DOI: https://doi.org/10.14710/jbtr.v12i1.28945

Biochemical and Histopathological Effect of Combination Extract Ethanolic Turmeric (Curcuma longa) and Kalmegh (Andrographis paniculata) in Iron Overload Rat Model

1Program Study of Nursing Science, Sekolah Tinggi Ilmu Kesehatan Permata Nusantara, Indonesia

2Department of Genetic and Molecular Medicine, Universitas Jenderal Soedirman, Indonesia

3Department of Environmental Toxicology, Universitas Jenderal Soedirman, Indonesia

Received: 10 Aug 2025; Revised: 25 Nov 2025; Accepted: 26 Jan 2026; Available online: 29 Apr 2026; Published: 30 Apr 2026.
Open Access Copyright (c) 2026 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Abstract

Background: Thalassemia-β patients have erythrocyte damage that requires blood transfusion treatment. Blood transfusion treatment that is undergone has an impact on the condition of iron overload in the body. The condition of iron overload will result in oxidative stress and organ damage that requires additional therapy

Objective: aimed to compare the effectiveness of turmeric and kalmegh extract doses against biochemical parameters and histopathological features of iron-induced liver Rattus Norvegicus.

Methods: This study used a True-experiment research design with a post-test-only control group. A total of 30 rats were used and divided into six treatment groups, normal, deferiprone drugs, turmeric, and kalmegh extracts 100mg/KgBW, 200mg/KgBW, and 400mg/KgBW. Malondialdehyde, catalase, superoxide dismutase, and ferritin are used as biochemical parameters and hepatic histopathology is documented.

Results: The results showed that there were significant improvements and differences in iron-induced rats.

Conclusion: The study also showed that turmeric and kalmegh extracts had similar effects to deferiprone.

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Keywords: Iron Overload; Kalmegh; Oxidative stress; Thalassemia; Turmeric
Funding: Thanks to BPPT (Balai Pembiayaan Pendidikan Tinggi) Beasiswa Pendidikan Indonesia, Kementerian Pendidikan Kebudayaan Riset dan Teknologi, Lembaga Pengelola Dana Pendidikan (LPDP)

