DOI: https://doi.org/10.14710/jbtr.v1i1.13440

The Discontinuation Effect of Topical Prednisolone on Extracellular Matrix Trabecular Meshwork in Wistar Rat

1Faculty of Medicine, Universitas Diponegoro, Indonesia

2Dr Kariadi General Hospital, Indonesia

Received: 17 Jan 2022; Revised: 27 Apr 2022; Accepted: 27 Apr 2022; Available online: 28 Apr 2022; Published: 28 Apr 2022.
Open Access Copyright (c) 2022 Journal of Biomedicine and Translational Research
Creative Commons License This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

ABSTRACT 

Background:Intra ocular pressure (IOP) elevationis one of topical steroids’ side effects. Corticosteroid will initiate matrix metalloproteinase cascade that leads to extra cellular matrix (ECM)of trabecular meshwork (TM) turnover(remodeling) and its acumulationin TM. 

Objective: To analyze the reversibility of ECM TM in Wistar rat after discontinuation oftopical prednisolone1% at different periode(14 days and 28 days). 

Methods:This was an experimental study with post test only controlgroup design. Total samples of 28 rats were divided into 4 groups: Treatment groups 1 was treated with  topical prednisolone for 28 days, and was terminated 14 days after discontinuation of the drug.Treatment goups2 was treated topical prednisolone for 28 days and was terminated28 days after discontinuation of the drug. Control 1 was treated withtopical prednisolone for 28 days, and Control 2 was treated with topical saline for 28 days and terminated without periode of discontinuation. Histopathological grading score was used to evaluate ECM TM deposition. Mann-Whitney test and Kruskal-Wallis test were used to analyze the data. 

Result: Deposition of ECM in TM was not statistically different intreamentgroup 1 and treatment group 2 (p>0.05). Deposition of ECM in TM were statistically differentbetween treatment group 2 and control group 1 (p<0.05). Comparative test showed p<0.001,which means that there was a change in the  thickness of ECM after discontinuation of instillation. 

Conclusion: ECM TM was thinner in the experimental animals with a longer duration of topical prednisolondiscontinuation, which demonstrate that maintenance remodeling of ECM was happen.

Fulltext View|Download
Keywords: Prednisolone; extracellular matrix; trabecular meshwork

Article Metrics:

