DOI: https://doi.org/10.14710/gjec.2023.21369

Analisis Aktivitas Antiglikasi pada Berbagai Konsentrasi Ekstrak Etanol dari Produk Fermentasi Daun Matoa

Adhina Choiri Putri  -  Department of Chemistry, Diponegoro University, Indonesia
Meiny Suzery  -  Department of Chemistry, Diponegoro University, Indonesia
*Agustin L.N. Aminin orcid scopus  -  Department of Chemistry, Diponegoro University, Indonesia
Open Access Copyright 2024 Greensphere: Journal of Environmental Chemistry

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Abstract

Penyebab penyakit degeneratif salah satunya disebabkan oleh kondisi penurunan fungsi organ dalam tubuh yang dapat ditandai dari kerusakan struktur protein. Reaksi glikasi protein yang menghasilkan produk amadori mengarah pada pembentukan AGEs (Advanced Glycation End Products) yang bersifat radikal. Senyawa antiglikasi memiliki potensi menghambat kerusakan protein lebih lanjut. Metode solid state fermentation (SSF) menggunakan Aspergillus niger dapat menghasilkan enzim yang melimpah, yang berkontribusi terhadap degradasi makromolekul dan mampu meningkatkan bioaktivitasnya. Penelitian ini berfokus pada penentuan aktivitas antiglikasi produk fermentasi daun matoa. Variasi konsentrasi pada pengujian aktivitas antiglikasi dilakukan pada konsentrasi 50 hingga 1000 ppm. Data menunjukkan persen inhibisi produk fermentasi lebih rendah dibanding produk non-fermentasi. Semakin tinggi konsentrasi yang digunakan maka semakin tinggi persen inhibisinya. Variasi konsentrasi menunjukkan adanya pengaruh yang signifikan terhadap penurunan aktivitas antiglikasi (p<0,05). Aktivitas antiglikasi daun matoa sebelum dan sesudah fermentasi masuk dalam kategori sedang (medium).

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Keywords: variasi konsentrasi, fermentasi solid state; Aspergillus niger; antiglikasi
Funding: Universitas Diponegoro

