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The Effect of Extra Virgin Olive Oil on LOX-1 and COX-2 in High Fat Diet Rats

Faculty of Medicine Diponegoro University, Indonesia

Received: 25 Jan 2016; Published: 30 Jul 2016.
Open Access Copyright (c) 2016 Journal of Biomedicine and Translational Research

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Abstract

Background: High fat diet is a diet containing large amounts of fat consistently, the increase dietary fat and cholesterol which have a key role in growing health problems. Extra virgin olive oil associated with prevention of LDL oxidation, beneficial changes in lipid ratios and low risk for CHD.
Objective: to determine the anti-inflammatory effect  of extra virgin olive oil extract to  levels of COX-2 and LOX-1 in the blood in rats induced by high fat diet
Methods: This research is an experimental study that used randomized posttest only control group design. 30 Wistar rats which were divided into five groups:  group of control (-) which received  normal diet and group of control (+) which received  high fat diet without  EVOO treatment and three high fat diet groups treated by EVOO 1 mL/kg/day, 2 mL/kg/day and 3 mL/kg/day orally for 2 months. The blood was collected from eyes rats and serum separation by centrifuge. COX-2, LOX-1 concentration was measured by the enzyme linked immunosorbent assay (ELISA).
Results: The result of this study showed higher COX-2 concentrations in groups treated with EVOO than control group. COX-2 serum levels of negative control where significantly lower than those of rats treated with 2 ml/kg/day (p = 0.047) and 3 ml/kg/day EVOO (p = 0.014). The COX-2 serum levels of group received 1 ml/kg/day were significantly lower than those of rats received 3 ml/kg/day EVOO (p = 0.027). And showed  not significantly deferent among all groups (p = 0.570).
Conclusions: The conclusion of this study has showed that extra virgin olive oil extract might have minor anti inflammatory and antioxidant effect in rats.

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Keywords: EVOO; HFD; LOX-1; COX-2.

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  1. According to the DRIs 2002 (updated 03/19/04) Available From: http://nutritionandaging.fiu.edu/DRI_and_DGs/DRI_and_RDAs.asp
  2. Mukherjee S, Mitra A. Health Effects of Palm Oil. J Hum Ecol, 2009; (3): 197-203
  3. Yusuf S, Ôunpuu S, Anand S. The Global Epidemic of Atherosclerotic Cardiovascular Disease. Med Principles Pract 2002;11( 2):3–8
  4. Mark A. Crowther. ? Pathogenesis of Atherosclerosis. American Society of Hematology 2005; 436-41
  5. Mehta J L, Li D, awahar. Identification, regulation and function of a novel lectin-like oxidized low-density lipoprotein receptor. J Am CollCardiol. 2002;39(9):1429-35
  6. Lusis AJ. Atherosclerosis. Nature 2000;407:233-41
  7. Mark A. Crowther.? Pathogenesis of Atherosclerosis.American Society of Hematology 2005; 436-41
  8. Turini M.E,DuBois R.N. Cyclooxygenase-2:A Therapeutic Target. Annu. Rev. Med. 2002;(53):35–57
  9. MAZHAR.D, GILLMORE. R, WAXMAN. J. COX and Cancer. Q J Med 2005; 98:711–18
  10. Ricciotti E, FitzGerald G.A. Prostaglandins and Inflammation. ArteriosclerThrombVasc Biol. 2011 May; 31(5): 986–1000
  11. Belton O, Fitzgerald D, Dublin MD, Ireland.Cyclooxygenase-2 Inhibitors and Atherosclerosis. J Am CollCardiol. 2003; 41 (10):1820–2
  12. Kim SY, Jun TW, Lee YS, Na HK, Surh YJ, Song W. Effects of exercise on cyclooxygenase-2 expression and nuclear factor-kappaB DNA binding in human peripheral blood mononuclear cells. Ann N Y Acad Sci. 2009 Aug;1171:464-71
  13. Covas MI, Nyyssonen K, Poulsen HE, et al. The effect of polyphenols in olive oil on heart disease risk factors: a randomized trial. Ann In tern Med 2006;145:333-41
  14. Guasch-Ferré M, Hu F B, Martínez-González MA, et al. Olive oil intake and risk of cardiovascular disease and mortality in the predimed Study. BMC Medicine 2014, 12:78
  15. Moreno Jj. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free RadicBiol Med 2003; 35: 1073-81
  16. Miles Ea, Zoubouli P, Calder Pc. Differential anti-inflammatory effects of phenolic compounds from extra virgin olive oil identified in human whole blood cultures. Nutrition 2005; 21: 389-94
  17. Keita H, Ramirez-San JuanE, Paniagua-CastroN, Garduno-SicilianoL, QuevedoL.The long-term ingestion of a diet high in extra virgin olive oil produces obesity and insulin resistance but protects endothelial function in rats: a preliminary study. DiabetolMetabSyndr 2013; 5: 53
  18. Susanti E, Rudijanto A, Ratnawati R. Catechins inhibit atherosclerosis in male rats on a high fat diet. Univ Med 2012; 81-7
  19. Campolo M, Paola RD,Impellizzeri D,et al. Effects of a polyphenol present in olive oil, oleuropeinaglycone, in a murine model of intestinal ischemia/reperfusion injury. J LB 2013; 277-87
  20. Morawietz H. LOX-1 and Atherosclerosis: Proof of Concept in LOX-1-Knockout Mice. Circ Res. 2007;100:1534-36
  21. Gleissner CA, Leitiniger N, Ley K. Effects of Native and Modified Low-Density Lipoproteins on Monocyte Recruitment in Atherosclerosis. Hypertension. 2007;50:1-8
  22. Covas M I, Nyysso K,Poulsen H E, et al.The Effect of Polyphenols in Olive Oil on Heart Disease Risk Factors. Ann Intern Med 2006; 145:333-41
  23. Kathryn C B, Sammy W M, Wong Y, Leng L, Bucala R. Soluble lectin-like oxidized low density lipoprotein receptor-1 in type 2 diabetes mellitus.J LR 2008;1438-44
  24. Hayashida K, Kume N, Murase T, Minami M,et al. Serum Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels Are Elevated in Acute Coronary Syndrome:ANovel Marker for Early Diagnosis.Circulation2005;112:812-18

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