of its Volatile Oil Journal of Taibah University Medical Sciences. 2006;1(1):1.