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  1. A. Dalimunthe, P. A. Z. Hasibuan, J. Silalahi, and D. Satria, “Antioxidant activity of alkaloid fractions of litsea cubeba lour. Fruits,” Asian J. Pharm. Clin. Res., vol. 11, no. Special Issue 1, pp. 31–32, 2018, doi: 10.22159/ajpcr.2018.v11s1.26558
  2. E. Reynaud, “Protein misfolding and degenerative diseases,” Nat. Educ., vol. 3, no. 9, p. 28, 2010
  3. A. A. Chinchansure, A. M. Korwar, M. J. Kulkarni, and S. P. Joshi, “Recent development of plant products with anti-glycation activity: A review,” R. Soc. Chem., vol. 3, 2015, doi: 10.1039/C4RA14211J
  4. A. B. Lindner and A. Demarez, “Protein aggregation as a paradigm of aging,” Biochim. Biophys. Acta - Gen. Subj., vol. 1790, no. 10, pp. 980–996, 2009, doi: 10.1016/j.bbagen.2009.06.005
  5. G. Abbas, A. S. Al-Harrasi, H. Hussain, J. Hussain, R. Rashid, and M. I. Choudhary, “Antiglycation therapy: Discovery of promising antiglycation agents for the management of diabetic complications,” Pharm. Biol., vol. 54, no. 2, pp. 198–206, 2016, doi: 10.3109/13880209.2015.1028080
  6. K. Jiménez-Aliaga, P. Bermejo-Bescós, J. Benedí, and S. Martín-Aragón, “Quercetin and rutin exhibit antiamyloidogenic and fibril-disaggregating effects in vitro and potent antioxidant activity in APPswe cells,” Life Sci., vol. 89, no. 25–26, pp. 939–945, 2011, doi: 10.1016/j.lfs.2011.09.023
  7. A. Precupas, R. Sandu, A. V. F. Neculae, A. Neacsu, and V. T. Popa, “Calorimetric, spectroscopic and computational investigation of morin binding effect on bovine serum albumin stability,” J. Mol. Liq., vol. 333, p. 115953, 2021, doi: 10.1016/j.molliq.2021.115953
  8. BPTP, “Buku Seri Tanaman Khas Papua : Matoa,” in Badan Pengkajian Teknologi Pertanian Papua, 2014
  9. D. Islami, L. Anggraini, and I. Wardaniati, “Aktivitas Antioksidan dan Skrining Fitokimia dari Ekstrak Daun Matoa Pometia pinnata,” J. Farm. Higea, vol. 13, no. 1, 2021
  10. N. W. Martiningsih et al., “Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol daun Matoa (Pometia pinnata) dengan metode DPPH,” Pros. Semin. Nas. MIPA, vol. 3, no. 3, pp. 332–338, 2016
  11. N. Elisa, F. Xaverius, and S. Wibowo, “Hypertension Profile of Angiotensin Receptor Blocker From Matoa Leaves Extract ( Pometia Pinnata J . R . Foster & G . Foster ) In Angiotensin II Induced-Male Rat,” Str. J. Ilm. Kesehat., vol. 9, no. 2, pp. 1830–1836, 2020, doi: 10.30994/sjik.v9i2.595
  12. L. Wulandari, A. S. Nugraha, and U. A. Himmah, “Aktivitas, Penentuan Ekstrak, Antidiabetes Matoa, Daun G Forst, Pometia J R Forst, IN Vitro,” J. Kefarmasian Indones., vol. 11, no. 2, pp. 132–141, 2021
  13. Nur Isra; Berna Elya; Sofrida Rosita Hanum ; Abdul Mun`im; Fadlina Chany Saputri, “Uji Aktivitas Penghambatan α-Glukosidase, Penetapan Kadar Fenol Total dan Flavonoid Total pada Ekstrak Daun dan Kulit Batang Matoa (Pometia pinnata) …,” Univ. Indones. Depok, 2018
  14. Adrian, R. A. Syahputra, S. Lie, S. Theo, and S. E. Nugraha, “Antioxidant, Total Phenol, Total Flavonoid, and LC-MS/MS Analysis of Pometia Pinnata Ethanol Extract,” InHeNce 2021 - 2021 IEEE Int. Conf. Heal. Instrum. Meas. Nat. Sci., p. 9537214, 2021, doi: 10.1109/InHeNce52833.2021.9537214
  15. Y. Cui et al., “Solid-state fermentation by Aspergillus niger and Trichoderma koningii improves the quality of tea dregs for use as feed additives,” PLoS One, vol. 16, no. 11 November, pp. 1–20, 2021, doi: 10.1371/journal.pone.0260045
  16. I. A. Parshikov and J. B. Sutherland, “The use of Aspergillus niger cultures for biotransformation of terpenoids,” Process Biochem., vol. 49, no. 12, pp. 2086–2100, 2014, doi: 10.1016/j.procbio.2014.09.005
  17. D. W. Harjanti, F. Wahyono, and V. R. Ciptaningtyas, “Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria,” 2020, doi: 10.14202/vetworld.2020.1187-1192
  18. Y. L. Wen, L. P. Yan, and C. S. Chen, “Effects of fermentation treatment on antioxidant and antimicrobial activities of four common Chinese herbal medicinal residues by Aspergillus oryzae,” J. Food Drug Anal., vol. 21, no. 2, pp. 219–226, 2013, doi: 10.1016/j.jfda.2013.05.013