Article Info
Section: Original Research Articles
Language : EN
Recent articles
The role of Zinc Intake in Serotonin and Cortisol Level in Patient with Depression Diagnostic value of SARS-CoV-2 RDT-Ab with RT-PCR: Secondary data at Diponegoro National Hospital Dilemma of presymptomatic testing in children with history of late onset neurodegenerative spinocerebellar ataxia in Indonesia The effect of fibre intervention on serum and faecal short-chain fatty acids in human with overweight or obesity: a systematic review of human intervention studies Effect of Combination Songga-Wood-Stem (Strychnos ligustrina Blume) and Antimalaria-ACT on IL-10 Production of Malaria More recent articles
  1. Quigley HA, Broman AT. The Number of People with Glaucoma Worldwide in 2010 and 2020. Br. J. Ophthalmol. 2006; 90(3): 262-267. doi: 10.1136/bjo.2005.081224
  2. Chan EW, Li X, Tham YC, Liao J, Wong TY, Aung T et al. Glaucoma in Asia: Regional Prevalence Variations and Future Projections. Br J Ophthalmol. 2016 Jan; 100(1): 78-85. doi: 10.1136/bjophthalmol-2014-306102
  3. Tham YC, Xiang L, Tien YW, Quigley HA, Cheng CY. Global Prevalence of Glaucoma and Projections of Glaukoma Burden Through 2040. AAO Journal. Ophthalmology. 2014 Nov; 121(11): 2081-90. doi: 10.1016/j.ophtha.2014.05.013
  4. Kementerian Kesehatan RI. Pusat Data dan Informasi. Situasi dan Analisis Glaukoma. 2015; 2. Retrieved from https://pusdatin.kemkes.go.id/article/view/19080500002/situasi-glaukoma-di-indonesia.html
  5. Cheng JW, Ying Zong, You-Yan Zheng, Rui-Li Wei. The Prevalence of Primary Angle Closure Glaucoma in Adult Asians: A Systematic Review and Meta-Analysis. PLoS ONE 9(7): e103222. https://doi.org/10.1371/journal.pone.0103222
  6. Sheppard JD, TL Comstock, ME Carvet. Impact of the Topical Ophthalmic Cortiocosteroid Loteprednol Etabonate in Intraocular Pressure. Adv. Ther. 2016; 33(4): 532-52. doi: 10.1007/s12325-016-0315-8
  7. Guorong Li, Gang Cui., WM Dismuke, I Navarro, K Perkumas, DF Woodward, WD Slamer. Differential Respons and Withdrawal Profile of Glucocorticoid-treated Human Trabecular Meshwork Cell. Experimental Eye Research. 2017; 155:38-46. doi: 10.1007/s12325-016-0315-8
  8. Overby R.D. and Abbot F.C. Animal Model od Glucocorticoid-Induced Glaucoma. Exp. Eye Res. 2016; 141: 15-22. doi: 10.1016/j.exer.2015.06.002
  9. Raghunathan VK, Jukia B, Ramesh K, Zode G , Solemi M, Phinney BS et al . Glaucomatous Cell Derived Matrices Differently Modulate Non-Glaucomatous Trabecular Meshwork Cellular Behavior. 2018; 15; 71; 444-459. doi: 10.1016/j.actbio.2018.02.037
  10. Vranka A, Janice KM, Mary JK, Ted A, Keller KE. Extracellular Matrix in the Trabecular Meshwork: Intraocular Pressure Regulation and Dysregulation in Glaucoma. Exp. Eye Res. 2015; 133: 112-125. doi: 10.1016/j.exer.2014.07.014
  11. Reinehr S. Complement Activation and Extracellular Matrix Remodeling in an Autoimmune Glaucoma Model [Dissertation]. German: Faculty of Biology and Biotechnology Ruhr-University Bochum. 2016. Retrieved from https://hss-opus.ub.ruhr-uni-bochum.de/opus4/frontdoor/deliver/index/docId/5093/file/diss.pdf
  12. Tawara A., Norihiko T, Tshiaka K, Y Harada, K Yakota. Immunohistochemical Evaluation of the Extracellular Matrix in Trabecular Meshwork in Steroid Induced Glaucoma. Clin. Exp. Ophthalmol. 2008; 246; 1021-1028. doi: 10.1007/s00417-008-0800-0
  13. Becker B, RN Shaffer. Diagnosis and Therapy of Glaucoma. 8th edition. In: Robert L Stampler, Marc FL, and Michael VD, editors. Aqueous Humor Outflow System Overview. United State: Elsevier; 2009. p. 25-42
  14. Rhee DJ, Ramez IH, Min HL, and Dong-Jin O. Matricellular Proteins in The Trabecular Meshwork. Exp Eye Res. 2009 Apr; 88(4):694-703. doi: 10.1016/j.exer.2008.11.032
  15. Li B, Erica P, David CC. Prednisolone Induces Time- And Dose-dependent Ocular Hypertension In Rats. Invest. Ophthalmol. Vis. Sci.2012;53(14):1970
  16. Tektas OY, Christian MH, John D. Morphologic Changes in the Outflow Pathways of Bovine Eyes Treated with Corticosteroids. Invest Ophthalmol Vis Sci. 2010 Aug; 51(8): 4060–4066. doi: 10.1167/iovs.09-4742
  17. Tamm ER, Barbara MB, Rudolf F. Intraocular Pressure and the Mechanisms Involved in Resistance of the Aqueous Humor Flow in the Trabecular Meshwork Outflow Pathways. Prog Mol Biol Transl Sci. 2015;134:301-14. doi: 10.1016/bs.pmbts.2015.06.007
  18. Genis A. The Effects of Glucocorticoids on Trabecular Meshwork and its Role in Glaucoma. American J. of Biochemistry and Biotechnology. 2015; Vol.11; Issue 4; 185-190. https://doi.org/10.3844/ajbbsp.2015.185.190
  19. Junglas B., Alice HLY, Ulrich WL, Ernst RT, Rudolf F. Connective Tissue Growth Factor Induces Extracellular Matrix Deposition in Human Trabecular Meshwork Cells. Exp Eye Res. 2009 Jun;88(6):1065-75. doi: 10.1016/j.exer.2009.01.008
  20. Groef LD, Inge VH, Eline D, Ingeborg S, Mieve M. MMPs in the Trabecular Meshwork: Promising Targets for Future Glaucoma Therapies?. Investigative Ophthalmology & Visual Science November 2013, Vol.54, 7756-7763. doi: https://doi.org/10.1167/iovs.13-13088

Last update:

No citation recorded.

Last update:

No citation recorded.