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Section: Original Research Articles
Language : EN
  1. Krittayaphong R, Viprakasit V, Saiviroonporn P, et al. Blood Cells , Molecules and Diseases Prevalence and predictors of cardiac and liver iron overload in patients with thalassemia : A multicenter study based on real-world data. Blood Cells, Mol Dis 2017; 66: 24–30
  2. Ngim CF, Lee MY, Othman N, et al. Prevalence and Risk Factors for Cardiac and Liver Iron Overload in Adults with Thalassemia in Malaysia Prevalence and Risk Factors for Cardiac and Liver Iron Overload in Adults with. Hemoglobin 2019; 43: 95–100
  3. Obrzut M, Atamaniuk V, Glaser KJ, et al. Value of liver iron concentration in healthy volunteers assessed by MRI. Sci Rep 2020; 1–8
  4. Yassin MA, Soliman AT, De Sanctis V, et al. Statural growth and prevalence of endocrinopathies in relation to liver iron content (LIC) in adult patients with beta thalassemia major (BTM) and sickle cell disease (SCD). Acta Biomed 2018; 89: 33–40
  5. Kurtoğllu AU, Koçtekin B, Kurtoğlu E, et al. The effect of splenectomy on complement regulatory proteins in erythrocytes in b-thalassemia major. Arch Med Sci 2019; 15: 191–195
  6. Ayu DR, Sari DP. Skrining Thalassemia pada remaja di Desa Tanjung Pering Kecamatan Indralaya Ogan Ilir. J Pengabdi Masy Humanit Med 2020; 1: 125–130
  7. Goh LPW, Chong ETJ, Lee PC. Prevalence of alpha(α)-thalassemia in Southeast Asia (2010–2020): A meta-analysis involving 83,674 subjects. Int J Environ Res Public Health 2020; 17: 1–11
  8. Ali HM, Muhyi A, Riastiti Y. Hubungan Usia, Kadar Hemoglobin Pretransfusi dan Lama Sakit terhadap Kualitas Hidup Anak Talasemia di Samarinda. J Sains dan Kesehat 2021; 3: 441–447
  9. Susanto ZA, Siswandari W, Rujito L. Cd60 (GTG > GAG)/Hb Cagliari mutation was found in scanning of β-thalassemia alleles from patients of East Kalimantan, Indonesia. Mol Genet Metab Reports 2020; 22: 100550
  10. Harish, Pasha SJ. Correlation of Serum Ferritin Levels with Liver Function Tests and Anthropometric Measurements in Transfusion Dependent Beta-Thalassemia Major Children: A Cross Sectional Study. Pediatr Oncall 2019; 16: 101–104
  11. Zarghamian P, Azarkeivan A, Arabkhazaeli A, et al. Hepcidin gene polymorphisms and iron overload in β-thalassemia major patients refractory to iron chelating therapy. BMC Med Genet; 21. Epub ahead of print April 2020. DOI: 10.1186/s12881-020-01011-3
  12. Sahiratmadja E, Wijaya MA, Widjajakusuma A, et al. Pengetahuan Tentang Talasemia pada Mahasiswa Kedokteran dan Dokter Umum di Bandung serta Prevalensi Karir β-Thalassemia. J Indones Med Assoc 2020; 70: 48–58
  13. Czaja AJ. Review article: iron disturbances in chronic liver diseases other than haemochromatosis – pathogenic, prognostic, and therapeutic implications. Aliment Pharmacol Ther 2019; 49: 681–701
  14. Eghbali A, Khalilpour A, Taherahmadi H, et al. Pantoprazole reduces serum ferritin in patients with thalassemia major and intermedia: A randomized, controlled study. Therapie 2019; 74: 507–512
  15. Patpan N, Banjerdpongchai R, Tantiworawit A, et al. The Effect of Transfusion-Dependent Thalassemia Patient’s Serum on Peripheral Blood Mononuclear Cell Viability. J Cell Death 2019; 12: 1–8
  16. Mufid A, Oktarlina RZ. Efektivitas Nigella Sativa sebagai Terapi Adjuvan pada Pasien Beta Thalasemia. Majority 2019; 8: 195–201
  17. Adiyasa MR, Meiyanti M. Pemanfaatan obat tradisional di Indonesia: distribusi dan faktor demografis yang berpengaruh. J Biomedika dan Kesehat 2021; 4: 130–138
  18. Umapathy VR, Swamikannu B, Jones S, et al. Effects of tumerric (Curcuma longa) on Oral Health. Biomed Informatics 2022; 18: 538–542
  19. Owoade AO, Alausa AO, Adetutu A, et al. Protective effects of methanolic extract of Andrographis paniculata (Burm.f.) Nees leaves against arsenic-induced damage in rats. Bull Natl Res Cent 2022; 46: 1–11
  20. Rigoli L, Petrungaro A, Di Bella C, et al. Iron overload and malignancies in patients with haemoglobinopathies: A single center experience. Transfus Apher Sci 2019; 58: 647–651
  21. Daru J, Colman K, Stanworth SJ, et al. Serum ferritin as an indicator of iron status: What do we need to know? Am J Clin Nutr 2017; 106: 1634S-1639S
  22. Rainey NE, Moustapha A, Saric A, et al. Iron chelation by curcumin suppresses both curcumin-induced autophagy and cell death together with iron overload neoplastic transformation. Cell Death Discov; 5. Epub ahead of print 2019. DOI: 10.1038/s41420-019-0234-y
  23. Bhargavi B, Kalpana K, Kistamma S. Analysis of minerals under different water stress levels in Andrographis paniculata (Burm.f.) Wall. Ex Nees. J Drug Deliv Ther 2019; 9: 48–50
  24. Priyadarsini KI. The chemistry of curcumin: From extraction to therapeutic agent. Molecules 2014; 19: 20091–20112
  25. Wang X, Li Y, Han L, et al. Role of Flavonoids in the Treatment of Iron Overload. Front Cell Dev Biol 2021; 9: 1–12
  26. Takea, Mhsen SN. Turmeric Oil Effect on the Reproductive Efficacy of Female Albino Rats Treated with Iron Overload. Tikrit J Agric Sci 2018; 18: 124–130
  27. Hidayat R, Wulandari P. Effects of Andrographis paniculata (Burm. F.) Extract on Diabetic Nephropathy in Rats. Reports Biochem Mol Biol 2021; 10: 445–454
  28. Francis P, Navarro VJ. Drug-Induced Hepatotoxicity. In: NCBI Book. 2023, pp. 6–11

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