  19. Ratnasari, B. Cahyono, M. Suzery, and A. L. N. Aminin, “Growth profile of Aspergillus niger on red galangal rhizomes as shown by bioactive compound changes,” IOP Conf. Ser. Mater. Sci. Eng., vol. 509, no. 1, 2019, doi: 10.1088/1757-899X/509/1/012118
  20. M. Starowicz and H. Zieliński, “Inhibition of advanced glycation end-product formation by high antioxidant-leveled spices commonly used in European cuisine,” Antioxidants, vol. 8, no. 4, 2019, doi: 10.3390/antiox8040100
  21. D. H. Truong, D. H. Nguyen, N. T. A. Ta, A. V. Bui, T. H. Do, and H. C. Nguyen, “Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of severinia buxifolia,” J. Food Qual., vol. 2019, 2019, doi: 10.1155/2019/8178294
  22. A. Aminah, N. Tomayahu, and Z. Abidin, “PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL KULIT BUAH ALPUKAT (Persea americana Mill.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS,” J. Fitofarmaka Indones., vol. 4, no. 2, pp. 226–230, 2017, doi: 10.33096/jffi.v4i2.265
  23. N. A. Yazid, R. Barrena, D. Komilis, and A. Sánchez, “Solid-state fermentation as a novel paradigm for organic waste valorization: A review,” Sustain., vol. 9, no. 2, pp. 1–28, 2017, doi: 10.3390/su9020224
  24. A. I. El-Batal and H. Abdel Karem, “Phytase production and phytic acid reduction in rapeseed meal by Aspergillus niger during solid state fermentation,” Food Res. Int., vol. 34, no. 8, pp. 715–720, 2001, doi: 10.1016/S0963-9969(01)00093-X
  25. P. J. Fellows, “Food processing technology: Principles and practice: Third edition,” Food Process. Technol. Princ. Pract. Third Ed., pp. 1–913, 2009, doi: 10.1533/9781845696344
  26. Ihtifazhuddin, M. Ikhwan, Nursyam, Happy, and A. W. Ekawati, “The Influence of Fermentation Time in the Physical and Chemical Composition of Fermented Soybean Husk by Using Aspergillus niger on the Quality of Raw Feed Materials,” J. Exp. Life Sci., vol. 6, no. 1, pp. 52–57, 2016, doi: 10.21776/ub.jels.2016.006.01.12
  27. D. S. Rejeki, A. L. N. Aminin, and M. Suzery, “Preliminary Study of Hyptis pectinata (L.) Poit Extract Biotransformation by Aspergillus niger,” IOP Conf. Ser. Mater. Sci. Eng., vol. 349, no. 1, 2018, doi: 10.1088/1757-899X/349/1/012004
  28. R. Kaprasob, O. Kerdchoechuen, N. Laohakunjit, D. Sarkar, and K. Shetty, “Fermentation-based biotransformation of bioactive phenolics and volatile compounds from cashew apple juice by select lactic acid bacteria,” Process Biochem., vol. 59, no. April, pp. 141–149, 2017, doi: 10.1016/j.procbio.2017.05.019
  29. S. A. Prayitno and A. R. Rahim, “The Comparison of Extracts (Ethanol And Aquos Solvents) Muntingia calabura Leaves on Total Phenol, Flavonid And Antioxidant (Ic50) Properties,” Kontribusia (Research Dissem. Community Dev., vol. 3, no. 2, p. 319, 2020, doi: 10.30587/kontribusia.v3i2.1451
  30. M. G. Rasul, “Conventional Extraction Methods Use in Medicinal Plants, their Advantages and Disadvantages,” Int. J. Basic Sci. Appl. Comput., no. 6, pp. 10–14, 2018
  31. E. Suryanto and M. Taroreh, “Aktivitas Antioksidatif Dan Anti-Glikasi Ekstrak Fenolik Bebas Dan Fenolik Terikat Dari Tongkol Jagung,” Chem. Prog., vol. 13, no. 2, 2020, doi: 10.35799/cp.13.2.2020.31393
  32. F. Asgharpour Dil, Z. Ranjkesh, and M. T. Goodarzi, “A systematic review of antiglycation medicinal plants,” Diabetes Metab. Syndr. Clin. Res. Rev., vol. 13, no. 2, pp. 1225–1229, 2019, doi: 10.1016/j.dsx.2019.01.053
  33. R. Zhao et al., “Measurement of amyloid formation by turbidity assay—seeing through the cloud,” Biophys. Rev., vol. 8, no. 4, pp. 445–471, 2016, doi: 10.1007/s12551-016-0233-7
  34. D. Fitriyani and R. Fatahillah, “Anti-Inflammatory Activity Of Ethanol Extract And Ethyl Acetate Fraction Of Kebiul (Caesalpinia bonduc L.) Seed Coat Against Inhibition Of Protein Denaturation,” J. Kim. Ris., vol. 7, no. 1, pp. 1–8, 2022, doi: 10.20473/jkr.v7i1.31108

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