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The Effect of Eurycoma longifolia Jack on sICAM-1 and eNOS in Rats with High Fat Diet

Faculty of Medicine Diponegoro University, Indonesia

Received: 17 May 2018; Published: 31 Jul 2018.
Open Access Copyright (c) 2018 Journal of Biomedicine and Translational Research

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Background: High fat diets are known to cause a positive fat balance and consequently to the accumulation of adipose mass, this diet does not seem to stimulate fat oxidation in the same way in obese and lean subjects. HFD was an inducing factor for ICAM-1 expression in the aorta of Wistar rats. HFD effect on ICAM-1 seems to be time dependent. ICAM-1 is one of the first events in the formation of atherosclerotic lesions. HFD up-regulated Cav-1, regulated expression other biomarker in HFD is eNOS. Recent studies showed that E. longifolia Jack protected HFD animal model from atherosclerosis based on the reduce atherosclerotic plaque size and formation HFD-rats treated with E. longifolia Jack.

Objective: To prove that Eurycoma longifolia has anti inflammatory effect on endothelial cell blood vessels of Sprague Dawley rat with high fat diet.

Method: Study design was experimental study, by used Randomized Post Test only Control Group Design with Kruskal-Wallis test was used to analyze the differences among groups and followed by a Mann Whitney test. Treatment is ethanolic or water extract of Eurycoma longifolia Jack, and out come are sICAM-1 and eNOS levels. Thirty Sprague Dawley (SD) Rat, were divided into 6 groups. C(-) was SD group, C(+) was group with HFD, X1 (SD treated with EL dosage 10 mg/kg), X2 (SD treated with EL dosage 15 mg/kg), X3 (HFD treated with EL dosage 10 mg/kg), X4 (HFD treated with EL dosage 15 mg/kg).

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Keywords: Eurycoma longifolia Jack; sICAM; eNOS; HFD.

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  1. D.F. Coelho, L.O. Pereira-Lancha, D.S. Chaves, D. Diwan, R. Ferraz, P.L. Campos-Ferraz, J. R. Poortmans and A.H. Lancha Junior. Effect of high-fat diets on body composition, lipid metabolism and insulin sensitivity, and the role of exercise on these parameters. Braz J. Med Biol Res. 2011; 44(10): 966-72
  2. Matsuzawa-Nagata N, Takamura T, Ando H, Nakamura S, Kurita S, Misu H, Ota T, et. al. Increased oxidative stress precedes the onset of high-fat diet-induced insulin resistance and obesity.Metabolism. 2008; 57(8):1071-7
  3. Mai A, Jian-Mei Li. NADPH Oxidase Activation And Oxidative Stress In High-Fat Diet-Induced Hypertension And Metabolic Disorders. Heart 2014; 100: A1
  4. A. Barnes. Overweight versus obese: different risk and different management. Tex Heart Inst J. 2015; 42 (3): 237–238
  5. World Health Organization. Global Health Observatory (GHO) data. Overweight and obesity World Health Organization, Geneva 2014
  6. J.W. Yun. Possible anti-obesity therapeutics from nature-a review. Phytochemistry 2010; 71: 1625–1641
  7. K.A. Martin, M.V. Mani, A. Mani. New targets to treat obesity and the metabolic syndrome. Eur J Pharmacol. 2015
  8. LamprosFotis, GeorgiosAgrogiannis, Ioannis S. Vlachos, AlkistisPantopoulou, AngelikiMargoni, Maria Kostaki, et. al. Intercellular Adhesion Molecule (ICAM)-1 and Vascular Cell Adhesion Molecule (VCAM)-1 at the Early Stages of Atherosclerosis in a Rat Model. In Vivo. 2012. 26: 243-250
  9. NianhongYang, ChenjiangYing, MingjiaXu, XuezhiZuo, XiaoleiYe, LiegangLiu, et. al. High-fat diet up-regulates caveolin-1 expression in aorta of diet-induced obese but not in diet-resistant rats. Cardiovascular Research 2007; 76: 167–174
  10. Daniel A. Dias, Sylvia Urban, and Ute Roessner. A Historical overview of natural products in drug discovery. Metabolites 2012; 2: 303-336
  11. Kuo PC, Damu AG, Lee KK and Wu TS. Cytotoxic and Antimalarial Constituent from The Roots of Eurycomalongifolia. J Bioorg Med Chem. 2004; 12: 537 -544
  12. Guo Z, Vangapandu S, Sindelar RW, Walker LA and Sindelar RD. Biologically Active Quassinoid and Their Chemistry: Potensial Leads for Drug Design. Curr Med Chem. 2005; 12:190-193
  13. Kuo PC, Shi LS, Damu AG, Su CR, Huang CH, Ke CH, Wu JB, et. al. Cytotoxic and Antimalarial Beta-carboline Alkaloids from The Roots of Eurycomalongifolia. J Nat Prod. 2003; 66(10): 1324–1327
  14. Fakhria Al-Joufi, Imad M. Al-Ani, Anil K. Saxena, Norlelawati A. Talib, Rafidah H. Mokhtar, Norsidah Ku-Zaifah. Assessment of anti-atherosclerotic effect of Eurycomalongifolia extract on high fat diet model in rats. I: Histological study. Eur. J. Anat. 2016; 20(2): 131-136
  15. Fakhria Al-Joufi, Anil K. Saxena, Imad M. Al-Ani, Norlelawati A. Talib, Rafidah H. Mokhtar, Norsidah Ku-Zaifah. Anti-Atherosclerotic Effects of Eurycomalongifolia in Rats Fed on High Fat Diet. IMJM. 2015: 14

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