DOI: https://doi.org/10.14710/jbtr.v2i1.331

The Effect of Extra Virgin Olive Oil on LOX-1 and COX-2 in High Fat Diet Rats

Faculty of Medicine Diponegoro University, Indonesia

Received: 25 Jan 2016; Published: 30 Jul 2016.
Open Access Copyright (c) 2016 Journal of Biomedicine and Translational Research

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Abstract

Background: High fat diet is a diet containing large amounts of fat consistently, the increase dietary fat and cholesterol which have a key role in growing health problems. Extra virgin olive oil associated with prevention of LDL oxidation, beneficial changes in lipid ratios and low risk for CHD.
Objective: to determine the anti-inflammatory effect  of extra virgin olive oil extract to  levels of COX-2 and LOX-1 in the blood in rats induced by high fat diet
Methods: This research is an experimental study that used randomized posttest only control group design. 30 Wistar rats which were divided into five groups:  group of control (-) which received  normal diet and group of control (+) which received  high fat diet without  EVOO treatment and three high fat diet groups treated by EVOO 1 mL/kg/day, 2 mL/kg/day and 3 mL/kg/day orally for 2 months. The blood was collected from eyes rats and serum separation by centrifuge. COX-2, LOX-1 concentration was measured by the enzyme linked immunosorbent assay (ELISA).
Results: The result of this study showed higher COX-2 concentrations in groups treated with EVOO than control group. COX-2 serum levels of negative control where significantly lower than those of rats treated with 2 ml/kg/day (p = 0.047) and 3 ml/kg/day EVOO (p = 0.014). The COX-2 serum levels of group received 1 ml/kg/day were significantly lower than those of rats received 3 ml/kg/day EVOO (p = 0.027). And showed  not significantly deferent among all groups (p = 0.570).
Conclusions: The conclusion of this study has showed that extra virgin olive oil extract might have minor anti inflammatory and antioxidant effect in rats.

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Keywords: EVOO; HFD; LOX-1; COX-2.

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Section: Original Research Articles
Language : EN
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  11. Belton O, Fitzgerald D, Dublin MD, Ireland.Cyclooxygenase-2 Inhibitors and Atherosclerosis. J Am CollCardiol. 2003; 41 (10):1820–2
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  14. Guasch-Ferré M, Hu F B, Martínez-González MA, et al. Olive oil intake and risk of cardiovascular disease and mortality in the predimed Study. BMC Medicine 2014, 12:78
  15. Moreno Jj. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free RadicBiol Med 2003; 35: 1073-81
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