DOI: https://doi.org/10.14710/gjec.2022.14794

Bakteri Endofit F4 dari Daun Pepaya (Carica papaya L): Potensinya sebagai Penghasil Enzim Ekstraseluler

*Purbowatiningrum Ria Sarjono  -  Chemistry Department, Diponegoro University, Indonesia
Ismiyarto Ismiyarto  -  Chemistry Department, Diponegoro University, Indonesia
Ngadiwiyana ngadiwiyana  -  Chemistry Department, Diponegoro University, Indonesia
Nor Basid Adiwibawa Prasetya  -  Chemistry Department, Diponegoro University, Indonesia
Open Access Copyright 2022 Greensphere: Journal of Environmental Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Citation Format:
Abstract

Abstract

Papaya leaves are known to have antibacterial activity, so the endophytic bacteria on the papaya leaves are thought to have antibacterial activity. Previous studies have obtained 5 isolates of endophytic bacteria from papaya leaves, including F1, F2, F3, F4 and F5. Endophytic bacteria F1, F3, and F5 have known antibacterial activity, while endophytic bacteria F4 have not yet explored their activity in producing extracellular enzymes, so that in this study a qualitative test of extracellular enzymes was carried out on endophytic bacteria F4. The purpose of this study was to obtain confirmation of morphology, qualitative phytochemical data, and the ability to produce extracellular enzymes lipase, cellulase, protease and amylase from endophytic bacteria F4.

The results obtained were F4 bacterial isolates white, round and smooth rounded edges which were gram positive with single cell morphology and the shape is bacillus (stems). F4 bacteria have the potential to contain amylase and protease extracellular enzymes.

Fulltext View|Download
Keywords: endophytic bacteria; extracellular enzymes; papaya leaf
Funding: Universitas Diponegoro

Article Metrics:

Article Info
Section: Articles
Language : ID
Recent articles
Potensi Antioksidan Ekstrak Etanol Residu Destilasi Sereh Wangi (Cymbopogon nardus) Enkapsulasi Vitamin C Dalam Silika dengan Prekursor TEOS/MTES/PVA melalui Proses Sol-Gel Sintesis Elektroda Lapis Tipis Sn–Zno/FTO Secara Elektrodeposisi dan Aplikasi pada Degradasi Remazol Black B (RBB More recent articles
  1. Yogiraj, Vijay, Pradeep Kumar Goyal, Chetan Singh Chauhan, Anju Goyal, Bhupendra Vyas, Carica papaya Linn: an overview, International Journal of Herbal Medicine, 2, 5, (2014), 01-08
  2. Ekaiko, U.M., Chiwendu Stephen, E.O. Ukpabi, Ezikpe C.A., Antimicrobial Screening and Phytochemical Analysis of Carica papaya Leaf Extracts, Standard Research Journal of Microbiological Sciences, 2, (2015), 1-4
  3. Doley, Prabhali, Dhruva Kumar Jha, Antimicrobial activity of bacterial endophytes from medicinal endemic plant Garcinia lancifolia Roxb, Annals of Plant Sciences, 4, 12, (2016), 1243-1247
  5. Dasa, Ipsita, Mrunmaya Kumar Pandaa, Chandi C. Rathb, Kumananda Tayungc, Bioactivities of bacterial endophytes isolated from leaf tissues of Hyptis suaveolens against some clinically significant pathogens, Journal of Applied Pharmaceutical Science, 7, 8, (2017), 131-136 http://dx.doi.org/10.7324/JAPS.2017.70818
  6. Nursulistyarini, Fenni, Erny Qurotul Ainy, Isolasi dan identifikasi bakteri endofit penghasil antibakteri dari daun tanaman Binahong (Anredera cordifolia (Ten.) Steenis), Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning, 2014
  7. Ramadhan, F.A., Isolasi Bakteri Endofit asal Daun Pepaya (Carica Pepaya) dan Uji Aktivitas Antibakteri dari Metabolit Sekundernya, Jurusan Kimia Universitas Diponegoro Semarang, 2015
  8. Sarjono, Purbowatiningrum Ria, Hendra Dwipa Rifky Mahardika, Nies S. Mulyani, Ngadiwiyana Ngadiwiyana, Nor Basid Adi Prasetyawibowo, Ismiyarto Ismiyarto, Aktivitas antidiabetes metabolit sekunder bakteri endofit asal kulit kayu manis, (2021),
  9. Sianturi, Dessy Christina, Isolasi bakteri dan uji aktivitas amilase termofil kasar dari sumber air panas Penen Sibirubiru Sumatera Utara, Ilmu Biologi, Universitas Sumatera Utara, Medan, 2008
  11. Charlena, Charlena, Profil kelarutan limbah minyak bumi dalam air akibat pengaruh surfaktan nonionik dan laju pengadukan, CHEMISTRY PROGRESS, 2, 2, (2019), 69-78
  12. Brooks, G. F., J. S. Butel, L. N. Ornston, E. Jawetz, J. L. Melnick, E. A. Adelberg, Medical parasitology, in: T.G. Mitchell (Ed.) Jawetz, Melnick, & Adelberg’s Medical Microbiology, McGraw-Hill, United Kingdom, 2004,
  14. Winarno, F.G., Enzim Pangan dan Gizi, Gramedia Pustaka Utama, Jakarta, 1986,
  15. Poedjiadi, Anna, F.M. Titin Supriyanti, Dasar-dasar Biokimia Edisi Revisi, UI-Press. Jakarta, (2006),
  17. Beng, Jeremy Mark, John L. Tymoczko, Lubert Stryer, Biochemistry, 7th ed., W.H. Freeman, Basingstoke, 2012,
  18. Tam, Jorge Juan, John R. Whitaker, Rates and extents of hydrolysis of several caseins by pepsin, rennin, Endothia parasitica protease and Mucor pusillus protease, Journal of Dairy Science, 55, 11, (1972), 1523-1531 https://doi.org/10.3168/jds.S0022-0302(72)85714-X
  19. Rutten, Rita, Andrew J. Daugulis, Continuous production of α-amylase byBacillus amyloliquefaciens in a two-stage fermentor, Biotechnology letters, 9, 7, (1987), 505-510 https://doi.org/10.1007/BF01027461
  21. Bajpai, Pratima, Pramod K. Bajpai, High‐temperature alkaline α‐amylase from Bacillus iicheniformis TCRDC‐B13, Biotechnology and Bioengineering, 33, 1, (1989), 72-78 https://doi.org/10.1002/bit.260330110
  22. Lowry, Oliver H., Nira J. Rosebrough, A. Lewis Farr, Rose J. Randall, Protein measurement with the Folin phenol reagent, Journal of Biological Chemistry, 193, 1, (1951), 265-275 https://doi.org/10.1016/S0021-9258(19)52451-6
  23. Dali, Seniwati, Abd. Rauf Patong, M. Noor Jalaluddin, Pirman AP, Optimasi produksi enzim lipase dari isolat Aspergillus oryzae pada kopra berjamur, (2010),
  24. Jaeger, Karl-Erich, Stéphane Ransac, Bauke W. Dijkstra, Charles Colson, Margreet van Heuvel, Onno Misset, Bacterial lipases, FEMS Microbiology Reviews, 15, 1, (1994), 29 – 63 https://doi.org/10.1111/j.1574-6976.1994.tb00121.x
  25. Anggoro, Didi Dwi, Faleh Setia Budi, Proses gliserolisis minyak kelapa sawit menjadi mono dan diacyl gliserol dengan pelarut nbutanol dan katalis MgO, Reaktor, 12, 1, (2008), 22-28
  26. Buckle, K.A., R.A. Edwards G.H. Fleet, F.M. Wooton, in, Universitas Indonesia Press. Jakarta, 1985,
  27. Fardiaz, S., Mikrobiologi Pangan 1, Gramedia Pustaka Utama, Jakarta, 1992,
  29. Purwoko, T., Fisiologi Mikroba, 1st ed., Bumi Aksara, Jakarta, 2007,
  30. Pawiroharsono, Suyanto, Penerapan Enzim untuk Penyamakan Kulit Ramah Lingkungan, Jurnal Teknologi Lingkungan, 9, 1, (2008), https://doi.org/10.29122/jtl.v9i1.443

Last update:

No citation recorded.

Last update:

No citation recorded.